Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,318	0	T	G	40.6%	133.5 / 11.5	69	V538G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (17/24);  new base G (28/0);  total (45/24)
Fisher's exact test for biased strand distribution p-value = 7.14e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCA  >  NC_000913/354168‑354463                                                                                                                                                         |                                                                                                                                                  aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggt                                                                                                                                                   <  2:496036/151‑1 (MQ=255)    ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggtgg                                                                                                                                                 <  1:1025070/150‑1 (MQ=255)    ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGGTGGAAGGGGAggtggg‑gggggg                                                                                                                                                >  1:297642/1‑150 (MQ=255)     tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc‑ggtggc                                                                                                                                                <  2:190469/150‑1 (MQ=255)      aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGgtggc‑ggtggcg                                                                                                                                               <  2:1225463/150‑1 (MQ=255)        tctcGGGCGCACTGACGATGTAATTAGCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGtt                                                                                                                                             <  1:426098/150‑1 (MQ=255)             ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGCTGGGGGGGAAGATGCGCCGCAAGGGCAGGGGGC‑GGGGGGGTTTTTCa                                                                                                                                        >  2:206256/1‑150 (MQ=255)             ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGTGGC‑GGTGGCGTTTGTCa                                                                                                                                        >  2:1096337/1‑150 (MQ=255)              gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCAtt                                                                                                                                      <  2:1078791/151‑1 (MQ=255)               cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGGGCTGAAAGGGCAGGGGGG‑GGGGGGGTTTGTCAtt                                                                                                                                      >  2:1161512/1‑150 (MQ=255)               cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGAAAGGGGCGGGGGC‑GGTGGCGGTTGTCCtt                                                                                                                                      >  2:184202/1‑150 (MQ=255)                  aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGa                                                                                                                                  <  1:372107/151‑1 (MQ=255)                    tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGGGGTGGAAGGGGAGGGGGG‑GGGGGGGGTTggtatttccga                                                                                                                                 >  1:1038140/1‑140 (MQ=255)                    tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGAAAAGGCAGGGGGC‑GGGGGGGGTTGTCATTCCgga                                                                                                                                 >  2:648335/1‑148 (MQ=255)                       cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGG‑GGGGGCGGTTTTCATTCCGaaa                                                                                                                                >  2:435952/1‑148 (MQ=255)                          tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGGAAAGGCAGGGGGC‑GGGGGGGGTTGTCATTTCgga                                                                                                                                 >  1:428577/1‑142 (MQ=255)                           gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                                                          <  2:663585/150‑1 (MQ=255)                           gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                                                          <  1:1202573/150‑1 (MQ=255)                            tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGAAAGATGCGCTGGAAGGGGGGGTGGC‑GGGGGGGGTTGTTATTCCGCAAGAGAGCg                                                                                                                         >  1:790702/1‑150 (MQ=255)                             aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                                                       <  2:223711/151‑1 (MQ=255)                              aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGCAAAGGCAGGTGGG‑GGGGGCGGTTGTCGTTCCGCAagggag                                                                                                                           >  2:802310/1‑146 (MQ=255)                                tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGGGGTTGTCATTCCGAAAGAGAGCGACAg                                                                                                                     >  2:1121112/1‑150 (MQ=255)                                   cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTTCGAAAAAGAGGGACAGTCTg                                                                                                                 >  2:811175/1‑151 (MQ=255)                                   cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAAGCGCTGGAAGGGGAGGGGGG‑GGGGGGGTTTTTCATTCCGAAAGAGAGCg                                                                                                                         >  1:178273/1‑143 (MQ=255)                                    gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                                                <  1:1127395/151‑1 (MQ=255)                                    gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCATTTCGGAAGAGAGGGGCAGTCTgg                                                                                                                >  1:454193/1‑151 (MQ=255)                                     ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGGGGGGGC‑GGGGGGGTTTGTTATTTCGAAAAAGAGAGACAGTCTgg                                                                                                                >  1:827877/1‑150 (MQ=255)                                      tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                                               <  1:779632/150‑1 (MQ=255)                                         cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGC‑GGTGGCGTTTGTCATTTCGAAAGAGAGGGGCAGTCTGGAAg                                                                                                             >  2:216702/1‑149 (MQ=255)                                           ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                                                          <  2:991484/150‑1 (MQ=255)                                           ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGGAAGATGCGCTGAAAGGGCAGGTGGG‑GGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                                                >  2:607792/1‑144 (MQ=255)                                           ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGGGCCGAAAAGGCAGGGGGCGGGGGGGGTTGGTCTTTCCGAAA‑AGAGGGAAAGGCTggga                                                                                                              >  1:804127/1‑144 (MQ=255)                                              aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCCGGAAGGGGCGGGGGGG‑GGGGGGGTTTTTCTTTCCGAAA‑AGAGGGGCAGGCTGGAAGATCg                                                                                                         >  1:517717/1‑148 (MQ=255)                                                  gCTGGGGACGCGCGAGATTGACGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGGTGGGGGGGAAGATGCGGTGAAAGGGCAGGGGGC‑GGGGGGGCTTGTAATTCCGCAAGAGGGCGGCAGTCTGGAAGATCGTgtgg                                                                                                    >  1:1152485/1‑146 (MQ=255)                                                  gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                                                  <  1:181114/151‑1 (MQ=255)                                                  gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                                                  <  1:434953/151‑1 (MQ=255)                                                     ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGgcg                                                                                               >  2:803336/1‑151 (MQ=255)                                                          cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                                                          <  1:191603/151‑1 (MQ=255)                                                               gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                                                     <  1:440616/151‑1 (MQ=255)                                                               gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGG‑GGGGGGGGTTTTCATTCCGAAAGAGAGCGACAGTCTGGGAGATTGTGATTTGGGGCCCTCGc                                                                                        >  2:329852/1‑148 (MQ=255)                                                               gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGAGGTTTGGGGGTAAGATGCGCCGAAAGGGCAGGGGGC‑GGTCGCGTTTATCATTACGAAATAAAACGACAGTCTTGAAGATCGTGATGGGGCGCCCTCg                                                                                         >  1:1005657/1‑147 (MQ=255)                                                                 gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                                                   <  2:1197754/151‑1 (MQ=255)                                                                 gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAAATGCGCTGGAAGGGCAGGGGGG‑GGGGGGGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGGAGATCGTGATTTGGGGCCCCCGc                                                                                        >  1:190153/1‑146 (MQ=255)                                                                  aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                                                  <  1:94961/151‑1 (MQ=255)                                                                       agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGG‑GGGGGGGGTTGTCCTTCCGGAAGAGAGCGGCAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAgggg                                                                             >  1:1099849/1‑149 (MQ=255)                                                                              aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                                                      >  2:1124078/1‑151 (MQ=255)                                                                               tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGGAAAATGCGCTGAAAGGGCCGGTGGG‑GGGGGCGTTTGTCGTTTCGGAAGGGAGCGGCAGGCTGGGAGATTGGGGTGTGGGGCACCCGCCAGAGaaaggggttttgg                                                                      >  2:155887/1‑139 (MQ=255)                                                                                 tcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGC‑GGGGGCGGTTGTCATTTCGCAAGAGAGCGACAGGCTGGGAGATCGTGGTGGGGCGCACTCGCAAGAGAAgggggataatg                                                                      >  1:1153920/1‑139 (MQ=255)                                                                                  ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                                                   >  2:556473/1‑150 (MQ=255)                                                                                        aTCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGC‑GGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGACTGGAAGAACGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                                                             >  2:839359/1‑150 (MQ=255)                                                                                          ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                                                          <  2:1079160/151‑1 (MQ=255)                                                                                          ccGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCCGAAAGGGCGGGGGGC‑GGGGGCGGTTGTCATTCCCAAAGAGAGCGGCAGTCTGGGAAAACGTGATGGGGCGCACTCGCGAGAGAAGGCGGTTTTTGCGCtgggggg                                                            >  1:1070104/1‑148 (MQ=255)                                                                                                gTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAcagacaga                                                    <  1:92207/151‑1 (MQ=255)                                                                                                  tGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGCAAGGGCAGGGGGC‑GGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGGGAGTATGGCGCTGGTGGGCAGCCAGCtt                                                  >  1:229267/1‑151 (MQ=255)                                                                                                   gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                                                 <  2:512106/151‑1 (MQ=255)                                                                                                     cGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGG‑GGGGGGGGTTGTTATTCCGAAAGAGAGGGGCAGTCTGGGAGATCGTGGTGGGGGGCACTCGCCAGAGAAGGGGGTTATGGGGGTGGGGGACAGCCAGATTgg                                                >  1:24531/1‑150 (MQ=255)                                                                                                        aGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc                                            <  2:735858/151‑1 (MQ=255)                                                                                                                gtggtTGGGGTGAAAGATGCGCTGAAAGGGCATGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc                                     <  1:1034366/151‑1 (MQ=255)                                                                                                                 tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc                                     >  1:1056213/1‑150 (MQ=255)                                                                                                                     tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg                                >  1:69190/1‑151 (MQ=255)                                                                                                                       gggTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc                              <  1:480630/151‑1 (MQ=255)                                                                                                                            aaaGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACg                         >  2:1111789/1‑151 (MQ=255)                                                                                                                               gATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCt                      >  2:248451/1‑151 (MQ=255)                                                                                                                                  gcgcTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGCCGCCCCGGCGAACGCCGGGt                   >  1:984979/1‑151 (MQ=255)                                                                                                                                   cgcTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGtt                  >  2:649781/1‑151 (MQ=255)                                                                                                                                   cgcTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGtt                  <  2:743425/151‑1 (MQ=255)                                                                                                                                    gcTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAAAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGttt                 >  2:417708/1‑151 (MQ=255)                                                                                                                                     cTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTg                >  1:42283/1‑151 (MQ=255)                                                                                                                                     cTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTg                >  1:852920/1‑151 (MQ=255)                                                                                                                                      tGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGttt                 >  2:84092/1‑149 (MQ=255)                                                                                                                                       gAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGtc              <  2:1244446/151‑1 (MQ=255)                                                                                                                                         aaGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGtctc            >  1:158190/1‑151 (MQ=255)                                                                                                                                               aGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAAtt      <  2:337501/151‑1 (MQ=255)                                                                                                                                               aGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAAtt      >  1:510632/1‑151 (MQ=255)                                                                                                                                                ggtggc‑ggtggcGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTg     <  1:1096329/151‑1 (MQ=255)                                                                                                                                                   ggc‑ggtggcgTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCa  <  1:1199889/151‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                  ACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGC‑GGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCA  >  NC_000913/354168‑354463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑