Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,536,285 Δ1 bp coding (300/720 nt) ompR ← response regulator in two‑component regulatory system with EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,536,2850G.99.0% 488.0 / ‑1.7 102coding (300/720 nt)ompRresponse regulator in two‑component regulatory system with EnvZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (48/53);  minor base C (1/0);  total (49/53)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 102 total aligned reads displayed.

GTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCA  >  NC_000913/3536139‑3536424
                                                                                                                                                  |                                                                                                                                           
gTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAgc                                                                                                                                        >  1:302134/1‑151 (MQ=255)
  aCCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAgcgc                                                                                                                                      >  1:1027459/1‑151 (MQ=255)
  aCCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAgcg                                                                                                                                       <  1:1327437/150‑1 (MQ=255)
   ccGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGcc                                                                                                                                     >  2:886694/1‑151 (MQ=255)
   ccGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGcc                                                                                                                                     >  1:973405/1‑151 (MQ=255)
    cGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCa                                                                                                                                    <  2:1265723/151‑1 (MQ=255)
     ggggTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCa                                                                                                                                    <  2:1510590/148‑1 (MQ=255)
      aGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAAt                                                                                                                                  >  2:807756/1‑151 (MQ=255)
       gtttAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCa                                                                                                                                    <  1:1564033/146‑1 (MQ=255)
       ggTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAAtc                                                                                                                                 <  2:351878/151‑1 (MQ=255)
          tAAGTTGGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAAtctc                                                                                                                               <  1:226881/150‑1 (MQ=255)
           aattttGAACTAACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCa                                                                                                                             <  2:894673/148‑1 (MQ=255)
           aaGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTcc                                                                                                                              <  1:59199/150‑1 (MQ=255)
            aGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTcc                                                                                                                              >  2:1567294/1‑149 (MQ=255)
               ttGGACTTATCGAAAGCAATTACCGCCTCTTCCTCCGACGGTGCGCCTCGCAGTTCGTTCGCCTAACGACCCACCACCGCACGGATACGGGCCTGCAGTTCACGCGGGTTAAACGGCTTTGGAGTGTAGGCTCGGCGACAGTCTcc                                                                                                                              >  2:462336/1‑146 (MQ=255)
                  aactcccggAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTAc                                                                                                                      <  2:758483/143‑1 (MQ=255)
                  aaCTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCt                                                                                                                        <  2:372280/149‑1 (MQ=255)
                    cTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACg                                                                                                                     >  2:1114182/1‑150 (MQ=255)
                        ccaaaaGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACg                                                                                                                <  2:1027444/148‑1 (MQ=255)
                        ccGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGa                                                                                                                    <  2:1508001/147‑1 (MQ=255)
                         cGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACgg                                                                                                               >  2:109694/1‑151 (MQ=255)
                           aaggCAATTTCCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGTGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATa                                                                                                                  <  2:1353970/143‑1 (MQ=255)
                           aaaGAAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTc                                                                                                             <  1:502089/151‑1 (MQ=255)
                             aGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCa                                                                                                           <  1:532065/151‑1 (MQ=255)
                               cAATTACCGCCTCTTCCTGTGACGGCGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCAct                                                                                                         >  1:811545/1‑151 (MQ=255)
                                aaTTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCActt                                                                                                        >  1:1545504/1‑151 (MQ=255)
                                aaTTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCActt                                                                                                        >  2:1319074/1‑151 (MQ=255)
                                aaTTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCActt                                                                                                        >  2:1470424/1‑151 (MQ=255)
                                 atgaCCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCActtc                                                                                                       <  2:527991/148‑1 (MQ=255)
                                  ttACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCActtct                                                                                                      >  2:1216140/1‑151 (MQ=255)
                                    aCCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCActtctt                                                                                                     <  1:1153633/150‑1 (MQ=255)
                                    aCCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACttcttc                                                                                                    <  1:764540/151‑1 (MQ=255)
                                         ccctTCCTGCGACGGGGCGCCTGGCAGTTCGGTGGCCTGACGACGCAGCACCGCACGGATACGCGCCAGCAGTGCACGCGGGTTAAACGTTTTTGGCAAGTAGTCTCAGCGCCAATCTCGAGGCCTACGACACGGTCCACTTCTTCCCCtt                                                                                               >  2:1446635/3‑151 (MQ=255)
                                           ctTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTc                                                                                             >  1:1430159/1‑151 (MQ=255)
                                            ttCCTTTGGCGGTGGGCGTGGCTGTTCGTGCGACTGGCGACGCAGCGCCGAGCGGATACGGCCCAGCAGTTCACGCGGGTTAAACGGTCTTGGAATTGAGTCACAGCGACAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCtt                                                                                               <  2:1085107/148‑1 (MQ=255)
                                            ttCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCg                                                                                            >  2:1425625/1‑151 (MQ=255)
                                             tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCttt                                                                                              <  1:1007501/148‑1 (MQ=255)
                                             tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCg                                                                                            <  2:1102865/150‑1 (MQ=255)
                                             tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCg                                                                                            <  1:377752/150‑1 (MQ=255)
                                             tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGc                                                                                           >  2:1502324/1‑151 (MQ=255)
                                              ccTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGGCGAAGCCCCGCACGGTTACGGGCCAGAAGTTCACGCGGGTTAAACGGTTTTGAAATGTAGTCTCCGCGCCAGTCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGcc                                                                                          <  2:1405356/151‑1 (MQ=255)
                                               cTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCg                                                                                         >  1:1010714/1‑151 (MQ=255)
                                                tgtgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGt                                                                                        >  1:1239290/1‑151 (MQ=255)
                                                 gtgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGt                                                                                        <  1:1081009/150‑1 (MQ=255)
                                                      ggTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCAt                                                                                  <  1:1541276/151‑1 (MQ=255)
                                                           gcCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATg                                                                              >  2:74311/1‑150 (MQ=255)
                                                            ccTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGAt                                                                            <  1:431277/151‑1 (MQ=255)
                                                            ccTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGAt                                                                            >  2:107021/1‑151 (MQ=255)
                                                              tGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCg                                                                          >  1:673877/1‑151 (MQ=255)
                                                               ggCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCg                                                                          <  2:397115/150‑1 (MQ=255)
                                                                gCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGc                                                                        <  1:1114197/151‑1 (MQ=255)
                                                                gCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGc                                                                        <  1:1127254/151‑1 (MQ=255)
                                                                 cAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGc                                                                        >  1:1169539/1‑150 (MQ=255)
                                                                  aGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCAt                                                                      >  1:1315559/1‑151 (MQ=255)
                                                                   gttcgttcGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATc                                                                     <  1:637517/151‑1 (MQ=255)
                                                                     tcgttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCgg                                                                   >  1:582214/1‑151 (MQ=255)
                                                                     tcgttcgCCTGACCACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGTGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCgg                                                                   >  2:1076628/1‑151 (MQ=255)
                                                                      cgttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCggg                                                                  <  1:389385/151‑1 (MQ=255)
                                                                       gttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGt                                                                 >  2:566480/1‑151 (MQ=255)
                                                                         tcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTg                                                               >  2:1481181/1‑151 (MQ=255)
                                                                          cgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGc                                                              <  1:1486611/151‑1 (MQ=255)
                                                                                acgacgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGa                                                         >  2:423885/1‑150 (MQ=255)
                                                                                  gacgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACt                                                       >  1:366185/1‑150 (MQ=255)
                                                                                  gacgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACt                                                       >  2:1379087/1‑150 (MQ=255)
                                                                                  gacgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTa                                                      <  1:238706/151‑1 (MQ=255)
                                                                                    cgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACg                                                    <  2:32085/151‑1 (MQ=255)
                                                                                    cgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGAAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACg                                                    >  1:470006/1‑151 (MQ=255)
                                                                                      cagcaCCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGa                                                   >  1:843268/1‑150 (MQ=255)
                                                                                      cagcaCCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGa                                                   >  1:111126/1‑150 (MQ=255)
                                                                                            cGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTc                                            >  1:1201744/1‑151 (MQ=255)
                                                                                                  gATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCaaa                                      <  2:1511331/151‑1 (MQ=255)
                                                                                                  gATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCaa                                       >  1:1280024/1‑150 (MQ=255)
                                                                                                   aTACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAAt                                     >  1:1277618/1‑151 (MQ=255)
                                                                                                   aTACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAAt                                     <  2:1160726/151‑1 (MQ=255)
                                                                                                     aCGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCg                                   >  2:1133957/1‑151 (MQ=255)
                                                                                                     aCGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCg                                   <  2:308183/151‑1 (MQ=255)
                                                                                                       gggCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGAc                                 <  1:768115/151‑1 (MQ=255)
                                                                                                       gggCCACCAGATCATGCGGGCTACACGGATTTGGAATGTAGCCTCAGCGCCAATCTCCAGGTCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGCCATCAGGTTCCTCTGACTACGAAGACGTCGGCAACTCGAc                                 >  2:1483292/1‑151 (MQ=255)
                                                                                                         gCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACaa                               <  1:324550/151‑1 (MQ=255)
                                                                                                         gCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACaa                               <  2:671848/151‑1 (MQ=255)
                                                                                                          ccAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAg                              <  2:1139182/151‑1 (MQ=255)
                                                                                                          ccAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAg                              <  2:1180453/151‑1 (MQ=255)
                                                                                                            agcagTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGcc                            >  2:653266/1‑151 (MQ=255)
                                                                                                             gcagTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGcc                            >  1:1538659/1‑150 (MQ=255)
                                                                                                              cagTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCa                           <  2:1262599/150‑1 (MQ=255)
                                                                                                               agTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATc                         <  1:1493666/151‑1 (MQ=255)
                                                                                                               agTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATc                         >  1:1381985/1‑151 (MQ=255)
                                                                                                                    aCGCGGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCAc                    <  1:273344/151‑1 (MQ=255)
                                                                                                                      gcgGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCa                  <  1:603098/151‑1 (MQ=255)
                                                                                                                       cgGGTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAg                 <  1:886710/151‑1 (MQ=255)
                                                                                                                         ggTTAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGt               <  2:181053/151‑1 (MQ=255)
                                                                                                                           ttAAACGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTa              >  1:184583/1‑150 (MQ=255)
                                                                                                                             aaaCGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAAc            >  1:74461/1‑150 (MQ=255)
                                                                                                                              aaCGGTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACAt          <  2:1440911/151‑1 (MQ=255)
                                                                                                                                  gTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTaaa      <  2:1276115/151‑1 (MQ=255)
                                                                                                                                  gTTTTGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTaa       >  2:51928/1‑150 (MQ=255)
                                                                                                                                   ttttGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAAt     >  1:54525/1‑151 (MQ=255)
                                                                                                                                    tttGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATc    >  1:1333865/1‑151 (MQ=255)
                                                                                                                                     ttGGAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATcc   >  1:80502/1‑151 (MQ=255)
                                                                                                                                       ggAATGTAGTCTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCa  <  2:447845/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
GTACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCA  >  NC_000913/3536139‑3536424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: