| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 809,340 | C→G | intergenic (‑83/‑4) | bioA ← / → bioB | 7,8‑diaminopelargonic acid synthase, PLP‑dependent/biotin synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 809,340 | 0 | C | G | 98.3% | 227.4 / ‑2.7 | 59 | intergenic (‑83/‑4) | bioA/bioB | 7,8‑diaminopelargonic acid synthase, PLP‑dependent/biotin synthase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (34/24); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
GGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCG > NC_000913/809195‑809484 | ggTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCAt > 2:429004/1‑151 (MQ=255) gTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATg > 2:196584/1‑151 (MQ=255) tCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATgg > 1:10274/1‑151 (MQ=255) tCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATgg > 1:972176/1‑151 (MQ=255) cATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGc > 2:206659/1‑151 (MQ=255) ggATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTc < 1:977656/150‑1 (MQ=255) aTGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCAcc > 1:413943/1‑151 (MQ=255) tgtgTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCg > 1:1078505/1‑151 (MQ=255) aTGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCAc < 1:769289/151‑1 (MQ=255) aTGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAAATTTAGGTTTACAAGTCTACACCGAATTAACTATAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACTGCCTAc > 2:397736/1‑151 (MQ=255) tGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCa > 1:478737/1‑149 (MQ=255) gATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACAtt < 1:398355/151‑1 (MQ=255) atGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAATAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTc < 1:396207/151‑1 (MQ=255) cGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCa > 1:526702/1‑150 (MQ=255) gTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCa > 1:569850/1‑149 (MQ=255) ggTCAAAGGCAAGATCGTCCGTTGCCATAATCGACTTGTAAACCAAATGGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGt < 2:887203/149‑1 (MQ=255) cAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTcac > 1:809382/1‑149 (MQ=255) cAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAg < 2:844854/151‑1 (MQ=255) aaGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGa > 1:815042/1‑150 (MQ=255) aGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGaa > 1:897652/1‑150 (MQ=255) atgtgcGTCCGGTGTCAAAATCGACGTGTAAGCCAAATTGAAAAGAGTTAGGTTTACAAGTCTACACCGAATTAACCACAAAAAACACGTTGTGGAGAAGGCCCATGGCTCACCGACCACGAATGACATTGACGCAAGTCACAGActtatt > 2:113217/6‑151 (MQ=255) tCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGaaaa > 1:451943/1‑150 (MQ=255) cGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAAcc > 1:865341/1‑151 (MQ=255) gTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGtt < 2:974998/150‑1 (MQ=255) cATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCt > 1:839727/1‑151 (MQ=255) cATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCt < 2:337346/151‑1 (MQ=255) tCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATc > 1:967277/1‑151 (MQ=255) cGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATct > 2:393574/1‑151 (MQ=255) cGACTTGTAAAACAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCCCCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATc < 2:843327/150‑1 (MQ=255) gACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATctg < 1:467647/151‑1 (MQ=255) aCTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATctg > 2:1061192/1‑150 (MQ=255) aaaCCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTg > 2:297788/1‑151 (MQ=255) ccAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAg < 1:490352/151‑1 (MQ=255) cAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgc > 1:450398/1‑151 (MQ=255) aaTTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgcg > 2:120411/1‑150 (MQ=255) tGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCgcagc > 1:540133/1‑151 (MQ=255) gAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCgcagca < 2:413941/151‑1 (MQ=255) gAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCgcagc > 1:93338/1‑150 (MQ=255) aaGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGt < 1:218385/151‑1 (MQ=255) aGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTg < 2:508079/151‑1 (MQ=255) ttAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATc > 1:568722/1‑151 (MQ=255) aGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCa < 1:1016599/147‑1 (MQ=255) gTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGcc > 2:504093/1‑150 (MQ=255) tttACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGcc > 2:862472/1‑149 (MQ=255) ttACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGc > 1:874698/1‑151 (MQ=255) tACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGc < 2:102272/150‑1 (MQ=255) aCAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCAt < 1:274551/151‑1 (MQ=255) cAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCAtt > 1:168868/1‑151 (MQ=255) ccGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCg > 2:227733/1‑151 (MQ=255) ttAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAgg > 1:403683/1‑151 (MQ=255) aacaaAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAg < 1:518755/151‑1 (MQ=255) aaaaCACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAg < 1:835790/151‑1 (MQ=255) aaCACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGc > 1:409201/1‑150 (MQ=255) aaCACGATTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAATCCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCa < 2:10274/151‑1 (MQ=255) acacGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCAc < 2:968842/151‑1 (MQ=255) acGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGt > 2:346673/1‑151 (MQ=255) tttGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGc < 2:451941/150‑1 (MQ=255) ggAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGt < 1:196586/150‑1 (MQ=255) gagaAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCg < 2:634725/151‑1 (MQ=255) | GGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCG > NC_000913/809195‑809484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |