Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,421,4430AC44.3% 106.7 / 11.2 61Y40S (TAC→TCC) folXD‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
Reads supporting (aligned to +/- strand):  ref base A (30/4);  new base C (0/27);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 2.25e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAC  >  NC_000913/2421294‑2421592
                                                                                                                                                      |                                                                                                                                                     
tCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAc                                                                                                                                                      >  1:385803/1‑151 (MQ=255)
  aTCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAccc                                                                                                                                                    >  1:696687/1‑151 (MQ=255)
    ccTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAcccc                                                                                                                                                   >  2:29104/1‑150 (MQ=255)
    ccTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccg                                                                                                                                                  <  2:816427/151‑1 (MQ=255)
        aGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgccg                                                                                                                                               >  1:835473/1‑150 (MQ=255)
                     aaacccTGAAAGGGCACACCCTGCCGTTATTTTTCAAAAAAAGACCCTTCTTTTGCGTCCTTTTATCGGAATAAAGAAAAAAAAAATAAACACCGGCAAGGATTTTTTTAAAAAGGTGACGACCCACTCCCCCGCCGATAAAGCGCGCAc                                                                                                                                  <  2:401846/146‑1 (MQ=255)
                       acccTGAAAGGCCACACCCTGCCGTTTTTATTCGTAAAAAAACCCTTCTTTTGCGTACTTTTATCGAAATAAAGAAAAAAAAAATAAACACCCGTAAGGATTTTTTTATCAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTa                                                                                                                                <  1:1062371/148‑1 (MQ=255)
                       aaCCTGCAATGACACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACt                                                                                                                                 >  1:443275/1‑149 (MQ=255)
                              agtgacacaCCCTGCCTCTATTATGCGTATAAAAACCTTTGTTTTGCTTGCTTTTAGCGAAATAAAGAAAAAAAAAATAAACACCCGTGAGGTTATTTTTAGAAGTGTGCCGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGat                                                                                                                        <  1:1086538/146‑1 (MQ=255)
                              aaggccacaCCCTCCCGTTATTATTCTTAAAAAGACCCTTCGTTTGCGTACGTTTAGCGAAATAAAGAAAAAAAAAATAAACACCCGACAGGATATTGTAATAAATGGGGCAATCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGat                                                                                                                        <  1:408661/146‑1 (MQ=255)
                              aaTGCCACACCCTGCCGCTATTATTCGTAAAAAGAACCTTCTTTTGCGTACTTTTATCGAAATAAAGGAAGAAAAAATAAACACCCGTAAGGATATTGTTATCAATGTGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCg                                                                                                                             <  1:848045/146‑1 (MQ=255)
                              aaTGACACACCCTCCCGTTATTATTCATATAAAGAACCTTCTTTTGCGTGCTTTTATCGAAATTAAGGAAGAAAAAATAAACACCCGTCAGAATTTTTTTATAATTTTGCCCATCCCCCACCCCGCCGATAAAGCGCGCACTAGCGa                                                                                                                            <  1:976938/147‑1 (MQ=255)
                                       ccctccctttttttttCGTAAAAAAACCCTTCTTTTGCGTTGTTTTAGCGAAATAAAGAAAAAAAAAATAAACACCCGTAAGGTTATTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGAt                                                                                                               <  2:1092450/137‑1 (MQ=255)
                                            ccGTTATTATTCTTAAAAAGACCCTTCTTTTGCGTCCTTTTATGGAAATAAAGAAAAAAAAAATAACCACCCGTCAGGATATTTTTATAAATGGGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATgcgc                                                                                                           <  1:203207/150‑1 (MQ=255)
                                             cGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTg                                                                                                         >  1:457102/1‑151 (MQ=255)
                                                tattatTCTTAAAAAAACCCTTCTTTTGCGTACTTTTATCGAAATTAAGGAAAAAAAAATAAACACCCGTCAGGATTTTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGaa                                                                                                       <  1:1231409/150‑1 (MQ=255)
                                                tattatTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAAt                                                                                                      >  2:89173/1‑151 (MQ=255)
                                                    attCGTATTAAGAACCTTCGTGTGCGTACGTTTATCGGAATTAAGAAAGAAGCAATTAACAACCGTAACGATATTGTTATCAATGGGAAGATCCAATACCCCCCCGATAAAGCACGAACTAGCGTAGATATCAACGATGCGCtaa                                                                                                        >  2:1063483/1‑143 (MQ=255)
                                                     ttaataaaaaGACCCTTTTTTTGCGTACTTTTATGGAAATAAAGAAAAAAAAAATTAACACCCGGCAGGATTTTGTAATAAATGTGACTACCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTAt                                                                                                   <  2:360542/142‑1 (MQ=255)
                                                     ttCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCg                                                                                                 >  2:1029604/1‑151 (MQ=255)
                                                          aaaaaGACCCTTCTTTTGCTAACTTTTATCGAAATAAAGGAAAAAAAATTAAACACCCGACAGGATATTTTTATCAATGTGACGACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCAcc                                                                                             <  1:1139064/148‑1 (MQ=255)
                                                            aacaacctttCTTTTGCGTCTTTTTATCGGAATAAGGGAAAAAAAAATAAACCCCCGAAAGTATATTTTTATCAAAGGCGCCACCCCCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGt                                                                                           <  1:182826/142‑1 (MQ=255)
                                                            aaaGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGt                                                                                           >  1:102342/1‑150 (MQ=255)
                                                            aaaGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGt                                                                                           >  1:1118905/1‑150 (MQ=255)
                                                             aaGACCCTTCGTTTGCGTATTTTTATGGAAATAAAGAAAAAAAAAATTAACACCCGTAAGGATATTGTTAAAAATGTGCCGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTaa                                                                                         <  1:873327/151‑1 (MQ=255)
                                                              aGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAAc                                                                                        >  1:919050/1‑151 (MQ=255)
                                                                      cGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAAc                                                                                 >  2:41655/1‑150 (MQ=255)
                                                                         tttGCGTACTTTTATGGAAATAAAGAAAAAAAAAATAAACAACCGCCAGGATTTTGTTATGAATGGGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaa                                                                                    <  1:437533/145‑1 (MQ=255)
                                                                           tGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACattat                                                                            >  2:521189/1‑151 (MQ=255)
                                                                            gCGTTCGTTTAGAGAAATAAAGAAAGAAAAAATAAACACCCGTCAGTATATTTTTAAAAATGTAACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACattat                                                                            <  2:203126/150‑1 (MQ=255)
                                                                               tACTTTTATGGAAATAAAGAAAAAAAAAATAAACACCCGTCAGGATTTTTTTATAAATGGGACGTCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTc                                                                          <  1:90662/149‑1 (MQ=255)
                                                                                      aTCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGt                                                                   >  1:151930/1‑149 (MQ=255)
                                                                                        cGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTag                                                                 >  2:1089769/1‑149 (MQ=255)
                                                                                         ggAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTag                                                                 >  2:329752/1‑148 (MQ=255)
                                                                                         ggAATGAAGAAAAAAAAAATAAACACCCGACAGAATTTTTTTATCAGTGTGACAACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAgaga                                                              <  1:940929/151‑1 (MQ=255)
                                                                                           aaTAAAGAAAAAAAAAATAAACACCGGAAAGAATTTTTTAATAAATGTGCCCACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGaa                                                             <  2:927471/150‑1 (MQ=255)
                                                                                           aaTAAAGAAAAAAAAAATAAACACCCGTAAGTTTTTTTTTATAAATGGGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGaa                                                             <  2:640179/150‑1 (MQ=255)
                                                                                                   aaaaaaaaaTAAACACCCGAAAGGATTTTTTTAAAAATGTAACGACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATaa                                                          <  2:233470/139‑1 (MQ=255)
                                                                                                    aaaaaaaaTAAACACCCGACAGGATTTTGTTAAAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTtc                                                   <  1:102818/146‑1 (MQ=255)
                                                                                                       agaaATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTctct                                                >  1:972339/1‑151 (MQ=255)
                                                                                                        aaaaTAACCACCCGTAAGGTTTTTTTAATAAATGTCACAACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCtt                                               <  2:481402/150‑1 (MQ=255)
                                                                                                          aaTTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTg                                             >  1:417270/1‑151 (MQ=255)
                                                                                                          aaTAAACACCCGTAAGGTTATTTTAATCAAGGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTg                                             <  2:370222/151‑1 (MQ=255)
                                                                                                             tAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTg                                          >  1:761088/1‑151 (MQ=255)
                                                                                                              aacaacCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTgg                                         >  1:230763/1‑151 (MQ=255)
                                                                                                                 acccGTAAGGATATTGTTATAAATGTGACGACCCCCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaa                                       <  2:514444/148‑1 (MQ=255)
                                                                                                                 aacCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaa                                       >  2:640719/1‑150 (MQ=255)
                                                                                                                   ccGTAAGGTTTTTTTTATCAATGTGCCGCTCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaaaa                                     <  1:119543/150‑1 (MQ=255)
                                                                                                                       cAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTa                                 >  2:34075/1‑150 (MQ=255)
                                                                                                                         ggATATTTTTATAAATGTACCGCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTa                                 <  2:1192205/148‑1 (MQ=255)
                                                                                                                              ttGTTATAAATGTAACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAgg                          <  1:1136701/150‑1 (MQ=255)
                                                                                                                               tGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGAt                        >  2:1249930/1‑151 (MQ=255)
                                                                                                                                      attgtgctgATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCt                   <  2:1256774/141‑1 (MQ=255)
                                                                                                                                        tgtgACCACCCCCTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGa                <  1:564384/150‑1 (MQ=255)
                                                                                                                                        tgagacgacCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGa                <  1:1114100/147‑1 (MQ=255)
                                                                                                                                            aCGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCg           >  1:966037/1‑151 (MQ=255)
                                                                                                                                              gATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGc          >  1:151284/1‑150 (MQ=255)
                                                                                                                                                  cACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGACTTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTg     >  1:562332/1‑151 (MQ=255)
                                                                                                                                                  cACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTg     >  2:955144/1‑151 (MQ=255)
                                                                                                                                                    cTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGa    >  1:220144/1‑150 (MQ=255)
                                                                                                                                                     tACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAc  >  1:989601/1‑151 (MQ=255)
                                                                                                                                                      aCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGt      >  1:79778/1‑146 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAC  >  NC_000913/2421294‑2421592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: