| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,478,324 | 0 | T | G | 45.8% | 72.3 / 13.9 | 48 | G33G (GGT→GGG) | ynbB | putative CDP‑diglyceride synthase |
| Reads supporting (aligned to +/- strand): ref base T (4/22); new base G (22/0); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.67e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGT > NC_000913/1478179‑1478467 | gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAgg < 2:743579/150‑1 (MQ=255)gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGt < 1:99728/151‑1 (MQ=255) cGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAAGGGGGGAgggt > 1:136565/1‑149 (MQ=255) tCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGGGAG‑AAAAAGGGGGGGGGGTACATTACGTAtt > 1:260527/1‑150 (MQ=255) ggAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTT‑CCGGGGGG‑AAAAAGGGGGGAGGGTACATTACGTATTCGt > 1:657008/1‑151 (MQ=255) ggAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCCGGGGGG‑AAAGGGGGGGGGGGTTA‑ATTACGTAtttg > 2:639668/1‑148 (MQ=255) tgtgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCCGAGAG‑AAAAGGGGGGGGGGGTACATTTCATATTCGTACCt > 1:878269/1‑151 (MQ=255) gtgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑ATT‑CCTGGGGG‑GAAAGGGGGGGAGGTTAATTTTCGTAttttttacct > 2:854523/1‑143 (MQ=255) tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTT‑CCGGAGAG‑AAAAGGGGGGGGGGGTTCATTACGTATTTGTACCt > 2:559191/1‑149 (MQ=255) ttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑TCGGGGGG‑GAAAGGGGGGGGGGGTACATTTAGTATTTGTACCtggggg > 1:25158/1‑151 (MQ=255) gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTT‑ACGGGGAG‑AAAAGGGGGGGAGGGTTTCTTTCGACTTTGTATCTGGTGGGGCAt > 2:197009/1‑149 (MQ=255) gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATc < 2:1053297/150‑1 (MQ=255) gcgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTc < 2:1040075/151‑1 (MQ=255) cgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑GTA‑CCGGGGAG‑AAAAAGGGGGGGGGTGGAATTATGGATTTGTATCTTGTgggg > 2:792595/1‑143 (MQ=255) gAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑TCAGGGTG‑AAAAGGGGGGGAGGTTTTAATACCTCTTCGGCCCTGGTGGGGGATCGTCGtt > 2:106968/1‑149 (MQ=255) ataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTT‑CCGGGGAG‑GAAAGGGGGGGAGGGTACATTACGTATTCGTACCTGGTGGGGGGTCGTCATTTGttttt > 1:366074/1‑151 (MQ=255) ataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGtttt < 1:119415/150‑1 (MQ=255) cTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑ACGGAGAG‑AAAAGGGGGGGGGGGTACATTACGTATTCGTACCTGGTGGGGGGTCGTCCTTTGTTTTTTAAt > 1:133193/1‑149 (MQ=255) aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTA‑CCGG‑GAG‑AAAAGGGGGGGGGGGTACCTTTCGTTTTTGTTCCTGGTGGGGGATCGTCATTTTTTTTTTTATGGTGGttt > 2:735758/1‑150 (MQ=255) aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGGGAG‑AAAAGGGGGGGGGGGTAAATTACGTATTTGTACCTGGGGGGGGGTCGTCATTTGTTTTTTAATGGTGAttt > 1:306100/1‑150 (MQ=255) gCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCAc < 2:1223266/151‑1 (MQ=255) gCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGGGGGGGTACATTACGTATTCGTACCTGGGGGGGCATCGTCATTTTTTTTTTTATGGGGATTTTAGGGCCAc > 1:1098744/1‑151 (MQ=255) acTTTTCGCGTTGTTAATTTTAGCAACGCTGCTAAACCGTTTTCTGCTATGGCGG‑TTT‑CCGGGGAG‑GAAAAGGGGGGAGGTTTTTTTCAGCTTTCTTCTATTGTGGGGGATCATCCTTTGTTTTTCACTTGTGGTTTTTAGTCCACGcag > 2:999516/1‑148 (MQ=255) tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAt < 2:54542/150‑1 (MQ=255) gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTT‑CCGGGGAG‑GAAAGGGGGGGGGGGTACATTAAGTATTTGTACCTGGGGGGGCGACGTCATTTGTTTTTTTATGGGGATTTCAGGTCCACGCTGGGtgaagtt > 1:332212/1‑150 (MQ=255) aTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGtttt < 1:730163/151‑1 (MQ=255) tttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGttttt < 1:718909/150‑1 (MQ=255) gCATCGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATGACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTCTTTgcg < 2:447218/150‑1 (MQ=255) cAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCCGGGAGAAAAAGGGGGGGGGGTTAC‑TTAAGTATTTGTAACTGGTGGGGCCTCGTCATTTGTTTTTTAATGGGGATTTTACGTCCACGCTGGATGACGTTGACGtttttt > 1:848600/1‑146 (MQ=255) aaCGCTGATAAACCGTTTTCTGCTTTGGCGG‑TTA‑CCGGAGAG‑AAAAAGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcg < 2:883376/148‑1 (MQ=255) aaCGCTGATAAACCGTTTTCTGCTATGGCGG‑TTT‑CCGGGGAG‑AAAAGGGGGGGAGGGTACATTTCGTATTCGGACCTGGTGGGGGAACGGCCTTTGTTTTTTTATGGGGGGTTTAGGTCCACGCTGGGTGACGTTTTTGtttttt > 2:782066/1‑145 (MQ=255) aCGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGGGAG‑AAAAGGGGGGGAGGGTACATTACTTATTCGTACCTGGGGGGGGATCGTCATTTTTTTTTCTATGGTGATTTTAGGGCCACGCTGGATGACGTTGACGtttttt > 1:321059/1‑144 (MQ=255) gCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGGGAG‑AAAAGGGGGGGAGGGTATACTACGTATTTTTACCTTGTGGGGGATCGTCATTTGTTTTTTATTGGGGTTTTTAGGACCACGCCGGGTGACGTTGTCGttttttttggcgtt > 2:209665/1‑142 (MQ=255) gATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAg < 2:551356/150‑1 (MQ=255) gCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa < 2:73190/149‑1 (MQ=255) gCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa < 1:1021365/149‑1 (MQ=255) aTGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTCCATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTGCAATGGTGATTTCAGGTCCAAGATGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa < 2:937517/146‑1 (MQ=255) aTGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAg < 2:1056025/149‑1 (MQ=255) ggCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAat > 2:332218/1‑150 (MQ=255) g‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt < 1:177197/151‑1 (MQ=255) a‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc < 2:996378/150‑1 (MQ=255) a‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc < 1:403033/150‑1 (MQ=255) a‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg > 2:146845/1‑151 (MQ=255) ggAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCtt < 1:817093/151‑1 (MQ=255) agag‑aAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat > 2:882870/1‑151 (MQ=255) ag‑aAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTatat > 2:582623/1‑151 (MQ=255) aaaaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCt < 2:52729/151‑1 (MQ=255) aaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt < 2:25158/151‑1 (MQ=255) | GCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTA‑CCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGT > NC_000913/1478179‑1478467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |