Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,341	0	A	G	44.6%	120.8 / 38.2	92	G337G (GGA→GGG)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (9/42);  new base G (40/1);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 4.45e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTG  >  NC_000913/213196‑213488                                                                                                                                                   |                                                                                                                                                         ccTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAgtgt                                                                                                                                                     <  1:491614/151‑1 (MQ=255)               cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCC‑GGGGAGGGGAAA‑GC‑TTCATGCgg                                                                                                                                     >  2:1106284/1‑151 (MQ=255)                gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGG‑GGGGAGTGTGAA‑AC‑TTaaataggg                                                                                                                                    >  1:136778/1‑145 (MQ=255)                gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCggg                                                                                                                                    <  1:314095/151‑1 (MQ=255)                       cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGGACA‑GA‑TTTATGCgggggggg                                                                                                                               >  2:1526557/1‑147 (MQ=255)                       cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGTACA‑GC‑TTAATGCGGGGGGCg                                                                                                                               >  1:596668/1‑149 (MQ=255)                       cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGat                                                                                                                             <  1:297429/151‑1 (MQ=255)                       cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGat                                                                                                                             <  1:603457/151‑1 (MQ=255)                          gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGTGTACA‑GC‑TTAAAGCGGGGGGCGAT‑Att                                                                                                                         >  1:455556/1‑151 (MQ=255)                             tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGTACA‑GC‑CTAAATCGGGGGGCGGT‑Atttg                                                                                                                       >  1:169029/1‑148 (MQ=255)                             tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGGACA‑GA‑CTAAATCGGGGGGGGAT‑ATTTGc                                                                                                                      >  2:316550/1‑151 (MQ=255)                                aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGTACA‑GC‑TTAATTTGGGAGGGGaggtttgc                                                                                                                       >  2:217563/1‑141 (MQ=255)                                   ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTcc                                                                                                                 <  2:776979/150‑1 (MQ=255)                                    cGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGGACA‑GC‑TTAATTCGGGGGGCGGT‑ATTCGCCCTcccc                                                                                                               >  1:694281/1‑149 (MQ=255)                                    cGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCcgc                                                                                                               <  2:169029/151‑1 (MQ=255)                                     gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGAGTGCA‑GC‑TTAAAGCGGGGGGGGAT‑ATTCGcccccc                                                                                                                 >  1:649031/1‑148 (MQ=255)                                      aCGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGt                                                                                                             <  1:940685/151‑1 (MQ=255)                                        gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGTGTACA‑GG‑TTAAAGCGGGGGGGGGT‑ATTTGCCCTCCCCGTg                                                                                                            >  2:1495324/1‑150 (MQ=255)                                        gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGTACA‑GC‑CTAATGCGGGGGGCggaaattcccc                                                                                                                     >  2:1356213/1‑132 (MQ=255)                                        gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGTACA‑GC‑TTTATGCGGGGGGGGCT‑ATTCGCCCCCCGCCTGc                                                                                                           >  1:982052/1‑151 (MQ=255)                                          tATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGAAGGGGACA‑GC‑GTCAAGAGGGGGGCGAT‑ATTCCCCCCCcgcggg                                                                                                            >  2:426329/1‑146 (MQ=255)                                           aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCa                                                                                                          <  2:1222400/149‑1 (MQ=255)                                           aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAg                                                                                                         <  2:1265410/150‑1 (MQ=255)                                            tCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGGACA‑GC‑GTAATGCGGGGGGCGAT‑ATTTGCCCCCCGCGCGCa                                                                                                          >  2:1109138/1‑148 (MQ=255)                                               gTCGCAACTGTGGTGGATTAAATCCGTCCCCGGGCAAAGCGAAAACATTGTGCCGCGGCAGACGTGGCTTCAAACGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGat                                                                                                       <  2:764786/148‑2 (MQ=255)                                                 cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGc                                                                                                  <  1:1147481/151‑1 (MQ=255)                                                 cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGc                                                                                                  <  2:378733/151‑1 (MQ=255)                                                  gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGGACA‑AA‑CTAAAACGGGGGGCGGT‑ATTCGCCCCCCCCGTGCAGaaaga                                                                                                    >  2:1242871/1‑144 (MQ=255)                                                  gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCg                                                                                                 <  1:1051474/151‑1 (MQ=255)                                                   cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGTGTACA‑GC‑TTAAAGCGGGGGGGGAT‑ATTTGCCCCCCGCGGGCAGACGAAGCgg                                                                                                >  1:1145138/1‑151 (MQ=255)                                                        gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGTAAA‑GC‑TTAATGCGGGGGGGGAT‑ATTCGCCCCCCCCGGGCAGACGAAGCGGTCAg                                                                                            >  1:1513349/1‑150 (MQ=255)                                                        gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCa                                                                                             <  1:1133213/149‑1 (MQ=255)                                                        gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCa                                                                                             <  2:662811/149‑1 (MQ=255)                                                        gtggtgGATTAAATCCGTCAACGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc                                                                                           <  2:288793/151‑1 (MQ=255)                                                         tggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGG‑GGGGGGGGTAAA‑ACTTTAATG‑GGGGGGGGAA‑ATTTGCCCCCCGCGGGGCAAAGAAGCGGGCAggg                                                                                          >  2:949100/1‑149 (MQ=255)                                                          ggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGCAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGTTCAgcg                                                                                          <  2:602458/150‑1 (MQ=255)                                                           gtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGGACA‑GC‑TTAATGCGGGGGGGGAT‑ATTCGCCCCCCGCGGGCCGAAGAAGCgg                                                                                                >  1:244564/1‑143 (MQ=255)                                                             ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGTAAA‑GC‑TTAATGCGGGGGGCGGT‑ATTTGCCCCCCGCCTGCCGACGAAACGGGCAgcgggc‑g                                                                                     >  2:1287071/1‑151 (MQ=255)                                                             ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgcgtgc                                                                                       <  2:411823/150‑1 (MQ=255)                                                                  aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGTGTACA‑GC‑TTTATGCGGGGGGCGGT‑ATTTGCCCCCCGCGTGCCGGCGGAGCGGTCCGCGTGC‑Ggtt                                                                                  >  2:1380561/1‑149 (MQ=255)                                                                  aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGTACA‑GC‑TTAATGCGGGGGGCGGT‑ATTTGCCCCCCGCGGGCAGACGAAGCGGGCAGCGGGC‑Ggtt                                                                                  >  1:1073094/1‑149 (MQ=255)                                                                    aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCaaa                                                                              <  2:882029/151‑1 (MQ=255)                                                                      ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGTGTACA‑GC‑TTAATGCGGGGGGCGGT‑ATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGTGC‑GGTTCaaa                                                                              >  1:128466/1‑149 (MQ=255)                                                                      ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGTACA‑GC‑TTAATGCGGGGGGCGAT‑ATTCGCCCCCCCCGGGCAGGCGAAACGGGCAGCGTGC‑GGTTTaaa                                                                              >  1:1130664/1‑149 (MQ=255)                                                                       cGTCACCGGGCCAAGCGGAAACATTGTGCCGGGGCAGACGGGGCTTCAACCGCTGGGATTTCCCGCGGGGCT‑GGGGAGTGGTCAGGC‑TTAATGC‑GGAGGGGATAATTCG‑CCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAgcg                                                                           <  2:937062/151‑1 (MQ=255)                                                                        gTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑TGGGAAAGGATA‑GC‑CTTATGCGGGAGGCGAT‑ATTCGGCCTCCGCGGGGAGACGCAACGGGTAGCGTGC‑GGTTTAAAacg                                                                           >  2:678755/1‑150 (MQ=255)                                                                        gTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAgcgc                                                                          <  2:1296231/151‑1 (MQ=255)                                                                          cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCa                                                                        <  1:353631/151‑1 (MQ=255)                                                                             cGGGCAAAGCGAAAACTTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTTGCCCCCCGCGGGCAGACGAAACGGGCAGCGGGC‑GGTTTAAAACGCCA‑gg                                                                     >  1:1403508/1‑150 (MQ=255)                                                                             cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGTGTACA‑GC‑TTAATGCGGGGGGGGGT‑ATTTGCCCTCCCCGCGCCGACGAAGAGGGCAGCGGGCGGTTTCaaacgcccgg                                                                       >  2:1553219/1‑143 (MQ=255)                                                                             cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGGAGA‑GC‑CTAAAGCGGGAGGCGAT‑ATTTGCCCCCCGCGTGGAGACGAAGCGGGCAGCGTGC‑GGTTCAAAACGcc                                                                         >  1:1238835/1‑147 (MQ=255)                                                                             cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGTACA‑GC‑TTAATACGGGGGGGGAT‑ATTCGCCCCCCGCGTGCAGACGAAGCGGGCAGCGGGC‑GTTTCAAAACGcc                                                                         >  1:1417657/1‑147 (MQ=255)                                                                                 cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGGGGCGAT‑ATTTGCCCCCCGCGTGCAGACGAAGCGGTCAGCGGGG‑GTTTTAAAGCGCCA‑GGGTtg                                                                 >  1:340981/1‑150 (MQ=255)                                                                                  aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGTACA‑GC‑TTTATGCGGGGGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGGGG‑GGTTCAAAGCGCCA‑GGGtt                                                                  >  1:1481097/1‑148 (MQ=255)                                                                                  aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATtgct                                                               <  1:709677/151‑1 (MQ=255)                                                                                    aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGTGTACA‑GC‑TTAATGCGGGGGGCGAT‑ATTTGCCCTCCCCCTGCAGACGAAACGGTCAGCGGGG‑GTTTCCAAACGCCC‑GGAAtggt                                                               >  1:1223624/1‑147 (MQ=255)                                                                                    aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGTAAA‑GC‑TTAATGCGGGAGGGGAT‑ATTTCCCCCCCCCCCGCAGACGAAACGGGCAGCGGGC‑GGTTCTAAACGCCC‑GGATtgct                                                               >  2:110696/1‑149 (MQ=255)                                                                                     gCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGTACA‑GC‑TTAATGCGGGAGGCGGT‑ATTCGCCCTCCCCGTGCAGACGAAACGGGCAGCGGGC‑GGTTCAAAGCGCCC‑GGGTtgctg                                                              >  1:977048/1‑149 (MQ=255)                                                                                      cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAAGGGT‑CC‑GC‑CTTATGCGGGAGGGGAA‑TTTCGCCCCCCCCCGGCAGAAGTAACGGGGCGCGTGCGGTTTAAAACCGCCC‑gggtg                                                                  >  1:868377/1‑142 (MQ=255)                                                                                      cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCat                                                           <  2:1513606/151‑1 (MQ=255)                                                                                       gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCata                                                          <  1:865930/151‑1 (MQ=255)                                                                                        aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGGATA‑AC‑TTAATGCCGGGGGCGGT‑ATTCGCCCCCCGCGGGCAGACGAAACGGGCAGCGGGC‑GGTTCCAAACGCCACGGATT‑CTGCat                                                           >  2:441491/1‑149 (MQ=255)                                                                                          aaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCC‑GGGGAGGGTACA‑GC‑TTAATTCGGGGGGCGGT‑ATTTGCCCCCCGCGTGCAGACGAAGCGGGCAGCGGGG‑GGTTTAAAACGCCC‑GGGTTGGTGCcta                                                          >  2:238514/1‑148 (MQ=255)                                                                                           aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGt                                                      <  1:1532486/151‑1 (MQ=255)                                                                                             aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCg                                                    <  2:101644/151‑1 (MQ=255)                                                                                               tgtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCg                                                    <  2:1021148/149‑1 (MQ=255)                                                                                                gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGc                                                 <  1:891150/151‑1 (MQ=255)                                                                                                 tgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGTACA‑GC‑TTAATGCGGGGGGCGGT‑ATTTGCCCTCCGCGTGGAGACGAAGCGGGCAGCGTGG‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTTTCGGGCg                                                >  1:1554857/1‑151 (MQ=255)                                                                                                  gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGGGGACA‑GC‑TTAATGGGGGAGGCGAT‑ATTTGCCCTCCCCGTGCAGAAGAAGCGGTCAGCGGGC‑GGTTCAAAACGCCC‑GGATTGCTGCATATTGTCGGGCg                                                >  1:830880/1‑150 (MQ=255)                                                                                                   ccGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTa                                              <  2:1403288/151‑1 (MQ=255)                                                                                                    cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGTACA‑AG‑TTAATGCGGGGGGGGGT‑ATTCGCCCTCCCCCGGGAGATGGAAGGGTTCGGGTGC‑GTTTTTAAACGCCC‑GGATTTCTCGCTATTGTCGGGCg                                                >  1:1045222/1‑148 (MQ=255)                                                                                                      tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGTACA‑GC‑TTAATGCGGGGGGCGAT‑ATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGG‑GGTTCAAAACGCCA‑GGGTTGCTGCATATTGTCGGGCGGaa                                             >  1:1558535/1‑149 (MQ=255)                                                                                                        gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGGAGTGTACA‑GC‑CTAATGCGGGGGGGGGT‑ATTCGCCCTCCGCGTGCCGACGCAGCGGGCAGCGGGC‑GGTTCAAAGCGCCA‑GGGATGCTGGATATTGTTGGGGGGAAcgg                                          >  1:1160448/1‑150 (MQ=255)                                                                                                        gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAAcggc                                         <  2:136778/151‑1 (MQ=255)                                                                                                        gCAGACGTGGCTTCAACCGCTGGAAGTCCCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATACTGTCGGGCTTAAcggc                                         <  2:108389/151‑1 (MQ=255)                                                                                                          aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGGACA‑GC‑TTAATACGGGGGGCGGT‑ATTCGCCCCCCCCGTGCAGACGAAACGGGCAGCGTGC‑GGTTCAAAGCGGCA‑GGGTTGCTGCATATTGTCGGGCGTAAaggcgg                                       >  1:455001/1‑151 (MQ=255)                                                                                                             cGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACg                                    <  1:693498/151‑1 (MQ=255)                                                                                                              gTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACg                                    <  2:921460/150‑1 (MQ=255)                                                                                                              gTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGt                                   <  1:1320189/151‑1 (MQ=255)                                                                                                                ggCTTCAACCGCTGGAATTACCGGCGGGGGT‑GGGGAGGGGACA‑GC‑TTAATGCGGGGGGCGGT‑ATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGC‑GGTTTCAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGGAAcggcgg                                       >  1:939885/1‑145 (MQ=255)                                                                                                                 gCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAg                                <  1:171338/151‑1 (MQ=255)                                                                                                                    tCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGc                               <  1:316554/149‑1 (MQ=255)                                                                                                                                aTTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTg                  <  2:911606/150‑1 (MQ=255)                                                                                                                                   aCCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCaa              <  2:627835/151‑1 (MQ=255)                                                                                                                                   aCCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCaa              <  2:591934/151‑1 (MQ=255)                                                                                                                                     cggcggGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAaga            <  2:635673/151‑1 (MQ=255)                                                                                                                                        cggGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt         >  2:1218345/1‑151 (MQ=255)                                                                                                                                        cggGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt         <  1:706740/151‑1 (MQ=255)                                                                                                                                         ggggCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTg        >  2:1554167/1‑151 (MQ=255)                                                                                                                                          gggCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg       >  1:68073/1‑151 (MQ=255)                                                                                                                                          gggCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg       <  1:242547/151‑1 (MQ=255)                                                                                                                                                gggAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTg  >  1:932946/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                         CCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCT‑GGGAAGTGTACA‑GC‑TTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCA‑GGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTG  >  NC_000913/213196‑213488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑