Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,748	0	C	A	40.7%	120.8 / 47.3	87	E55* (GAG→TAG)	nudF	ADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (39/12);  new base A (0/35);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 3.69e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGT  >  NC_000913/3177598‑3177894                                                                                                                                                       |                                                                                                                                                     cacTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTc                                                                                                                                                      >  2:86197/1‑151 (MQ=255)      tCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCAATGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑aaa                                                                                                                                                 >  1:1366990/1‑149 (MQ=255)       cACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑aaaaa                                                                                                                                               <  1:186788/150‑1 (MQ=255)         cccTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAAttt                                                                                                                                            >  2:263579/1‑151 (MQ=255)         cccTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAAttt                                                                                                                                            <  1:1123411/151‑1 (MQ=255)          ccTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTc                                                                                                                                           <  1:1155356/151‑1 (MQ=255)           ctctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTcc                                                                                                                                          >  2:698387/1‑151 (MQ=255)                cgatcatcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCccgcc                                                                                                                                      >  1:48665/3‑150 (MQ=255)                cAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCccgcc                                                                                                                                      <  2:767179/150‑1 (MQ=255)                 aaTCATCCCGGCAGCCATCTCCAGGACCCAGGGGGTTTCGCTGGGGTCGTACGGGGCAACCCGAACCAACTCAACCACCACAACTCCACCACGCACTGGGTCAAAGGGTAGCAAGCCTGCGGGGTGACCGCGTTA‑‑AAAAATTTCccgccg                                                                                                                                     <  2:1287163/150‑1 (MQ=255)                 aaTCACCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTCCGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCCC‑‑AAAATTTTCccgccg                                                                                                                                     <  2:166370/150‑1 (MQ=255)                    catcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCAcc                                                                                                                                 >  1:162145/1‑151 (MQ=255)                      tcCCGGCAACCATCTCCAGTAGCCAAGGGGGTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTc                                                                                                                               >  2:755976/1‑151 (MQ=255)                       cccGGCACCCATCTCCAGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCCCAATCAGCACAACTTCATCACGCACTGGGTAAAAGGGTAGCAAGACGGCGGCGTGACCGCGCTA‑‑AAAAATTCCCCGCCGCACCt                                                                                                                                <  2:873711/149‑1 (MQ=255)                           gCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGAc                                                                                                                          >  2:806889/1‑151 (MQ=255)                             aaCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCACTCAGCACAACTTCATCACGCACTGGGTCAAAGGGGAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTc                                                                                                                        >  1:861959/1‑151 (MQ=255)                             aaCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTc                                                                                                                        >  2:1320787/1‑151 (MQ=255)                               ccATCTCCAGTACCCAGGGGGTTTCGCGGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTCCACCACGCCCGGGGTAAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCAt                                                                                                                      <  1:691155/151‑1 (MQ=255)                                   ctcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGc                                                                                                                  >  2:1165343/1‑151 (MQ=255)                                      cAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                               >  1:810780/1‑151 (MQ=255)                                            ccAAGGGTTTTCGCTGGTGTCGTACGCGGCAACCCGATTCTGCTCAACCACCACACCTCCATCACGCACGGGGCCAAAGGGTAGCAAGCCTGCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGt                                                                                                              <  2:1107013/146‑1 (MQ=255)                                             cAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                         >  2:268165/1‑150 (MQ=255)                                             cAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                         >  2:1010557/1‑150 (MQ=255)                                                ggggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAACACAACTTCATCACCCCCTGGGTCAAAGGGTAGCAAGACTGGGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGa                                                                                                      <  1:303906/150‑1 (MQ=255)                                                 gggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                                                                                                    <  1:268168/151‑1 (MQ=255)                                                  ggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                                                                                                    >  1:360690/1‑150 (MQ=255)                                                     ttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                 >  2:779107/1‑150 (MQ=255)                                                     ttCGCTGGTGTCGTACGCGGCAATCCAAATCTGCTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                 <  1:1100242/150‑1 (MQ=255)                                                          tGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaa                                                                                            >  1:1415232/1‑150 (MQ=255)                                                             tgtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACTGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                         >  2:207214/1‑150 (MQ=255)                                                                 gTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                     >  1:722607/1‑150 (MQ=255)                                                                     gcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                    >  2:1233921/1‑147 (MQ=255)                                                                     gcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                    >  2:1282405/1‑147 (MQ=255)                                                                       ggCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGCCTGCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                                                                                <  1:624762/149‑1 (MQ=255)                                                                       gaaaaTCCAAATCTCCTCAATCACCACACCTTCTTCCCGCAGTGGGTCAAAGGGTGCCAAGACTGCGGCGTAACGGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                                                                              <  1:776762/148‑1 (MQ=255)                                                                        gaaaTCCGAATCGCCTCAATAAGCACAACTTCACCACGCACTGGGTAAAAGGGTAGCAACACGGCGGCGTGCCCGCGCAA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGa                                                                                 <  2:118493/145‑1 (MQ=255)                                                                          aaTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTatt                                                                           >  1:38185/1‑149 (MQ=255)                                                                          aaTCCGAATCTGCTCAACCAGCACAACTTCACCCCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAt                                                                           <  1:872580/151‑1 (MQ=255)                                                                           aTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                          >  1:620305/1‑151 (MQ=255)                                                                             cggAATCTGCTCAATCAGCTCAACTTCGCCACGCGTTGGGTCAAAGGGACACAACACCCCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                        <  1:1024861/149‑1 (MQ=255)                                                                             ccGAATCTTCTCAATCAGCCCAACTTCTCCACGCCCGGGGTCAAAGGGTAGAATGACTCCGGCGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAAAGACGATGACGAAATCTATAAAGATc                                                                               <  2:449396/144‑1 (MQ=255)                                                                             ccGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAGGGGTAGCAAGACTGCGGGGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                        <  2:1336082/151‑1 (MQ=255)                                                                             ccGAATCTGCTCAAACACCACACCTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACGGCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGa                                                                         <  2:103948/150‑1 (MQ=255)                                                                                aaTCTCCTCAATAAGCACAACTTCTCCACGCATTGGGTCAAAGGGTATCAATACTGCGGCGTACCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGa                                                                         <  2:736001/147‑1 (MQ=255)                                                                                      cTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                               >  1:1342228/1‑151 (MQ=255)                                                                                         aaTCAGCCCAACTTCATCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAGTGAAAAAAAGCCGCGa                                                            <  2:1010548/151‑1 (MQ=255)                                                                                         aaTCAGCACACCTTCACCACCCCTGGGGTAAAAGGTTAACAAGCCTGCGGCGTGGCCCCGCAA‑‑AAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                               <  2:1334530/148‑1 (MQ=255)                                                                                         aaTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                               <  1:1286831/148‑1 (MQ=255)                                                                                         aaTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                             >  2:1552371/1‑150 (MQ=255)                                                                                         aaTCAGCACAACTCCATCCGTTACTGGGTCAAGGGGTAACAAGCCTGC‑GTG‑GGCCGCGGTCAAAAAAATTTTCCGCCGCACCTCATGACGCAATTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaa                                                                       <  2:1008243/140‑1 (MQ=255)                                                                                         aaTCAGCACAACTCCACCACGCACTGGGTCAAAGGGTAGCAAGCCGGCGGCGTGCCCGCGCAA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                             <  1:415495/150‑1 (MQ=255)                                                                                          accaccaaaatttCACCACTCGCTGGTTCAAAGGGTAGAAAGACGGGGGCGGGACCGCGCAC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                             <  1:229360/138‑1 (MQ=255)                                                                                           tCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt                                                           >  1:1349122/1‑150 (MQ=255)                                                                                             aGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATaa                                                         >  1:652234/1‑150 (MQ=255)                                                                                                acacCTTCACCCCGCACTGGGTCAAAGGAGAGCAGGACGGCGCCGAGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                      <  1:798480/150‑1 (MQ=255)                                                                                                       caccacgcacgGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtctc                                               <  2:106362/147‑1 (MQ=255)                                                                                                        accaCGCACTGGGTCAAAGGGTAGCAAGACTGGGGCGTGACCGCGCAC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtc                                                 <  2:1184747/145‑1 (MQ=255)                                                                                                          cacgcacgGGGTAAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                           <  1:1035559/151‑1 (MQ=255)                                                                                                              cccggggcaaaaGGGTAGCAAATCGGCGGCGGGACCGCGACA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaa                                         <  1:1181372/140‑1 (MQ=255)                                                                                                              cACGGGGTCAAAGGGTAGAAAGACGGCGGCGTGACCGCGCAA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                       <  1:593427/151‑1 (MQ=255)                                                                                                               aCTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                       >  2:1509607/1‑150 (MQ=255)                                                                                                                 tGGGTCAAAGGGTAGCAAGACTGCGGCGAGACCGCGGTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAACCCGCGATAAAGTGTTTCTCGTGCAATAAtt                                    >  1:823879/1‑151 (MQ=255)                                                                                                                    gTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                 >  1:373318/1‑151 (MQ=255)                                                                                                                      aaaaGGGTACCAAGACTCCGGCGTGCCCGCCCTA‑‑AAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTAc                               <  2:985358/150‑1 (MQ=255)                                                                                                                       aaaGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACa                              <  1:1533065/151‑1 (MQ=255)                                                                                                                        aaGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt                             >  1:1144547/1‑151 (MQ=255)                                                                                                                         aGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                            >  2:490304/1‑151 (MQ=255)                                                                                                                         aGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                            >  1:595102/1‑151 (MQ=255)                                                                                                                          gggTAGCAAGACTGCGGCGTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCg                           <  1:1165359/151‑1 (MQ=255)                                                                                                                          gggAAGAAAGACTGGGGCGTGACCGGCCTG‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                            <  2:1557864/150‑1 (MQ=255)                                                                                                                              agaaaGACTGCGGCGTGCCCGCGCAA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttt                        <  2:810766/147‑1 (MQ=255)                                                                                                                                cAAGACGGCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttt                        <  1:1444602/148‑1 (MQ=255)                                                                                                                                 aaGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGc                    >  1:1041847/1‑151 (MQ=255)                                                                                                                                 aaGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGc                    >  1:121703/1‑151 (MQ=255)                                                                                                                                 aaGACGGCGGCGTGCCCGCGCAA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTg                     <  1:937684/150‑1 (MQ=255)                                                                                                                                  aGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGcc                   >  2:222343/1‑151 (MQ=255)                                                                                                                                   gACTGCGGCGTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCa                  <  1:779176/151‑1 (MQ=255)                                                                                                                                    aCTGCGGCGTGACCGCGCTC‑‑AAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCaa                 <  1:496168/151‑1 (MQ=255)                                                                                                                                     cTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCaa                 >  1:1324437/1‑150 (MQ=255)                                                                                                                                     cTGCGGCGTCCCCCCGCCA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCaa                 <  2:416463/150‑1 (MQ=255)                                                                                                                                         ggcgTGCCCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTa            <  2:182817/151‑1 (MQ=255)                                                                                                                                         ggcgTGACCGCGCAA‑‑AAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCaa                 <  2:479619/146‑1 (MQ=255)                                                                                                                                            gTGACCGCGCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAAc          <  1:283993/150‑1 (MQ=255)                                                                                                                                               aCCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc      >  1:392008/1‑151 (MQ=255)                                                                                                                                                 cgcgCTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAg    <  1:386455/151‑1 (MQ=255)                                                                                                                                                  gcgcTA‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAg    <  1:829495/150‑1 (MQ=255)                                                                                                                                                   cgcTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGt  >  2:474364/1‑151 (MQ=255)                                                                                                                                                   cgcTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGt  >  1:854530/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                     CACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTC‑‑AAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGT  >  NC_000913/3177598‑3177894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑