Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,421,443 | 0 | A | C | 44.3% | 106.7 / 11.2 | 61 | Y40S (TAC→TCC) | folX | D‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase |
Reads supporting (aligned to +/- strand): ref base A (30/4); new base C (0/27); total (30/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.25e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAC > NC_000913/2421294‑2421592 | tCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAc > 1:385803/1‑151 (MQ=255) aTCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAccc > 1:696687/1‑151 (MQ=255) ccTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAcccc > 2:29104/1‑150 (MQ=255) ccTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccg < 2:816427/151‑1 (MQ=255) aGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgccg > 1:835473/1‑150 (MQ=255) aaacccTGAAAGGGCACACCCTGCCGTTATTTTTCAAAAAAAGACCCTTCT‑TTTGCGTCCTTTTATCGGAATAAAGAAAAAAAAAATAAACACCGGCAAGGATTTTTTTAAAAAGGTGACGACCCACTCCCCCGCCGATAAAGCGCGCAc < 2:401846/146‑1 (MQ=255) acccTGAAAGGCCACACCCTGCCGTTTTTATTCGTAAAAAAACCCTTCT‑TTTGCGTACTTTTATCGAAATAAAGAAAAAAAAAATAAACACCCGTAAGGATTTTTTTATCAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTa < 1:1062371/148‑1 (MQ=255) aaCCTGCAATGACACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACt > 1:443275/1‑149 (MQ=255) agtgacacaCCCTGCCTCTATTATGCGTATAAA‑AACCTTTGTTTTGCTTGCTTTTAGCGAAATAAAGAAAAAAAAAATAAACACCCGTGAGGTTATTTTTAGAAGTGTGCCGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGat < 1:1086538/146‑1 (MQ=255) aaggccacaCCCTCCCGTTATTATTCTTAAAAAGACCCTTCG‑TTTGCGTACGTTTAGCGAAATAAAGAAAAAAAAAATAAACACCCGACAGGATATTGTAATAAATGGGGCAATCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGat < 1:408661/146‑1 (MQ=255) aaTGCCACACCCTGCCGCTATTATTCGTAAAAAGAACCTTCT‑TTTGCGTACTTTTATCGAAATAAAGGAAGAAAAAATAAACACCCGTAAGGATATTGTTATCAATGTGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCg < 1:848045/146‑1 (MQ=255) aaTGACACACCCTCCCGTTATTATTCATATAAAGAACCTTCT‑TTTGCGTGCTTTTATCGAAATTAAGGAAGAAAAAATAAACACCCGTCAGAATTTTTTTATAATTTTGCCCATCCCCCACCCCGCCGATAAAGCGCGCACTAGCGa < 1:976938/147‑1 (MQ=255) ccctccctttttttttCGTAAAAAAACCCTTCT‑TTTGCGTTGTTTTAGCGAAATAAAGAAAAAAAAAATAAACACCCGTAAGGTTATTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGAt < 2:1092450/137‑1 (MQ=255) ccGTTATTATTCTTAAAAAGACCCTTCT‑TTTGCGTCCTTTTATGGAAATAAAGAAAAAAAAAATAACCACCCGTCAGGATATTTTTATAAATGGGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATgcgc < 1:203207/150‑1 (MQ=255) cGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTg > 1:457102/1‑151 (MQ=255) tattatTCTTAAAAAAACCCTTCT‑TTTGCGTACTTTTATCGAAATTAAGGAAAAAAAAATAAACACCCGTCAGGATTTTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGaa < 1:1231409/150‑1 (MQ=255) tattatTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAAt > 2:89173/1‑151 (MQ=255) attCGTATTAAGAACCTTCG‑TGTGCGTACGTTTATCGGAATTAAGAAAGAAGCAATTAACAACCGTAACGATATTGTTATCAATGGGAAGATCCAATACCCCCCCGATAAAGCACGAACTAGCGTAGATATCAACGATGCGCtaa > 2:1063483/1‑143 (MQ=255) ttaataaaaaGACCCTTTT‑TTTGCGTACTTTTATGGAAATAAAGAAAAAAAAAATTAACACCCGGCAGGATTTTGTAATAAATGTGACTACCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTAt < 2:360542/142‑1 (MQ=255) ttCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCg > 2:1029604/1‑151 (MQ=255) aaaaaGACCCTTCT‑TTTGCTAACTTTTATCGAAATAAAGGAAAAAAAATTAAACACCCGACAGGATATTTTTATCAATGTGACGACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCAcc < 1:1139064/148‑1 (MQ=255) aacaacctttCT‑TTTGCGTCTTTTTATCGGAATAAGGGAAAAAAAAATAAACCCCCGAAAGTATATTTTTATCAAAGGCGCCACCCCCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGt < 1:182826/142‑1 (MQ=255) aaaGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGt > 1:102342/1‑150 (MQ=255) aaaGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGt > 1:1118905/1‑150 (MQ=255) aaGACCCTTCG‑TTTGCGTATTTTTATGGAAATAAAGAAAAAAAAAATTAACACCCGTAAGGATATTGTTAAAAATGTGCCGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTaa < 1:873327/151‑1 (MQ=255) aGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAAc > 1:919050/1‑151 (MQ=255) cG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAAc > 2:41655/1‑150 (MQ=255) tttGCGTACTTTTATGGAAATAAAGAAAAAAAAAATAAACAACCGCCAGGATTTTGTTATGAATGGGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaa < 1:437533/145‑1 (MQ=255) tGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACattat > 2:521189/1‑151 (MQ=255) gCGTTCGTTTAGAGAAATAAAGAAAGAAAAAATAAACACCCGTCAGTATATTTTTAAAAATGTAACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACattat < 2:203126/150‑1 (MQ=255) tACTTTTATGGAAATAAAGAAAAAAAAAATAAACACCCGTCAGGATTTTTTTATAAATGGGACGTCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTc < 1:90662/149‑1 (MQ=255) aTCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGt > 1:151930/1‑149 (MQ=255) cGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTag > 2:1089769/1‑149 (MQ=255) ggAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTag > 2:329752/1‑148 (MQ=255) ggAATGAAGAAAAAAAAAATAAACACCCGACAGAATTTTTTTATCAGTGTGACAACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAgaga < 1:940929/151‑1 (MQ=255) aaTAAAGAAAAAAAAAATAAACACCGGAAAGAATTTTTTAATAAATGTGCCCACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGaa < 2:927471/150‑1 (MQ=255) aaTAAAGAAAAAAAAAATAAACACCCGTAAGTTTTTTTTTATAAATGGGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGaa < 2:640179/150‑1 (MQ=255) aaaaaaaaaTAAACACCCGAAAGGATTTTTTTAAAAATGTAACGACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATaa < 2:233470/139‑1 (MQ=255) aaaaaaaaTAAACACCCGACAGGATTTTGTTAAAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTtc < 1:102818/146‑1 (MQ=255) agaaATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTctct > 1:972339/1‑151 (MQ=255) aaaaTAACCACCCGTAAGGTTTTTTTAATAAATGTCACAACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCtt < 2:481402/150‑1 (MQ=255) aaTTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTg > 1:417270/1‑151 (MQ=255) aaTAAACACCCGTAAGGTTATTTTAATCAAGGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTg < 2:370222/151‑1 (MQ=255) tAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTg > 1:761088/1‑151 (MQ=255) aacaacCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTgg > 1:230763/1‑151 (MQ=255) acccGTAAGGATATTGTTATAAATGTGACGACCCCCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaa < 2:514444/148‑1 (MQ=255) aacCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaa > 2:640719/1‑150 (MQ=255) ccGTAAGGTTTTTTTTATCAATGTGCCGCTCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaaaa < 1:119543/150‑1 (MQ=255) cAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTa > 2:34075/1‑150 (MQ=255) ggATATTTTTATAAATGTACCGCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTa < 2:1192205/148‑1 (MQ=255) ttGTTATAAATGTAACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAgg < 1:1136701/150‑1 (MQ=255) tGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGAt > 2:1249930/1‑151 (MQ=255) attgtgctgATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCt < 2:1256774/141‑1 (MQ=255) tgtgACCACCCCCTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGa < 1:564384/150‑1 (MQ=255) tgagacgacCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGa < 1:1114100/147‑1 (MQ=255) aCGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCg > 1:966037/1‑151 (MQ=255) gATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGc > 1:151284/1‑150 (MQ=255) cACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGACTTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTg > 1:562332/1‑151 (MQ=255) cACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTg > 2:955144/1‑151 (MQ=255) cTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGa > 1:220144/1‑150 (MQ=255) tACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAc > 1:989601/1‑151 (MQ=255) aCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGt > 1:79778/1‑146 (MQ=255) | TCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCG‑TTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAC > NC_000913/2421294‑2421592 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |