Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC40.2% 172.0 / 20.9 93intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (47/8);  new base C (0/37);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 3.03e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001770‑2002066
                                                                                                                                                       |                                                                                                                                                     
gCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcc                                                                                                                                                    <  1:554534/151‑1 (MQ=255)
  ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                                  >  2:992582/1‑151 (MQ=255)
  ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                                  <  2:772795/151‑1 (MQ=255)
  ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                                  >  1:835369/1‑151 (MQ=255)
      taGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgccg                                                                                                                                              >  2:884192/1‑151 (MQ=255)
       agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCgg                                                                                                                                             <  1:1457565/151‑1 (MQ=255)
         agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTg                                                                                                                                           >  1:1386867/1‑151 (MQ=255)
             aaTTCACGATAAACAGCCCTGCTTTATATGATTTATGGGAATGATTATCCTTTTCTGCAGGTTTTTTAATCGGACGATTAGTGGGTAAAATGGGGGTTTATTTGGGGGTTAAAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGc                                                                                                                                         <  2:549481/149‑1 (MQ=255)
                 cACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGa                                                                                                                                    >  1:172310/1‑150 (MQ=255)
                   cGAAAAACACCCTTGTTTTATATGATTTACCGGCTTGTTTATCCTTTTCTGCGGGTTTTTTAATCGGACGATTAGTGGGTAAAATGGGGGTTTATTTGGGGTTTACAGGAAAATTCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGc                                                                                                                                 <  1:443359/151‑1 (MQ=255)
                       aaaCAGCCCTGCGTTATAGGAGTTATCGGCTTTTTTTTCCGTTTATGCAGTTTTTTTACTGGTTCTTTTAGTGGTAAAATTGTGGGGTTTTTTGGGTGTGACGGGAAATTTCCAGCCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCa                                                                                                                                <  2:952907/148‑1 (MQ=255)
                       aaaCACCCCTGCGTTATATGATTTATCGGCTTGATTATCCGTTTCTGCGGGGTTTTTAATCGAACGATTAGGGGGTAAAAGGAGGGGTTATTTGGGGGTTAAAGTAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCAc                                                                                                                               <  2:87245/149‑1 (MQ=255)
                           aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                         >  1:1201443/1‑151 (MQ=255)
                            gCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                                        >  1:1011225/1‑151 (MQ=255)
                              ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGAc                                                                                                                      >  1:821729/1‑151 (MQ=255)
                                tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa                                                                                                                     >  1:300498/1‑150 (MQ=255)
                                   gTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                 >  1:666014/1‑151 (MQ=255)
                                    ttATATGAGTTATCGGCATGTTTACCCGTTTCTGCGGGGTTTTTAATGGGACGATTGGTGGGTGAAATGAGGGTTTATTGGGGGGTTAAAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                 <  1:582971/150‑1 (MQ=255)
                                    ttATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                                >  1:411609/1‑151 (MQ=255)
                                      atatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                              >  1:1555619/1‑151 (MQ=255)
                                             ttATGGGCATGATTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGTGGGTAAAATGAGGGGTTTTTTGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                          <  1:1564750/148‑1 (MQ=255)
                                             ttATCGGCTTGATTATCCTTTTCTGCAGGTTTTTTAATCGGACGTTTAGTGGGTGAAAGGGGGGTTTATTGGGGGGTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                              <  2:156213/144‑1 (MQ=255)
                                              tATCGGATTGTTTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGTAAAATGGGGGTTTTTTGGGGGTTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                                       <  1:134192/150‑1 (MQ=255)
                                               atgggaaTGATTATCCGTTTCGGCAGGTTTTTTAATCGGAGGATTAGGGGGGAAAAGGGGGGTTTATTGGGGGTTTACGGATAAATTCCAGCAAAAAAAACACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                          <  2:78930/140‑1 (MQ=255)
                                                    cATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                                >  2:57812/1‑151 (MQ=255)
                                                        aTTATCTGTTTCTGCGGGGTTTTAAATCGGACGATTAGGGGGTAAAATGGGGGGTTATTGGGGGGTTAAAGGTAAATTCCAGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                             <  1:1239211/150‑1 (MQ=255)
                                                         ttccccGTTTTTGAGGGTTTTTTAGTCGGTGATTTAGTGGGTCAAATGAGGGTTCAGTGGGGGCTAACGGCTAATTTCCAGCAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                              <  1:509902/144‑1 (MQ=255)
                                                         ttATCCTTTTCGGCAGGTTTTTTAATCGGACGATTAGGGGGTAAAAGGGGGGTTTATTGGGGGTTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGa                                                                                               <  1:1130764/147‑1 (MQ=255)
                                                          tATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg                                                                                          >  1:755183/1‑151 (MQ=255)
                                                          tATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg                                                                                          >  2:715812/1‑151 (MQ=255)
                                                           aTCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                                         >  1:1393449/1‑151 (MQ=255)
                                                            tCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                        >  1:721190/1‑151 (MQ=255)
                                                              cGTTTCTGGAGGGTTTTTAAGCGGACGATTTGTGGGTGAATGGGGGGGTTTTTTGGGGTTTAAAGGTAAATACCAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                             <  2:635481/144‑1 (MQ=255)
                                                                tttCTGCAGGTTTTTTAATCGGACGATTAGTGGGTGAAAGGGGGGTTTTTTGGGGGGTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                                     <  2:647643/150‑1 (MQ=255)
                                                                     gCAGGTTTTTTAATCGGACGATTGGGGGGTGAAAGGAGGGGTTATTGGGGGGTTACAGTTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                               <  2:128214/151‑1 (MQ=255)
                                                                     gCAGGGTTTTAAATCGGACGATTGGGGGGTAAAATGAGGGTTTATTGGGGGGTTAAAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                                  <  1:534550/148‑1 (MQ=255)
                                                                     gCAGGCATTTTAATCGACCGATTCGAGTGCGATATGTCGAGTTGTTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGCGACAGGACGTTGCTGACAAATTGCGCTTTATGTTTCCGGATGCGGCGTTAACg                                                                                  >  2:319163/1‑148 (MQ=255)
                                                                       aGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                              >  1:1240483/1‑150 (MQ=255)
                                                                        ggttttttATTGGGACGATTGGGGGGTAAAATGAGGGTTTATTTGGGTTTTACAGAAAATTTCGAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                                 <  1:1257184/143‑1 (MQ=255)
                                                                        ggttttttAATCGGACGATTAGTGGGTGAAATGAGGGTTTATTTGGGGGTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                                  <  1:683654/142‑1 (MQ=255)
                                                                        gggTTTTTATCCGGATAATGAGTGGGGGAAAGGAGGGGTTATTTGGGGTTTACGGGAAAATCCAAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                                   <  1:294418/144‑1 (MQ=255)
                                                                        gggTTTTTAATCGGACGATTAGTGGGTGAAATGCGGGGTTCTTTGGGGGTTACAGGTAAATTACAGGCTGAAAAAAACCCCGCCTTTGGCGGGGAAGCACGTTTCTGACAAATTACGCTTCATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                            >  2:873117/1‑151 (MQ=255)
                                                                         ggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                           >  1:75801/1‑151 (MQ=255)
                                                                         ggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                           >  1:1104828/1‑151 (MQ=255)
                                                                           tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                         >  2:1416853/1‑151 (MQ=255)
                                                                           tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                         >  1:1099963/1‑151 (MQ=255)
                                                                            ttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                        >  1:904394/1‑151 (MQ=255)
                                                                               tAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  1:1538029/1‑151 (MQ=255)
                                                                               tAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  2:509679/1‑151 (MQ=255)
                                                                                aaTCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  1:312585/1‑150 (MQ=255)
                                                                                   cGGACGTTTATGGGGTAAAATGAGGGTTTTTTTGGGGGTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   <  2:673153/149‑1 (MQ=38)
                                                                                    ggACGATTGGGGGGTAAAATGGGGGTTTTTTGGGGGTTTACAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                                <  1:309434/151‑1 (MQ=37)
                                                                                    ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                 >  1:646219/1‑150 (MQ=255)
                                                                                     gACGATTAGTGGGAGAAACGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAACAACCCCGCCGGTGGCGGGGAAGCACGAAGCTGACAAATTGCGCTTTAAGTTGCCGGCGGCGGCGTACACACCCTATCTGGCCGACaaa                                                                >  2:109716/1‑150 (MQ=255)
                                                                                          ttggggggaaaaaTGGGGGGTTTTTTGGGGTTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                                              <  1:660530/137‑1 (MQ=25)
                                                                                          ttggggggTAAAAGGAGGGGTTATTGGGGGGTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                            <  2:985929/144‑1 (MQ=38)
                                                                                          ttAGTGGGTGAAAGGGGGGTTTATTTGGGGGTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                           <  1:1238797/150‑1 (MQ=38)
                                                                                             gTGGGAGAAATGGGGGGTTTTTTGGGGTTAACGGGAAAATGCGAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                           <  1:178973/147‑1 (MQ=25)
                                                                                              tGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                                       >  2:945406/1‑150 (MQ=255)
                                                                                               gggTGAAAGGGGGGTTTTTTTGGGGTTTACAGTTAAATTCCGGGAAAAAAAAATCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaaa                                                      <  2:1234583/150‑1 (MQ=37)
                                                                                               ggaaaaaaTGAGGGGTGAATGGGGGGTTAAATAAAACTCCCAGTAAAAAAAAAACACCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGGTGCGGCATAAACGCCTTATCCCGCCTACAAAAATGTGCaaa                                                      <  2:1204482/145‑1 (MQ=255)
                                                                                                      aTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                               >  1:801162/1‑150 (MQ=255)
                                                                                                      aTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                               >  2:677910/1‑150 (MQ=255)
                                                                                                        gggggtTTATTGGGGGTTTAAAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                              <  1:434839/147‑1 (MQ=37)
                                                                                                        gAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                            >  1:646871/1‑151 (MQ=255)
                                                                                                        gAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                            >  2:965496/1‑151 (MQ=255)
                                                                                                        gAGGGGTTATTGGGGGTTTACAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaaa                                             <  1:1513624/150‑1 (MQ=37)
                                                                                                            gggtttttGGGGGTTAACAGGTAAATTCAAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                         <  2:1201427/146‑1 (MQ=25)
                                                                                                             gTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                        >  1:1372126/1‑150 (MQ=255)
                                                                                                              ttATTTGGGGGTTAGAGGTAAATTCCAGAAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                              <  2:96459/143‑1 (MQ=255)
                                                                                                              ttATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                                      >  1:1539435/1‑151 (MQ=255)
                                                                                                               ttttttGGGGGTAAAGGTAAATTCCGGGGAAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                         <  2:858704/146‑1 (MQ=25)
                                                                                                               tATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     >  2:612940/1‑151 (MQ=255)
                                                                                                                aTTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                    >  2:106080/1‑151 (MQ=255)
                                                                                                                aTTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                    >  2:217827/1‑151 (MQ=255)
                                                                                                                 ttggggggTTAAAGTAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                    <  1:472559/147‑1 (MQ=37)
                                                                                                                    gggggTTAAAGATAAATTCAAGCCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     <  1:558641/146‑1 (MQ=37)
                                                                                                                     ggggTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACtt                               >  1:373187/1‑151 (MQ=255)
                                                                                                                      ggggataacggttAAATTCAAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCaa                                  <  1:1108713/137‑1 (MQ=25)
                                                                                                                      gggTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                              <  1:1214048/151‑1 (MQ=255)
                                                                                                                       ggTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                             <  1:367868/151‑1 (MQ=255)
                                                                                                                             aCAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc                         <  1:371481/151‑1 (MQ=255)
                                                                                                                              cAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                        >  2:4494/1‑151 (MQ=255)
                                                                                                                              acggataaTTCCCAGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc                         <  1:1304215/145‑1 (MQ=25)
                                                                                                                                   aaaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                    >  1:198176/1‑150 (MQ=255)
                                                                                                                                   aaaTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                   <  2:789152/151‑1 (MQ=255)
                                                                                                                                   aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCCACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                        <  1:1088386/146‑1 (MQ=255)
                                                                                                                                    aaTTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATaa                  <  1:205458/151‑1 (MQ=255)
                                                                                                                                    aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                   >  1:941893/1‑150 (MQ=255)
                                                                                                                                        ccAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc              >  1:839549/1‑151 (MQ=255)
                                                                                                                                          aGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc              >  2:692611/1‑149 (MQ=255)
                                                                                                                                          aGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca             <  2:1172899/150‑1 (MQ=255)
                                                                                                                                           ggaaaaaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca             <  1:438766/144‑1 (MQ=255)
                                                                                                                                               gAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                          >  2:5929/1‑132 (MQ=255)
                                                                                                                                               aaaaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt       <  1:1282711/150‑1 (MQ=255)
                                                                                                                                                 aaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc      >  1:1157872/1‑150 (MQ=255)
                                                                                                                                                 aaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc      >  2:33554/1‑150 (MQ=255)
                                                                                                                                                   aaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAgg   >  1:1087795/1‑151 (MQ=255)
                                                                                                                                                     aaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc  >  1:1283940/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001770‑2002066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: