Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	51.3%	93.0 / 130.1	76	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (6/31);  new base G (39/0);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 1.21e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-12

GGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354143‑354435                                                                                                                                                   |                                                                                                                                                     ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAg                                                                                                                                                 <  2:274933/151‑1 (MQ=255)  gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGa                                                                                                                                                <  1:68255/151‑1 (MQ=255)          gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATg                                                                                                                                              >  1:530919/1‑145 (MQ=255)            cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                       <  1:815344/150‑1 (MQ=255)               tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGGAAGATGCGCTGGAAggg                                                                                                                                   >  2:440617/1‑151 (MQ=255)               tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAAGGGCTGGAAggg                                                                                                                                   >  1:337501/1‑151 (MQ=255)                gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc                                                                                                                                  <  1:703539/151‑1 (MQ=255)                  cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                                   >  1:971436/1‑148 (MQ=255)                  cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                                   >  2:14593/1‑148 (MQ=255)                   ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAgg                                                                                                                               >  2:1160089/1‑151 (MQ=255)                    gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGCAgg                                                                                                                               >  1:620316/1‑150 (MQ=255)                    gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTTGGGGGGAAGATGCGCTGGAAGGGCAgg                                                                                                                               >  1:743423/1‑150 (MQ=255)                        tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAgggggc‑g                                                                                                                         >  2:353443/1‑151 (MQ=255)                         cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑gg                                                                                                                        <  1:148410/151‑1 (MQ=255)                         cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑g                                                                                                                         <  1:951906/150‑1 (MQ=255)                          aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggt                                                                                                                       <  2:496036/151‑1 (MQ=255)                             ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggtgg                                                                                                                     <  1:1025070/150‑1 (MQ=255)                             ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGGTGGAAGGGGAggtggg‑gggggg                                                                                                                    >  1:297642/1‑150 (MQ=255)                              tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggtggc                                                                                                                    <  2:190469/150‑1 (MQ=255)                               aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGgtggc‑ggtggcg                                                                                                                   <  2:1225463/150‑1 (MQ=255)                                 tctcGGGCGCACTGACGATGTAATTAGCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGtt                                                                                                                 <  1:426098/150‑1 (MQ=255)                                      ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGCTGGGGGGGAAGATGCGCCGCAAGGGCAGGGGGC‑GGGGGGGTTTTTCa                                                                                                            >  2:206256/1‑150 (MQ=255)                                      ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGTGGC‑GGTGGCGTTTGTCa                                                                                                            >  2:1096337/1‑150 (MQ=255)                                       gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCAtt                                                                                                          <  2:1078791/151‑1 (MQ=255)                                        cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGGGCTGAAAGGGCAGGGGGG‑GGGGGGGTTTGTCAtt                                                                                                          >  2:1161512/1‑150 (MQ=255)                                        cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGAAAGGGGCGGGGGC‑GGTGGCGGTTGTCCtt                                                                                                          >  2:184202/1‑150 (MQ=255)                                           aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGa                                                                                                      <  1:372107/151‑1 (MQ=255)                                             tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGGGGTGGAAGGGGAGGGGGG‑GGGGGGGGTTggtatttccga                                                                                                     >  1:1038140/1‑140 (MQ=255)                                             tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGAAAAGGCAGGGGGC‑GGGGGGGGTTGTCATTCCgga                                                                                                     >  2:648335/1‑148 (MQ=255)                                                cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGG‑GGGGGCGGTTTTCATTCCGaaa                                                                                                    >  2:435952/1‑148 (MQ=255)                                                   tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGGAAAGGCAGGGGGC‑GGGGGGGGTTGTCATTTCgga                                                                                                     >  1:428577/1‑142 (MQ=255)                                                    gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                              <  1:1202573/150‑1 (MQ=255)                                                    gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                              <  2:663585/150‑1 (MQ=255)                                                     tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGAAAGATGCGCTGGAAGGGGGGGTGGC‑GGGGGGGGTTGTTATTCCGCAAGAGAGCg                                                                                             >  1:790702/1‑150 (MQ=255)                                                      aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                           <  2:223711/151‑1 (MQ=255)                                                       aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGCAAAGGCAGGTGGG‑GGGGGCGGTTGTCGTTCCGCAagggag                                                                                               >  2:802310/1‑146 (MQ=255)                                                         tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGGGGTTGTCATTCCGAAAGAGAGCGACAg                                                                                         >  2:1121112/1‑150 (MQ=255)                                                            cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTTCGAAAAAGAGGGACAGTCTg                                                                                     >  2:811175/1‑151 (MQ=255)                                                            cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAAGCGCTGGAAGGGGAGGGGGG‑GGGGGGGTTTTTCATTCCGAAAGAGAGCg                                                                                             >  1:178273/1‑143 (MQ=255)                                                             gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                    <  1:1127395/151‑1 (MQ=255)                                                             gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTTCGGAAGAGAGGGGCAGTCTgg                                                                                    >  1:454193/1‑151 (MQ=255)                                                              ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGGGGGGGC‑GGGGGGGTTTGTTATTTCGAAAAAGAGAGACAGTCTgg                                                                                    >  1:827877/1‑150 (MQ=255)                                                               tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                   <  1:779632/150‑1 (MQ=255)                                                                  cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGC‑GGTGGCGTTTGTCATTTCGAAAGAGAGGGGCAGTCTGGAAg                                                                                 >  2:216702/1‑149 (MQ=255)                                                                    ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                              <  2:991484/150‑1 (MQ=255)                                                                    ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGGAAGATGCGCTGAAAGGGCAGGTGGG‑GGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                    >  2:607792/1‑144 (MQ=255)                                                                    ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGGGCCGAAAAGGCAGGGGGCGGGGGGGGTTGGTCTTTCCGAAA‑AGAGGGAAAGGCTggga                                                                                  >  1:804127/1‑144 (MQ=255)                                                                       aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCCGGAAGGGGCGGGGGGG‑GGGGGGGTTTTTCTTTCCGAAA‑AGAGGGGCAGGCTGGAAGATCg                                                                             >  1:517717/1‑148 (MQ=255)                                                                           gCTGGGGACGCGCGAGATTGACGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGGTGGGGGGGAAGATGCGGTGAAAGGGCAGGGGGC‑GGGGGGGCTTGTAATTCCGCAAGAGGGCGGCAGTCTGGAAGATCGTgtgg                                                                        >  1:1152485/1‑146 (MQ=255)                                                                           gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                      <  1:181114/151‑1 (MQ=255)                                                                           gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                      <  1:434953/151‑1 (MQ=255)                                                                              ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGgcg                                                                   >  2:803336/1‑151 (MQ=255)                                                                                   cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                              <  1:191603/151‑1 (MQ=255)                                                                                        gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                         <  1:440616/151‑1 (MQ=255)                                                                                        gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGG‑GGGGGGGGTTTTCATTCCGAAAGAGAGCGACAGTCTGGGAGATTGTGATTTGGGGCCCTCGc                                                            >  2:329852/1‑148 (MQ=255)                                                                                        gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGTTTGGGGGTAAGATGCGCCGAAAGGGCAGGGGGC‑GGTCGCGTTTATCATTACGAAATAAAACGACAGTCTTGAAGATCGTGATGGGGCGCCCTCg                                                             >  1:1005657/1‑147 (MQ=255)                                                                                          gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                       <  2:1197754/151‑1 (MQ=255)                                                                                          gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAAATGCGCTGGAAGGGCAGGGGGG‑GGGGGGGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGGAGATCGTGATTTGGGGCCCCCGc                                                            >  1:190153/1‑146 (MQ=255)                                                                                           aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                      <  1:94961/151‑1 (MQ=255)                                                                                                agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCCTTCCGGAAGAGAGCGGCAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAgggg                                                 >  1:1099849/1‑149 (MQ=255)                                                                                                       aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                          >  2:1124078/1‑151 (MQ=255)                                                                                                        tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGGAAAATGCGCTGAAAGGGCCGGTGGG‑GGGGGCGTTTGTCGTTTCGGAAGGGAGCGGCAGGCTGGGAGATTGGGGTGTGGGGCACCCGCCAGAGaaaggggttttgg                                          >  2:155887/1‑139 (MQ=255)                                                                                                          tcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGCGGTTGTCATTTCGCAAGAGAGCGACAGGCTGGGAGATCGTGGTGGGGCGCACTCGCAAGAGAAgggggataatg                                          >  1:1153920/1‑139 (MQ=255)                                                                                                           ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                       >  2:556473/1‑150 (MQ=255)                                                                                                                 aTCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGC‑GGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGACTGGAAGAACGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                                 >  2:839359/1‑150 (MQ=255)                                                                                                                   ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                              <  2:1079160/151‑1 (MQ=255)                                                                                                                   ccGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCCGAAAGGGCGGGGGGC‑GGGGGCGGTTGTCATTCCCAAAGAGAGCGGCAGTCTGGGAAAACGTGATGGGGCGCACTCGCGAGAGAAGGCGGTTTTTGCGCtgggggg                                >  1:1070104/1‑148 (MQ=255)                                                                                                                         gTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAcagacaga                        <  1:92207/151‑1 (MQ=255)                                                                                                                           tGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGCAAGGGCAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGGGAGTATGGCGCTGGTGGGCAGCCAGCtt                      >  1:229267/1‑151 (MQ=255)                                                                                                                            gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                     <  2:512106/151‑1 (MQ=255)                                                                                                                              cGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGG‑GGGGGGGGTTGTTATTCCGAAAGAGAGGGGCAGTCTGGGAGATCGTGGTGGGGGGCACTCGCCAGAGAAGGGGGTTATGGGGGTGGGGGACAGCCAGATTgg                    >  1:24531/1‑150 (MQ=255)                                                                                                                                 aGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc                <  2:735858/151‑1 (MQ=255)                                                                                                                                         gtggtTGGGGTGAAAGATGCGCTGAAAGGGCATGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc         <  1:1034366/151‑1 (MQ=255)                                                                                                                                          tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc         >  1:1056213/1‑150 (MQ=255)                                                                                                                                              tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg    >  1:69190/1‑151 (MQ=255)                                                                                                                                                gggTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc  <  1:480630/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354143‑354435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑