Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,743,657	0	A	C	43.1%	77.2 / 16.1	51	T309P (ACC→CCC)	yiaK	2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (22/7);  new base C (0/22);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.06e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCT  >  NC_000913/3743509‑3743807                                                                                                                                                       |                                                                                                                                                       ttCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTtactac                                                                                                                                                       <  2:688013/150‑1 (MQ=255) ttCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTtactac                                                                                                                                                       <  1:730469/150‑1 (MQ=255)    acaAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCctg                                                                                                                                                   >  2:15067/1‑151 (MQ=255)       aaTTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCctgctg                                                                                                                                                <  1:59383/151‑1 (MQ=255)          ttttATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGc                                                                                                                                              <  1:722801/150‑1 (MQ=255)            ttATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCg                                                                                                                                            <  2:710649/150‑1 (MQ=255)             tATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGa                                                                                                                                           <  1:686283/150‑1 (MQ=255)               ttGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGaaa                                                                                                                                         >  2:462812/1‑150 (MQ=255)                tGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAAc                                                                                                                                       >  1:431363/1‑151 (MQ=255)                 gCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAAcc                                                                                                                                      <  2:101213/151‑1 (MQ=255)                   cATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAAccgc                                                                                                                                    >  2:79356/1‑151 (MQ=255)                    aTTGAAGTGGTCAAGATTATCGATGGTCCCACGCGCGATGCGAAGCTGCAAAGCATCATGGATGACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCTGTT‑CGCATAACAGGCGATGAA‑GATACTACCCTGATGGCGGAAAAccgc                                                                                                                                    >  2:214432/1‑150 (MQ=255)                    aTTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAAccgcc                                                                                                                                   >  2:380847/1‑151 (MQ=255)                       gAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGt                                                                                                                                 >  2:342300/1‑150 (MQ=255)                                    ttACCGACGTCCCCCCCCGCGACGCAAGGCTAAAACGCATCATGGTTTACTTTCCAAGTCCGGGGCGTGCGGACAAAAACCAGGCCATC‑CCCTCCCCCGGCAATAAA‑TTTCCTCCCCTGCTGCCCAAAAACCGCCGTAACGGCATCACt                                                                                                                     <  1:335650/149‑1 (MQ=255)                                       tCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTtg                                                                                                                 >  2:188302/1‑150 (MQ=255)                                            ggcccccccCGCGATGCAAGGCGGAACCGCATCATGAATTCCTTTCCTAGGGCGGGGGGTGCTGACAAAAACCGGGCCTTC‑CGCTCCCCCGGCAATAAT‑TTTCCTCCCCTGCTGGCCAAAAACCGCCGTAACGGCATCACTGTtgatga                                                                                                             <  1:309819/146‑1 (MQ=255)                                               ccccccCGCGATGCCAAGCTCCAACGCATCGTGTTTTCCTTTATTAGGGCCGGGCGTCTTGAAAAAAACCAGCCCATC‑CCCTTCCCCGCCCATAAT‑TTTCCTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAg                                                                                                          <  1:83327/149‑1 (MQ=255)                                               ccccccCGCGATGCAAACCTGCAACGCATCAGGGATTACTTTACTAGGGCGGGGCGTGCTGAAAAAAACCAGGCCATT‑CGCTTCCCCGGCCATAAA‑TTTACTCCCCTGCGGGCGAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCg                                                                                                        <  1:233252/151‑1 (MQ=255)                                               ccccccCGCGAGGCAAAGCGGAAACGCATCATGGTTTACTTTACTAGGGCGGGGCGTGCTAAC‑AAAAACCGGCCTTTCCGCTCCCCCGCCAATAAT‑TTTACTCCCCGCCGGGCGGAAAACCGCCGTAACGGCATCACTGTTGATGACAGc                                                                                                         <  2:225842/150‑1 (MQ=255)                                                 cccccGCGATGCCAAGCTGAAACGCATCTGGGTTTACGTTATGAGTGCCGGGGGTGCTGAAAAAAACCGGCCCATC‑CGCTTACCCGGCCATAAT‑TTTACTACCCTGGTGGCAGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCgtg                                                                                                      <  2:579191/149‑1 (MQ=255)                                                  ccccGCGATGCCAAGCTAAAACGCATCATGGATTTCTTTACTAGGGCGGGGGGTGCTGAAAAAAACCGGCCCATT‑CGCTTCCCCGCCCATAAA‑TTTACTCCCCTGCGGGCGAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCgtgt                                                                                                     <  1:67371/150‑1 (MQ=255)                                                      gcgATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGc                                                                                                 >  1:130379/1‑151 (MQ=255)                                                          tGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCaa                                                                                              >  1:235470/1‑150 (MQ=255)                                                            ccAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAAt                                                                                           >  1:194137/1‑151 (MQ=255)                                                             cAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATc                                                                                          >  1:387520/1‑151 (MQ=255)                                                                  tGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGc                                                                                     >  1:437182/1‑151 (MQ=255)                                                                     accccgccatGTATTCTGTTACTGGGCCGGAGCGTCGTAACAAAAACCGGGCCTTT‑CCCTTCCCCGGCCATGAT‑TTCATTCCCCCGGTGGCGAAACCCCGCCGTAACGGCATCACTGTTGATGACAGCGTGTggg                                                                                                  <  2:627618/128‑1 (MQ=255)                                                                     accccataatGTATTACGTTATTGGTGCGGGGGGTCCTGAAAAAAACCGGCCCTTC‑CGCTTCCCCGGCCTTAAT‑TTTCCCCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCa                                                                                        <  1:342302/141‑1 (MQ=255)                                                                      aCGCATCATGGATTGCTTTACGGGGCCCGAGCGTCCGGACAAAAATCAGGCCTTC‑CGCTTACCCGCCAATAAT‑TTTACTACCCTGCGGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTa                                                                                 <  2:481028/151‑1 (MQ=255)                                                                              tgtaTTACTTTACTAGTGCGGGGCGTGCTGACAAAAATCAGGCCATT‑CGCTTCCCCGGCAATAAA‑TTTCCTCCCCTGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgagg                                                                            <  1:25567/145‑1 (MQ=255)                                                                               ggATTACTTTTCTAGTGCGGGGGGTGCTGACAAAATTCGGGCCTTC‑CCCTTCCCCGGCAATGAT‑TTTCCCCCCCTGCTGGCGGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGgaga                                                                         <  2:686554/150‑1 (MQ=255)                                                                                 aTTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATa                                                                       >  1:487443/1‑150 (MQ=255)                                                                                  ttactttactAGGGCCGAGCGTGCTGACAAAACACA‑GCCCTT‑CCCCTACCCCGCCCAGAATTTTCCTCCCCGGCTGGCAAAAACCCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATaa                                                                      <  1:177616/150‑1 (MQ=255)                                                                                     cGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTc                                                                   >  1:126402/1‑150 (MQ=255)                                                                                        tccTAGTGCCGGGCGTGCTGACAAAAACCAGCCCATT‑CCCTTCCCCGGCCATAAT‑TTTACTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCAt                                                                 <  1:386012/147‑1 (MQ=255)                                                                                                  gAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGAc                                                      >  1:627869/1‑150 (MQ=255)                                                                                                  gAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGAc                                                      >  1:368422/1‑150 (MQ=255)                                                                                                   aGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACat                                                    >  1:185348/1‑151 (MQ=255)                                                                                                            aaaaaaaTCAGCCCTTT‑CCCTTCCCCGGCCATAAT‑TTTACTCCCCTGCTGGCGAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCa                                            <  1:637410/147‑1 (MQ=255)                                                                                                              gAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGcc                                         >  1:656929/1‑151 (MQ=255)                                                                                                               aaaaCCAGCCCTTT‑CGTTTCCCCGGCCATGAT‑TTTCCTCCCCTGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGcc                                         <  2:575130/150‑1 (MQ=255)                                                                                                                   tCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATc                                    >  2:58988/1‑151 (MQ=255)                                                                                                                      gcccATT‑CGCTCCCCCGGCAATAAA‑TTTACTCCCCGGCGGCCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAAt                                     <  1:607753/145‑1 (MQ=255)                                                                                                                         ccttccGCTTCCCGGGCAATAAT‑TTTACTCCCCGGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGc                               <  2:433341/146‑1 (MQ=255)                                                                                                                                cTTCCCGGGCAATAAA‑TTTCCTCCCCTGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTg                         <  1:567466/150‑1 (MQ=255)                                                                                                                                cTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGc                        >  2:235323/1‑151 (MQ=255)                                                                                                                                    cccGGCCTTGTA‑TTTACCCCCCTGCTGCCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGc                        <  1:690174/147‑1 (MQ=255)                                                                                                                                    cccGGCAATGAA‑TTTCCTCCCCTGCTGGCGAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGccc                      <  1:528915/149‑1 (MQ=255)                                                                                                                                    cccGGCAATAAT‑TTTCCCCCCCTGCTGGCAAAAAGCCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGttt                          <  2:711293/145‑1 (MQ=255)                                                                                                                                    cccGCCAATAAA‑TTTCCTCCCCGGGTGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGttt                          <  2:720910/145‑1 (MQ=255)                                                                                                                                         ccATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCgccgcc                >  1:722558/1‑150 (MQ=255)                                                                                                                                            agaattttCCTCCCCGGGTGGCCGAAACCCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCgccgcc                <  1:354791/143‑1 (MQ=255)                                                                                                                                                   tactacCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAgg      >  1:603495/1‑151 (MQ=255)                                                                                                                                                     ctacCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGgcg    >  2:165727/1‑151 (MQ=255)                                                                                                                                                     ctacCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGgcg    >  1:490712/1‑151 (MQ=255)                                                                                                                                                       acCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCt  >  2:555149/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                       TTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATT‑CGCTTACCCGGCCATGAA‑TTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCT  >  NC_000913/3743509‑3743807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑