Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,000,4210AT42.9% 43.4 / 13.6 36Y26F (TAT→TTT) xdhAxanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base A (7/13);  new base T (15/0);  total (23/13)
Fisher's exact test for biased strand distribution p-value = 5.87e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGCCTGATATTCCATTCGCTACAGCTGGGCATGCCTGGAC  >  NC_000913/3000280‑3000568
                                                                                                                                             |                                                                                                                                                     
cAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGGAGGTGGTTTGCGgaa                                                                                                                                                >  2:26378/1‑149 (MQ=255)
cAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGGGTTTTTCGaaa                                                                                                                                                >  1:565192/1‑149 (MQ=255)
  aaTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATg                                                                                                                                            <  1:460522/151‑1 (MQ=255)
     gTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTAc                                                                                                                                         <  1:3518/151‑1 (MQ=255)
       tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGGAAAATGTACg                                                                                                                                        >  1:263167/1‑150 (MQ=255)
          aaTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGc                                                                                                                                    <  2:40675/151‑1 (MQ=255)
           aTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCGTGTGGTTTGCGAAATAAGTACGTa                                                                                                                                      >  1:232866/1‑148 (MQ=255)
            tCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTTTTCGAAAAATGTACGTAGccc                                                                                                                                  >  1:624469/1‑151 (MQ=255)
                ggggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGGATGTGGTGTGCGGAAAATGTACGGAAGCCTAt                                                                                                                               >  2:206385/1‑150 (MQ=255)
                ggggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATc                                                                                                                              <  1:760801/151‑1 (MQ=255)
                 gggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCg                                                                                                                             <  2:111011/151‑1 (MQ=255)
                     tttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGGTTTGCGGAATATGTAAGGAACCCCATCGcac                                                                                                                          >  1:677705/1‑150 (MQ=255)
                       tCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTTTTCGAAAAAAGTAAGTAGCCCCATccccc                                                                                                                          >  1:142196/1‑144 (MQ=255)
                         tATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGGATGTGGTTTGCGAAATATGttaggaaccc                                                                                                                                  >  2:212658/1‑129 (MQ=255)
                          aTGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATgg                                                                                                                     <  1:397731/149‑1 (MQ=255)
                             gAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATg                                                                                                                <  1:567561/151‑1 (MQ=255)
                                             ccGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatga                                                                                                <  1:167012/151‑1 (MQ=255)
                                                cTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGGATGTGTTTTTCGAAAAATGTAAGGAGCCCCATCGCACAAGGGTTTGGCGGAAGAATTAat                                                                                                  >  1:474908/1‑146 (MQ=255)
                                                    aggTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTTTTCGGAAAATAGACGCAGCCCCATTCCACCAGGTTTTGCCGTAAGAATTAATgaagaaaa                                                                                          >  1:83628/2‑148 (MQ=255)
                                                         aaTCATGCATGCGCGTCGATGCCATTGCAAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTATTATGCGAAATATGTACGTAGCCCTACCGCACATGGTTATGCCGTAAGTATTAATGATGATCAAGc                                                                                       <  2:236080/148‑1 (MQ=255)
                                                           tCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGGGTTTGTTTTGCGAAAAATATAAGTAGACCCATTGCCCCTGGTTTTTTCGGAAAAATTAATTATGAACaa                                                                                         >  2:653597/1‑144 (MQ=255)
                                                             atgcatgcGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTTTGCGAAATATTTATGTAGCCCCATCGCACACGGGTTTTTCGTAAGGATTAATTATGAAAAAACCAGa                                                                                   >  1:534247/1‑148 (MQ=255)
                                                                   gcgcGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGGCTTGGTTATGACGAATCTTAAAGTTGCCCTATTCCACACGGTTTAGCCGTAAAGATTAATTATGAACAAAACACAAGTTTGcc                                                                           >  1:718617/1‑150 (MQ=255)
                                                                   gcgcGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCg                                                                          <  2:116189/151‑1 (MQ=255)
                                                                      cgTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGc                                                                       <  2:454439/151‑1 (MQ=255)
                                                                       gTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAAGTGTTTTGCGAAAAATGTACGTAGCCCCATCTCACCTGGTTTTGCCGTAAGGATTTATGATGAACAAAACCGAAGTTTTCCGGGCg                                                                      >  1:593696/1‑151 (MQ=255)
                                                                                     tAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGGAAGTGTTTTGCGAAATATGTAAGTAGCCCTATCGCAAAAGGTTATTGCGTAAGGATTAATGATGAACAAAACAGAAATTTTCCGGGGGGAATGGCGGttttt                                                       >  1:50319/1‑151 (MQ=255)
                                                                                                      cACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATACGGAAAAATTACGTTGCCCTTTCCCACATGGGTTTGGCCGAAAGATTAATGATGAACAAACCCGAAGGTTGACGGGCGTACTGGGGCTTTTTTTCTGGGAAGGtgtg                                        >  1:723284/1‑149 (MQ=255)
                                                                                                           tataCTGACGATTATGTTATGGCGGGGATGGGTTATGCGGAATATATAAGTAGCCCCATTGCCCAAGGTTATGGCGTAAGGATTAATTATTAAAAAAACAGAAGATTTCCGGGCGGACTGGCGGTTTTTTTCTGGGgagaagggtctg                                    >  2:711735/1‑140 (MQ=255)
                                                                                                              aCTGACGATTATGTTATGGCGGGCCTGTGGTTTTCGAAAAATGTAACTAACCCTATACCACAAGGGTTTTGCGGAAAAATTAATTATGAAAAAGCCAGAAGTTTGCCCGGCGTACTGGCGGGTTTTTCCCTGGGAAAAGGGTCTGATAtt                               >  1:140118/1‑150 (MQ=255)
                                                                                                                        atgttatgGCGGGCATGTGTTTTGCGAAAAATTTAAGTAACCCTTTTACACATGGGTTTGCCGGAAAGATTTAAAATGAACAAACCCGAAGGTTTGCGGGGGGACTGGCGGTTTTTTTCTTGGGAGAAGTGGCTGGTAtttcctt                          >  2:183599/1‑140 (MQ=255)
                                                                                                                              tgGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGCCTGATATTCCATTCGCTACAGCTgg              >  2:613247/1‑151 (MQ=255)
                                                                                                                                  gggCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGCCTGATATTCCATTCGCTACAGCTGGGCAt          <  1:577576/151‑1 (MQ=255)
                                                                                                                                  gggCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGCCTGATATTCCATTCGCTACAGCTGGGCAt          >  1:442319/1‑151 (MQ=255)
                                                                                                                                      aTGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGCCTGATATTCCATTCGCTACAGCTGGGCATGCCt      >  1:39476/1‑151 (MQ=255)
                                                                                                                                          gtTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGCCTGATATTCCATTCGCTACAGCTGGGCATGCCTGGac  <  1:743835/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
CAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGCCTGATATTCCATTCGCTACAGCTGGGCATGCCTGGAC  >  NC_000913/3000280‑3000568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: