Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT55.3% 12.2 / 55.4 38intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (3/14);  new base T (21/0);  total (24/14)
Fisher's exact test for biased strand distribution p-value = 7.03e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-07

TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTA  >  NC_000913/3206293‑3206573
                                                                                                                                              |                                                                                                                                           
ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaa                                                                                                                                      <  2:156664/150‑1 (MQ=255)
     aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTcaaacaaaaa                                                                                                                                   >  2:287591/1‑146 (MQ=255)
        aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                             <  1:560491/151‑1 (MQ=255)
                 ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTAGAcaaaaacaaaaaaatgaggt                                                                                                                     >  2:255627/1‑142 (MQ=255)
                  tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAAAAAAAACAgggg                                                                                                                       >  2:406240/1‑145 (MQ=255)
                   cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGGATTTTTTTAGAAAAATAGAAAAACTTGAg                                                                                                                      >  1:359900/1‑146 (MQ=255)
                      tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAggt                                                                                                                    >  1:452583/1‑143 (MQ=255)
                        ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGTTGcc                                                                                                                 >  1:759291/1‑146 (MQ=255)
                               gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCTCAAAAAAAAAAAAACATGGGCTTCCATGATGAgg                                                                                                        >  2:562527/1‑147 (MQ=255)
                               gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaa                                                                                                     <  2:653874/151‑1 (MQ=255)
                                 aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCCACAaaaaaaaaaaaaaggggttcc                                                                                                                  >  1:140255/1‑126 (MQ=255)
                                 aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGAGGTGTCATGATGGGCg                                                                                                       >  2:16087/1‑147 (MQ=255)
                                 aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                   <  1:171831/151‑1 (MQ=255)
                                 aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                   <  1:412913/151‑1 (MQ=255)
                                    attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                 <  2:459424/150‑1 (MQ=255)
                                       cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata                                                                                              <  1:738977/150‑1 (MQ=255)
                                        attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAACAAAAAAAAAAATGGAGGTTCCATGATGAGGGGAAGaaaaaa                                                                                             >  2:607376/1‑150 (MQ=255)
                                             tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAATAAAAAATGGAGTTGCCATGATGAGGGGAAGgaaaaaaaaa                                                                                         >  1:372144/1‑145 (MQ=255)
                                                   cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg                                                                                  <  1:390622/150‑1 (MQ=255)
                                                    gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAAAAAAAAAAAAAAATGGGGTTGCCTTGATTGGGGAAAGAAAAAAAAAAAAGGCAgg                                                                                 >  1:313068/1‑149 (MQ=255)
                                                               cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAGACAAATAAAAAAAATGGAGGTGTCATGATGAGGGGAAGGAAAAAAAAAAAGGCAGGGAAAAAGtt                                                                        >  2:215099/1‑148 (MQ=255)
                                                                  aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga                                                                  <  1:643606/151‑1 (MQ=255)
                                                                     gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                 <  2:53875/149‑1 (MQ=255)
                                                                      ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATTGAGGTGGCATGATTGGGGGAAGTAATAAACAAAAAGCAGTGAATAAATTGACa                                                                    >  2:514694/1‑145 (MQ=255)
                                                                             cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAATGGAGTTGCCCTTATGAGCGAAAGTAATAAACAACAGGCAGGGAATAAATTGACAGAGATTGt                                                            >  1:188970/1‑146 (MQ=255)
                                                                              atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATTGAGTTGCCCTGATGAGGGGAAGGAATAAACAACAAGGAGTGAATAAATTGACAGAGATTGt                                                            >  1:324795/1‑145 (MQ=255)
                                                                                  tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                   <  1:536045/150‑1 (MQ=255)
                                                                                   cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                 <  2:183301/151‑1 (MQ=255)
                                                                                      ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGACAAAAAAAAAAAATGGAGGTGCCCTGATTAGCGGAAAAAATAAACAACCAGGAGGGGATAAGGTGACAGAGATTTTCGCTAACttt                                                  >  1:748221/1‑147 (MQ=255)
                                                                                            aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAAAAAAAAAAAGAGGTGCCATGATGAGCGAAAGTAAAAAACAAAAAGGAGTGAATAAAGTGACAGAGATTGTTGCTAAATTTACcccc                                            >  1:85026/1‑147 (MQ=255)
                                                                                                     aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTTGCCCGATGAGCGGAAGAAAAAAGCAACAGGGAGGGAATAAAATTGCAGAGAATGTTGCTAAATTTACCCCCACGATTTTTAccc                               >  1:321410/1‑151 (MQ=255)
                                                                                                                   aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGGGCTGCCCTGATGGGCGGAAATAATAAGAAAAAGGGCGAGAAAAAGTTGACAGAGGATGTCGCCAAACTTATCGCCATGGTTTTTACTCCAATACCtggtga                  >  1:765381/1‑150 (MQ=255)
                                                                                                                   aGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAAGAATAAAAAAAAAGGAGGGAAAAAAATGACAGAGAATGTCGCTAACTTTTCCGCCATTATTTTTTCCCGAAAGGCtg                      >  2:731815/1‑146 (MQ=255)
                                                                                                                              gtgGCGGTAAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCTTGATGAGCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa         <  2:424938/148‑1 (MQ=255)
                                                                                                                                ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa     >  2:629764/1‑150 (MQ=255)
                                                                                                                                ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc    >  2:233932/1‑151 (MQ=255)
                                                                                                                                ggCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAGGTTGACAAAGATTGTCGCTAACTATACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc    <  1:756094/151‑1 (MQ=255)
                                                                                                                                   ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa  >  1:589068/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTA  >  NC_000913/3206293‑3206573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: