Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913366,5260TG47.1% 70.8 / 18.9 51T329P (ACC→CCC) lacIlactose‑inducible lac operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (5/22);  new base G (24/0);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 8.69e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAG  >  NC_000913/366378‑366674
                                                                                                                                                      |                                                                                                                                                     
aaGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGggtgg                                                                                                                                                     <  2:673956/151‑1 (MQ=255)
  gCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGtt                                                                                                                                                   <  1:781343/151‑1 (MQ=255)
   ccTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGCCCGGGGGGgtt                                                                                                                                                  >  1:560157/1‑151 (MQ=255)
               aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTGAc                                                                                                                                        <  2:792667/149‑1 (MQ=255)
                  gagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGGGGGTTTTTTTTTCACCAGTg                                                                                                                                   >  1:835584/1‑151 (MQ=255)
                    gtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGAATTGGGGCCCGGGGGGGTTTTTTTTTTTAccgag                                                                                                                                    >  2:724822/1‑146 (MQ=255)
                    gtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgag                                                                                                                                 <  2:756629/151‑1 (MQ=255)
                       agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGCCTTGGGGGCCCGGGGGGGTTTTTTTTTTACCacagag                                                                                                                                 >  2:822410/1‑143 (MQ=255)
                       agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgaga                                                                                                                                <  2:370959/149‑1 (MQ=255)
                         cTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTGTTTGGGGCCAGGGGGGGTTTTTTTTTTTCCCGGGAGACggg                                                                                                                           >  1:291750/1‑151 (MQ=255)
                           aaCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGGCCGGGGGGGTTTTTTTTTTAACAGTGAGACggg                                                                                                                           >  1:517661/1‑149 (MQ=255)
                                     aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAt                                                                                                                <  1:619227/150‑1 (MQ=255)
                                      aTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGGCCGGGGGGGTTTTCTTTTTTCCCGTGAGAAGGGcca                                                                                                                        >  2:586867/1‑139 (MQ=255)
                                            ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGCTTGGGCGCCCCGGGGGTTTTTTTTTTTTCCCGTGaggcaggccagacccggat                                                                                                               >  2:647403/1‑133 (MQ=255)
                                             tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTc                                                                                                        <  1:609781/150‑1 (MQ=255)
                                             tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTc                                                                                                        <  1:646488/150‑1 (MQ=255)
                                              gcgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCa                                                                                                       <  1:808898/150‑1 (MQ=255)
                                                 cTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTTGCATTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGAGAGACGGGCAACAGCTGATTGTCCCTTAcccc                                                                                                   >  1:909167/1‑149 (MQ=255)
                                                   cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGcc                                                                                                  <  1:322715/150‑1 (MQ=255)
                                                       gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGc                                                                                              <  2:549575/150‑1 (MQ=255)
                                                       gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGc                                                                                              <  2:355049/150‑1 (MQ=255)
                                                          cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTg                                                                                          <  1:16100/151‑1 (MQ=255)
                                                           gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGTTTGGGGGCCCGGGGGGGTTTTTTTTTTACCCGTGAGACGGGGGACAGCTGATTTCCCTTCCCCGCCTGGccc                                                                                            >  2:618332/1‑148 (MQ=255)
                                                              ttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGGCAGGGGGGGTTTTTTTTTTCCCAGAGGGACGGGGAACAACTGATTTGCCTTTACCCCCCGGCCCCgag                                                                                        >  1:794644/1‑149 (MQ=255)
                                                              ttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCCGGTTGGCCTTCACCGCCTGGccc                                                                                            >  2:944098/1‑145 (MQ=255)
                                                                  aGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGATTTGGCGGCCGGGGGGTTTTTTTTTTCACCCGCGAGGAGGGGAAAAAGTTATTGCCCTTTCCCGCCCGGccc                                                                                            >  2:91654/1‑141 (MQ=255)
                                                                   gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGAGTTGGGGGCACGGGGGGTTTTCTTTTTACCAGGGGGATGGGGAAAACCCGGTTGGCCCTCCCACCCTGGccccggcggcgat                                                                                  >  2:534790/1‑140 (MQ=255)
                                                                         aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGCCAGGGGGGGTTTTTTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCCGGCCCTGAGAGAGGTgcagca                                                                            >  2:677468/1‑150 (MQ=255)
                                                                         aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCCGGGTGGGTTTTTTTTTTACCAAGGAGACGGGCAACAGCGGATTGCCCCTCACCCCCCGGCCCCGggagag                                                                                    >  2:854988/1‑142 (MQ=255)
                                                                           aCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGc                                                                         <  2:819270/151‑1 (MQ=255)
                                                                              tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCCGGGGGGGTTTTTTTTTTCCCAGTGAGACGGGCAACAGACCATTGCCCCTCACCCCCCGGCCCCGAGAGAGTTGCAGGAAACgg                                                                       >  1:673141/1‑150 (MQ=255)
                                                                              tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGGGCCCGGGGGGGTTTTTTTTTTTCCCGGGAGACGGGGAACAGCTGATTGGCCCTCCCCCCCCGGCCCCGCGAGAGGTGGAGCAAGCgg                                                                       >  2:45577/1‑150 (MQ=255)
                                                                                  gTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGGGCCCAGGGGGGTTTTTTTTTTTCCAATGAGACCGGCAAAAGCTGATTGCCCTTCACCGCCCGGCCCTGGGGGGGTTGCAGCAAACGGTcc                                                                    >  1:500362/1‑149 (MQ=255)
                                                                                   tGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGGGTACTGTGCCCCCGGGGGGGTTTTTTTTTTTCCATAGAGACGGGCCACAGCCGGTAGCCCTTTTCCCCCCGGCCCCTGGGGAGGTGCAGCAAGAGGGcc                                                                    >  2:852692/1‑148 (MQ=255)
                                                                                     ccAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt                                                               <  2:425551/151‑1 (MQ=255)
                                                                                       aGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGAGGATTGGGGGCCAGGGGGGGTTTTTTTTTTACCAGGGGGACGGGCAACAGCTGATTGCCCTTCACCGCCTTGGCCTGGGAGAGTTGCAGCAAACGGTCCCCGCCgg                                                             >  1:87582/1‑151 (MQ=255)
                                                                                            cATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTGGTATTTGGGGCCACGGTGGGTTTTTTTTTTACCCGTGTGAAGGGCAACAGCTGATTTCCCTTCACCTCCCTGCCCCGAGGGAGTTGCAGCGAGAGGGcccccccgggtt                                                          >  1:776237/1‑139 (MQ=255)
                                                                                              ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGGGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCTGGCCCTGAGAGAGTTGCCGCAAGCGGGCCACCCCGGGTTGccc                                                      >  1:434935/1‑151 (MQ=255)
                                                                                                   gAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagc                                                  <  2:663782/150‑1 (MQ=255)
                                                                                                     aTCGGCCAACGCGCGGGGAGAGGCGGTTTTTGTAATGGGGGCCAGGGGGGTTTTTTTTTTTCCCAAGGGGAGCGGGCCGAGCCCATTTGCCTTTACCGCCGGGGCCCGAGGGAGTTGCCGGAAGCGGTGCACGCTGGTTTTcccc                                                     >  2:224615/1‑145 (MQ=255)
                                                                                                        ggCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGa                                            <  2:355097/151‑1 (MQ=255)
                                                                                                          ccAACGCGCGGGGAGAGGCGGTTTTCGCGTTGGGGGCCAGGGGGGGTTTTCTTTTCACCACCGGGATGGGCAACACCGGATTTCCCTTCCCCCCCTGGCCCCGAGGGAGATGCAGCAAACGGTCAACGCCGGGTTGGCCCAGCAGGCGaa                                           >  1:183886/1‑150 (MQ=255)
                                                                                                           cAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCCGGGGGGGTTTTTTTTTCACCAGGGGGAAGGGCAACAGCTGATTGGCCCTCCCCGCCCGGCCCTGGGGGAGGTGCAGCAAACGGGCCCCGCCGGGTTGGCCCCGCAgggggaa                                          >  2:589224/1‑145 (MQ=255)
                                                                                                                  cgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGt                                   <  2:322673/150‑1 (MQ=255)
                                                                                                                     ggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGa                               <  2:764778/151‑1 (MQ=255)
                                                                                                                                  gCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg                  <  1:224077/151‑1 (MQ=255)
                                                                                                                                         ttGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg              >  1:127420/1‑150 (MQ=255)
                                                                                                                                           gggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGat            <  1:132003/150‑1 (MQ=255)
                                                                                                                                             gcgcCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg              >  1:741525/1‑146 (MQ=255)
                                                                                                                                              cgcCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGAtata         >  2:826718/1‑150 (MQ=255)
                                                                                                                                                   gggTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg    <  2:813569/150‑1 (MQ=255)
                                                                                                                                                   gggTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTAAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg    <  1:348892/150‑1 (MQ=255)
                                                                                                                                                    ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg  <  2:460801/151‑1 (MQ=255)
                                                                                                                                                    ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg  <  2:417138/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAG  >  NC_000913/366378‑366674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: