Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,268	0	T	A	44.4%	31.3 / 17.5	36	L24F (TTA→TTT)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (8/12);  new base A (0/16);  total (8/28)
Fisher's exact test for biased strand distribution p-value = 4.59e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483121‑2483416                                                                                                                                                    |                                                                                                                                                     cGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAataaataa                                                                                                                                                    <  2:901188/150‑1 (MQ=255)  gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATaaaa                                                                                                                                                  <  1:689766/151‑1 (MQ=255)        ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt                                                                                                                                                <  2:112331/147‑1 (MQ=255)        ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTa                                                                                                                                               <  1:321400/148‑1 (MQ=255)          aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGc                                                                                                                                           <  2:248094/150‑1 (MQ=255)                aaCGACAGTGACACTCCCTGAGTCTTGTGAAAAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACAAATCATGTTTTCTAATTCCATTGCCCAAAAGAAAAAGGCCCCTGAAAACAAAAAAAAAAAAACTACCGCCAATaaa                                                                                                                                    <  2:994306/151‑1 (MQ=255)                aaCGACAGTGACACTCCCGGAGATTTGTGAAAAAATTGGTTCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTTTTCAAATCCCATTGCCAAATAGGCATAGGCACCTGAAACCGAAAAAAAAAAAACTACCGCCAATa                                                                                                                                      <  2:1029054/149‑1 (MQ=255)                          acacTGCCAGAGTCTTGTTAAGAAGTTGGATCTGCATCCCCGTTGACAGAGGGCTCATCTGTATAAATAAGATCTTAAAATCCTATTACCCAAAAGGCAGAGGACCACAAAAACGAAAAAAAAAAACCTACCGCCAATAAAGAAAAGTa                                                                                                                            <  2:23058/149‑1 (MQ=255)                           ccctcccggagctttGTGAAAAATTTGGTTCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCTAATCCAATTGACAAAAAGGCATAGGCACCAAAAAACCCAATAAATAAAACTACCGCCAATAAAGAAAAGt                                                                                                                             <  1:667524/134‑1 (MQ=255)                                cttGACACTTTAGCAAAATTTGGTTCTCAATCCCCCGTCACAAAGGCCACTTGTTTACAAAAAATTTCTTCTAATTCCATGACCCAATATGCATAAACCCAGGAAAGAACAAAAAAAAAAACTTCCGCCAATAAAGAAAAGTAttt                                                                                                                         <  2:1128717/144‑1 (MQ=255)                                ccTGAGACTTGTGCAGAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGCCCAATAGGCATAGGCCCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTttct                                                                                                                      <  1:587706/149‑1 (MQ=255)                                          gtgaaaaaaTTGGATCTGCATCCCCCGTGACATGGGCGTCATCTGTACAAATCATTTCTTCAAATCCCATTGCCCAAAAGGAAAAGGCCCCTGAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                           <  2:1080294/144‑1 (MQ=255)                                                    tGGTTCTACATTCCCCGGAACTTAGGCGTCATCTTTAATAATCAGTTCTTCTAATTCCATTGCCAAAGAGGCATGGCCACCTGAAAACTAAATAAATAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt                                                                                                 <  2:490408/150‑1 (MQ=255)                                                    tGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt                                                                                                 <  1:971853/150‑1 (MQ=255)                                                            cATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAAt                                                                                        >  2:836512/1‑151 (MQ=255)                                                                ccccGTGACATAGGCGTCATCTGTACTAATCATTTTTTCTAATTCCATTGCCAAAAAGGAAAAGGCACTTAAAACCGCAATAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                      <  2:100806/149‑1 (MQ=255)                                                                ccccGTGACATAGGCGTCATCTGTACTAATCATTTTTTCTAATTCCATTGACCAATAGGCAGAGGCACCTAAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGaa                                                                                             <  2:1024878/142‑1 (MQ=255)                                                                ccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGAATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                      <  1:428724/149‑1 (MQ=255)                                                                          tAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt                                                                           <  1:1172599/150‑1 (MQ=255)                                                                            ggCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtc                                                                        >  2:981043/1‑151 (MQ=255)                                                                            ggCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtc                                                                        >  1:1171008/1‑151 (MQ=255)                                                                                   tCTGTACTACTCATTTTTCCTATTACCTTTGACAAAAAGGCATGGGCTCCTAAAAGCGCAAAAAAAAAAAGTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctctc                                                                    <  1:929601/148‑1 (MQ=255)                                                                                        aCTATTCTTTTCTTCTAATTCCATTGCCCAATAGGCAAAGGCCCCTAAAACCAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                                              <  1:636198/149‑1 (MQ=255)                                                                                         cTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                           >  2:1087317/1‑151 (MQ=255)                                                                                         cTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                           >  1:416563/1‑151 (MQ=255)                                                                                           aaTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc                                                               <  1:813716/145‑1 (MQ=255)                                                                                                 gTCTTCTAATTCCATTGCCAAATAGGCATAGGCACCTGAAACCGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                      <  1:1111901/148‑1 (MQ=255)                                                                                                 gTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTGAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaa                                                    <  2:227710/150‑1 (MQ=255)                                                                                                    ttctAATTCCATTACCCAATAGACATAGGCCCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaaa                                                   <  2:1188935/148‑1 (MQ=255)                                                                                                      ctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAt                                              >  2:1066787/1‑151 (MQ=255)                                                                                                            ccATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                        >  1:700401/1‑151 (MQ=255)                                                                                                                  aCCAATAGGCATAGGCCCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                                   <  1:840551/150‑1 (MQ=255)                                                                                                                     aaTAGGCATAGGCCCCTGAAACCGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAag                               <  1:1058472/151‑1 (MQ=255)                                                                                                                                 caCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct                   >  2:873097/1‑151 (MQ=255)                                                                                                                                 caCCTGAAAACACAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCattcattc                    <  1:794151/150‑1 (MQ=255)                                                                                                                                   ccTAAAAACGCAATAAATAAACCTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattca                       <  1:1056/145‑1 (MQ=255)                                                                                                                                       aaaaCGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCata              <  2:410976/150‑1 (MQ=255)                                                                                                                                                  aataaaACTACCGCCAATAAACAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGCATGAAAAGAGCATTCATTCTTCATATCAAACACAGTg  >  2:393323/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483121‑2483416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑