| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,433 | 0 | G | C | 57.1% | 13.6 / 51.3 | 35 | intergenic (+604/+132) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base G (2/13); new base C (20/0); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-06 | |||||||||||
GTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTC > NC_000913/1097284‑1097488 | gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCg < 1:1197554/150‑1 (MQ=33) ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTG‑ACTCCCCTCCCTCGCCCCCCGGGCAGCCCCCTccc > 1:1229123/1‑149 (MQ=11) ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGTGGGAAGTCGC‑CCGCTCCCCCTGCGGGGAGCCCCCCCAc > 1:894240/1‑151 (MQ=11) cAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACt < 2:725904/151‑1 (MQ=33) gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTCCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGc < 1:933466/151‑1 (MQ=18) gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑ACTCGCTTCGCTCCCCCTGCGGGCAGCCCCCCCAc > 2:770695/1‑148 (MQ=255) gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGc < 1:1041633/151‑1 (MQ=33) tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGTGGGGGGGGTTG‑TCTCCCTTCGCTCCCCCTGCGGGGAGACCGcccccctcg > 2:1195606/1‑142 (MQ=11) tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTG‑AATCTCCTCGCCCGCCCTGCGGGGAACCCCCTcccctcgt > 2:107269/1‑144 (MQ=11) cGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTCCAATATACGCAAAAAAAGCCCGTTCTGTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGtt < 1:744621/149‑1 (MQ=255) cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑ACTCCCCTCGCTCGCCCCGCGGGCCGCCCCCTCCCTGCGTTTAccg > 2:482647/1‑147 (MQ=11) cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTG‑GCTCCCCTCCCTCGCCCCCCGGGGCACCCCCCCCCTGCGTTCCCGGt > 2:208735/1‑150 (MQ=11) ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGTTG‑ACTCGCTTCGCCCGCCCCGCGGGCAGCCCCCCCACTGCGTTCAc > 2:1029300/1‑146 (MQ=11) cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGCGGGGGGGATTG‑AGTCACTTCCCCCTCCCCCCGGGGCGCCCCCCCACTTCGTTCACgg > 2:433023/1‑147 (MQ=11) ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTT‑GCTCCCCTCGCTCGCCCTGCCGGCACCGCCCTCCCTggggtcgc > 1:1099047/1‑137 (MQ=11) ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTG‑ACTCCCCCCGCTCGCCCTCCGGGCAGCCCCCTCCCGGGGTTCTCCGTccg > 1:420145/1‑148 (MQ=11) gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGATG‑ACTCCCTTTGCTTGCCCCCCGGGGAGCCCCCTCACCGCGtttcagggccgt > 2:1201929/1‑140 (MQ=11) tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTT‑AGTCGCTTCCCTCCCCCCGCGGGGAGGCCCCCCTCCGCGTTCTCGGTCTGTcc > 2:868478/1‑151 (MQ=11) cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa < 2:914130/151‑1 (MQ=31) cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCCCTTTGCTCCCCCTGCGGGGAGCCCCCCCCCTGCGGTCACGGGCTggtcc > 2:1007711/1‑147 (MQ=11) ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCaa < 2:539150/151‑1 (MQ=31) gCTTCTCATCCCCCCGTTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTg < 2:1084875/151‑1 (MQ=14) cTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATT‑ACTCGCTTCCCTCGCCCTGCGGGCCGCCCCCCCCCTGCGTTCACGGGCTGTCCCACTgg > 2:154569/1‑151 (MQ=11) ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTG‑ACTCGCCTCGCTCCCCCTCCGGGCACCCCCCTCCCCGCGGTTCCCGTCATTCCAACTgg > 2:414058/1‑150 (MQ=11) cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑AATCCCCTCCCTCGCCCCTCGGGGAGGCCCCTCCCCGCGTTTCCGGTCTTTCCCAcctg > 2:251292/1‑143 (MQ=11) cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCa < 1:1195599/151‑1 (MQ=31) gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTG‑AATCGCTTCGCTCGCCCCGCGGGGCGCCCCCTCCCTGCGTTTACGGTCTGTCCAACTGGCTGTCGCCCGTTGTC‑Gaa > 2:536071/1‑150 (MQ=11) gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAAc < 2:15968/151‑1 (MQ=31) gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAAccc < 2:679086/151‑1 (MQ=31) gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAAcc < 2:52487/150‑1 (MQ=31) aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCCGCCGGCCGCCCCCCCACTGCGTTCCCGGTTTGTCCCACCGGCTGTCGCCCGGTGTCGGAAcccc > 2:939755/1‑151 (MQ=11) atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑AATCGCTTCGCTCGCCCTGCGGGCAGCCCCCCCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCCGTTGTC‑GAAcccc > 2:852147/1‑149 (MQ=9) tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGCGGGGGGGGTTG‑ACCTCCTTCGCTCTCCCTGCGGGCCACCCCCTCACTGCGGTTACCATCTTTCCCACTCGCGGTCGccccttttccaccccc > 2:952966/1‑134 (MQ=2) tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTT‑AATAGCCTCCCCCCCCCCGCTGGCAGCCCGCGCACTCCGGTTATGCGCTGTCCCACCCGCCCTCGCCCGTTGTcccccccc > 1:369658/1‑141 (MQ=2) ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTc < 2:1084988/151‑1 (MQ=31) ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTc < 2:937936/151‑1 (MQ=31) | GTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTG‑ACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTC > NC_000913/1097284‑1097488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |