Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,378	G→T	R218S (CGT→AGT)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,378	0	G	T	100.0%	290.7 / NA	71	R218S (CGT→AGT)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (43/28);  total (43/28)

CAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCC  >  NC_000913/3440231‑3440525                                                                                                                                                    |                                                                                                                                                    cAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAAt                                                                                                                                                  >  2:1163140/1‑151 (MQ=255) cAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAAt                                                                                                                                                  >  2:292459/1‑151 (MQ=255)     gCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGcc                                                                                                                                              <  2:134545/151‑1 (MQ=255)     gCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGcc                                                                                                                                              >  2:655821/1‑151 (MQ=255)            gTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTc                                                                                                                                         >  1:633109/1‑149 (MQ=255)            gTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAg                                                                                                                                       <  2:15904/151‑1 (MQ=255)                aTTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATc                                                                                                                                   <  2:612194/151‑1 (MQ=255)                aTTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATc                                                                                                                                   <  1:1138915/151‑1 (MQ=255)                 ttCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCg                                                                                                                                  >  2:770032/1‑151 (MQ=255)                   ccAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGa                                                                                                                                 <  1:1191915/150‑1 (MQ=255)                   ccAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGa                                                                                                                                 <  2:148614/150‑1 (MQ=255)                            cAACAGGGCGCAGCAGGATCGGATTGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGt                                                                                                                        <  1:806001/150‑1 (MQ=255)                             aaCAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTg                                                                                                                      <  1:1179654/151‑1 (MQ=255)                                aGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGtt                                                                                                                   <  2:592055/151‑1 (MQ=255)                                   gcgcAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTcc                                                                                                                >  2:822589/1‑151 (MQ=255)                                    cgcAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCa                                                                                                               <  2:456265/151‑1 (MQ=255)                                            gatcggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATg                                                                                                       >  2:935334/1‑151 (MQ=255)                                                 gatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAg                                                                                                  <  1:19310/151‑1 (MQ=255)                                                 gatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAg                                                                                                  <  1:497830/151‑1 (MQ=255)                                                  atcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGc                                                                                                 >  2:1015434/1‑151 (MQ=255)                                                    cgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATtgt                                                                                                                             >  1:355726/1‑121 (MQ=255)                                                    cgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATtgt                                                                                                                             <  2:355727/121‑1 (MQ=255)                                                    cgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCtt                                                                                               <  2:834266/151‑1 (MQ=255)                                                     gAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTg                                                                                              <  1:824902/151‑1 (MQ=255)                                                         tctGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTcc                                                                                           >  2:67355/1‑150 (MQ=255)                                                            ggTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCa                                                                                          <  1:849976/148‑1 (MQ=255)                                                             gTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTc                                                                                      >  1:579871/1‑151 (MQ=255)                                                               ttCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCgg                                                                                    >  2:511971/1‑151 (MQ=255)                                                                tctcTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGt                                                                                   >  1:350953/1‑151 (MQ=255)                                                                     tcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCt                                                                              >  2:120409/1‑151 (MQ=255)                                                                       tttCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGt                                                                            >  2:352816/1‑151 (MQ=255)                                                                        ttCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTg                                                                             <  1:1055508/149‑1 (MQ=255)                                                                        ttCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGtt                                                                           >  2:766388/1‑151 (MQ=255)                                                                         tCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTc                                                                          >  1:1102548/1‑151 (MQ=255)                                                                         tCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTc                                                                          >  2:802081/1‑151 (MQ=255)                                                                             ttCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTac                                                                       >  2:778199/1‑150 (MQ=255)                                                                             ttCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTac                                                                       >  2:524727/1‑150 (MQ=255)                                                                              tCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTaca                                                                      <  1:731050/150‑1 (MQ=255)                                                                               cAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACAcg                                                                    >  1:172502/1‑151 (MQ=255)                                                                                 ggCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACAcgc                                                                   >  1:1143241/1‑150 (MQ=255)                                                                                         tACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTc                                                           >  2:102806/1‑150 (MQ=255)                                                                                           cATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAAc                                                        <  1:681274/151‑1 (MQ=255)                                                                                            aTCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAAc                                                        >  2:66988/1‑150 (MQ=255)                                                                                             tCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTCCGCTGTTCTACACGCGCTGCTTCAACAt                                                      >  2:282999/1‑151 (MQ=255)                                                                                              cACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACAtt                                                     >  1:1233438/1‑151 (MQ=255)                                                                                                cGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGt                                                   >  2:136207/1‑151 (MQ=255)                                                                                                       cAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCaa                                             <  1:892113/150‑1 (MQ=255)                                                                                                       cAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAAt                                            <  1:1007708/151‑1 (MQ=255)                                                                                                       cAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAAt                                            >  2:872411/1‑151 (MQ=255)                                                                                                       cAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAAt                                            >  1:702686/1‑151 (MQ=255)                                                                                                        aaCGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATa                                           <  2:379880/151‑1 (MQ=255)                                                                                                         acgaaacagtCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATAc                                          <  1:347921/142‑1 (MQ=255)                                                                                                           gAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGc                                        >  1:909073/1‑151 (MQ=255)                                                                                                               gCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCca                                    <  1:168912/151‑1 (MQ=255)                                                                                                                cTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCcac                                   >  2:1029073/1‑151 (MQ=255)                                                                                                                   ccAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAgg                                >  1:337384/1‑151 (MQ=255)                                                                                                                        tgttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTg                            >  2:321661/1‑150 (MQ=255)                                                                                                                        tgttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGt                           >  2:40249/1‑151 (MQ=255)                                                                                                                         gttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGt                           <  1:594337/150‑1 (MQ=255)                                                                                                                         gttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGt                           >  1:23553/1‑150 (MQ=255)                                                                                                                         gttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGt                           <  2:329820/150‑1 (MQ=255)                                                                                                                         gttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTa                          <  2:898977/151‑1 (MQ=255)                                                                                                                          ttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGt                           >  1:554136/1‑149 (MQ=255)                                                                                                                             aGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCAt                      <  1:1247779/151‑1 (MQ=255)                                                                                                                              gCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATg                     >  1:1182331/1‑151 (MQ=255)                                                                                                                               ccAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGc                    >  2:589496/1‑151 (MQ=255)                                                                                                                                           ccGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcagc          >  1:1088072/1‑149 (MQ=255)                                                                                                                                           ccGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcagc          >  1:1046969/1‑149 (MQ=255)                                                                                                                                           ccGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcagc          >  2:225207/1‑149 (MQ=255)                                                                                                                                             gCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGAc      >  1:893456/1‑151 (MQ=255)                                                                                                                                              cACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACg     >  1:546607/1‑151 (MQ=255)                                                                                                                                                 gactaATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGcc  <  1:1004944/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCC  >  NC_000913/3440231‑3440525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑