Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,356,201	0	T	G	43.4%	144.2 / 18.6	76	T304P (ACC→CCC)	sapA	antimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (6/37);  new base G (33/0);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 9.26e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCC  >  NC_000913/1356054‑1356348                                                                                                                                                      |                                                                                                                                                       cGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGTGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑Ggtg                                                                                                                                                       <  2:701688/149‑1 (MQ=255)  gTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑Ggggt                                                                                                                                                      >  1:641932/1‑149 (MQ=255)     gTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAAt                                                                                                                                                 <  1:789981/151‑1 (MQ=255)      tCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAAt                                                                                                                                                 <  1:129547/150‑1 (MQ=255)       cATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGc                                                                                                                                               <  2:1175877/151‑1 (MQ=255)            gCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGAt                                                                                                                                          <  2:1068759/151‑1 (MQ=255)              ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTTAAAGcc                                                                                                                                              >  1:1026625/1‑145 (MQ=255)                aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGc                                                                                                                                      <  1:1076585/151‑1 (MQ=255)                aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGc                                                                                                                                      <  2:1090526/151‑1 (MQ=255)                aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGc                                                                                                                                      <  1:708843/151‑1 (MQ=255)                 cGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGGTTAATGCCAGaaaaggg                                                                                                                                     >  2:287168/1‑145 (MQ=255)                  gAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTAAATGCCCGATAGGc                                                                                                                                      >  2:214098/1‑149 (MQ=255)                   aGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGa                                                                                                                                    <  2:440550/150‑1 (MQ=255)                   aGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAc                                                                                                                                   <  1:55268/151‑1 (MQ=255)                      cgcgcgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑Gtt                                                                                                                               <  1:311270/151‑1 (MQ=255)                        cgcgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCa                                                                                                                             <  2:990190/151‑1 (MQ=255)                          cgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATc                                                                                                                           <  1:213588/151‑1 (MQ=255)                              aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCa                                                                                                                        <  2:523137/150‑1 (MQ=255)                                 aTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGAc                                                                                                                    <  2:847854/151‑1 (MQ=255)                                    gagGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCa                                                                                                                 <  2:708099/151‑1 (MQ=255)                                      ggCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGc                                                                                                               <  1:744546/151‑1 (MQ=255)                                        ccgccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGTTAAAATGCAGAAAAGCGGC‑GTTCATCCCCCGACCCAGC‑g                                                                                                             >  2:125539/1‑150 (MQ=255)                                            cgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGGTTAAATG‑CAGAAAGGGGAC‑GGTTATccccaggagcagggctta                                                                                                          >  2:916225/1‑135 (MQ=255)                                             gTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAAc                                                                                                       <  1:658971/151‑1 (MQ=255)                                               ttCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGTTAAATGTCCGAAAAGCGGC‑GTTTACCCCAAGACCCAGC‑ggttaa                                                                                                        >  2:213881/1‑143 (MQ=255)                                               ttCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTAAATGCCAGAAAGGCGAC‑GTTCATCCCAGGGCGCAGC‑GTTaaa                                                                                                        >  1:159558/1‑148 (MQ=255)                                                     cGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAgcgc                                                                                               <  2:408495/151‑1 (MQ=255)                                                         ccATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTAAATTCCAGATAAGCGGCGGTCCTTCCCAGGAC‑CAGC‑GGTAAAAACAAACGCgggg                                                                                           >  2:836463/1‑150 (MQ=255)                                                          cATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTTAAAGCCAGATAAGCGAC‑GTTTATCCCAGGACGCCGC‑GGTAAACGCAAACGCggg                                                                                            >  1:728379/1‑149 (MQ=255)                                                          cATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGgtc                                                                                          <  2:331919/151‑1 (MQ=255)                                                          cATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑GTTGGC‑GGGGTTTAAAGGCAGAAAGGCGGC‑GTTCCTCCCCGGGCGGAGC‑GGTAAAAGCAAACGgggg                                                                                            >  1:125746/1‑149 (MQ=255)                                                          cATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑CTTTGG‑GGGGGTAAAAATCAAAAAAGCGGC‑GTTTATCCCAGGACCCAGC‑GTTTAACGccaacccg                                                                                              >  2:165701/1‑140 (MQ=255)                                                           ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGgtcg                                                                                         <  1:674919/151‑1 (MQ=255)                                                           ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTTAAAGCCAGATAGGGGGC‑GTTCATCCCAGGACGCAGC‑GTTAAAAGCAAGCGCggg                                                                                            >  1:834928/1‑148 (MQ=255)                                                              ataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGGG‑GGGGTTAAA‑GCCAGATAGGGGAC‑GTTCATCCCCGGACGCCGC‑GTTAAACGGAAACGCGGggcgtc                                                                                       >  1:856972/1‑150 (MQ=255)                                                              ataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGGTAAATGCCAGATAAGCGGC‑GGTTATCCCCGGACGCAGC‑GGTAAAAGCCAACGCggg                                                                                            >  2:606131/1‑145 (MQ=255)                                                                 gATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTAAAATGCAGAAAGGGGAA‑GGTTAACCCAAGACGCAGC‑GGTAAAAGAAAACGCCGGGCGGCaag                                                                                    >  2:430435/1‑148 (MQ=255)                                                                      aTTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGGTTAAAT‑CCAGATAGGCGAC‑GGTCATCCCCAGACGCCGC‑GTTTAAAGCAAACGCGGGGCGGCACGCcaaa                                                                               >  1:96905/1‑150 (MQ=255)                                                                      aTTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTAAAAGCCAGATAGGCGAC‑GGTCATCCCCGGACGCCGC‑GTTTAAAGCAAACGCGGGGCGGCACGCaaaa                                                                               >  1:286824/1‑150 (MQ=255)                                                                       ttGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTAAAAGCCAAATAGGCGAA‑GTTCATCCCCGGACGCAGCGGTTAAAGGCAA‑CGCGGGGTGTCACCCaaaa                                                                               >  1:514326/1‑149 (MQ=255)                                                                         gCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATgg                                                                            <  1:83996/150‑1 (MQ=255)                                                                              aGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTAAATGCCAGAAAAGCGAC‑GTTCATCCCCCGACGCAGC‑GGTAAAAACAAACGCGGGGCGGCCCCCAAAAAGGGTa                                                                         >  2:1026290/1‑148 (MQ=255)                                                                               ggCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctg                                                                     <  2:989890/151‑1 (MQ=255)                                                                                  gcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTTAAATCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAACGCGGGGCGTCACGCAAAATGGGTAgctgg                                                                    >  1:534441/1‑149 (MQ=255)                                                                                  gcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctggct                                                                  <  2:625622/151‑1 (MQ=255)                                                                                     ggTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGGTTAAAGCCCGAAAGGCGAC‑GTTCATCCCAGGACGCCGC‑GTTAAAAGCAAGCGCGGGGCGGCACGCCAAATGGGAAGctggctgg                                                                >  2:465419/1‑150 (MQ=255)                                                                                     ggTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGctggctgg                                                                <  1:262844/150‑1 (MQ=255)                                                                                     ggTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCtggctggc                                                               <  2:239701/151‑1 (MQ=255)                                                                                      gTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGGTAAAAGCCAGATAAGCGAC‑GGTCATCCCAGGACGCAGC‑GGTAAAAGCAAACGCGGGGCGTCACGCAAAATTGGTAGctggctgg                                                                >  1:507636/1‑149 (MQ=255)                                                                                      gTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTAAATGCCAGAAAGGCGAC‑GTTCATCCCCGGACGCAGC‑GTTAAACGCAAGCGCGGGGCGTCACGCCAAAAGGATAGctggctgg                                                                >  1:739058/1‑149 (MQ=255)                                                                                       ttattaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGTTTAATTGCAGATAGGGGGC‑GTTCATCCCCAGACGCAGC‑GGTAAACGCAAGCGCGGGGGGTCCCGCCAAAAGGATAGctgggtgg                                                                >  1:611546/1‑148 (MQ=255)                                                                                              tCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGTGGTTAAAGCCAGATAGGCGGC‑GGTTCTCCCCCGACGCCGC‑GGTAAACGCAAACGCGGGGCGTCACGCCAAAATGATAACTGGCTGGCAG‑caggcc                                                      >  2:397742/1‑150 (MQ=255)                                                                                               cGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑caggcca                                                     <  1:804029/150‑1 (MQ=255)                                                                                                 ccAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTAAAAGCCCGATAAGCGAC‑GTTCATCCCCGGACGCCGC‑GTTAAAAGCAAGCGCGGGGCGTCCCGCAAAAAGGATAGCTGGCTGGCAG‑caggcccgg                                                   >  1:243019/1‑150 (MQ=255)                                                                                                  cAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTAAATGCCAGATAGGGGGC‑GGTTATCCCCGGACGCAGC‑GGTAAACGCAAGCGCGGGTCGGCACGCCAAATGGATAACTGGCTGGCAG‑caggcccgg                                                   >  1:782857/1‑149 (MQ=255)                                                                                                         aGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTTAATGCCAGATAAGCGAC‑GTTCATCCCCGGACGCAGC‑GTTTAACGCAAACGCGGGGCGTCACGCAAAATGGATAGCTGGCTGGCAG‑Ccggcccggcc                                                 >  1:967239/1‑144 (MQ=255)                                                                                                          gcgcATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTc                                         <  1:340826/151‑1 (MQ=255)                                                                                                          gcgcATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTc                                         <  2:1052380/151‑1 (MQ=255)                                                                                                          gcgcATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTc                                         <  2:1144505/151‑1 (MQ=255)                                                                                                          gcgcATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTc                                         <  1:231908/151‑1 (MQ=255)                                                                                                             cATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTTAAAGCCAGAAAGGCGGC‑GTTCATTCCCGGACGCCGC‑GGTAAACGCAAACGCGGGGCGTCACGCAAAATGGGTAACTGGCTGGGAG‑Caagcccggcc                                                 >  1:344693/1‑140 (MQ=255)                                                                                                             cATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGTTTAAAGCCAGATAGGCGAC‑GGTCATCCCCCGAAGCAGC‑GGTAAAAGCAAACGCGGGGCGTCCCGCAAAAAGGGTAGCTGGCTGGGAG‑GAGGGCCGGCCCGaaaggc                                         >  1:701609/1‑144 (MQ=255)                                                                                                             cATGGCGGACAGCGGGATTATTTAGCGGCGG‑GTTTGC‑GGGGTTTAATGGCAGATAGGCGGC‑GTTTATCCCCGGGAGCAGC‑GGTTAAAACCAGCGCGGGGCGTCCCCCAAAAAAGAGAGGTGGGCGGCAG‑CAGGCCCGGGCAGAACGACGc                                       >  2:570029/1‑150 (MQ=255)                                                                                                               tGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGGTAAATACCGGAAAAGCGGC‑CGTCAACCCAAGCAGCAAC‑GTTAAAAGCAAAGGCGGGGCGTTTCGCCAAAAGGATAACCTGTTGGCAG‑GAGGTCCGGCCCGGACGTc                                         >  1:70053/1‑146 (MQ=255)                                                                                                                  cGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGGTTAAAGCCAAATAAGCGGC‑GTTTATCCCCCGGCGCAGC‑GGTAAAAGCAAACGCGGGGCGTCACGCAAAAAGGAAAGCTGGGTGGGAG‑CAGGGCAGGGCCGGACATCGCATTtcc                                 >  2:674881/1‑151 (MQ=255)                                                                                                                  cGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAAAGACAAATTGGCGAC‑GGTCATTCCCGGAACCAAC‑GTTAAAACAAAGCCCGGGTGGCCAAGGCAAAAGGAAAGGGGGCTTGCAG‑GAGGCCAAGCCAGCACGTCGCCATccc                                 >  1:669589/1‑151 (MQ=255)                                                                                                                      cAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGt                             <  2:477032/151‑1 (MQ=255)                                                                                                                      cAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGt                             <  1:421847/151‑1 (MQ=255)                                                                                                                         cGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAg                          <  1:822949/151‑1 (MQ=255)                                                                                                                             attattTAGCGGCGG‑TTTGGC‑GGGGTTTAATTCCAAATAAGCGAC‑CTTTATTCCCCGCCGCAGG‑GGTAAAAGCAAACGCCGGGTGGCACCCCAAAAAGAAAGCTTGCCTGCGG‑GAGGCCACGCCAGGACCTCCCAATcccc                                >  2:548042/1‑141 (MQ=255)                                                                                                                                attTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTc                   <  2:848077/151‑1 (MQ=255)                                                                                                                                attTAGCGGCGG‑TTTGGC‑GGGGGTAAAAGGCAGATAGGGGAC‑GTTCATCCCAGGGCGCAGC‑GGTAAAAGCAAAAGGGGGGGGTGACGCAAAAAAGAAAGCCGGCGGGGAGCCAGGCAAGG‑CAGAACGGGGCCTTTCCCgggccgggggttt                   >  2:499193/1‑140 (MQ=255)                                                                                                                                       ggcgg‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGAc            >  2:281476/1‑151 (MQ=255)                                                                                                                                             tttGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCg       >  2:20647/1‑151 (MQ=255)                                                                                                                                              ttGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCg       <  2:834922/150‑1 (MQ=255)                                                                                                                                                ggC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTg    >  1:198191/1‑151 (MQ=255)                                                                                                                                                 gC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgc   <  1:402379/151‑1 (MQ=255)                                                                                                                                                  c‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgcc  <  1:942131/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGC‑GTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAG‑CAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCC  >  NC_000913/1356054‑1356348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑