Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,378 G→T R218S (CGT→AGT)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,3780GT96.4% 212.5 / 0.1 55R218S (CGT→AGT) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (23/30);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

AGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGAC  >  NC_000913/3440232‑3440521
                                                                                                                                                  |                                                                                                                                               
agagCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATc                                                                                                                                             <  1:674343/151‑1 (MQ=255)
 gagCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCg                                                                                                                                            <  1:489582/151‑1 (MQ=255)
  agCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGc                                                                                                                                           <  1:42860/151‑1 (MQ=255)
   gCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGc                                                                                                                                           <  2:727647/150‑1 (MQ=255)
      gACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCctct                                                                                                                                       <  2:656911/151‑1 (MQ=255)
        cAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTc                                                                                                                                     <  2:9843/151‑1 (MQ=255)
           tCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAgg                                                                                                                                  >  1:215393/1‑151 (MQ=255)
              aTTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATc                                                                                                                               <  1:15976/151‑1 (MQ=255)
                 ccAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGa                                                                                                                             <  2:679817/150‑1 (MQ=255)
                    gATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATtgt                                                                                                                         <  1:658422/151‑1 (MQ=255)
                     aTCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATtgtg                                                                                                                        <  2:517091/151‑1 (MQ=255)
                           aaCAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTg                                                                                                                  >  2:56853/1‑151 (MQ=255)
                            aCAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTgg                                                                                                                 >  2:232166/1‑151 (MQ=255)
                            aCAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTgg                                                                                                                 <  2:88223/151‑1 (MQ=255)
                                     agcagGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCAttt                                                                                                        >  1:360675/1‑151 (MQ=255)
                                     agcagGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCAttt                                                                                                        <  1:455145/151‑1 (MQ=255)
                                           atcggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGa                                                                                                  <  2:562038/151‑1 (MQ=255)
                                            tcggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGAc                                                                                                 >  2:649447/1‑151 (MQ=255)
                                             cggatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGAc                                                                                                 <  2:364827/150‑1 (MQ=255)
                                               gatcgAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAg                                                                                              <  2:295656/151‑1 (MQ=255)
                                                      ctctGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTcc                                                                                       >  2:260351/1‑151 (MQ=255)
                                                         tGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAgg                                                                                    >  1:4158/1‑151 (MQ=255)
                                                          ggTTTCTCTTCTTTCACTTCAGGCTGACGTACATCTCGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGGCGCACGACGAATCGCCTCTGCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAgg                                                                                    <  2:23019/150‑1 (MQ=255)
                                                           gTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTc                                                                                  >  1:246572/1‑151 (MQ=255)
                                                           gTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTc                                                                                  >  2:531896/1‑151 (MQ=255)
                                                             ttCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCgg                                                                                >  2:319479/1‑151 (MQ=255)
                                                                 cttcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGt                                                                               <  1:283522/148‑1 (MQ=255)
                                                                        cACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCt                                                                     <  1:720042/151‑1 (MQ=255)
                                                                        cACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCt                                                                     >  1:236785/1‑151 (MQ=255)
                                                                         aCTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCt                                                                     >  2:473172/1‑150 (MQ=255)
                                                                             cAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACAcg                                                                <  1:39228/151‑1 (MQ=255)
                                                                              aGGCTGACGTACATCACGTAAGTGAACGAAAGCTTCCAGGTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTAGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACAcg                                                                >  2:331215/1‑150 (MQ=255)
                                                                              aGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACAcgc                                                               <  1:173197/151‑1 (MQ=255)
                                                                                  tGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCgctg                                                           >  2:361019/1‑151 (MQ=255)
                                                                                   gACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCgct                                                            <  1:589372/149‑1 (MQ=255)
                                                                                     cGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCtt                                                        <  1:678678/151‑1 (MQ=255)
                                                                                         cATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCaa                                                     >  2:307246/1‑150 (MQ=255)
                                                                                               gTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGt                                               >  2:69257/1‑150 (MQ=255)
                                                                                                tAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGt                                               >  1:317026/1‑149 (MQ=255)
                                                                                                  aGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGc                                           <  1:276545/151‑1 (MQ=255)
                                                                                                       aCGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATAc                                      <  1:684031/151‑1 (MQ=255)
                                                                                                         gAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGc                                    <  2:686577/151‑1 (MQ=255)
                                                                                                           aaGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTc                                  >  1:751156/1‑151 (MQ=255)
                                                                                                           aaGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTc                                  >  1:528293/1‑151 (MQ=255)
                                                                                                           aaGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTc                                  <  1:291895/151‑1 (MQ=255)
                                                                                                             gCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCca                                <  1:558423/151‑1 (MQ=255)
                                                                                                               ttCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCcac                               >  1:577195/1‑150 (MQ=255)
                                                                                                                   aGTTGTTCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGc                          <  2:203899/151‑1 (MQ=255)
                                                                                                                       gttCAGCCAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGt                       <  1:333366/150‑1 (MQ=255)
                                                                                                                             ccAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGc                >  2:52245/1‑151 (MQ=255)
                                                                                                                             ccAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGc                <  2:758362/151‑1 (MQ=255)
                                                                                                                             ccAGAATGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGc                <  2:291935/151‑1 (MQ=255)
                                                                                                                                   tGGTTGCCGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGAc          >  2:69829/1‑151 (MQ=255)
                                                                                                                                          cGCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcagc      >  2:291389/1‑148 (MQ=255)
                                                                                                                                           gCACGACTAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGAc  <  2:526863/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGAC  >  NC_000913/3440232‑3440521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑