Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,861	0	G	T	44.7%	104.8 / 40.3	76	P259Q (CCA→CAA)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base G (12/30);  new base T (34/0);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 9.47e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCA  >  NC_000913/2434713‑2435005                                                                                                                                                     |                                                                                                                                                  aaaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGa                                                                                                                                                 <  2:669470/151‑1 (MQ=255)  aaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGaa                                                                                                                                                <  1:388386/151‑1 (MQ=255)      gATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGa                                                                                                                                              >  1:216555/1‑149 (MQ=255)      gATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATc                                                                                                                                            <  2:1043052/151‑1 (MQ=255)       aTGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTgg                                                                                                                                                  >  1:697700/1‑144 (MQ=255)       aTGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCa                                                                                                                                           <  1:463033/151‑1 (MQ=255)        tGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGGTCa                                                                                                                                           >  2:911544/1‑150 (MQ=255)         gAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAt                                                                                                                                          <  1:416949/150‑1 (MQ=255)            gAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCg                                                                                                                                     <  1:975568/151‑1 (MQ=255)            gAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCg                                                                                                                                     <  1:579012/151‑1 (MQ=255)            gAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCg                                                                                                                                     <  1:551375/151‑1 (MQ=255)              aataaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCgg                                                                                                                                    >  2:500603/1‑150 (MQ=255)                taaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCGGTc                                                                                                                                  >  1:321181/1‑150 (MQ=255)                   atCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCCGTCAggg                                                                                                                              >  2:218351/1‑151 (MQ=255)                    tCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATA‑ACCGTCCggg                                                                                                                              >  2:1058420/1‑150 (MQ=255)                     cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATA‑CCGGGCAgggg                                                                                                                             >  1:387253/1‑149 (MQ=255)                      aGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCGGGCAggg                                                                                                                              >  2:925536/1‑148 (MQ=255)                         tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGTAATGATA‑CCCGCCAGGGtgcggc                                                                                                                        >  1:101944/1‑146 (MQ=255)                             tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCGGGCAGGGGAAaccacc                                                                                                                     >  2:786712/1‑150 (MQ=255)                              atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAAAGCATC‑ACCGCCAGGGTAATACAccc                                                                                                                    >  1:195658/1‑149 (MQ=255)                                ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACcgcg                                                                                                                  <  1:669793/150‑1 (MQ=255)                                      aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATA‑CCGGGCAGGGGAATACACCCCCAcc                                                                                                               >  1:877439/1‑147 (MQ=255)                                      aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAAATCATA‑CCCGTCAGGGAAAACCaaccccacc                                                                                                               >  1:484077/1‑139 (MQ=255)                                         ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATTATA‑ACCGGCCGGGGGATCCACCCCGACCa                                                                                                              >  2:928584/1‑145 (MQ=255)                                             gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑ACGGTCCGGGGAATCCACCGCGACCAGATACAAccc                                                                                                    >  1:989904/1‑151 (MQ=255)                                             gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGccc                                                                                                    <  1:1088114/151‑1 (MQ=255)                                              tACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAACATA‑CCGGGCAGGGGAAACCACCCCGACCAGATAAAAccc                                                                                                    >  1:1070613/1‑150 (MQ=255)                                                          ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTg                                                                                         <  1:1072629/149‑1 (MQ=255)                                                            cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCCTA‑CCGGGCAGGGGAATCCACCGCGCCCAGATACAGCCCTTCCGCTTTTGcc                                                                                       >  1:262949/1‑149 (MQ=255)                                                            cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGGAATCCACCGCGACCAGATACAACCCTTCCGCTTTTTcc                                                                                       >  1:209932/1‑149 (MQ=255)                                                             aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGtt                                                                                    <  1:137280/151‑1 (MQ=255)                                                              ggCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtg                                                                                   <  1:219096/151‑1 (MQ=255)                                                               gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgc                                                                                  <  2:1047362/151‑1 (MQ=255)                                                                  gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgct                                                                                 <  1:128393/149‑1 (MQ=255)                                                                       tACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGGCAGGGGAAACCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGGTGCTtcccccc                                                                          >  2:107522/1‑150 (MQ=255)                                                                          gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGGCAGGGGAATCCACCGCGACCCGATACAGCCCTTCCGCCTTTTCCGGTGCTgccgcc                                                                           >  1:398545/1‑147 (MQ=255)                                                                          gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCCGTCAGGGGAATACACCGCGGCCCGAAACAACCCCTCCGCTTTTTTCGTTGCTgccgcc                                                                           >  1:579402/1‑147 (MQ=255)                                                                             ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑ACGGGCAGGGGAAACCACCCCGACCAGAAACAGCCCTTCCGCTTTTTCCGGTGCTGCCGCCAGCggt                                                                     >  1:1052757/1‑148 (MQ=255)                                                                             ggCTACAGCCTGAAATGTTCCGAACATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑ACGGGCAGGGGAATCCACCGCGACCAGATACAACCCTTCCCCTTTTTCCGTTGCTgccccc                                                                           >  1:783795/1‑144 (MQ=255)                                                                                  cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGGCAGGGGAATTCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCCTTGCTGCCGCCAGCGTTCTGTcc                                                               >  1:569620/1‑151 (MQ=255)                                                                                  cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTcc                                                               <  1:501845/151‑1 (MQ=255)                                                                                   aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCt                                                              <  1:500608/151‑1 (MQ=255)                                                                                    gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCtt                                                             <  2:580994/151‑1 (MQ=255)                                                                                        gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGGCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCCTTTGTCGTTTCTGCCGCCCGCGTTTTGTCCctt                                                            >  2:61844/1‑148 (MQ=255)                                                                                         aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCGGGCAGGGGAATCCACCCCGACCCGATACAGCCCTTCCGCCTTTTCCGTTGCTGCCGCCCGCGGTCTTTCCTTTtcc                                                         >  2:1067511/1‑150 (MQ=255)                                                                                             gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAg                                                    <  1:150433/151‑1 (MQ=255)                                                                                                 cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGTCAGGGGAATCCACCGCGGCCAGATACAGCCCCTCCGCTTTTTCCGGTGGTGCCGCCAGCGGTCTGTTCTTTGCCGCCCGCaa                                                 >  2:261229/1‑150 (MQ=255)                                                                                                   aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCCGGCAGGGGAATCCACCGCGACCAGATACAACCCTTCCGCTTTTTCCGTTTCTGCCGCCAGCGGTCTTTCCTTTTCCGCCAGCaa                                                 >  1:966566/1‑148 (MQ=255)                                                                                                    gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCCGTCAGGGGAAACCACCCCGGCCCGATACAACCCTTCCGCCTTTTCCGTTGCTTCCGCCCGCGGTCTGTCCTTTTCCGCCAGCaa                                                 >  1:422000/1‑147 (MQ=255)                                                                                                    gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActct                                             <  2:195657/151‑1 (MQ=255)                                                                                                     cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCCGGCAGGGGAATCCACCCCGACCAGATAAAGCCCCTCCCCTTTTTCCCTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCCActc                                              >  1:1076379/1‑149 (MQ=255)                                                                                                         attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGTCCGGGGAATCCACCCCGACCAGATAAAGCCCCTTCCCTTTTTCCGTTTCTTCCCCCCGCGGTCTTTCCTTTTCCGCCCGCaa                                                 >  2:730262/1‑142 (MQ=255)                                                                                                           tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAGCATACCCGGGCGGGGGATCCCACC‑CGACCAGATACAGCCCTTCCTCTTTTGTCGGTGCTGGCGCCAGCGTTCTGTCCTTTTCCGCCCCCCACCCTGCTATcc                                      >  2:459739/1‑151 (MQ=255)                                                                                                           tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGGCAGGGGAATCCCCCGCGACCAGATACAGCCCTTCCCCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATcc                                      >  1:117950/1‑151 (MQ=255)                                                                                                            ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCCGGCAGGGGAATCCACCGCGGCCAGATACAGCCCTTTCCCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATcc                                      >  1:211653/1‑150 (MQ=255)                                                                                                            ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATA‑CCGGTCAGGGTAATCCACCCCGACCAGATACAGCCCTTCCCCTTTTTCCGTTTCTGCCCCCAGCGTTCTGTCCTTTGCCGCCCGCAACCCTGCTATcc                                      >  1:1017887/1‑150 (MQ=255)                                                                                                              cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATcc                                      <  2:1065785/148‑1 (MQ=255)                                                                                                               gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGct                                  <  1:837598/151‑1 (MQ=255)                                                                                                                 tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCCGGCAGGGGAAACCACCCCCACCCGAAACAGCCCCTCCCCTTTTTCCGGTGCTTCCGCCCGCGGTCTTTCCCTTTCCGCCCGCAACTCTGCTTTTCAGctc                                 >  2:92069/1‑150 (MQ=255)                                                                                                                  aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGGCCGGGTAATCCACCCCGGCCCGATACAGCCCCTCCCCCTTTTGCGGTGCTGCCCCCCGCCGTCTTTCCCTTTCCGCCAGCCAATCTGCTATTCAGc                                   >  2:1131033/1‑147 (MQ=255)                                                                                                                     ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCgg                            <  2:216554/151‑1 (MQ=255)                                                                                                                     ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg                             <  2:946914/150‑1 (MQ=255)                                                                                                                      cGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATA‑CCGGGCAGGGGAATCCACCGCGCCCAGATACAGCCCCTCCCCTTTTTCCCTTTCTTCCCCCCGCCTTCTGTCCTTTGCCGCCCGCCACTCTGCTATCCACCTCTcc                              >  2:149584/1‑148 (MQ=255)                                                                                                                      cGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGATCATA‑CCGGGCAGGGGAATCCACCGCGACCAGAGACAGCCCCTCCCCTTTTTCCGTTGCTGCCCCCAGCCTTCTGTCCCTTTCCGCCCACAAATCTTCTATCCACCcctc                               >  2:808885/1‑147 (MQ=255)                                                                                                                       gCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATA‑ACGGTCAGGGGAATCCACCCCGACCAGATACAGCCCCTCCGCTTTTTCCGTTTCTGCCGCCCCCGGTCTTTCCTTTTCCCCCAGCCACTCTTCTTTCCAGCTCTcc                              >  2:54010/1‑147 (MQ=255)                                                                                                                        ccAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTg                         <  1:503201/151‑1 (MQ=255)                                                                                                                          aGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCGCCTTTTCCGTTGCTGCCCCCAGCGTTCTGTCCTTTTCCGCCCACAACTCTTCTATCCCGCTCTcc                              >  2:216814/1‑144 (MQ=255)                                                                                                                           gAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGGCAGGGGAAACCACCCCGACCCGATACAGCCCTTCCGCTTTTTCCGGTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCCGCAACTCTGCTATCCAGCCCTCCCGCTgggt                      >  1:622329/1‑149 (MQ=255)                                                                                                                              aTAGCGGGCCCATTGGCGGTTTTTGAAGATCATA‑CCGGGCAGGGGAATCCCCCGCGAACAGATACAACCCTTCCCCCTTTTCCCTTGCTGCCCCCAGCGTTCTGTCCTTTTCCGCCAGCCAATCTCCTATTCAGCTCTCCCGCTGGgtgt                    >  1:644724/1‑150 (MQ=255)                                                                                                                               tAGCGGGCCCATTGGCGGTTTTTGGAGAATATA‑ACCGGCAGGGGAATCCACCGCGGCCAGATACAACCCCTCCGCTTTTTCCGGTGCTGCCGCCCGCGGTCTGTCCCTTTCCGCCCGCCACTCTGCTAATCCGCTCTCCGGCTGGGTGTgg                  >  2:418505/1‑151 (MQ=255)                                                                                                                               tAGCGGGCCCATTGGCGGTTTTTGAAGATCAAA‑CCCGGCAGGGTAATCCACCGCGACCCGATACAGCCCCTCCGCCTTTTCCGTTGCTGCCGCCAGCGCTCTTTCCTTTTCCGCCAGCAACTCCGCTATCCCGCTCTCCCGCTGGGTGTgg                  >  2:915292/1‑151 (MQ=255)                                                                                                                                aGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTggg                 <  2:759851/151‑1 (MQ=255)                                                                                                                                  cGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCa               <  2:375637/151‑1 (MQ=255)                                                                                                                                   gggCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAc              <  2:440065/151‑1 (MQ=255)                                                                                                                                       ccATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAc          <  2:426903/151‑1 (MQ=255)                                                                                                                                             gCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt    >  2:993311/1‑151 (MQ=255)                                                                                                                                               ggTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa  <  2:590859/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATA‑CCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCA  >  NC_000913/2434713‑2435005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑