Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,378	G→T	R218S (CGT→AGT)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,378	0	G	T	100.0%	251.5 / NA	62	R218S (CGT→AGT)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/28);  total (34/28)

CAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCC  >  NC_000913/3440231‑3440525                                                                                                                                                    |                                                                                                                                                    cAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAAt                                                                                                                                                  >  2:643253/1‑151 (MQ=255)  agagCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATc                                                                                                                                                 <  2:567057/151‑1 (MQ=255)        gACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCctct                                                                                                                                           <  1:1264282/151‑1 (MQ=255)           aGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTc                                                                                                                                         >  1:260808/1‑150 (MQ=255)            gTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAg                                                                                                                                       <  1:910438/151‑1 (MQ=255)            gTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAg                                                                                                                                       <  1:878529/151‑1 (MQ=255)              cAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGa                                                                                                                                     <  1:868063/151‑1 (MQ=255)               aaTTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGAt                                                                                                                                    <  2:800855/151‑1 (MQ=255)                aTTCCAGATCGTCAACAGGGCGCAGCAGGATCGGAGCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGGCGTACATCACGTAAGACAACGAAAGCTGCCAGTTGTTCAGGCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATc                                                                                                                                   <  1:932734/151‑1 (MQ=255)                    cAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGAtt                                                                                                                               <  1:1116398/151‑1 (MQ=255)                    cAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGAtt                                                                                                                               <  1:8821/151‑1 (MQ=255)                     aGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATtg                                                                                                                              >  1:1063359/1‑151 (MQ=255)                      gATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATtg                                                                                                                              >  2:100230/1‑150 (MQ=255)                       aTCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATtgtg                                                                                                                            >  2:495950/1‑151 (MQ=255)                          gTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCg                                                                                                                         <  1:575352/151‑1 (MQ=255)                              aCAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTgg                                                                                                                     >  2:200126/1‑151 (MQ=255)                              aCAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTgg                                                                                                                     <  1:681578/151‑1 (MQ=255)                               cAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGt                                                                                                                    <  2:368527/151‑1 (MQ=255)                               cAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGt                                                                                                                    >  2:1045727/1‑151 (MQ=255)                                     gcagcaGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCAt                                                                                                              <  2:700791/151‑1 (MQ=255)                                       agcagGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCAttt                                                                                                            <  1:138851/151‑1 (MQ=255)                                        gcagGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTc                                                                                                           >  1:1217199/1‑151 (MQ=255)                                          agGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCg                                                                                                          >  1:451803/1‑150 (MQ=255)                                           ggatcggatcGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGAt                                                                                                        >  2:53433/1‑151 (MQ=255)                                            gatcggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATg                                                                                                       <  2:64101/151‑1 (MQ=255)                                             atcggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGa                                                                                                      <  1:32966/151‑1 (MQ=255)                                               cggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGAcc                                                                                                    >  2:1157927/1‑151 (MQ=255)                                                ggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGAcc                                                                                                    >  2:1256114/1‑150 (MQ=255)                                                  atcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGc                                                                                                 >  1:53890/1‑151 (MQ=255)                                                      aaCTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGt                                                                                             >  2:827651/1‑151 (MQ=255)                                                             gTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTc                                                                                      <  1:522487/151‑1 (MQ=255)                                                               ttCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCgg                                                                                    >  2:445882/1‑151 (MQ=255)                                                                 ctctTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACTACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGt                                                                                   >  2:1133246/1‑150 (MQ=255)                                                                     tcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGc                                                                               >  1:47603/1‑150 (MQ=255)                                                                        ttCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTg                                                                             <  1:323724/149‑1 (MQ=255)                                                                         tCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGtt                                                                           >  1:753380/1‑150 (MQ=255)                                                                         tCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGtt                                                                           <  1:409923/150‑1 (MQ=255)                                                                          cACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCt                                                                         >  2:1087855/1‑151 (MQ=255)                                                                           aCTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTa                                                                        <  2:451960/151‑1 (MQ=255)                                                                              tCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTacac                                                                     >  1:1038991/1‑151 (MQ=255)                                                                               cAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTaca                                                                      <  2:919853/149‑1 (MQ=255)                                                                                     gACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCgct                                                                <  2:1214618/149‑1 (MQ=255)                                                                                           cATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAAc                                                        >  1:870259/1‑151 (MQ=255)                                                                                             tCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACAt                                                      >  1:951118/1‑151 (MQ=255)                                                                                                cGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGt                                                   >  1:474430/1‑151 (MQ=255)                                                                                                  tAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGt                                                   >  1:8382/1‑149 (MQ=255)                                                                                                   aaGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAgg                                                <  2:95185/151‑1 (MQ=255)                                                                                                    aGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGc                                               >  2:1157102/1‑151 (MQ=255)                                                                                                       cAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAAt                                            >  2:100648/1‑151 (MQ=255)                                                                                                            aaaGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGc                                        >  1:35465/1‑150 (MQ=255)                                                                                                            aaaGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCt                                       >  1:650058/1‑151 (MQ=255)                                                                                                                cTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCcac                                   >  2:326376/1‑151 (MQ=255)                                                                                                                 ttCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCcac                                   >  2:1037889/1‑150 (MQ=255)                                                                                                                  tCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCcaca                                  <  2:1191142/150‑1 (MQ=255)                                                                                                                      gttgttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCt                             >  1:324488/1‑151 (MQ=255)                                                                                                                         gttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTa                          <  2:1063367/151‑1 (MQ=255)                                                                                                                           tCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAg                         <  1:990854/150‑1 (MQ=255)                                                                                                                                cAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCg                   >  2:97622/1‑151 (MQ=255)                                                                                                                                  gAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTc                 >  1:174405/1‑151 (MQ=255)                                                                                                                                         tGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcag           <  1:53433/150‑1 (MQ=255)                                                                                                                                             gCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGAc      >  1:804007/1‑151 (MQ=255)                                                                                                                                              cACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACg     <  1:1135929/151‑1 (MQ=255)                                                                                                                                                cgactaATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTTTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGc   <  1:100230/151‑1 (MQ=255)                                                                                                                                                 gactaATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGcc  >  1:820763/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCC  >  NC_000913/3440231‑3440525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑