Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,450	0	T	G	45.1%	83.2 / 12.1	52	G114G (GGT→GGG)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/26);  new base G (23/0);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.52e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGA  >  NC_000913/2326301‑2326596                                                                                                                                                      |                                                                                                                                                     ccGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTa                                                                                                                                                     <  2:593698/151‑1 (MQ=255) ccGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTa                                                                                                                                                     <  2:900682/151‑1 (MQ=255)     cgcgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATgg                                                                                                                                                  >  1:1047454/1‑150 (MQ=255)      gcgTCAGGCGCTGGTAAAAAGCGGGCTGGCAGAAACGGTGTGCTGATTCACGGTCAATAAAGTATGGGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCGAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTATGGCGGGGGGTATGGa                                                                                                                                                 <  2:94402/150‑1 (MQ=255)      gcgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaa                                                                                                                                                <  2:68017/151‑1 (MQ=255)       cgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaa                                                                                                                                                <  2:122426/150‑1 (MQ=255)        gTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaaa                                                                                                                                              <  1:763507/151‑1 (MQ=255)           aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGCGCATTGTGGCGGGGGGGATGGgaaa                                                                                                                                              >  2:307279/1‑148 (MQ=255)           aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaa                                                                                                                                              >  2:401319/1‑148 (MQ=255)              cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑g                                                                                                                                       <  1:462378/151‑1 (MQ=255)              cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAatat                                                                                                                                           >  2:671998/1‑148 (MQ=255)                   ttAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAATATGT‑Gttt                                                                                                                                    >  2:453146/1‑149 (MQ=255)                     aaaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GTTTAGcc                                                                                                                                >  1:408245/1‑151 (MQ=255)                        aaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGccccc                                                                                                                             <  2:1078011/151‑1 (MQ=255)                          gCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTCAGCCCCCta                                                                                                                           <  1:330002/151‑1 (MQ=255)                            gggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTTGgccc                                                                                                                              >  2:775297/1‑146 (MQ=255)                             ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCtactt                                                                                                                        <  1:141806/151‑1 (MQ=255)                             ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCtactt                                                                                                                        <  1:796039/151‑1 (MQ=255)                                tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GTTTAGCCCCCtactt                                                                                                                        >  1:894888/1‑148 (MQ=255)                                 ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCtacttact                                                                                                                     <  2:605295/150‑1 (MQ=255)                                 ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCtacttac                                                                                                                      <  1:453148/149‑1 (MQ=255)                                   cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGa                                                                                                                 <  1:573935/151‑1 (MQ=255)                                    aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGA‑GTTTAGCCCCCTACTTACT‑CGAt                                                                                                                >  1:1006108/1‑151 (MQ=255)                                                 ggATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCAc                                                                                                      >  1:338859/1‑148 (MQ=255)                                                  gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAACACGctc                                                                                                  >  1:723489/1‑151 (MQ=255)                                                  gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GGTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGc                                                                                                    >  1:172312/1‑149 (MQ=255)                                                         ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGG‑GGTTAGCCCCCTACTTACT‑CCATGCAAAAACACGCCCTGGtt                                                                                             >  1:672907/1‑149 (MQ=255)                                                          gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATc                                                                                          <  1:880315/151‑1 (MQ=255)                                                             aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGG‑GGTTAGCCCCCCCCCTACT‑CGATGCAAAAACACGCCCTgg                                                                                               >  2:797223/1‑143 (MQ=255)                                                                aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCtt                                                                                     <  2:513808/150‑1 (MQ=255)                                                                aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCtt                                                                                     <  1:814638/150‑1 (MQ=255)                                                                    tATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAAATGA‑GTTTTCGCCCCTCCTTATT‑CGGTGCAAAAGAAAGCCCTGGTTATTGTTTTgg                                                                                   >  1:135058/1‑148 (MQ=255)                                                                            tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAg                                                                         <  1:948176/150‑1 (MQ=255)                                                                             cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGt                                                                       <  1:217406/151‑1 (MQ=255)                                                                             cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGTAAAAAGG‑GTTTAGACCCCCACTTTCT‑CGCTGCAAAAACCCGCTCTTGTGATCGGCtttg                                                                                   >  1:734250/1‑137 (MQ=255)                                                                                      aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAAAACGCTCTGGGTTTCGTCTTTGGGACGGGCAGGGTTAt                                                                   >  2:99563/1‑146 (MQ=255)                                                                                       aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTa                                                             <  2:528763/151‑1 (MQ=255)                                                                                        aGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGA‑GTTTATGCCCCCACTTACC‑TGATGGAAAAGCACGCCCTTGTTATCGTCTTTGAGACGGGCCGGTTTATTAc                                                                >  1:504987/1‑147 (MQ=255)                                                                                           gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATTGGAAATATGAGGTTTTGCCCCCT‑CTTACT‑CGAAGTAAAAACACGCCCTGGTTTTTCTTTTTGCGAAGGACAGGgtttttacacaa                                                            >  2:700178/1‑139 (MQ=255)                                                                                              gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATTA‑GTTTAGCCCCCCACTTTCT‑CGGTGCAAAAGCACGCTCTGGGTATCGTCTTTGGGACGGACAGGGTTATTACGGAAAcc                                                         >  2:221558/1‑148 (MQ=255)                                                                                                gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GGTTAGCCCCCCACTTAAT‑CGATGCAAAAGCACGCTCTGGGTATCGTCTTGGGGACGGACAGGGTTATGACGTAATCCTgcgc                                                    >  1:985231/1‑151 (MQ=255)                                                                                                    gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATg                                                <  2:972421/151‑1 (MQ=255)                                                                                                           aGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGAt                                         <  1:672001/151‑1 (MQ=255)                                                                                                            ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GGTTAGCCCCCTACTTACT‑CGATGCAAAAACACGCTCTGGTTATTGTCTTGGAGACGGACAGGGTTATGACGTAATCCTGCGCGATGGcc                                             >  1:381979/1‑146 (MQ=255)                                                                                                                   caggcaggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGcc                                  <  1:583263/150‑1 (MQ=255)                                                                                                                       caggTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAATATGA‑GTTTATCCCCCCACTTACTCCGAGGCAAAA‑CACGCCCTGGCTATCGTCCTGGAAATGGACAAGGTTATGAAGTAATCCTTGGCGCTTGCCTTCTGTGGGCCAacc                              >  1:1009186/1‑150 (MQ=255)                                                                                                                        cggTCAGGCGCAGAGCATTGTGGCGGCGGGCCCTGAAAATATTG‑GTTTAGCCCCCCACTTACT‑CGACGCCAAACCACCCCCTGGTTCTCGTCTTCGGGACGGACAGGTTTTTGACGTAATCCCGCGCGATGGCCCGCTGGGCGcccccc                              >  2:136614/2‑149 (MQ=255)                                                                                                                         ggTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGG‑GGTTAGCCCCCTACTTACT‑CGATGCAAAAGAAAGCTCTGGTTATCGGCTTGGAGAAGGACAAGGTTATGACGTAAAACTGCGCGATGGCCTGGTGGGCGCCCCCCATg                           >  2:25537/1‑151 (MQ=255)                                                                                                                              ggCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGA‑GTTTAGCCCCCTACTTACC‑CGATGCAAAAACACGCTCTGGGTTTTGGCTTGGGGACGGACAGGTTTTTTAAGTAATCCCGCGCGATGGCCTGAGTTGGGCCACCCATGGTTAt                      >  1:19171/1‑151 (MQ=255)                                                                                                                                 gcAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCat                   <  1:419781/151‑1 (MQ=255)                                                                                                                                   agagCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCatat                 <  1:912363/151‑1 (MQ=255)                                                                                                                                                  ggggTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGa  <  1:65719/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGA‑GTTTAGCCCCCTACTTACT‑CGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGA  >  NC_000913/2326301‑2326596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑