Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,001,164	0	A	C	46.3%	53.4 / 13.7	41	T274P (ACC→CCC)	xdhA	xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base A (20/2);  new base C (0/19);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 9.40e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGT  >  NC_000913/3001015‑3001312                                                                                                                                                          |                                                                                                                                                     cATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAAc                                                                                                                                                     >  1:581917/1‑151 (MQ=255)  aTGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAAcc                                                                                                                                                    >  2:792631/1‑151 (MQ=255)    gCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCg                                                                                                                                                  <  2:943991/151‑1 (MQ=255)                    aCCATTTGTCGGTGGCGGTTTTGGTAATAAACAAGATGTACTGGAAGAGCCAATGGCGGCATTTCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑CTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCtttt                                                                                                                                  >  2:803399/1‑151 (MQ=255)                                             aataaACAGGATGTACGGGAAGACCAATTGGCGGCATCCCGCCCCAGCAAGTTGGGCGGCCTCCGGGTAAATG‑TTTCCC‑TTACCG‑GGG‑AAGAGTGTTCCCGCCCACCCCTTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGa                                                                                                         <  2:987/151‑1 (MQ=255)                                             aaaaaaCGGAATGTGCGGGAAGAGCAAAGGGCGGCTTTCCTGACCAGCAGGCTGGGCGGCTTTCGGGTAAAAT‑TTCCCC‑TTGGCG‑GTG‑AAGAGTGTTCCCTCGCCCCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGc                                                                                                             <  2:75279/144‑1 (MQ=255)                                             aaaaaaCAGGTTTTAGTGGAAGAGCCATTGGCGGCTTTCCTGACCAACAAGCTGGGCGGCTTCCCGGTAAATT‑TTCCCC‑TTCGCC‑GTG‑AAGAGTTTTTCCTCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGt                                                                                                           <  2:494366/146‑1 (MQ=255)                                                 aggaGGTTGTACGGAAAGAGCCATTGGGGGCATCCTTGACAAGAAAGTTGGCCGGCATCCCGGT‑AAAT‑TTTCCC‑TTTGCCGGGG‑AAAGGTTTTCCCCCGCACCCCTTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcg                                                                                                     <  1:348539/148‑1 (MQ=255)                                                     ggATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGAc                                                                                                 >  2:413836/1‑151 (MQ=255)                                                       aTGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACgg                                                                                               >  2:671700/1‑151 (MQ=255)                                                             tGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACAtt                                                                                         >  2:845888/1‑151 (MQ=255)                                                              ggAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAATG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTg                                                                                        >  2:612804/1‑151 (MQ=255)                                                              ggAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACAtt                                                                                         >  2:677446/1‑150 (MQ=255)                                                               aaaGACCCAATGGCGGCATTCATAACCAGGATGTTTGGCGGC‑GTCCGGAAAAAA‑TTTCCC‑CTTGCCTGTG‑AGGGTTGTTTCCCCCCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACg                                                                                                <  2:927685/141‑1 (MQ=255)                                                                  gagCCAATGGGGGCTTTCCTAACCGGCAGTTTTGGGGGTATCCCGGAAAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGGTTTTTCCCTCAAACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaaa                                                                                     <  1:882046/150‑1 (MQ=255)                                                                     ccAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGtt                                                                                 >  2:78263/1‑151 (MQ=255)                                                                          ggcggcTTCCCTGACCAGAAAGTTGGGCGGCATCCCGGTAAAAT‑TTTCCC‑TGGGCG‑GGG‑AAGAGTTTTCCCTCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGtt                                                                                 <  1:389363/146‑1 (MQ=255)                                                                          ggcggcATCCCTGACCAGAAGGCTGGGCGCCTTTCGGGGAATTT‑TTTCCT‑TTGGCC‑GTA‑AGGTGTTTTCCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGtt                                                                                 <  1:612806/146‑1 (MQ=255)                                                                                 ttCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATg                                                                     >  1:732896/1‑151 (MQ=255)                                                                                      gACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTg                                                                >  2:237038/1‑151 (MQ=255)                                                                                          aGCAAGCTTGGCGGCATTCCGGGGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAAAGTGTTTCCTCTCAACCCGTACCCGCCACGCTTGTACCATTGACGGGCAAATGGGCGTGACCCGCGACGCAACATTGAAAGGTTATAGTCTGGATGTTCTGt                                                               >  2:35307/1‑148 (MQ=255)                                                                                            cAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAac                                                          >  1:711157/1‑151 (MQ=255)                                                                                                   gggggcATGCGGGTAAAAG‑TTCCCC‑TTAGCC‑GTA‑AAGGTTGTTCCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGgcg                                                   <  1:8353/148‑1 (MQ=255)                                                                                                   ggcggcATTCGGGAAAAAT‑TTCCCC‑TTAGCC‑GGA‑AAGGGTTTTCCCTCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGgcg                                                   <  1:370886/151‑1 (MQ=255)                                                                                                   ggcggc‑TTTCGGTTAAAGTTTTCCC‑TTGGCC‑GAG‑AAGAGTTTTCCCTCGCACCCCTTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCg                                                      <  1:4688/148‑1 (MQ=255)                                                                                                    ggcgcttttcGGTGAAAA‑TTTCCC‑TTTACC‑GGGAAAGATTTTTCCCCCGAACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCgg                                                     <  2:166152/140‑1 (MQ=255)                                                                                                       ggcttCCGGGTAAAA‑TTTCCCTTTAGCG‑GGG‑AGGAGTTTTCCCCCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCtt                                                <  1:948944/148‑1 (MQ=255)                                                                                                              ggTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCAtct                                        >  1:599309/1‑151 (MQ=255)                                                                                                                 gAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCAc                                     >  2:334928/1‑151 (MQ=255)                                                                                                                 gAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCAc                                     >  2:666291/1‑151 (MQ=255)                                                                                                                          ccc‑TTAGCG‑GTG‑AAGGTTTTTCCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCAc                               <  1:689879/149‑1 (MQ=255)                                                                                                                          ccc‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTc                             >  2:332517/1‑151 (MQ=255)                                                                                                                            ccctgggcgggg‑AAGATTTTTTCCCCCCCCCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACt                              <  2:433866/139‑1 (MQ=255)                                                                                                                            c‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTcc                            >  2:711129/1‑150 (MQ=255)                                                                                                                                    ccgtgaggtttttttCCTCGCACCCAGTGCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCt                      <  1:303914/139‑1 (MQ=255)                                                                                                                                    ccgggaatggtttttCCGCGCCCCCCGACCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCg                        <  2:104568/137‑1 (MQ=255)                                                                                                                                       g‑AAGAGTGTTTCCTCGCAACACGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTg                  >  2:752517/1‑151 (MQ=255)                                                                                                                                           ggtttttcccTCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTg                  <  2:794589/140‑1 (MQ=255)                                                                                                                                           gggttttcccccGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCATTCCATCGCTTCTGc                 <  1:929152/138‑1 (MQ=255)                                                                                                                                                   ccTCGCCACCGTTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCggggggg          <  1:301470/148‑1 (MQ=255)                                                                                                                                                        cAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGt  >  1:247874/1‑151 (MQ=255)                                                                                                                                                          |                                                                                                                                                     CATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAG‑TTTCCC‑TTAGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGT  >  NC_000913/3001015‑3001312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑