Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,944	T→A	G413G (GGT→GGA)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,944	0	T	A	100.0%	372.6 / NA	98	G413G (GGT→GGA)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (47/51);  total (47/51)

AACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGTGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTG  >  NC_000913/353796‑354091                                                                                                                                                     |                                                                                                                                                    aaCCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGgagt                                                                                                                                                   <  2:931182/151‑1 (MQ=255)   ccGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGc                                                                                                                                                 >  2:1312202/1‑151 (MQ=255)        ggACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGAt                                                                                                                                             <  1:613306/150‑1 (MQ=255)        ggACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATg                                                                                                                                            >  1:394435/1‑151 (MQ=255)            taaacGACCGCCAGTTGGTTGAGCAATACGCTGGATGTGCCGGTCATCGACTACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGTAAGCCCCGGAGTGCCTATg                                                                                                                                            <  2:96013/143‑1 (MQ=255)            gAGCCGGCCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATg                                                                                                                                        <  1:368099/151‑1 (MQ=255)                cGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATgg                                                                                                                                       >  1:841632/1‑148 (MQ=255)                cGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCt                                                                                                                                     >  2:1433967/1‑150 (MQ=255)                 gACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCtat                                                                                                                                   >  1:767740/1‑151 (MQ=255)                  aCCGCCAGTTAGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCtat                                                                                                                                   >  1:939168/1‑150 (MQ=255)                   ccGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATaa                                                                                                                                 <  1:1114837/151‑1 (MQ=255)                    cGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAAc                                                                                                                                >  2:1251055/1‑151 (MQ=255)                     gCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCta                                                                                                                                    <  2:360767/146‑1 (MQ=255)                     gCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACg                                                                                                                               >  1:1049481/1‑151 (MQ=255)                          ttGGGCGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGCTTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGt                                                                                                                              >  1:1069401/1‑147 (MQ=255)                           tGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAg                                                                                                                          >  1:95956/1‑150 (MQ=255)                             ggTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTg                                                                                                                       >  1:366140/1‑151 (MQ=255)                                gAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCt                                                                                                                     <  1:5719/150‑1 (MQ=255)                                    aaTACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAAt                                                                                                                 <  2:1306147/150‑1 (MQ=255)                                    aaTACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATg                                                                                                                >  1:1111743/1‑151 (MQ=255)                                     aTACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATg                                                                                                                >  1:1391448/1‑150 (MQ=255)                                     aTACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGa                                                                                                               <  1:1535737/151‑1 (MQ=255)                                       aCGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAg                                                                                                             <  2:795660/151‑1 (MQ=255)                                       aCGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGGATGGCTATAACGTGCAGTTGCTTAATg                                                                                                                >  2:1393624/1‑148 (MQ=255)                                         gCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTc                                                                                                           >  1:1073525/1‑151 (MQ=255)                                         gCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTc                                                                                                           <  1:88179/151‑1 (MQ=255)                                         gCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTc                                                                                                           <  1:1441098/151‑1 (MQ=255)                                         gCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTc                                                                                                           <  2:256787/151‑1 (MQ=255)                                             gATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCg                                                                                                       <  1:397625/151‑1 (MQ=255)                                              aTGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCgg                                                                                                      >  1:197395/1‑151 (MQ=255)                                                gtgCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCg                                                                                                    <  1:1407022/151‑1 (MQ=255)                                                  gCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCg                                                                                                    <  1:816997/149‑1 (MQ=255)                                                   ccGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAAc                                                                                                 >  1:795628/1‑151 (MQ=255)                                                   ccGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAAc                                                                                                 >  2:1158307/1‑151 (MQ=255)                                                    cGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAAcc                                                                                                <  2:1159669/151‑1 (MQ=255)                                                      gTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCgt                                                                                              <  2:601042/151‑1 (MQ=255)                                                        cATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCgtgt                                                                                            <  2:847945/151‑1 (MQ=255)                                                        cATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCgtgt                                                                                            >  1:932679/1‑151 (MQ=255)                                                        cATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCgtg                                                                                             >  2:22628/1‑150 (MQ=255)                                                         aTCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGtgtg                                                                                           >  2:1544186/1‑151 (MQ=255)                                                           cGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGc                                                                                         <  1:298990/151‑1 (MQ=255)                                                            gACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCg                                                                                        <  1:961854/151‑1 (MQ=255)                                                            gACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCg                                                                                        >  1:1214217/1‑151 (MQ=255)                                                                actactGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCaa                                                                                    <  1:1147034/151‑1 (MQ=255)                                                                actactGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCa                                                                                     >  1:141952/1‑150 (MQ=255)                                                                 ctactGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAAc                                                                                   >  2:992430/1‑151 (MQ=255)                                                                  tactGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACg                                                                                  >  1:1051138/1‑151 (MQ=255)                                                                   actGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAACGAAGTCACCGGCGAACCGTGTGGCGTCAAc                                                                                   <  2:391834/149‑1 (MQ=255)                                                                         aGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAgg                                                                           >  2:889498/1‑151 (MQ=255)                                                                             cGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATg                                                                       <  2:70582/151‑1 (MQ=255)                                                                              gAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATg                                                                       <  2:1041190/150‑1 (MQ=255)                                                                              gAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGc                                                                      >  1:1387446/1‑151 (MQ=255)                                                                               aaTCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGc                                                                      >  2:241541/1‑150 (MQ=255)                                                                                aTCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCtg                                                                    <  1:1302710/151‑1 (MQ=255)                                                                                 tCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCtgg                                                                   >  2:1127322/1‑151 (MQ=255)                                                                                  ccGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCtgg                                                                   >  2:289841/1‑150 (MQ=255)                                                                                          ccGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGa                                                            >  1:643157/1‑149 (MQ=255)                                                                                           cGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAgg                                                         <  2:1516467/151‑1 (MQ=255)                                                                                            gATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAggg                                                        <  1:1047829/151‑1 (MQ=255)                                                                                               tATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGcc                                                      <  1:96695/150‑1 (MQ=255)                                                                                               tATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGcc                                                      <  2:1170785/150‑1 (MQ=255)                                                                                                 tGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGTTGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCt                                                   >  1:25065/1‑151 (MQ=255)                                                                                                  ggCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCt                                                   <  1:682840/150‑1 (MQ=255)                                                                                                  ggCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCt                                                   <  2:1243069/150‑1 (MQ=255)                                                                                                  ggCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCt                                                   <  2:1460120/150‑1 (MQ=255)                                                                                                    cGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTgcc                                                <  2:539173/151‑1 (MQ=255)                                                                                                     gATTGCTCGCGGTCTGGACGACCGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGAGGTGGAAGGGCCGCTgccg                                               >  2:694867/1‑151 (MQ=255)                                                                                                         gCTCGCGGTCTGGACGACAGGCCGACGCTTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCTGCGAGAAAGGGATGCTGGTGGTGGAATGGCGGCTGCCGCCgg                                           <  2:214624/151‑1 (MQ=255)                                                                                                          cTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCggg                                          >  1:925759/1‑151 (MQ=255)                                                                                                           tCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCgggg                                         >  1:121718/1‑151 (MQ=255)                                                                                                              cgGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGgtgt                                      <  2:74364/151‑1 (MQ=255)                                                                                                                  cTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTAtt                                   >  2:605925/1‑150 (MQ=255)                                                                                                                    ggACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTACTGACGTCACCGGCGAACCGTGTGGCGTCAACGCGAAAGGGATGCTGGTGGTGGAAGGGCCGCGGCCGCCGGGgtgt                                      >  2:350628/1‑145 (MQ=255)                                                                                                                    ggACGACAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTc                                  >  2:1165504/1‑149 (MQ=255)                                                                                                                         acAGGCCGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCAt                           <  2:1141481/151‑1 (MQ=255)                                                                                                                              ccGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTggg                      <  1:915280/151‑1 (MQ=255)                                                                                                                               cGACGCGTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTgggg                     <  1:1048539/151‑1 (MQ=255)                                                                                                                                   gcgTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGTGTGTATTCAGACCATCTGGGGcgac                 >  1:513360/1‑151 (MQ=255)                                                                                                                                   gcgTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGcgac                 >  1:1239226/1‑151 (MQ=255)                                                                                                                                   gcgTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGcgac                 <  1:1314593/151‑1 (MQ=255)                                                                                                                                    cgTCTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGcgacg                <  2:1420510/151‑1 (MQ=255)                                                                                                                                       cTGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGacgacg             >  1:1389488/1‑151 (MQ=255)                                                                                                                                        tGGGAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCCCCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAGGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGacgacg             <  2:177469/150‑1 (MQ=255)                                                                                                                                         gggAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCgacgac              <  1:1023586/148‑1 (MQ=255)                                                                                                                                         gggAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCgacgac              <  2:1539849/148‑1 (MQ=255)                                                                                                                                         gggAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCgacgac              <  2:1528477/148‑1 (MQ=255)                                                                                                                                         gggAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCgacgac              <  1:1393648/148‑1 (MQ=255)                                                                                                                                         gggAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGc           <  1:992444/151‑1 (MQ=255)                                                                                                                                          ggAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGcc          <  1:69686/151‑1 (MQ=255)                                                                                                                                          ggAAGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGcc          <  1:765439/151‑1 (MQ=255)                                                                                                                                             aGCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCt       <  1:1475860/151‑1 (MQ=255)                                                                                                                                             aGCCCCGGAGCGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGCGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGc        >  2:602627/1‑150 (MQ=255)                                                                                                                                              gCCCCGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCtt      >  2:916410/1‑151 (MQ=255)                                                                                                                                                cccGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCttt     >  1:862221/1‑150 (MQ=255)                                                                                                                                                cccGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTtg    >  2:677830/1‑151 (MQ=255)                                                                                                                                                 ccGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTtgt   <  1:634386/151‑1 (MQ=255)                                                                                                                                                 ccGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGAGTGGCGTCAACGAGAAAGGGATGCTGGTGGAGGAAGGGACGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGTGATGATGGCCg         >  2:811863/1‑145 (MQ=255)                                                                                                                                                  cGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGTGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTtgtg  >  1:572233/1‑151 (MQ=255)                                                                                                                                                  cGGAGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTtgtg  <  1:1433992/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGTGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGTCACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTG  >  NC_000913/353796‑354091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑