Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,764,096 A→T I353N (ATC→AAC)  yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,764,0960AT100.0% 263.5 / NA 66I353N (ATC→AAC) yfjLCP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (34/32);  total (34/32)

CACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCAT  >  NC_000913/2763954‑2764240
                                                                                                                                              |                                                                                                                                                
cACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCg                                                                                                                                          >  2:80966/1‑151 (MQ=255)
    aaCTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGc                                                                                                                                       >  1:201939/1‑150 (MQ=255)
                 cAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGAcc                                                                                                                         >  1:455927/1‑151 (MQ=255)
                       aaaGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCaaaa                                                                                                                    >  2:683361/1‑150 (MQ=255)
                          gCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTc                                                                                                                >  2:642654/1‑151 (MQ=255)
                          gCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTc                                                                                                                <  1:632743/151‑1 (MQ=255)
                             gATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCg                                                                                                               <  1:702708/149‑1 (MQ=255)
                              aTCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTg                                                                                                            <  1:127391/151‑1 (MQ=255)
                               tCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCg                                                                                                               <  2:615319/147‑1 (MQ=255)
                                cACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGAc                                                                                                          >  2:690074/1‑151 (MQ=255)
                                 aCCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACg                                                                                                         <  2:141077/151‑1 (MQ=255)
                                    ttCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTc                                                                                                       >  2:135412/1‑150 (MQ=255)
                                        ggATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATgg                                                                                                   >  1:714028/1‑150 (MQ=255)
                                         gATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATgg                                                                                                   >  2:823/1‑149 (MQ=255)
                                            cgcgGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCg                                                                                               <  2:866643/150‑1 (MQ=255)
                                             gcggcgAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGAt                                                                                             >  1:431945/1‑151 (MQ=255)
                                               ggcgAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGAttt                                                                                           >  2:957657/1‑151 (MQ=255)
                                                 cgAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTg                                                                                          >  1:327649/1‑150 (MQ=255)
                                                    aCGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGa                                                                                      <  1:834522/151‑1 (MQ=255)
                                                        tatGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACgg                                                                                   >  1:600641/1‑150 (MQ=255)
                                                          tGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAAt                                                                                >  2:29878/1‑151 (MQ=255)
                                                           gCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAAt                                                                                <  2:408171/150‑1 (MQ=255)
                                                              cGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTTCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCTTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGTCGGAATTCtt                                                                            >  1:372817/1‑151 (MQ=255)
                                                              cGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCtt                                                                            >  1:88288/1‑151 (MQ=255)
                                                              cGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCtt                                                                            <  1:167848/151‑1 (MQ=255)
                                                               gTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCttt                                                                           >  2:455275/1‑151 (MQ=255)
                                                                ttttCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCttt                                                                           >  2:966564/1‑150 (MQ=255)
                                                                   tCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTc                                                                       >  1:711393/1‑151 (MQ=255)
                                                                    cAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCg                                                                      <  2:119117/151‑1 (MQ=255)
                                                                       gAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTtc                                                                   <  2:432611/151‑1 (MQ=255)
                                                                         aTTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTtctc                                                                 >  1:351972/1‑151 (MQ=255)
                                                                             tttCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCt                                                              <  1:399171/150‑1 (MQ=255)
                                                                              ttCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCtt                                                             <  1:80966/150‑1 (MQ=255)
                                                                               tCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCttt                                                            <  2:872986/150‑1 (MQ=255)
                                                                                cGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCtttt                                                           <  2:593373/150‑1 (MQ=255)
                                                                                   ccccTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCaat                                                       <  1:347542/151‑1 (MQ=255)
                                                                                    cccTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCaat                                                       >  1:171155/1‑150 (MQ=255)
                                                                                     ccTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCaatga                                                     <  1:314290/151‑1 (MQ=255)
                                                                                     ccTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCaatga                                                     <  2:502180/151‑1 (MQ=255)
                                                                                        gCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCaatgaatg                                                  <  1:308246/151‑1 (MQ=255)
                                                                                         cTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCATTGAATGt                                                 >  1:802274/1‑151 (MQ=255)
                                                                                             ttCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAg                                              <  1:406335/150‑1 (MQ=255)
                                                                                               ccACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGttt                                           >  1:183210/1‑151 (MQ=255)
                                                                                                 aCGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTAt                                         <  1:825856/151‑1 (MQ=255)
                                                                                                   gTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATg                                        >  2:532410/1‑150 (MQ=255)
                                                                                                   gTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGa                                       <  1:285009/151‑1 (MQ=255)
                                                                                                       aGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAata                                    <  1:190405/150‑1 (MQ=255)
                                                                                                          aTGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAg                                <  1:976242/151‑1 (MQ=255)
                                                                                                            gCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGa                               <  2:872559/150‑1 (MQ=255)
                                                                                                                  aaTAGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCt                        >  1:157131/1‑151 (MQ=255)
                                                                                                                     aGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTTACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCttt                      <  2:432391/150‑1 (MQ=255)
                                                                                                                     aGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCtttt                     <  2:417864/151‑1 (MQ=255)
                                                                                                                     aGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCtttt                     >  2:884856/1‑151 (MQ=255)
                                                                                                                     aGGCTGGCTTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCttt                      <  2:746828/150‑1 (MQ=255)
                                                                                                                       gctggctTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTgg                   <  2:711390/151‑1 (MQ=255)
                                                                                                                       gctggctTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTgg                   >  1:580190/1‑151 (MQ=255)
                                                                                                                          ggctTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCg                <  1:747418/151‑1 (MQ=255)
                                                                                                                           gctTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCgg               >  1:793062/1‑151 (MQ=255)
                                                                                                                            ctTTCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCgg               >  1:781035/1‑150 (MQ=255)
                                                                                                                              ttCGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGga              <  2:586995/149‑1 (MQ=255)
                                                                                                                                cGTGGTGCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGaa           <  1:600176/150‑1 (MQ=255)
                                                                                                                                 gtggtgCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGaaa          >  2:66572/1‑150 (MQ=255)
                                                                                                                                 gtggtgCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGaaa          >  1:453741/1‑150 (MQ=255)
                                                                                                                                  tggtgCTGCCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGaaaa         >  2:728554/1‑150 (MQ=255)
                                                                                                                                       ctgcCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTc    >  2:439859/1‑150 (MQ=255)
                                                                                                                                        tgcCGGTTCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCAt  >  2:330974/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCAT  >  NC_000913/2763954‑2764240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: