Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,282	0	T	G	43.1%	117.0 / 12.0	73	V526G (GTT→GGT)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (12/29);  new base G (31/0);  total (44/29)
Fisher's exact test for biased strand distribution p-value = 8.49e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCC  >  NC_000913/354132‑354428                                                                                                                                                        |                                                                                                                                                    ccTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑ggtggt                                                                                                                                                     <  1:125281/151‑1 (MQ=255)          ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTgggggg                                                                                                                                              >  1:979911/1‑147 (MQ=255)            ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAg                                                                                                                                          <  2:274933/151‑1 (MQ=255)             gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGa                                                                                                                                         <  1:68255/151‑1 (MQ=255)                   gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTggggggggggggagaag                                                                                                                                       >  1:122393/1‑138 (MQ=255)                     gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATg                                                                                                                                       >  1:530919/1‑145 (MQ=255)                       cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                <  1:815344/150‑1 (MQ=255)                          tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGGAAGATGCGCTGGAAggg                                                                                                                            >  2:440617/1‑151 (MQ=255)                          tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAAGGGCTGGAAggg                                                                                                                            >  1:337501/1‑151 (MQ=255)                           gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc                                                                                                                           <  1:703539/151‑1 (MQ=255)                             cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                            >  1:971436/1‑148 (MQ=255)                             cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                            >  2:14593/1‑148 (MQ=255)                              ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAgg                                                                                                                        >  2:1160089/1‑151 (MQ=255)                               gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGCAgg                                                                                                                        >  1:620316/1‑150 (MQ=255)                               gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTTGGGGGGAAGATGCGCTGGAAGGGCAgg                                                                                                                        >  1:743423/1‑150 (MQ=255)                                   tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAgggggc‑g                                                                                                                  >  2:353443/1‑151 (MQ=255)                                    cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑gg                                                                                                                 <  1:148410/151‑1 (MQ=255)                                    cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑g                                                                                                                  <  1:951906/150‑1 (MQ=255)                                     aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggt                                                                                                                <  2:496036/151‑1 (MQ=255)                                        ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggtgg                                                                                                              <  1:1025070/150‑1 (MQ=255)                                        ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGGTGGAAGGGGAggtggg‑gggggg                                                                                                             >  1:297642/1‑150 (MQ=255)                                         tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggtggc                                                                                                             <  2:190469/150‑1 (MQ=255)                                          aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGgtggc‑ggtggcg                                                                                                            <  2:1225463/150‑1 (MQ=255)                                            tctcGGGCGCACTGACGATGTAATTAGCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGtt                                                                                                          <  1:426098/150‑1 (MQ=255)                                                 ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGCTGGGGGGGAAGATGCGCCGCAAGGGCAGGGGGC‑GGGGGGGTTTTTCa                                                                                                     >  2:206256/1‑150 (MQ=255)                                                 ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGTGGC‑GGTGGCGTTTGTCa                                                                                                     >  2:1096337/1‑150 (MQ=255)                                                  gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCAtt                                                                                                   <  2:1078791/151‑1 (MQ=255)                                                   cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGGGCTGAAAGGGCAGGGGGG‑GGGGGGGTTTGTCAtt                                                                                                   >  2:1161512/1‑150 (MQ=255)                                                   cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGAAAGGGGCGGGGGC‑GGTGGCGGTTGTCCtt                                                                                                   >  2:184202/1‑150 (MQ=255)                                                      aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGa                                                                                               <  1:372107/151‑1 (MQ=255)                                                        tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGGGGTGGAAGGGGAGGGGGG‑GGGGGGGGTTggtatttccga                                                                                              >  1:1038140/1‑140 (MQ=255)                                                        tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGAAAAGGCAGGGGGC‑GGGGGGGGTTGTCATTCCgga                                                                                              >  2:648335/1‑148 (MQ=255)                                                           cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGG‑GGGGGCGGTTTTCATTCCGaaa                                                                                             >  2:435952/1‑148 (MQ=255)                                                              tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGGAAAGGCAGGGGGC‑GGGGGGGGTTGTCATTTCgga                                                                                              >  1:428577/1‑142 (MQ=255)                                                               gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                       <  1:1202573/150‑1 (MQ=255)                                                               gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                       <  2:663585/150‑1 (MQ=255)                                                                tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGAAAGATGCGCTGGAAGGGGGGGTGGC‑GGGGGGGGTTGTTATTCCGCAAGAGAGCg                                                                                      >  1:790702/1‑150 (MQ=255)                                                                 aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                    <  2:223711/151‑1 (MQ=255)                                                                  aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGCAAAGGCAGGTGGG‑GGGGGCGGTTGTCGTTCCGCAagggag                                                                                        >  2:802310/1‑146 (MQ=255)                                                                    tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGGGGTTGTCATTCCGAAAGAGAGCGACAg                                                                                  >  2:1121112/1‑150 (MQ=255)                                                                       cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTTCGAAAAAGAGGGACAGTCTg                                                                              >  2:811175/1‑151 (MQ=255)                                                                       cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAAGCGCTGGAAGGGGAGGGGGG‑GGGGGGGTTTTTCATTCCGAAAGAGAGCg                                                                                      >  1:178273/1‑143 (MQ=255)                                                                        gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                             <  1:1127395/151‑1 (MQ=255)                                                                        gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTTCGGAAGAGAGGGGCAGTCTgg                                                                             >  1:454193/1‑151 (MQ=255)                                                                         ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGGGGGGGC‑GGGGGGGTTTGTTATTTCGAAAAAGAGAGACAGTCTgg                                                                             >  1:827877/1‑150 (MQ=255)                                                                          tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                            <  1:779632/150‑1 (MQ=255)                                                                             cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGC‑GGTGGCGTTTGTCATTTCGAAAGAGAGGGGCAGTCTGGAAg                                                                          >  2:216702/1‑149 (MQ=255)                                                                               ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                       <  2:991484/150‑1 (MQ=255)                                                                               ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGGAAGATGCGCTGAAAGGGCAGGTGGG‑GGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                             >  2:607792/1‑144 (MQ=255)                                                                               ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGGGCCGAAAAGGCAGGGGGCGGGGGGGGTTGGTCTTTCCGAAA‑AGAGGGAAAGGCTggga                                                                           >  1:804127/1‑144 (MQ=255)                                                                                  aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCCGGAAGGGGCGGGGGGG‑GGGGGGGTTTTTCTTTCCGAAA‑AGAGGGGCAGGCTGGAAGATCg                                                                      >  1:517717/1‑148 (MQ=255)                                                                                      gCTGGGGACGCGCGAGATTGACGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGGTGGGGGGGAAGATGCGGTGAAAGGGCAGGGGGC‑GGGGGGGCTTGTAATTCCGCAAGAGGGCGGCAGTCTGGAAGATCGTgtgg                                                                 >  1:1152485/1‑146 (MQ=255)                                                                                      gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                               <  1:181114/151‑1 (MQ=255)                                                                                      gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                               <  1:434953/151‑1 (MQ=255)                                                                                         ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGgcg                                                            >  2:803336/1‑151 (MQ=255)                                                                                              cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                       <  1:191603/151‑1 (MQ=255)                                                                                                   gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                  <  1:440616/151‑1 (MQ=255)                                                                                                   gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGG‑GGGGGGGGTTTTCATTCCGAAAGAGAGCGACAGTCTGGGAGATTGTGATTTGGGGCCCTCGc                                                     >  2:329852/1‑148 (MQ=255)                                                                                                   gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGTTTGGGGGTAAGATGCGCCGAAAGGGCAGGGGGC‑GGTCGCGTTTATCATTACGAAATAAAACGACAGTCTTGAAGATCGTGATGGGGCGCCCTCg                                                      >  1:1005657/1‑147 (MQ=255)                                                                                                     gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                <  2:1197754/151‑1 (MQ=255)                                                                                                     gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAAATGCGCTGGAAGGGCAGGGGGG‑GGGGGGGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGGAGATCGTGATTTGGGGCCCCCGc                                                     >  1:190153/1‑146 (MQ=255)                                                                                                      aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                               <  1:94961/151‑1 (MQ=255)                                                                                                           agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCCTTCCGGAAGAGAGCGGCAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAgggg                                          >  1:1099849/1‑149 (MQ=255)                                                                                                                  aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                   >  2:1124078/1‑151 (MQ=255)                                                                                                                   tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGGAAAATGCGCTGAAAGGGCCGGTGGG‑GGGGGCGTTTGTCGTTTCGGAAGGGAGCGGCAGGCTGGGAGATTGGGGTGTGGGGCACCCGCCAGAGaaaggggttttgg                                   >  2:155887/1‑139 (MQ=255)                                                                                                                     tcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGCGGTTGTCATTTCGCAAGAGAGCGACAGGCTGGGAGATCGTGGTGGGGCGCACTCGCAAGAGAAgggggataatg                                   >  1:1153920/1‑139 (MQ=255)                                                                                                                      ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                >  2:556473/1‑150 (MQ=255)                                                                                                                            aTCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGC‑GGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGACTGGAAGAACGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                          >  2:839359/1‑150 (MQ=255)                                                                                                                              ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                       <  2:1079160/151‑1 (MQ=255)                                                                                                                              ccGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCCGAAAGGGCGGGGGGC‑GGGGGCGGTTGTCATTCCCAAAGAGAGCGGCAGTCTGGGAAAACGTGATGGGGCGCACTCGCGAGAGAAGGCGGTTTTTGCGCtgggggg                         >  1:1070104/1‑148 (MQ=255)                                                                                                                                    gTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAcagacaga                 <  1:92207/151‑1 (MQ=255)                                                                                                                                      tGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGCAAGGGCAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGGGAGTATGGCGCTGGTGGGCAGCCAGCtt               >  1:229267/1‑151 (MQ=255)                                                                                                                                       gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg              <  2:512106/151‑1 (MQ=255)                                                                                                                                         cGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGG‑GGGGGGGGTTGTTATTCCGAAAGAGAGGGGCAGTCTGGGAGATCGTGGTGGGGGGCACTCGCCAGAGAAGGGGGTTATGGGGGTGGGGGACAGCCAGATTgg             >  1:24531/1‑150 (MQ=255)                                                                                                                                            aGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc         <  2:735858/151‑1 (MQ=255)                                                                                                                                                    gtggtTGGGGTGAAAGATGCGCTGAAAGGGCATGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc  <  1:1034366/151‑1 (MQ=255)                                                                                                                                                     tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc  >  1:1056213/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                    CCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCC  >  NC_000913/354132‑354428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑