Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 366,526 | 0 | T | G | 47.1% | 70.8 / 18.9 | 51 | T329P (ACC→CCC) | lacI | lactose‑inducible lac operon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base T (5/22); new base G (24/0); total (29/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.69e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAG > NC_000913/366378‑366674 | aaGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGggtgg < 2:673956/151‑1 (MQ=255) gCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGtt < 1:781343/151‑1 (MQ=255) ccTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTTGGGGCCCGGGGGGgtt > 1:560157/1‑151 (MQ=255) aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTGAc < 2:792667/149‑1 (MQ=255) gagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGGGGGTTTTTTTTTCACCAGTg > 1:835584/1‑151 (MQ=255) gtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGAA‑TTGGGGCCCGGGGGGGTTTTTTTTTTTAccgag > 2:724822/1‑146 (MQ=255) gtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgag < 2:756629/151‑1 (MQ=255) agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GCC‑TTGGGGGCCCGGGGGGGTTTTTTTTTTACCacagag > 2:822410/1‑143 (MQ=255) agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgaga < 2:370959/149‑1 (MQ=255) cTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTG‑TTTGGGGCCAGGGGGGGTTTTTTTTTTTCCCGGGAGA‑Cggg > 1:291750/1‑151 (MQ=255) aaCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTATTTGGGGGCCGGGGGGGTTTTT‑TTTTTAACAGTGAGA‑Cggg > 1:517661/1‑149 (MQ=255) aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGAt < 1:619227/150‑1 (MQ=255) aTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTTGGGGGCCGGGGGGGTTTTCTTTTTTCCCGTGAGA‑AGGGcca > 2:586867/1‑139 (MQ=255) ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GGC‑TTGGGCGCCCCGGGGGTTTTTTTTTTTTCCCGTGagg‑caggccagacccggat > 2:647403/1‑133 (MQ=255) tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTc < 1:609781/150‑1 (MQ=255) tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTc < 1:646488/150‑1 (MQ=255) gcgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCa < 1:808898/150‑1 (MQ=255) cTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTT‑GCA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGAGAGA‑CGGGCAACAGCTGATTGTCCCTTAcccc > 1:909167/1‑149 (MQ=255) cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGcc < 1:322715/150‑1 (MQ=255) gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGc < 2:549575/150‑1 (MQ=255) gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGc < 2:355049/150‑1 (MQ=255) cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTg < 1:16100/151‑1 (MQ=255) gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GGT‑TTGGGGGCCCGGGGGGGTTTTTTTTTTACCCGTGAGA‑CGGGGGACAGCTGATTTCCCTTCCCCGCCTGGccc > 2:618332/1‑148 (MQ=255) ttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTTGGGGGCAGGGGGGGTTTTTTTTTTCCCAGAGGGA‑CGGGGAACAACTGATTTGCCTTTACCCCCCGGCCCCgag > 1:794644/1‑149 (MQ=255) ttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GGA‑TTGGGCGCCAGGGGGGGTTTTTTTTTTACCAGTGAGA‑CGGGCAACAGCCGGTTGGCCTTCACCGCCTGGccc > 2:944098/1‑145 (MQ=255) aGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GGA‑TTTGGCGGCCGGGGGGTTTTTTTTTTCACCCGCGAGG‑AGGGGAAAAAGTTATTGCCCTTTCCCGCCCGGccc > 2:91654/1‑141 (MQ=255) gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GGA‑GTTGGGGGCACGGGGGGTTTTCTTTTTACCAGGGGGA‑TGGGGAAAACCCGGTTGGCCCTCCCACCCTGGccccggcggcgat > 2:534790/1‑140 (MQ=255) aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCCCCCGGCCCTGAGAGAGGTgcagca > 2:677468/1‑150 (MQ=255) aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCCGGGTGGGTTTTTTTTTTACCAAGGAGA‑CGGGCAACAGCGGATTGCCCCTCACCCCCCGGCCCCGggagag > 2:854988/1‑142 (MQ=255) aCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGc < 2:819270/151‑1 (MQ=255) tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTCCCAGTGAGA‑CGGGCAACAGACCATTGCCCCTCACCCCCCGGCCCCGAGAGAGTTGCAGGAAACgg > 1:673141/1‑150 (MQ=255) tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GGA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTTCCCGGGAGA‑CGGGGAACAGCTGATTGGCCCTCCCCCCCCGGCCCCGCGAGAGGTGGAGCAAGCgg > 2:45577/1‑150 (MQ=255) gTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTATTTGGGGCCCAGGGGGGTTTTT‑TTTTTTCCAATGAGA‑CCGGCAAAAGCTGATTGCCCTTCACCGCCCGGCCCTGGGGGGGTTGCAGCAAACGGTcc > 1:500362/1‑149 (MQ=255) tGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGG‑GTA‑CTGTGCCCCCGGGGGGGTTTTTTTTTTTCCATAGAGA‑CGGGCCACAGCCGGTAGCCCTTTTCCCCCCGGCCCCTGGGGAGGTGCAGCAAGAGGGcc > 2:852692/1‑148 (MQ=255) ccAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt < 2:425551/151‑1 (MQ=255) aGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGA‑GGA‑TTGGGGGCCAGGGGGGGTTTTTTTTTTACCAGGGGGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTTGGCCTGGGAGAGTTGCAGCAAACGGTCCCCGCCgg > 1:87582/1‑151 (MQ=255) cATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTG‑GTA‑TTTGGGGCCACGGTGGGTTTTTTTTTTACCCGTGTGA‑AGGGCAACAGCTGATTTCCCTTCACCTCCCTGCCCCGAGGGAGTTGCAGCGAGAGGGcccccccgggtt > 1:776237/1‑139 (MQ=255) ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCCCCTGGCCCTGAGAGAGTTGCCGCAAGCGGGCCACCCCGGGTTGccc > 1:434935/1‑151 (MQ=255) gAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagc < 2:663782/150‑1 (MQ=255) aTCGGCCAACGCGCGGGGAGAGGCGGTTTTT‑GTA‑ATGGGGGCCAGGGGGGTTTTTTTTTTTCCCAAGGGGAGCGGGC‑CGAGCCCATTTGCCTTTACCGCCGGGGCCCGAGGGAGTTGCCGGAAGCGGTGCACGCTGGTTTTcccc > 2:224615/1‑145 (MQ=255) ggCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGa < 2:355097/151‑1 (MQ=255) ccAACGCGCGGGGAGAGGCGGTTTTC‑GCG‑TTGGGGGCCAGGGGGGGTTTTCTTTTCACCACCGGGA‑TGGGCAACACCGGATTTCCCTTCCCCCCCTGGCCCCGAGGGAGATGCAGCAAACGGTCAACGCCGGGTTGGCCCAGCAGGCGaa > 1:183886/1‑150 (MQ=255) cAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCCGGGGGGGTTTTTTTTTCACCAGGGGGA‑AGGGCAACAGCTGATTGGCCCTCCCCGCCCGGCCCTGGGGGAGGTGCAGCAAACGGGCCCCGCCGGGTTGGCCCCGCAgggggaa > 2:589224/1‑145 (MQ=255) cgGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGt < 2:322673/150‑1 (MQ=255) ggAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGa < 2:764778/151‑1 (MQ=255) gC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg < 1:224077/151‑1 (MQ=255) ttGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg > 1:127420/1‑150 (MQ=255) gggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGat < 1:132003/150‑1 (MQ=255) gcgcCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg > 1:741525/1‑146 (MQ=255) cgcCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGAtata > 2:826718/1‑150 (MQ=255) gggTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg < 2:813569/150‑1 (MQ=255) gggTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTAAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg < 1:348892/150‑1 (MQ=255) ggtggtTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg < 2:460801/151‑1 (MQ=255) ggtggtTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg < 2:417138/151‑1 (MQ=255) | AAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAG > NC_000913/366378‑366674 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |