| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,880 | 0 | T | C | 60.5% | 16.3 / 41.7 | 38 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (9/6); new base C (0/23); total (9/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.07e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-06 | |||||||||||
ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCAGCT > NC_000913/357730‑358005 | aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTAttttt > 1:121400/1‑151 (MQ=255) cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccg < 2:762418/151‑1 (MQ=255) cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTcc < 2:711509/150‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca < 1:70383/150‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa < 1:824448/151‑1 (MQ=255) cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑ccgc > 2:328539/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTtctc > 1:376642/1‑151 (MQ=255) aTCAGGCAATCCCACCCGCAGCCCTGTGTCAGCGGCTACCGTGATTCATTCCCGCAAACACCCGCGCATTTACCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑ccgccg < 2:250807/145‑1 (MQ=18) aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTcc > 1:147777/1‑150 (MQ=255) aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCagt > 1:612930/1‑149 (MQ=255) ccGAAGACTTGTGTCGGCGGCTCCCGGATTTCTTTCCCCCCAACACCCGCGCATTTATCAAACGCCTTGTGAAAAATTG‑CCTTCGGGGGGGCTGTCTGCCGGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgg < 1:323213/149‑1 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCggggga > 1:232576/1‑147 (MQ=255) tgtgTCGGCGGCTCCCGTGATTATTTCCCGCAAACACCCGCGTTTTTACCCACCCCCATGTGAAAAAATC‑CCTTCGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAga‑ga < 1:477690/149‑1 (MQ=255) tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgggg > 2:878137/1‑130 (MQ=255) ttCTTTCCCCCCACCACCCGCGCTTTTACCCACCGCCATTGGAAAAAATC‑CCTTCGGGGCGGCTTTCCGCCAGTTTTTTTCCCGCCACAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCAt < 2:382259/149‑1 (MQ=255) ccagtcccGCAAACACCCGCGCTTTTAACCAACACATTGTAAAAATAGC‑CGTTCGTGG‑GGCTTTCCCCCGGTTTGTTTTCCCTCACAAAAAAATCCGCCGTTCTCTCCAGTAGTGTCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCAtgt < 2:292648/144‑1 (MQ=255) tcccccccAACACCCGCGCTTTTACCAACCCCCTTGTGAAAAAAGC‑CCTCCGGGGGGGCTGTCGCCCAGTTTTTTTCCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCAtgtt < 2:17681/142‑1 (MQ=255) ccccccttttACCCACCCCCATGTAAAAAAATC‑CTCCCGCGGCGGCTTCCTGCCAGTTTTTTTCCCGCCACAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgc < 2:202627/144‑1 (MQ=255) cgcgattttACCCACCGCAATGTAAAAAATTG‑CCTCCGGGGGGCCGGTCTGCCAGTTTTTTTCCCGCCACAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct < 2:859327/145‑1 (MQ=255) gtttttACCCAACGCCTTGTGCAAAATTG‑CCTTGGCGGCGGCTGTCTGCCGGTTATTTTCCCGCCCCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTgg < 1:799237/147‑1 (MQ=255) aTTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAACTTTAAAACCCCCATGTTTTGTGGGAATTgc > 1:346209/1‑142 (MQ=37) tttACCCACCCCCTTGTGAAAAAATC‑CCTCCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTaa < 1:228028/150‑1 (MQ=255) ccaccccaggttgaaaaaaGC‑CCCTCGGGGCGGCTGTCGGCCAGTTTTTTCCCCCCCAAAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AAtg < 2:308268/136‑1 (MQ=255) ccACCGCCATGGAAAAAATTC‑CCTCCGCGGCGGTTTTCAGCCTTTTTTTTTCCCCCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AAt < 2:396193/149‑1 (MQ=255) accGCCATGTGAAAAAATG‑CCTCCGGGGGGCCTGTCGCCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AAtgtg < 1:737834/148‑1 (MQ=255) accGCCATGTGAAAAAAGC‑CCTTCGGGGCGGTTTTCTCCCATTTTTTTTCCCCCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTgg < 1:959986/149‑1 (MQ=255) accGCCATGTAAAAAAATG‑CCTCCGGGGCGGCTGTCTGCCATTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTgg < 1:337629/149‑1 (MQ=255) aaccccatttaaaaaaTGCCCTGCGGGGGGGTTTTCGGCCAGTTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AAtgtg < 2:921701/139‑1 (MQ=255) cccttgtgAAAAATTG‑CCTTCGGGGCGGCTTTCTGCCAGCTATTTTCCCCCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGaa < 1:495312/146‑1 (MQ=255) ccATGTGCAAAAATG‑CCTTCGGGGGGGCTTTCGGCCAGCTATTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGaaa < 1:243422/150‑1 (MQ=255) aaaaaTG‑CCTTCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTg < 1:28125/150‑1 (MQ=255) aaaaaTG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGGAAAAAATTTTAAACCC‑TCCCGTTTTTTGGGAAAAGCTGCTGGGGACAAAATTGGAAAGGGagggg > 2:949599/1‑147 (MQ=255) aaaaaTG‑CCTGCGGGGCGGTTGTCTCCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTAGAAAGGGAAGTgc < 2:832318/151‑1 (MQ=255) cgcgGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGGAAAAACCTTTAAGCCC‑TCCTGTTGTGTTGGGATCGCTTCTGGTGAC‑AACATGGGAAGGGGAGGggggcggggt > 1:447839/1‑144 (MQ=255) gcggcgGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATc < 1:471768/147‑1 (MQ=255) gggggcTGTCACCCGGTTTTTTTCCCCCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCa < 1:810345/143‑1 (MQ=255) ggcgggTTTCTGCCATTTTTTTTCCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCa < 2:1250/146‑1 (MQ=255) ggcggcTGTCGGCCGTTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTATGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:973028/149‑1 (MQ=255) gggtttcctccAGCTATTTGCCCGCCGCAAAAAAT‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTgcggc < 1:70372/128‑1 (MQ=255) | ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCAGCT > NC_000913/357730‑358005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |