Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,4500TG45.1% 83.2 / 12.1 52G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/26);  new base G (23/0);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.52e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGA  >  NC_000913/2326301‑2326596
                                                                                                                                                     |                                                                                                                                                    
ccGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTa                                                                                                                                                     <  2:593698/151‑1 (MQ=255)
ccGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTa                                                                                                                                                     <  2:900682/151‑1 (MQ=255)
    cgcgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATgg                                                                                                                                                  >  1:1047454/1‑150 (MQ=255)
     gcgTCAGGCGCTGGTAAAAAGCGGGCTGGCAGAAACGGTGTGCTGATTCACGGTCAATAAAGTATGGGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCGAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTATGGCGGGGGGTATGGa                                                                                                                                                 <  2:94402/150‑1 (MQ=255)
     gcgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaa                                                                                                                                                <  2:68017/151‑1 (MQ=255)
      cgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaa                                                                                                                                                <  2:122426/150‑1 (MQ=255)
       gTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaaa                                                                                                                                              <  1:763507/151‑1 (MQ=255)
          aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGCGCATTGTGGCGGGGGGGATGGgaaa                                                                                                                                              >  2:307279/1‑148 (MQ=255)
          aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaa                                                                                                                                              >  2:401319/1‑148 (MQ=255)
             cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAg                                                                                                                                       <  1:462378/151‑1 (MQ=255)
             cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAatat                                                                                                                                           >  2:671998/1‑148 (MQ=255)
                  ttAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAATATGTGttt                                                                                                                                    >  2:453146/1‑149 (MQ=255)
                    aaaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGcc                                                                                                                                >  1:408245/1‑151 (MQ=255)
                       aaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccccc                                                                                                                             <  2:1078011/151‑1 (MQ=255)
                         gCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTCAGCCCCCta                                                                                                                           <  1:330002/151‑1 (MQ=255)
                           gggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTTGgccc                                                                                                                              >  2:775297/1‑146 (MQ=255)
                            ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCtactt                                                                                                                        <  1:141806/151‑1 (MQ=255)
                            ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCtactt                                                                                                                        <  1:796039/151‑1 (MQ=255)
                               tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCtactt                                                                                                                        >  1:894888/1‑148 (MQ=255)
                                ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCtacttact                                                                                                                     <  2:605295/150‑1 (MQ=255)
                                ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCtacttac                                                                                                                      <  1:453148/149‑1 (MQ=255)
                                  cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGa                                                                                                                 <  1:573935/151‑1 (MQ=255)
                                   aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCTACTTACTCGAt                                                                                                                >  1:1006108/1‑151 (MQ=255)
                                                ggATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                                                                                      >  1:338859/1‑148 (MQ=255)
                                                 gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGctc                                                                                                  >  1:723489/1‑151 (MQ=255)
                                                 gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                                                                                    >  1:172312/1‑149 (MQ=255)
                                                        ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGGGGTTAGCCCCCTACTTACTCCATGCAAAAACACGCCCTGGtt                                                                                             >  1:672907/1‑149 (MQ=255)
                                                         gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                                                                                          <  1:880315/151‑1 (MQ=255)
                                                            aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGGGGTTAGCCCCCCCCCTACTCGATGCAAAAACACGCCCTgg                                                                                               >  2:797223/1‑143 (MQ=255)
                                                               aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCtt                                                                                     <  2:513808/150‑1 (MQ=255)
                                                               aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCtt                                                                                     <  1:814638/150‑1 (MQ=255)
                                                                   tATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAAATGAGTTTTCGCCCCTCCTTATTCGGTGCAAAAGAAAGCCCTGGTTATTGTTTTgg                                                                                   >  1:135058/1‑148 (MQ=255)
                                                                           tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAg                                                                         <  1:948176/150‑1 (MQ=255)
                                                                            cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGt                                                                       <  1:217406/151‑1 (MQ=255)
                                                                            cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGTAAAAAGGGTTTAGACCCCCACTTTCTCGCTGCAAAAACCCGCTCTTGTGATCGGCtttg                                                                                   >  1:734250/1‑137 (MQ=255)
                                                                                     aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCTACTTACTCGATGCAAAAAAACGCTCTGGGTTTCGTCTTTGGGACGGGCAGGGTTAt                                                                   >  2:99563/1‑146 (MQ=255)
                                                                                      aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTa                                                             <  2:528763/151‑1 (MQ=255)
                                                                                       aGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTATGCCCCCACTTACCTGATGGAAAAGCACGCCCTTGTTATCGTCTTTGAGACGGGCCGGTTTATTAc                                                                >  1:504987/1‑147 (MQ=255)
                                                                                          gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATTGGAAATATGAGGTTTTGCCCCCTCTTACTCGAAGTAAAAACACGCCCTGGTTTTTCTTTTTGCGAAGGACAGGgtttttacacaa                                                            >  2:700178/1‑139 (MQ=255)
                                                                                             gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATTAGTTTAGCCCCCCACTTTCTCGGTGCAAAAGCACGCTCTGGGTATCGTCTTTGGGACGGACAGGGTTATTACGGAAAcc                                                         >  2:221558/1‑148 (MQ=255)
                                                                                               gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTAGCCCCCCACTTAATCGATGCAAAAGCACGCTCTGGGTATCGTCTTGGGGACGGACAGGGTTATGACGTAATCCTgcgc                                                    >  1:985231/1‑151 (MQ=255)
                                                                                                   gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATg                                                <  2:972421/151‑1 (MQ=255)
                                                                                                          aGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGAt                                         <  1:672001/151‑1 (MQ=255)
                                                                                                           ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATTGTCTTGGAGACGGACAGGGTTATGACGTAATCCTGCGCGATGGcc                                             >  1:381979/1‑146 (MQ=255)
                                                                                                                  caggcaggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGcc                                  <  1:583263/150‑1 (MQ=255)
                                                                                                                      caggTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAATATGAGTTTATCCCCCCACTTACTCCGAGGCAAAACACGCCCTGGCTATCGTCCTGGAAATGGACAAGGTTATGAAGTAATCCTTGGCGCTTGCCTTCTGTGGGCCAacc                              >  1:1009186/1‑150 (MQ=255)
                                                                                                                       cggTCAGGCGCAGAGCATTGTGGCGGCGGGCCCTGAAAATATTGGTTTAGCCCCCCACTTACTCGACGCCAAACCACCCCCTGGTTCTCGTCTTCGGGACGGACAGGTTTTTGACGTAATCCCGCGCGATGGCCCGCTGGGCGcccccc                              >  2:136614/2‑149 (MQ=255)
                                                                                                                        ggTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGGGGTTAGCCCCCTACTTACTCGATGCAAAAGAAAGCTCTGGTTATCGGCTTGGAGAAGGACAAGGTTATGACGTAAAACTGCGCGATGGCCTGGTGGGCGCCCCCCATg                           >  2:25537/1‑151 (MQ=255)
                                                                                                                             ggCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCTACTTACCCGATGCAAAAACACGCTCTGGGTTTTGGCTTGGGGACGGACAGGTTTTTTAAGTAATCCCGCGCGATGGCCTGAGTTGGGCCACCCATGGTTAt                      >  1:19171/1‑151 (MQ=255)
                                                                                                                                gcAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCat                   <  1:419781/151‑1 (MQ=255)
                                                                                                                                  agagCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCatat                 <  1:912363/151‑1 (MQ=255)
                                                                                                                                                 ggggTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGa  <  1:65719/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGA  >  NC_000913/2326301‑2326596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: