Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,900	0	A	G	40.8%	116.1 / 18.0	72	noncoding (72/88 nt)	serW	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (6/36);  new base G (29/0);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 2.37e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAAT  >  NC_000913/925753‑926050                                                                                                                                                      |                                                                                                                                                             gtgctcTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCTCTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑a                                                                                                                                                              <  1:1026329/143‑1 (MQ=255)   tCACTGCCGTACAGTTAGCTTAGAAATTCACAGGTAGCATACTCCGCGCGCCCGCCATGTTCCCTGCCGTACAGGCAGATAAAATGCGAAAAAAAAGCGCGCACTTTCGTACGAGCTCTTCTGTAAATATGTCGGTG‑AGGGGGGG‑ATTc                                                                                                                                                           <  1:1188455/149‑1 (MQ=255)   tCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCg                                                                                                                                                          <  2:981970/150‑1 (MQ=255)      cTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGG‑AGGGGGGG‑GGTCGaaac                                                                                                                                                      >  1:622214/1‑149 (MQ=255)        gCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGG‑AGGGGGGGAATTGGAAccc                                                                                                                                                     >  1:822583/1‑151 (MQ=255)         ccGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGG‑GTTCGAAccccc                                                                                                                                                   >  1:1152356/1‑151 (MQ=255)               aGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGGAccccc                                                                                                                                                   >  1:542016/1‑145 (MQ=255)                  cAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTg                                                                                                                                          <  1:631315/151‑1 (MQ=255)                    gCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGAACCCCCGGCACGTTg                                                                                                                                          >  2:675766/1‑149 (MQ=255)                          aaaTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATac                                                                                                                                 <  1:828398/151‑1 (MQ=255)                             ttCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑AGGGGGGG‑GTTTGAAACCCCCATACGGTTCCG‑tatata                                                                                                                                >  2:190034/1‑147 (MQ=25)                              tCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATacaca                                                                                                                              <  1:171424/150‑1 (MQ=255)                               cacaGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGGACCCCCGCTATGTTGGCG‑CATacaca                                                                                                                              >  2:466110/1‑149 (MQ=25)                               cacaGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATacacac                                                                                                                             <  2:1193215/150‑1 (MQ=255)                                  aGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTc                                                                                                                        <  1:90447/151‑1 (MQ=255)                                   ggTAACATACTCCACCCGCCCTCCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATAGGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTc                                                                                                                        <  1:860278/150‑1 (MQ=255)                                            cTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGAACCCCCCCTACGTTGCCG‑TATACACAC‑TTTCCAgg                                                                                                                    >  2:877471/1‑145 (MQ=255)                                              ccACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCt                                                                                                              <  2:62253/149‑1 (MQ=255)                                               cACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAAGGGGGGG‑AGTGGAACCACC‑CCACCAGGCCG‑TATACAAAC‑TTTCCAgg                                                                                                                    >  1:386288/1‑142 (MQ=21)                                                    gCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑TGGGGGGG‑GGTCGAAACCCCCATAAGTTGCCG‑GTTTCACAA‑CTTCCTGGCg                                                                                                                  >  2:95616/1‑139 (MQ=21)                                                     cccACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTTCAACCCCCCACACGCTTCCG‑TATACACAC‑CTTCCCGGCGTGCTCCCTccccc                                                                                                     >  2:658141/1‑147 (MQ=18)                                                      ccaccaTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑‑GTCGAACCCCCCCTACGTTTCCC‑GATACACAC‑TTTTCCGGCGGGCTCCTTccacc                                                                                                     >  1:393622/1‑145 (MQ=18)                                                       caccaTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACGC‑TTTCCAGGCGTGCTCCTTCAGCCAc                                                                                                   <  1:109318/151‑1 (MQ=255)                                                         ccaTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑c                                                                                                <  2:1267389/151‑1 (MQ=255)                                                            tGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GGTTGCAACCCCCCTACGTTGCCG‑TATACACAC‑TTTTCAGGCGGGGTCCTTCATcccccccg                                                                                               >  1:1262121/1‑144 (MQ=18)                                                            tGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑AGGGGGGG‑GGTTGGACCCCCGATACGTTGCCG‑TATAAACAC‑TTTCCCTGCGTGGTCCTTCCGcccccccg                                                                                               >  1:642336/1‑144 (MQ=18)                                                             gTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGac                                                                                            <  1:592751/151‑1 (MQ=255)                                                                cACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGacac                                                                                          <  2:570374/150‑1 (MQ=255)                                                                    gCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCAc                                                                                     <  2:1218377/151‑1 (MQ=255)                                                                       gTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGG‑GTTCGGACCCCCCGCACGTTGTCGCAATCCAC‑C‑CTTCCCCGCGGGCTCCTTCCGCCCCC‑CGGAGAccccccc                                                                                    >  1:702228/1‑144 (MQ=12)                                                                             acagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGtt                                                                            <  2:485295/151‑1 (MQ=255)                                                                             acagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGtt                                                                            <  2:773556/151‑1 (MQ=255)                                                                                gaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGAACCCCCCATACGTTTCCG‑GATACACAC‑CTTCCAGGCGTGGTCCTTCAGCCCCT‑CGGACACCCCCCCCAAAtttttt                                                                          >  2:18022/1‑150 (MQ=14)                                                                                        gCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCaaa                                                                <  1:260451/151‑1 (MQ=255)                                                                                            aaaaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGG‑GGGGGGGG‑GGTCGAAACCCCCGTACGTTTTCG‑TATATACAC‑TTTCCAGGGGGGCTCCCTCAGCCACC‑CGGGGACCCCACCAAAATATTTTTCTTACCAAAc                                                               >  1:767216/1‑149 (MQ=2)                                                                                              aaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTT‑GGGGGGGG‑GGTCGAACCCCCCGCACGCTGTCG‑CACAAAAAC‑TTTCCGTGGGTGCTGCTTTTGCCCCT‑CGGGGCCCTCACCCAAATGtttttggtcccaa                                                                 >  1:396046/1‑136 (MQ=2)                                                                                              aaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGG‑GTTTGAACCCCCCGTCCGGTGCCG‑CATTAACAC‑CTTCCCGGGGTGGTCCTTCCCCCACT‑CGGGCCCCCCCCCCAATttttttttctccaaacccc                                                             >  1:157230/1‑136 (MQ=2)                                                                                                   gCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTg                                                    <  1:818358/151‑1 (MQ=255)                                                                                                       gCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGAACCCCCCATAAGTTGCCG‑TATACACAC‑CTTCCCGGCGGGCTCCTTCAGCCCCT‑CCGACACCTCACCCAATTGTTTTGC‑TACCAAAACTCA‑TTGGGGGC‑a                                                >  2:191024/1‑150 (MQ=14)                                                                                                        cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTTGAAACCCCCATACGTTGCCG‑TATACACAC‑CTTCCCGGCGTGCCCCTTCAGCCACC‑CGGGCACCCCCCCAAAATTTTTTTC‑TACCAAAACTCC‑TTGGGGGC‑aa                                               >  2:1141461/1‑150 (MQ=11)                                                                                                         aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGAACCCCCCATACGCTGGCG‑CATAAACAC‑CTTCCAGGCGGGCTCCTTCTGCCACT‑CCGACAACCCACCAAAATGTTTTTT‑TACCAAAACCCA‑TGGGTGGC‑aa                                               >  2:182063/1‑149 (MQ=14)                                                                                                            ttCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGG‑GTTCGGACCCCCCATACGTTGGCG‑TATACAAAC‑TTTCCCGGCGTGCTCCCTCAGCCACC‑CGGACACCCCACCAAAATGTTTTTC‑TACCAAAACTCA‑TGGGGGGC‑AACcggg                                          >  2:293248/1‑151 (MQ=14)                                                                                                             tCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTTGGAACCCCCACAACTTGCCG‑TATACACAC‑CTTCCAGGCGGGCCCCTTCAGCCACT‑CGGACACCCCACCAAAATTTTTTTC‑TACCCAACCTCA‑TGGGTGGC‑AAAgggg                                          >  1:867923/1‑150 (MQ=14)                                                                                                              cGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGgcg                                        <  2:1228193/151‑1 (MQ=255)                                                                                                              cGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGgc                                         <  1:554476/150‑1 (MQ=255)                                                                                                               gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTTGAACCCCCCCTACGTTGCCG‑TATACACAC‑CTTCCAGGGGGGCTCCCTCAGCCACC‑CCGGCACCCCACCCAAATGTTTTTC‑TACCAAAACTCC‑CGGGGGGC‑AAAgggg                                          >  2:1007622/1‑148 (MQ=9)                                                                                                               gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGAACCCCCCAAACGGTGCCG‑TATAAACAC‑CTTTCAGGCGGGCTCCTTCAGCCCCC‑CGGACACCCCACCAAAATGTTTTTC‑TACCAAAACTCA‑TGGGGGGC‑AAAgggggg                                        >  2:247729/1‑148 (MQ=14)                                                                                                               gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GGTGCAACCCCCCCAACCGTGCCG‑CAAATACACACTTCCGGGCG‑GCACCCTCCCACACC‑CGGCCACCTCCCCCAATTTTTTTTT‑TACCCAACCCCA‑TTGGGGGC‑AAcccgg                                          >  1:143732/1‑144 (MQ=2)                                                                                                               gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GGTCGGAACCCCCCTACCTTTCCG‑TATAAACAC‑TTTTCCGGCGGGGGCCCTCCGCCACTCCGGCCCCCCCCCCAAAAT‑TTTTTC‑TACCCAACCCCC‑CGGGGGGC‑AACcggg                                          >  1:586862/1‑148 (MQ=2)                                                                                                                tACGAGCTCTTCTTTAAATATGGCGGTGTGGGGGGGG‑GTTGGAACCCCC‑ATAACTTTCCG‑TATACACAC‑TTTTCAGGGGGGCGCCCTCAGCCACT‑CGGGGCCCCCACCAAAATTTTTTTC‑TACCAAACCTCATTGGGGGGA‑AACggg                                           >  1:614801/1‑147 (MQ=9)                                                                                                                 aCGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑GTTCGCACCCCCCATACGTTGCCG‑TATACAAAC‑CTTTCAGGCGGGCTCCTTCCGCCCCT‑CCGGCACCCCCCCCAAATTTTTTTC‑TTCCCAACCTCA‑TTGGTGGG‑AACgggggg                                        >  2:391759/1‑146 (MQ=9)                                                                                                                  cGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGctac                                    <  2:95406/151‑1 (MQ=255)                                                                                                                   gAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGG‑GTTTGGACCCCCCCCACGTTTCCG‑TATAAAAAC‑TTTCCAGGCGGGCTCCCTCAGCCACT‑CCGACACCCCACCCAAATTTTTTGC‑TACCAAACCTCC‑TGGGGGGCAAACgggggccac                                     >  1:138500/1‑145 (MQ=9)                                                                                                                   gAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGctact                                   <  1:661027/151‑1 (MQ=255)                                                                                                                   gAGCTCTTCTTTAAATATGGCGGAG‑ATGGGGGG‑GTTCGGACCCCCCCTACCTTTTCG‑TATACACAC‑TTTCCAGGCGTGCTCCCTCACCCCCT‑CGGGCCCCACCCCCAAATTTTTTGC‑TTCCCAAACCCA‑TGGGGGGC‑AACGGGgcg                                        >  1:623220/1‑146 (MQ=9)                                                                                                                     gCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGGACCCCCCCTACGTTTCCG‑TATACACAC‑TTTTCAGGCGTGCTCCTTTCGCCACT‑CGGACACCCCACCAAAATGTTTTTC‑TACCAAACCTCA‑CTGGGGGG‑AACGGGGCGccac                                    >  1:382615/1‑148 (MQ=14)                                                                                                                        cttcttTAAATATGGCGGTG‑AGGGGGGG‑GTTCCAACCCCCCCTACGTTGTCC‑CATACACAC‑TTTCCCGGCGTGCTCCTTCAGCCCCT‑CGGACACCCCACCCAATTGTTTTTC‑TACCAAACCTCC‑TGGGTGGC‑AACGGGGGGctac                                    >  2:376583/1‑145 (MQ=17)                                                                                                                        cttcttTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACtata                                <  1:570210/149‑1 (MQ=255)                                                                                                                             ttAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGt                          <  2:632416/150‑1 (MQ=255)                                                                                                                              tAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGtt                         <  2:430307/150‑1 (MQ=255)                                                                                                                              tAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGt                          <  1:1173300/149‑1 (MQ=255)                                                                                                                                   atGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGt                    <  2:506566/150‑1 (MQ=255)                                                                                                                                   atGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTa                   <  1:601630/151‑1 (MQ=255)                                                                                                                                     ggCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAg                     <  1:997674/147‑1 (MQ=255)                                                                                                                                      gCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTaaaa                <  1:1041544/151‑1 (MQ=255)                                                                                                                                      gCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTaaa                 >  1:667762/1‑150 (MQ=255)                                                                                                                                           g‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTc           <  2:614414/151‑1 (MQ=255)                                                                                                                                           g‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTc           <  1:628083/151‑1 (MQ=255)                                                                                                                                             aGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCCCT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCa          <  1:51124/151‑1 (MQ=255)                                                                                                                                              ggggggg‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCa          >  2:150201/1‑150 (MQ=255)                                                                                                                                                ggggg‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaaga       <  2:48776/151‑1 (MQ=255)                                                                                                                                                   gg‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAagaaga    <  1:200601/151‑1 (MQ=255)                                                                                                                                                   gg‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAagaaga    <  1:513934/151‑1 (MQ=255)                                                                                                                                                      aTTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAAt  >  1:309107/1‑151 (MQ=255)                                                                                                                                                      aTTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAAt  <  1:1152736/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                             GTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGG‑ATTCGAACCCCCGATACGTTGCCG‑TATACACAC‑TTTCCAGGCGTGCTCCTTCAGCCACT‑CGGACACCTCACCAAATTGTTTTGC‑TACCAAACCTCA‑TGGGTGGC‑AACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAAT  >  NC_000913/925753‑926050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑