Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,536,332 +T coding (253/720 nt) ompR ← response regulator in two‑component regulatory system with EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,536,3291.T98.7% 312.7 / NA 79G86R (GGG→AGG) ompRresponse regulator in two‑component regulatory system with EnvZ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base T (41/37);  minor base G (0/1);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGC  >  NC_000913/3536179‑3536476
                                                                                                                                                       |                                                                                                                                                   
ccTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTcccc                                                                                                                                                      >  2:1203842/1‑151 (MQ=255)
   ctTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCttt                                                                                                                                                  >  1:1243396/1‑151 (MQ=255)
     tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCtttt                                                                                                                                                 <  1:695634/150‑2 (MQ=255)
     tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCtttt                                                                                                                                                 <  1:461705/150‑2 (MQ=255)
     tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCtttt                                                                                                                                                 <  2:1215728/150‑2 (MQ=255)
     tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCtt                                                                                                                                                   <  1:1080518/148‑1 (MQ=255)
      ccTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCttttcg                                                                                                                                               <  1:728413/151‑4 (MQ=255)
       cTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCttttcgc                                                                                                                                              >  2:485170/1‑147 (MQ=255)
         gtgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCg                                                                                                                                             >  2:722763/1‑151 (MQ=255)
           gACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGt                                                                                                                                            <  2:863899/150‑1 (MQ=255)
              ggTCCGCCTGGCAGTTCGTTCGCCGGACGACGCAGCACCGCACGGATACGGGCCAGCAGCTCACGCAGGTTAAACGGTTTTGGCATGCAGACGTCGGCGCCAATCTCCAGGCCTACGACACGGTCCACTTCTTCCCCTTTTCGCCGTCAcc                                                                                                                                        >  2:1107421/1‑151 (MQ=255)
                tGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCAt                                                                                                                                      >  1:1212689/1‑151 (MQ=255)
                    ccTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAAt                                                                                                                                   <  2:965622/150‑1 (MQ=255)
                     cTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATg                                                                                                                                  >  2:291948/1‑150 (MQ=255)
                     cTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATg                                                                                                                                  >  2:309410/1‑150 (MQ=255)
                      tGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGAt                                                                                                                                >  1:209998/1‑151 (MQ=255)
                        gCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATc                                                                                                                               >  2:1133748/1‑150 (MQ=255)
                        gCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCg                                                                                                                              <  1:981954/151‑1 (MQ=255)
                        gCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCg                                                                                                                              <  1:546809/151‑1 (MQ=255)
                         cAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCgg                                                                                                                             >  1:120286/1‑151 (MQ=255)
                              cgttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATc                                                                                                                         <  2:744538/150‑1 (MQ=255)
                              cgttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATc                                                                                                                         <  1:1079283/150‑1 (MQ=255)
                               gttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCgg                                                                                                                       >  1:45707/1‑151 (MQ=255)
                                ttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCggg                                                                                                                      >  1:862078/1‑151 (MQ=255)
                                   gCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTg                                                                                                                   >  1:163951/1‑151 (MQ=255)
                                   gCCTAACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGt                                                                                                                     <  1:1184898/149‑1 (MQ=255)
                                       gacgacGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGctct                                                                                                               <  1:1162153/151‑1 (MQ=255)
                                         cgacgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGa                                                                                                             <  2:456064/151‑1 (MQ=255)
                                            cgCACCTCCGCACGGTTACGGACCAGCAGTGCTCGCGGGTTAATCGGTCTTGGAATGTAGTCGTCAGCGCCAATCTCAAGGCCTACGATACGGTCCGCTTCTTCCCCGTTTGGCCGTCGCCATAATGCTCGGCGTCGGGTTGCTCTGACTa                                                                                                          <  2:958723/151‑1 (MQ=255)
                                               agcaCCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACg                                                                                                        >  2:439028/1‑150 (MQ=255)
                                                gcaCCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGaa                                                                                                      <  1:1230675/151‑1 (MQ=255)
                                                       aCGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCg                                                                                               >  1:817974/1‑151 (MQ=255)
                                                       aCGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCg                                                                                               <  1:278606/151‑1 (MQ=255)
                                                       aCGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCg                                                                                               <  1:1160788/151‑1 (MQ=255)
                                                          gATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCa                                                                                            <  1:984290/151‑1 (MQ=255)
                                                           aTACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCaa                                                                                           <  1:439367/151‑1 (MQ=255)
                                                             aCGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAAt                                                                                         >  2:646613/1‑151 (MQ=255)
                                                              cGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATc                                                                                        <  1:452937/151‑1 (MQ=255)
                                                                          tCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCt                                                                            >  2:632339/1‑151 (MQ=255)
                                                                             cgcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCa                                                                         <  2:100101/151‑1 (MQ=255)
                                                                              gcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCAc                                                                        >  1:272068/1‑151 (MQ=255)
                                                                              gcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCAc                                                                        >  2:1160409/1‑151 (MQ=255)
                                                                              gcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCAc                                                                        >  1:612665/1‑151 (MQ=255)
                                                                                 ggTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCAcc                                                                       >  1:1032106/1‑149 (MQ=255)
                                                                                  gTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAgg                                                                    <  1:465626/151‑1 (MQ=255)
                                                                                    tAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGt                                                                   <  1:1079244/150‑1 (MQ=255)
                                                                                      aaCGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAAc                                                                >  1:1205693/1‑151 (MQ=255)
                                                                                          gTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACAtt                                                             >  1:323589/1‑150 (MQ=255)
                                                                                          gTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTa                                                            <  1:229985/151‑1 (MQ=255)
                                                                                          gTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTa                                                            <  2:135709/151‑1 (MQ=255)
                                                                                             ttGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTaaa                                                          <  1:1133769/150‑1 (MQ=255)
                                                                                                gAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACTGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATcc                                                       <  1:214670/150‑1 (MQ=255)
                                                                                                gAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCa                                                      <  2:616130/151‑1 (MQ=255)
                                                                                                  aTGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGt                                                    >  1:495181/1‑151 (MQ=255)
                                                                                                   tGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGt                                                    >  2:595060/1‑150 (MQ=255)
                                                                                                   tGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGt                                                    >  1:165945/1‑150 (MQ=255)
                                                                                                    gTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTAc                                                  >  2:62923/1‑151 (MQ=255)
                                                                                                      aGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTAcc                                                 >  2:43397/1‑150 (MQ=255)
                                                                                                        tcgtcAGCGCCAATCTCTAGGCCTACGATGCGGTCCTCTTCTTCCCCTTTTCGCCGTCGCCATGATGTGCGGCATCGGGTTGCGCTGACTACGAAGACGTCGGCAAATCGAGAAGCCATCTTGGCCAGGTAGCATTAAATCCAGTAcc                                                 <  2:952668/148‑1 (MQ=255)
                                                                                                        tcgtcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCAt                                               >  1:219186/1‑150 (MQ=255)
                                                                                                         cgtcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATaa                                             <  1:418746/151‑1 (MQ=255)
                                                                                                         cgtcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATaa                                             <  1:543970/151‑1 (MQ=255)
                                                                                                         cgtcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATa                                              <  1:882580/150‑1 (MQ=255)
                                                                                                          gtcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATa                                              >  1:1140895/1‑149 (MQ=255)
                                                                                                           tcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATa                                              >  1:138689/1‑148 (MQ=255)
                                                                                                            cAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGa                                           <  2:165943/150‑1 (MQ=255)
                                                                                                               cgcCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATgg                                        >  1:82421/1‑150 (MQ=255)
                                                                                                               cgcCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATgg                                        >  1:816770/1‑150 (MQ=255)
                                                                                                               cgcCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGa                                       <  2:1044807/151‑1 (MQ=255)
                                                                                                                gcCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGa                                       >  2:58258/1‑150 (MQ=255)
                                                                                                                gcCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGa                                       >  1:690838/1‑150 (MQ=255)
                                                                                                                 ccAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGaaa                                     <  2:966758/151‑1 (MQ=255)
                                                                                                                  cAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAg                                    <  1:101423/151‑1 (MQ=255)
                                                                                                                     tctcCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGAtt                                 >  1:875532/1‑151 (MQ=255)
                                                                                                                        ccAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGAt                                  <  1:660379/147‑1 (MQ=255)
                                                                                                                         cAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCa                               <  1:1072714/149‑1 (MQ=255)
                                                                                                                            gCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGt                          <  1:1222168/151‑1 (MQ=255)
                                                                                                                             ccTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTc                         >  1:173542/1‑151 (MQ=255)
                                                                                                                               tACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTc                         >  2:133105/1‑149 (MQ=255)
                                                                                                                                aCGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTca                        <  2:590317/149‑1 (MQ=255)
                                                                                                                                aCGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTca                        <  2:862064/149‑1 (MQ=255)
                                                                                                                                  gATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTcagc                      >  1:560461/1‑149 (MQ=255)
                                                                                                                                   aTACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAgg                   >  2:403753/1‑151 (MQ=255)
                                                                                                                                      cGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGc                  <  1:184143/149‑1 (MQ=255)
                                                                                                                                         tCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATcc             >  1:348467/1‑151 (MQ=255)
                                                                                                                                               tcttcCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGt       >  1:1121795/1‑151 (MQ=255)
                                                                                                                                               tcttcCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGt       >  1:998723/1‑151 (MQ=255)
                                                                                                                                                 tcccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTc     <  2:791921/148‑1 (MQ=255)
                                                                                                                                                 tcccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCt    >  1:748478/3‑151 (MQ=255)
                                                                                                                                                   ccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGc  >  2:211689/1‑151 (MQ=255)
                                                                                                                                                   ccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGc  <  2:1087003/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGC  >  NC_000913/3536179‑3536476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: