Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,212	T→A	E273V (GAA→GTA)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,212	0	T	A	100.0%	283.3 / NA	74	E273V (GAA→GTA)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (39/35);  total (39/35)

GTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGC  >  NC_000913/3440070‑3440357                                                                                                                                               |                                                                                                                                                  gTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTa                                                                                                                                           <  1:325240/151‑1 (MQ=255)   tttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTaa                                                                                                                                          <  1:633809/150‑1 (MQ=255)   tttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTaa                                                                                                                                          <  2:836897/150‑1 (MQ=255)        aGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGt                                                                                                                                    >  1:1096240/1‑151 (MQ=255)        aGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGt                                                                                                                                    >  1:986975/1‑151 (MQ=255)          gcgcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTa                                                                                                                                  <  1:588763/151‑1 (MQ=255)            gcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGc                                                                                                                                <  2:724933/151‑1 (MQ=255)             cATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCa                                                                                                                               <  2:401081/151‑1 (MQ=255)              aTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGc                                                                                                                                >  2:639320/1‑149 (MQ=255)                gCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCaga                                                                                                                             <  1:145025/150‑1 (MQ=255)                gCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCaga                                                                                                                             <  1:145720/150‑1 (MQ=255)                       gaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGAc                                                                                                                       >  1:982605/1‑149 (MQ=255)                       gaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAg                                                                                                                     <  1:236202/151‑1 (MQ=255)                       gaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAg                                                                                                                     <  2:854704/151‑1 (MQ=255)                         cAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTc                                                                                                                   >  2:1259639/1‑151 (MQ=255)                              cACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTc                                                                                                              >  1:431633/1‑151 (MQ=255)                                cGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                                                                                                             >  1:1108326/1‑150 (MQ=255)                                    aaGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATc                                                                                                        <  1:974741/151‑1 (MQ=255)                                      gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGt                                                                                                      >  1:828794/1‑151 (MQ=255)                                        cAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTc                                                                                                     >  2:571042/1‑150 (MQ=255)                                         aGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCa                                                                                                    >  2:319520/1‑150 (MQ=255)                                          gCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                  <  1:1125415/151‑1 (MQ=255)                                          gCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                  <  1:23075/151‑1 (MQ=255)                                           cACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                  >  1:1025380/1‑150 (MQ=255)                                           cACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                  >  1:857444/1‑150 (MQ=255)                                            aCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTGCGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACa                                                                                                 <  2:430309/150‑1 (MQ=255)                                            aCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                  >  1:1242693/1‑149 (MQ=255)                                               tCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgc                                                                                             >  1:304581/1‑151 (MQ=255)                                                  ttAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTGCCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgc                                                                                             <  2:852789/148‑1 (MQ=255)                                                  ttAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcg                                                                                            <  1:563186/149‑1 (MQ=255)                                                  ttAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgc                                                                                             <  1:1101469/148‑1 (MQ=255)                                                         cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgcagca                                                                                       <  1:814020/147‑1 (MQ=255)                                                         cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGcagcag                                                                                      <  1:548650/148‑1 (MQ=255)                                                         cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGcagcag                                                                                      <  2:81723/148‑1 (MQ=255)                                                                 gaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGgatcggatcg                                                                            >  2:672109/1‑150 (MQ=255)                                                                    tttttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCGGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAAc                                                                         <  2:659453/150‑1 (MQ=255)                                                                      tttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActct                                                                      >  2:92498/1‑151 (MQ=255)                                                                      tttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActct                                                                      >  2:1258810/1‑151 (MQ=255)                                                                      tttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActc                                                                       <  2:621097/150‑1 (MQ=255)                                                                       ttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTAAGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTg                                                                     >  2:1194684/1‑151 (MQ=255)                                                                           cAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTgg                                                                    <  2:367374/148‑1 (MQ=255)                                                                            aaGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtc                                                                <  2:370558/151‑1 (MQ=255)                                                                             aGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                               >  1:205787/1‑151 (MQ=255)                                                                             aGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                               <  1:672679/151‑1 (MQ=255)                                                                              ggTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtctc                                                              >  2:568526/1‑151 (MQ=255)                                                                                 tAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttc                                                           >  2:1040011/1‑151 (MQ=255)                                                                                  aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttct                                                          <  2:545328/151‑1 (MQ=255)                                                                                   ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTcttctt                                                         <  1:1097517/151‑1 (MQ=255)                                                                                     cGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTc                                                       <  2:1092438/151‑1 (MQ=255)                                                                                      gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTc                                                       >  2:15793/1‑150 (MQ=255)                                                                                         ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACtt                                                   >  1:1233064/1‑151 (MQ=255)                                                                                          tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACtt                                                   <  1:1168469/150‑1 (MQ=255)                                                                                          tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTc                                                  >  2:767351/1‑151 (MQ=255)                                                                                             ggAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAgg                                               >  2:1071663/1‑151 (MQ=255)                                                                                                gCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTg                                            <  2:408407/151‑1 (MQ=255)                                                                                                  tCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGAc                                          >  2:660749/1‑151 (MQ=255)                                                                                                   cAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACg                                         >  1:1115053/1‑151 (MQ=255)                                                                                                     aCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGt                                        >  2:438827/1‑150 (MQ=255)                                                                                                         cGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATc                                   >  1:1175118/1‑151 (MQ=255)                                                                                                         cGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATc                                   <  2:1106458/151‑1 (MQ=255)                                                                                                           gTACGCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCAc                                 <  2:474569/151‑1 (MQ=255)                                                                                                               gCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTa                              >  2:433159/1‑150 (MQ=255)                                                                                                               gCTGTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTa                              >  1:634374/1‑150 (MQ=255)                                                                                                                  gTACCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                          >  2:1151071/1‑151 (MQ=255)                                                                                                                    aCCAGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTTGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCaa                        <  2:264402/151‑1 (MQ=255)                                                                                                                       aGATCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACg                      >  1:564406/1‑150 (MQ=255)                                                                                                                         aTCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGaa                    >  2:375736/1‑150 (MQ=255)                                                                                                                         aTCACCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGaa                    >  2:265418/1‑150 (MQ=255)                                                                                                                            aCCGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCt                >  1:827633/1‑151 (MQ=255)                                                                                                                             ccGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCtt               >  1:1193701/1‑151 (MQ=255)                                                                                                                              cGATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTc              >  1:1262121/1‑151 (MQ=255)                                                                                                                               gATATAGTGGATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc             <  2:916201/151‑1 (MQ=255)                                                                                                                                       ggATAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttgtt      <  2:596315/150‑1 (MQ=255)                                                                                                                                          tAGCTACTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGc  >  1:531316/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                  GTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGC  >  NC_000913/3440070‑3440357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑