Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,596	0	G	A	41.1%	82.5 / 43.9	73	F13F (TTC→TTT)	ymgD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (34/9);  new base A (0/30);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 7.99e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222446‑1222745                                                                                                                                                        |                                                                                                                                                      ggCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCg                                                                                                                                                       >  2:595997/1‑151 (MQ=255)    aaCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGaa                                                                                                                                                     >  1:925006/1‑150 (MQ=255)       gCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAaaaca                                                                                                                                                 <  1:217709/151‑1 (MQ=255)       gCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAaaac                                                                                                                                                  >  2:9264/1‑150 (MQ=255)            cattacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAaaacaaac                                                                                                                                              >  2:227505/1‑149 (MQ=255)            cattacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAg                                                                                                                                            <  2:1118850/151‑1 (MQ=255)             attacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAgg                                                                                                                                           <  1:346694/151‑1 (MQ=255)              ttacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGc                                                                                                                                          >  1:276100/1‑151 (MQ=255)                 cccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGccc                                                                                                                                        >  1:343045/1‑150 (MQ=255)                  ccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGc                                                                                                                                      <  1:699144/151‑1 (MQ=255)                    attaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCa                                                                                                                                    <  2:1114900/151‑1 (MQ=255)                     ttaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGcc                                                                                                                                     >  2:725639/1‑149 (MQ=255)                         cGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTaa                                                                                                                                >  1:1139579/1‑150 (MQ=255)                            aacaaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGc                                                                                                                             >  1:994694/1‑150 (MQ=255)                            aacaaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGc                                                                                                                             >  2:699406/1‑150 (MQ=255)                               attttCTGCAACCTGTGCGCTGTCAGTTTTTGCCTTTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCGGCGCCCAGGTCATG‑GGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAAt                                                                                                                         <  2:245462/149‑1 (MQ=255)                               aaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAAt                                                                                                                         >  1:709014/1‑151 (MQ=255)                                aTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAAtt                                                                                                                        >  1:1163021/1‑151 (MQ=255)                                       cacccTGTGCGTTGTCTGTTTTTGCCTTTTCGTTGTTTTCTGCGAAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAATTTTGTAGTCCTGCGCCCAGTTTTTG‑GGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTtc                                                                                                                   <  1:45989/146‑1 (MQ=255)                                        aaCCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTtcatc                                                                                                                >  1:432465/1‑151 (MQ=255)                                             gtgCGTTGTCATTTTTTCCCTTTTGGTGGGTTTCTGCGCAAGTAATATAGGTTTCGGACGGCAAACCTTTTTTAATATTTTGGCCCTGCCCCCGGTTCTTT‑GGTGCGAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTtcatcatc                                                                                                             <  2:1013802/149‑1 (MQ=255)                                                 gcTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                                       >  2:694517/1‑151 (MQ=255)                                                   tcgcatttttcGCCATTTCGTTGTCTTCGGCGAAATTAATATGGGTTTCTGACGGCAAACTTTTTTAAATATTATCGTCCAGCGCCAAGTTTATT‑GGGGCAAAAACAAAAAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                                       <  1:29354/143‑1 (MQ=255)                                                   tGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGGAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCtt                                                                                                      >  2:766139/1‑150 (MQ=255)                                                    gTCATTTTTTGCCATTTCGTTGGCTTCTGCACAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGCCCTGCGCCCAGGTCTTT‑GGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                                       <  2:1218855/148‑1 (MQ=255)                                                     tCAGTTTTTGCCATTTCGTTGGTTTCTGCGAAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGTCCTGCCCCCAGTTCATG‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAt                                                                                                    <  2:656508/150‑1 (MQ=255)                                                     tCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGCTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTCCGCCCTGGTCATT‑GCCGCGAAAACAAACAGGCCCGCCAGTAAAGTAAACTTTTTCATCATCATTCCTTAtt                                                                                                   >  1:996055/1‑151 (MQ=255)                                                      cattttttGCTATTTCGTTGGCTTCTGCGAAAGTAATATGGGTTTCTGCCGGAAACCCTTTTTAAATAGTGTAGTCTTGCGCAAAGGTCTTT‑GGTCAAAAAACAAACGGACCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                                  <  1:89340/148‑1 (MQ=255)                                                      cAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                                  >  1:1009338/1‑151 (MQ=255)                                                        gTTTTTGCCATTTCGTTGGCTTCTGCGTAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCGGCGCCCAGGTAATT‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAtt                                                                                                   <  1:254552/148‑1 (MQ=255)                                                         tttttttctttttGTTGTTTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCTTTTTAAATATTGTAGCCCTGCCCCCAGTTATTT‑GGTGCAAAAACAAAAAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCa                                                                                                <  1:797014/141‑1 (MQ=255)                                                         tttttGCCTTTTCGTTGGTTTCTGCGAAATAAATATGGGTTTCTGACGCCAACCCTTTTTAAATTTGGTAGCCCTGCCCCCAGGTCATT‑GGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                                  <  2:317268/148‑1 (MQ=255)                                                         tttttCCCATTTCGTTGTTTTCTGCGCAGGAAATAGAGGTTTCTGTCGGAAACCCTTTTTAAATATTGTAGCCCTACGCAGAGGTCATG‑AGTGAAAAAAAAACCAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTc                                                                                                 <  1:1155898/149‑1 (MQ=255)                                                          ttttGCCTTTTCGTTGGCTTCTGCGAAATAAATATAGTTTTCTGCCGGCAAACCTTTTTTAATTTTGTAGCCCTGCGCCCAGGTCATT‑GGTGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAt                                                                                               <  2:483408/150‑1 (MQ=255)                                                               ccttttCGTTGGCTTCTGCGAAATAAATATAGGTTTCTGACGGAAACCCTTTTTTAATATTGTAGTCCTGCGCCAAGTTCTTT‑GGTGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtttt                                                                                            <  1:188743/145‑1 (MQ=255)                                                               ccATTTCGTTGGCTTCTGCGCAAGTAATATGGGTTTCTGCCGGCAAACCGTTTTTAATATTGTAGTCCTGCCCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAc                                                                                          <  2:991176/150‑1 (MQ=255)                                                                 aTTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAccc                                                                                        >  2:21443/1‑150 (MQ=255)                                                                       ttGGTTTCTGGGAAAGAAATATAGTTTTCTGACGGCAACCCTTTTTTAATATTGTGGTCCGGCGCCAATTTCTTG‑GGTGAAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAg                                                                                      <  1:991815/146‑1 (MQ=255)                                                                       ttGGTTTCTGCGAAAGTAATATAGGTTTCTGACGGCAAACCTTTTTTAATATTGTAGCCCTGCGCCCAGGTCATT‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                                  <  2:167947/150‑1 (MQ=255)                                                                       ttGGCTTCTGCTCAAAAAATATAGTTTTCTGACGGCAATCCTTTTTAAATATTGTCGTCCTGCGCCCAGTTTTTT‑GGTGAAAAAAAAAACTGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                                   <  1:447686/149‑1 (MQ=255)                                                                         ggtttCTGCGCAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTGGCCCTGCGCCCAGGTCATT‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                                <  2:555265/147‑1 (MQ=255)                                                                            ttCTGCGCAAGTAATATGGTTTTCTGACGGAAAACCTTTTTTAATTTTGTAGTCCTGCGCCCAGTTCATT‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacc                                                                             <  2:338814/150‑1 (MQ=255)                                                                            ttCTGCGAAAGTAATATAGGTTTCTGGCGGCAAACCTTTTTTAATATTGTAGTCCTGCGCCCAGGTCTTT‑GGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGca                                                                               <  2:1215736/148‑1 (MQ=255)                                                                               tGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCAccc                                                                         >  2:947248/1‑151 (MQ=255)                                                                               tGCGAAAATAATATATTTGTCCGCCGCCAACCCTTTTTAATTGTTGTATCCGTCCGCCCGGTTAATT‑GGTGCAAAAAAATCCAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                                   <  1:886368/141‑1 (MQ=255)                                                                                 cgcAAGTAATATAGTTTTCTGACGGCAACCCTTTTTTAATATTGTAGTCCTGCGCCCAGTTCTTT‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                        <  2:925011/150‑1 (MQ=255)                                                                                  gcAATTAATATGGTTTTCGGAGGGCAACCCTTTTTTAATATTTTAGTCCTCCCCCCAGTTATTG‑GGTGCGAAAAAAACCAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                        <  2:1097246/149‑1 (MQ=255)                                                                                    aaGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTAcc                                                                    >  1:245845/1‑151 (MQ=255)                                                                                    aaGTAATATAGGTTTCTGACGGAAACCCGTTTTAAATATTGTAGCCCTGCCCCCAGGTCTTG‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                        <  2:494955/147‑1 (MQ=255)                                                                                          tataGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAAt                                                              >  2:970219/1‑151 (MQ=255)                                                                                           ataGGTTTCTGAGGGCAACCCTTTTTAAATTTTGAACTCCTCCG‑CCAGGTCCTTGGGGGAGAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATc                                                             <  2:643291/151‑1 (MQ=255)                                                                                            taGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATc                                                             >  1:649707/1‑150 (MQ=255)                                                                                                tttCTGACGGCAACCCTTTTTAAATTTTGTAGCCCTGCCCCCAGGTCTTT‑GGTGCAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCa                                                            <  2:1157556/147‑1 (MQ=255)                                                                                                     gACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCtt                                                   >  2:896217/1‑151 (MQ=255)                                                                                                       cGGCAACCTTTTTTTAATTTTGTAGTCCCGCCCCCAGTTCTTG‑GGTGCAAAAACAAACGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCAcc                                                     <  2:1238775/147‑1 (MQ=255)                                                                                                        ggCAACCCTTTTTTAATATTGTAGTCCTGCCCCCAGGTCATT‑GGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCt                                                    <  2:291688/147‑1 (MQ=255)                                                                                                        ggCAACCCTTTTTAAATATTGTAGCCCTGCCCCCGGGTATTG‑GGTGCAAAAAAAAACGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCt                                                    <  2:757997/147‑1 (MQ=255)                                                                                                           aaccctttttaAATTTTGTAGTCCTGCCCCCAGTTAATG‑GGTGCAAAAACAAACGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAAt                                               <  2:194206/143‑1 (MQ=255)                                                                                                                gTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                        >  1:597530/1‑151 (MQ=255)                                                                                                                gTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                        >  2:666270/1‑151 (MQ=255)                                                                                                                   tttAATATTGTAGTCCTGCGCCAAGGTATTT‑GGTGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTc                                      <  2:435617/150‑1 (MQ=255)                                                                                                                      aaTATGGTATTCTTGCCCCAAGGTAATG‑GGGGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAgg                                  <  2:1163030/151‑1 (MQ=255)                                                                                                                         atTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGcc                                >  1:3977/1‑150 (MQ=255)                                                                                                                         atTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGcc                                >  1:1016020/1‑150 (MQ=255)                                                                                                                             tAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGc                           >  1:128656/1‑151 (MQ=255)                                                                                                                                 ccggcgcCAGGGTCTTT‑GGTGCAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCAtt                             <  2:466132/142‑1 (MQ=255)                                                                                                                                     cgcCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTc                      >  2:253430/1‑148 (MQ=255)                                                                                                                                      gcCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa                   >  1:654857/1‑150 (MQ=255)                                                                                                                                       ccaagtttaTT‑GGTGAAAAAAAAAACGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGaa                  <  1:779075/142‑1 (MQ=255)                                                                                                                                        ccAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACg                >  2:442056/1‑151 (MQ=255)                                                                                                                                         cAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGc               >  1:965155/1‑151 (MQ=255)                                                                                                                                              cATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTg          >  1:1036153/1‑151 (MQ=255)                                                                                                                                                 t‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAc        >  1:695818/1‑150 (MQ=255)                                                                                                                                                     tGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGc     >  1:383187/1‑150 (MQ=255)                                                                                                                                                        gAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCAcc  >  1:317547/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                      GGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222446‑1222745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑