Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,282	0	T	G	54.4%	48.4 / 68.9	46	V526G (GTT→GGT)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/20);  new base G (25/0);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 3.74e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-08

TGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354136‑354430                                                                                                                                                   |                                                                                                                                                     tGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTgg                                                                                                                                                   <  1:408543/150‑1 (MQ=255)  gACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTggg                                                                                                                                                  <  2:253697/150‑1 (MQ=255)   aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTgggg                                                                                                                                                 >  1:396906/1‑150 (MQ=255)      ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTg                                                                                                                                               <  1:507963/149‑1 (MQ=255)             ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgc                                                                                                                                       <  2:148317/150‑1 (MQ=255)                      tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAggg                                                                                                                             >  1:763163/1‑151 (MQ=255)                          ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAgg                                                                                                                         <  2:750611/151‑1 (MQ=255)                                  cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGCGGGTGGGGGGCAAGATGGGCTGAAAGGGCGgggggcggg                                                                                                                  >  1:422298/1‑149 (MQ=255)                                     tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                               <  2:63910/150‑1 (MQ=255)                                     tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                               <  1:442955/150‑1 (MQ=255)                                      aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAGGGGCggtgggggggg                                                                                                                >  1:65973/1‑148 (MQ=255)                                      aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGCAggggggggggggg                                                                                                              >  1:106090/1‑148 (MQ=255)                                       ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCCGGAAGGGGAGggggggggggcg                                                                                                              >  2:412106/1‑149 (MQ=255)                                         ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAggggggggggggggtt                                                                                                           >  1:326353/1‑145 (MQ=255)                                            gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGAAAAATGTGCTGAAAAGGCGGGTGGCGGTGGGGtt                                                                                                            >  2:348453/1‑146 (MQ=255)                                             ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCTGAAAGGGCAGGGGGGGGGGGGGTTTGTCAt                                                                                                      >  1:251203/1‑151 (MQ=255)                                                gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGGGCTGAAAGGGGAGGGGGCGGGGGGGGTTggcctttc                                                                                                   >  1:371900/1‑144 (MQ=255)                                                gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCAGCCGGGCGGTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                   <  2:100434/151‑1 (MQ=255)                                                      aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGACAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                <  2:26997/148‑1 (MQ=255)                                                         aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGTGGCGGGGGGGGTTGTCATTCCGaaa                                                                                               >  2:519613/1‑146 (MQ=255)                                                           gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCCTTCCGGAagag                                                                                            >  2:238267/1‑147 (MQ=255)                                                           gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGgaa                                                                                               >  2:243717/1‑144 (MQ=255)                                                                 aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGt                                                                                   <  1:581429/150‑1 (MQ=255)                                                                   cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                                <  2:36667/151‑1 (MQ=255)                                                                       gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                             <  2:597754/150‑1 (MQ=255)                                                                        ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGTGGTGAAAAGGGAGGGGGGGGGGGCGGTTGTTATTTCGAAAGAGAGCGACAGGCTgg                                                                               >  2:16708/1‑147 (MQ=255)                                                                           ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGTTTGTTATTTCCGAAGAGAGGGACAGTCTgg                                                                               >  1:521195/1‑144 (MQ=255)                                                                            gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTTTCATTCCGAAAGAGAGCGACAGGCTgg                                                                               >  2:475137/1‑143 (MQ=255)                                                                                 ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGTTTGTCATTCCGAAAAAGAGCGACAGGCTGGGAGATCggg                                                                      >  2:493210/1‑145 (MQ=255)                                                                                        gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc                                                           <  2:619095/151‑1 (MQ=255)                                                                                         aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGATGCGCTTGAAGGGCAGGGGGCGGGGGGGGTTGTTATTTCGAAAGAGAGCGACAGTCTGGGAGGTCGTGATGGGGCGCACt                                                          >  2:335037/1‑151 (MQ=255)                                                                                          cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGGGCGGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGGAAGGGAGCGACAGGCTGGAAGATCGGGGTGTGGGGCGCTc                                                         >  1:631496/1‑151 (MQ=255)                                                                                           gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGAAAGAAGCGCTGTAAGGGCCGGTGGCGGGGGCGGTTGTGCTTTCGCAAGAAAGCGCCAGTGTGGGAGGTTGCGATGTggggtgc                                                           >  2:223967/1‑143 (MQ=255)                                                                                            cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                          <  1:214132/148‑1 (MQ=255)                                                                                            cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                       <  1:106261/151‑1 (MQ=255)                                                                                              cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                                     <  2:763561/151‑1 (MQ=255)                                                                                                    tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                <  2:718569/150‑1 (MQ=255)                                                                                                      aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                >  1:210163/1‑148 (MQ=255)                                                                                                         agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGTGGAAAATGCGCTGAAAGGGCCGGGGGCGGGGGCGTTTGTCGTTCCGAAAGAGAGCGGCAGTCTGGAAGAACGGGATGTGGCGCCCTCGCAAGAGAAGGCg                                            >  2:542607/1‑149 (MQ=255)                                                                                                         agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                            >  1:554877/1‑149 (MQ=255)                                                                                                                  ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCt                                 <  1:481971/151‑1 (MQ=255)                                                                                                                    aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCCGGAAGGGGCGGGGGGGGTGGGGGTTTGCATTCCGGAAGAGAGCGGCAGTCTGGGAGAACGGGAGGTGGCGCGCTCGCAAGAGGAGGGGGATATGGGGCtgg                               >  2:187744/1‑151 (MQ=255)                                                                                                                        aTCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGAAAGATGCGCTGAAAGGGGCGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGGAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGGTTATGGGGCtgggggg                           >  2:584139/1‑150 (MQ=255)                                                                                                                          ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                         <  1:519978/151‑1 (MQ=255)                                                                                                                             ggCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTGGCATTCCGCAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCACTCGCCAGAGAAGGGGATTATGGGGCcggggggc                          >  2:133575/1‑145 (MQ=255)                                                                                                                                gTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGa                   <  1:78089/151‑1 (MQ=255)                                                                                                                                                 ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc  <  1:510686/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354136‑354430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑