Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,328	0	C	A	41.7%	120.5 / 39.0	72	intergenic (+337/‑76)	yahL/yahM	uncharacterized protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (40/2);  new base A (0/30);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 3.02e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATT  >  NC_000913/345185‑345474                                                                                                                                                 |                                                                                                                                                   tGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAAcc                                                                                                                                             >  2:71604/1‑151 (MQ=255)   aTCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGc                                                                                                                                           >  1:1063134/1‑151 (MQ=255)    tctcATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGcc                                                                                                                                          >  1:258677/1‑151 (MQ=255)        aTGCAGATGTTTTGTGAATGTGTTGGTTGATTTTCATATGAAAAAAATCATAATCCCATCATTTTGGTGTGGGTTTTTTTTTGTATCTTACGCACC‑GCACCCAAGCCTAACAAAAAATATGCTGTAAGCCTCATATCAAAAACCGCCAt                                                                                                                                        <  2:580564/149‑1 (MQ=255)          gCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGc                                                                                                                                    >  2:146051/1‑151 (MQ=255)          gCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGc                                                                                                                                    >  2:146033/1‑151 (MQ=255)           cAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCt                                                                                                                                   >  2:333298/1‑151 (MQ=255)            aGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCtc                                                                                                                                  >  1:337192/1‑151 (MQ=255)               tGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCtcatc                                                                                                                               >  1:613646/1‑151 (MQ=255)                  tttGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAgg                                                                                                                            >  1:202410/1‑151 (MQ=255)                         aTGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGc                                                                                                                      >  1:619133/1‑150 (MQ=255)                          tgtgTTGGTTGACATTAATATAAAAAAAATCATAATCCCATCATGTTTGTGGGGGTTTTTTTTTGTATCTTACGCACC‑CCACTAAAGCCTGACAAAAAATATGCTGAAAGGCTCATAAAAAAAACCGCCATTAGCTCATCAGGAaga                                                                                                                        <  1:875412/147‑1 (MQ=255)                           gtgtTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGc                                                                                                                      >  1:397491/1‑148 (MQ=255)                              ttgttttACATTAATATAAAAAAAATCATAATCCCATCATGTTTGTGTGGGTTTTTTTTTGAATCTTCCGAACC‑CCCCCCAAGCCGAAAAAAAAATATGCTGAAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                 <  2:200156/150‑1 (MQ=255)                              ttggttgACATTAATATAAAAAAAATCATAATCCCATCATGTTTGTGGGGGTTTTTTTCTGTATCTTACGCACC‑GCACTAAAGCCTGACAAAAAATATGCTTAAGGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAg                                                                                                                    <  1:907850/147‑1 (MQ=255)                               tggttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacgac                                                                                                               >  1:882954/1‑151 (MQ=255)                                  ttgACATTCATAAAAAAAAAATCATAATCCCATCATTTTTGTGTGGGGTTTTTTTTGTATCTTACCCACC‑GCACCCACCCCTAACAAAAAATATGCTGTAGGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                 <  2:338337/146‑1 (MQ=255)                                   tgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc                                                                                                           >  2:304503/1‑151 (MQ=255)                                    gACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGGGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc                                                                                                           >  2:763570/1‑150 (MQ=255)                                       aTTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAgg                                                                                                        >  2:32022/1‑150 (MQ=255)                                           atatAAAAAAAATAATAATCCCATCTTGTTGGTGGGGGTTTTTTTCTGTATCTTACGCACC‑GCACTCAAGCCTAACAAAAAATATGCTGTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt                                                                                                     <  1:133992/149‑1 (MQ=255)                                           ataaaaaaaaaaTCATAATCCCATCATGTTTGTGTGGGTTCTTTTCTGTATCTTCCGCACC‑GCACTCAAGCCTAAAAAAAAATATGCTGTAAGGCTAAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt                                                                                                     <  1:707026/144‑1 (MQ=255)                                             ataaaaaaaaTCATAATCCCATCATTTTGGTGTGGGGTTTTTTCTGTATCTTCCCCACC‑CCACTCAACCCTAAAAAAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt                                                                                                      <  1:865640/143‑1 (MQ=255)                                                aaaaaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTAt                                                                                              >  1:1052138/1‑151 (MQ=255)                                                aaaaaaaTCATAATCCCATCATTTTTGTGGGGGGTCTTTTCTGTATCTTCCGCACC‑GCACCCAAGCCTGACAAAAAATATGCTGTAAGGCTAATACAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTAt                                                                                              <  1:788327/151‑1 (MQ=255)                                                aaaaaaaCAATAATGCCATGTTGTTATTGGGGGTTTTTTTTTGAATGTT‑GGCAACGGCACTCACGCTCAACAAAAAAAATTTTGCAAGGCTTATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt                                                                                                     <  1:411891/144‑1 (MQ=255)                                                   aaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGt                                                                                           >  1:728479/1‑151 (MQ=255)                                                   aaaaTAATAATCCCATAATGTTGGTGGGGGTTCTTTTCTTTATCTTCCGCCCC‑GCACCAAGGCCTAACAAAAAATATGCTGGAAGCCTCAAACAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGt                                                                                           <  1:236859/151‑1 (MQ=255)                                                            aTTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTc                                                                                  >  1:66275/1‑151 (MQ=255)                                                               ccATCTTTTTTGTGGGGGGTTTTTTCTGTATCTTCCGCCCC‑GCACCCAAGCCTGAAAAAAAATATGCGGTAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt                                                                                   <  2:254114/147‑1 (MQ=255)                                                               ccATCATTTTTGTGTGGGTTTTTTTCTTTATCTTACGCATC‑GCACTCAAGCCTGAAAAAAAATATGCTGTAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt                                                                                   <  1:146009/147‑1 (MQ=255)                                                                catcatGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAgg                                                                              >  2:59472/1‑151 (MQ=255)                                                                  tattGTTGGTGGGGTGTTTTTTTTTTTTCTTACCCATC‑CCACCCAACCCAGAAAAAAAATATGCTAAAAGCCTAATATAAAAATCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACggg                                                                                      <  2:731565/138‑1 (MQ=255)                                                                   catGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGcc                                                                            >  2:145756/1‑150 (MQ=255)                                                                     tttttGTGGGGGTTTTTTTCTGTTTTTACCCCACC‑GCACTCAAGCTTAAAAAAAAATTTGTTGAAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                           <  1:781440/147‑1 (MQ=255)                                                                     tGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCg                                                                         >  2:194702/1‑151 (MQ=255)                                                                       tttGTGTGGGTTTTTTTTTGTATCTTCCGCACC‑GCCCCAAACCCGAAAAAAAAAAATGTTGTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                           <  2:485561/147‑1 (MQ=255)                                                                       tttGTGTGGGTTTTTTTCTGTATCTTACGCACC‑GCACTCAAGCCTGAAAAAAAATATGCTGTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGa                                                                        <  1:147901/150‑1 (MQ=255)                                                                       tttGTGTGGGTTCTTTTCTGTATCTTACGCACC‑GCACTCAAGCCTGAAAAAAAATATGCTGAAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTc                                                                          <  1:717183/148‑1 (MQ=255)                                                                       tttGTGTGGGGTTTTTTCTGTATTTTACGCACC‑GCACACAAGCCTGAAAAAAAATATGCTGTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGa                                                                        <  1:271406/150‑1 (MQ=255)                                                                       tttGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGAt                                                                       >  2:768772/1‑151 (MQ=255)                                                                       tttGTGGGGTGTTTTCTCTGTTTCTCACACACC‑CACCTCAGCCCGGACAAAAAATAGGCGGTAAGTCTAAAAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAgg                                                                              <  2:1002367/144‑1 (MQ=255)                                                                        tttggtgGGGTTTTTTCTTTATCTTCCTCACC‑CCACTCAACCCTAACAAAAAATATGTTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGAt                                                                       <  2:223854/146‑1 (MQ=255)                                                                                ggTTTTTTCTGTATCTTCCCCACC‑GCACCCAAGCCTGACAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCa                                                              <  1:261956/151‑1 (MQ=255)                                                                                        cTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTc                                                       >  2:1045445/1‑150 (MQ=255)                                                                                                agcgaacc‑GCACCCAAGCCGGAAAAAAAATATCGTGTAGCCCCCATACAAAAATCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAAct                                                 <  1:912096/141‑1 (MQ=255)                                                                                                   gCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTg                                           >  1:992814/1‑151 (MQ=255)                                                                                                       c‑GCACTCAAGCCTGACAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                       <  1:971346/151‑1 (MQ=255)                                                                                                            caaaaGCCTAAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGAt                                   <  2:143692/148‑1 (MQ=255)                                                                                                            cTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                       >  2:228187/1‑147 (MQ=255)                                                                                                              cAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcc                                 >  2:461734/1‑151 (MQ=255)                                                                                                               aaGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATccc                                >  1:55307/1‑151 (MQ=255)                                                                                                                 gCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCt                              >  2:416654/1‑151 (MQ=255)                                                                                                                  ccTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCtt                             >  2:703443/1‑151 (MQ=255)                                                                                                                  ccTGAAAAAAAATATGCTATAAGCCTAAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAg                                     <  1:67083/143‑1 (MQ=255)                                                                                                                      aaaGAAAATATGCTGTAGGTCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttat                           <  1:71604/147‑1 (MQ=255)                                                                                                                       cAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                        >  2:445397/1‑151 (MQ=255)                                                                                                                        aaaaaaTATGCTGTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatc                       <  1:404674/149‑1 (MQ=255)                                                                                                                        aGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatc                       >  2:330676/1‑151 (MQ=255)                                                                                                                          aaaaTATGTTGAAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcat                     <  1:32022/151‑1 (MQ=255)                                                                                                                          aaaaTATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcat                     >  1:964241/1‑151 (MQ=255)                                                                                                                             atatGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATccc                  >  2:514088/1‑151 (MQ=255)                                                                                                                                tGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTg                <  2:1045406/150‑1 (MQ=255)                                                                                                                                  cTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa              >  2:1047662/1‑150 (MQ=255)                                                                                                                                  cTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa              >  2:945316/1‑150 (MQ=255)                                                                                                                                   tGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGATCGAGGCCCCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGACCCCCTTATTATCATCCCTGCaa             >  2:696786/1‑150 (MQ=255)                                                                                                                                    gTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaa             >  2:625980/1‑149 (MQ=255)                                                                                                                                      aaGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGTaaa            <  1:146034/148‑1 (MQ=255)                                                                                                                                      aaGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaaa            <  1:146052/148‑1 (MQ=255)                                                                                                                                       aGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTgg        >  1:940148/1‑151 (MQ=255)                                                                                                                                          cTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAAc     >  2:756398/1‑151 (MQ=255)                                                                                                                                             atatCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACAtt  >  1:1069842/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATC‑GCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATT  >  NC_000913/345185‑345474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑