Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,112,5990TG43.4% 142.1 / 21.1 85G95G (GGT→GGG) yiiRDUF805 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (11/36);  new base G (36/0);  total (49/36)
Fisher's exact test for biased strand distribution p-value = 6.84e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAG  >  NC_000913/4112450‑4112748
                                                                                                                                                            |                                                                                                                                                            
aCCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGgtg                                                                                                                                                             <  2:1231603/151‑1 (MQ=255)
  cgcgGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTGCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGgt                                                                                                                                                              <  2:347805/148‑1 (MQ=255)
   gcggcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑t                                                                                                                                                         <  2:290893/151‑1 (MQ=255)
          tttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑t                                                                                                                                                <  1:1103194/150‑1 (MQ=255)
            tGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑gg                                                                                                                                             <  2:230733/150‑1 (MQ=255)
                 tgtgtgCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGG‑GGTTTTACCGGGGGTC‑T‑GG‑CAA‑T‑GGGCgggg                                                                                                                                       >  1:643834/1‑149 (MQ=255)
                    gtgCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑TGAT‑C‑T‑GGG‑‑GGTTTTTTCGGGGGTT‑T‑GG‑CAA‑T‑Tgggggggggg                                                                                                                                    >  1:680473/1‑151 (MQ=255)
                     tgcttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑TGGG‑GATTTTTCCCGGGGTC‑T‑GG‑CAA‑T‑Ggggggggggg                                                                                                                                    >  1:631296/1‑150 (MQ=255)
                     tgcttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGG‑GGTTTTTCCGGGGGGT‑T‑GG‑CAA‑T‑Ggggggggggg                                                                                                                                    >  1:324115/1‑150 (MQ=255)
                       cttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑C‑GGGG‑GGTTTTTCCCGGGGTC‑T‑GG‑GAA‑A‑TGGGGGGGGGGCgg                                                                                                                                 >  2:820641/1‑150 (MQ=255)
                        ttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGG‑A‑AA‑A‑G‑GGGG‑GGTTTTACCGGG‑GTC‑T‑TG‑CAA‑A‑GGGCGGGGGGGCg                                                                                                                                  >  2:684798/1‑149 (MQ=255)
                         tgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGtt                                                                                                                                <  2:820872/150‑1 (MQ=255)
                         tgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGt                                                                                                                                 <  2:213769/149‑1 (MQ=255)
                          gctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGC‑GGTTTTTCCCGGGGTC‑T‑GG‑CAA‑T‑GGGGGGGGGGGGGgttt                                                                                                                              >  1:956104/1‑151 (MQ=255)
                          gctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGG‑GATTTTTCCCGGGGTC‑T‑GG‑CAA‑T‑GGGCGGGGGGGGGgttt                                                                                                                              >  1:594411/1‑151 (MQ=255)
                             ctGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTg                                                                                                                             <  2:246930/149‑1 (MQ=255)
                                    aCAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCAGTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GGTTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGTTAAGGCGTTTTGTCCCGAC‑g                                                                                                                    <  2:701877/150‑1 (MQ=255)
                                     cAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑Gtt                                                                                                                  <  2:682124/151‑1 (MQ=255)
                                       gCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑C‑GGGG‑GGTTTTTCCGGGGGGT‑T‑GG‑CAA‑T‑GGGGGGGGGGGGGTTTTGTTCCggc                                                                                                                      >  2:848095/1‑144 (MQ=255)
                                          gCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTc                                                                                                             <  1:1053165/151‑1 (MQ=255)
                                            aGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGG‑GATTTTTCCGGGTGTC‑T‑GG‑CGA‑T‑GGGGGGTGGGGGGTTTTGTTCCGGC‑GGTGtttat                                                                                                            >  1:448339/1‑148 (MQ=255)
                                            aGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑C‑GGGG‑GGTTTTTCCCGGGGGC‑T‑GG‑GAA‑A‑TGGCGGGGGGGGGTTTTTTccccgcctttgttt                                                                                                              >  2:3951/1‑138 (MQ=255)
                                              tAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑A‑GGGG‑GGATTTACCGGGGGGG‑TGGG‑AAA‑T‑GGGGGGGGGGGGGTTTGGT‑CCGAC‑GTTGGTTCTgg                                                                                                          >  2:304979/1‑150 (MQ=255)
                                               aaCTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGt                                                                                                         <  1:341949/150‑1 (MQ=255)
                                                aCTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGGTGCTGCCGGC‑GGG‑G‑AAGC‑T‑GGGG‑CTTTTAACCGGGGGT‑‑T‑GG‑CCA‑T‑GGGGGGTGGGGGGGTTTTTTCCCAC‑ggctctttctgggg                                                                                                       >  2:182278/1‑138 (MQ=255)
                                                  tGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGC‑GATTTTTCCGGGGGTC‑T‑GG‑GAA‑T‑GGGCGGGGGGGGGGTTTTTTCCGAC‑CGTGAtttttgggat                                                                                                      >  1:1048268/1‑142 (MQ=255)
                                                  tGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGG‑GGTTTTTCCCGGGGTCTT‑GG‑CAA‑‑‑GGGGGGTGGGGGGGTTTTTCCCCAC‑GTTGGTTTTtgtggt                                                                                                      >  1:73932/1‑148 (MQ=255)
                                                     tAAGCGCTTGCATGATCGCTGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAG‑T‑GGGCGGTGGGGCGGTTTGTCCCGAC‑GTTGATTCTGGTgatgat                                                                                                   <  2:825807/150‑1 (MQ=255)
                                                     tAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCGTGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTgatgat                                                                                                   <  2:974386/150‑1 (MQ=255)
                                                      aaGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGC‑GGTTTTTACGGGGGGT‑T‑GG‑CAA‑T‑GGGGGGGGGGGGGGTTTTGCCCGAC‑GTTTGTTCTGGGGGTGAAGc                                                                                                 >  2:535843/1‑151 (MQ=255)
                                                      aaGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATCGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑C‑GGGG‑GAATTTTCCGGGGGGC‑C‑GG‑CGA‑A‑GGGCGGGGGGGGGGTTTGTTCCGGC‑GGTGGTTCTgggg                                                                                                        >  1:1156953/1‑142 (MQ=255)
                                                           cTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGG‑GGTTTTTCCGGGGGTC‑T‑GG‑CAA‑T‑GGGGGGGGGGGGGGTTTTTCCCCAC‑GTTGTTTCTGGGGATGATGCTTAt                                                                                             >  1:188725/1‑150 (MQ=255)
                                                           cTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑TGGG‑GGTTTTAACGGGGGTC‑T‑GG‑GAA‑T‑GGGCGGGGGGGGGGTTTGTCCCGAC‑GGTGATTTTtgtggttgtggtt                                                                                               >  1:1037330/1‑139 (MQ=255)
                                                             tGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATc                                                                                            <  2:689676/149‑1 (MQ=255)
                                                             tGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGG‑GAGTTTAACCGGGGGC‑T‑GG‑GCA‑A‑GGGCGGGGGGGGGTTTTTTCCCGAC‑TTTGGTTCTTGGGGTGATGCTTTTCgg                                                                                          >  2:426462/1‑150 (MQ=255)
                                                                   aTCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTgg                                                                                    <  1:973680/151‑1 (MQ=255)
                                                                    tCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGG‑GGATTTTTCGGGGGTT‑T‑GG‑GAATT‑GGGGGGGGGGGGGTTTTTTCCC‑CC‑GTTGATTTTTGGGGTGATTGTTATCGaaatt                                                                                      >  1:25629/1‑144 (MQ=255)
                                                                       cgGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCg                                                                                <  1:854127/151‑1 (MQ=255)
                                                                        gggCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGG‑GGTTTTTCCGGGGGTC‑TGGG‑CAT‑G‑GGGGGGGGGGGGGTTTTTTCCCG‑C‑GGTTATTTTGGGGATGGTGCTTATCGATCtttg                                                                                    >  1:253726/1‑144 (MQ=255)
                                                                           cGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AACC‑T‑GGGG‑GTTTTTACCGGGGGCT‑G‑GG‑AAA‑T‑GGGGGGGGGGGGGTTTTTTCCCGCG‑GTTGTTTTTGG‑GGTGATGGTTATTGGTATTGGGGCGttt                                                                             >  1:164943/1‑150 (MQ=255)
                                                                           cGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTttg                                                                            <  1:1143700/151‑1 (MQ=255)
                                                                            gTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGC‑GATTTTTCCCGGGGTC‑T‑GG‑CAA‑T‑GGGCGGGGGGGGGGTTTGTCCCGAC‑GTTTATTTTGGGGGTGATGCTTTTCGGTTTTTGTGGGGttgt                                                                           >  1:888021/1‑151 (MQ=255)
                                                                            gTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑C‑GGGC‑GGTTTTATCCGGGGTT‑C‑GG‑CAA‑A‑GGGCGGGGGGGGGGTTTTTTCCCTC‑CTTGGTTTTGGGGATGATGGTTTTTCATCTTTGGGGGttt                                                                             >  1:525316/1‑149 (MQ=255)
                                                                              tCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTttgtt                                                                          <  2:119684/150‑1 (MQ=255)
                                                                               ccGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTgg                                                                        <  2:119053/151‑1 (MQ=255)
                                                                               ccGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGG‑GGTTTTTACGGGGGTC‑T‑GG‑CCA‑T‑GGGGGGGGGGGGGGTTTTTTCCGAC‑GGTGGTTCTGGGGATGATGCTTTTCGATCTTTGTGCGGttttttg                                                                        >  1:687476/1‑149 (MQ=255)
                                                                                cGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGG‑GGTTTTACCCGGGGGC‑T‑GG‑CAA‑T‑GGGGGGGGGGGCGGTTTTTCCCGAC‑GGTGATTTTGGGGGTGATGCTTATCGATCTTTGTGGGTttgtttg                                                                        >  1:1125424/1‑148 (MQ=255)
                                                                                cGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑T‑GGGC‑GGTTTTTCCCGGTGGT‑T‑GG‑CAA‑T‑GGGGGGGGGGGGGGTTTTTTCCCCC‑GGTGTTTTTGGTGGTGGTGGTTTTTGATCTTGGGGCGgttgtt                                                                          >  2:6157/1‑148 (MQ=255)
                                                                                 ggCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGca                                                                      <  2:376276/151‑1 (MQ=255)
                                                                                   cgcATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑C‑GGGG‑GGTTTTTCCGGGGGGC‑TGGG‑AAA‑G‑GGGGGGGGGGGGGTTTTTTCCCG‑C‑GGTTATTTTGGTGGTGGTGGTTTTCGGTCTTTGGGCGGTTGTTGGca                                                                      >  2:279822/1‑149 (MQ=255)
                                                                                      aTGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑aa                                                                <  1:823777/151‑1 (MQ=255)
                                                                                      aTGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑aa                                                                <  1:585588/151‑1 (MQ=255)
                                                                                      aTGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑GA‑C‑T‑GGGG‑GGATTTTCCGGGGGGC‑T‑GG‑CAA‑T‑GGGGGGGGGGGCGGTTTGTTCCCACGGTTGTTTTTGG‑GGTGATGGTTATTGTTTTTGGTGCGTTTTTTTGgacac                                                                   >  1:1068144/1‑149 (MQ=255)
                                                                                        gggCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAg                                                               <  2:1125404/150‑1 (MQ=255)
                                                                                         ggCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGc                                                             <  1:1219990/151‑1 (MQ=255)
                                                                                          gCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTT‑AA‑C‑T‑TGG‑‑GAGTTTTCCGGGGGTC‑T‑GGCCAA‑G‑GGGGGGGGGGGGGTTTTTCCCCAAC‑GTT‑TTTTTTGTGGTGGTGGTTTTCGATCTTTGGGGGGTTTTTGGCATAC‑CAgggg                                                            >  1:804579/1‑149 (MQ=255)
                                                                                            aTTTCTGATGATTGTGGCGTGGATGCTGCTGGG‑GGG‑T‑AA‑CAT‑GTGG‑AGTTTTCCCGGGGGCC‑T‑‑G‑CAA‑C‑TGGGGGGGGGGGGGTTTGTTCCGAG‑CTTGGTTTTGGTGGAGGGGCTCGTCGGTCTTTGTGGGGTTGTTGGGAATC‑AAgg                                                              >  1:353043/1‑147 (MQ=255)
                                                                                              ttCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGC‑GCTTTTTCCCGGGGGC‑T‑GG‑GCA‑T‑TGGCGGGGGGGGGGTTTTTCCCCGC‑GTTTATTTTGGGGGTGGTGGTTTTTGGTCTTTGGGGGGTTGTTGGgaccccaagcggaa                                                          >  2:933373/1‑139 (MQ=255)
                                                                                                  gatgatTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AACC‑G‑GGGG‑GATTTTACCGGG‑GTC‑T‑GG‑GGA‑T‑GGGGGGGGGGGGGGTTTTTGCCGAC‑GGTGGTTCTTGGGGTGGTGGTTATCGGTCTTTGTGGGGTTGTTGGGACACAAAGGGGaaaaaa                                                      >  2:358962/1‑148 (MQ=255)
                                                                                                   atgatTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑C‑GGGG‑GATTTTTCCCGGGGTC‑T‑GG‑CAA‑T‑GGGGGGGGGGGGGGTTTTTCCCGGC‑GGTGATTCTGGGGGTGATGCTTTTCGATTTTTGTGCGTTTTTTTGGACAC‑AAGGCGGaaataaa                                                    >  1:1185195/1‑150 (MQ=255)
                                                                                                    tgatTGTGGCGTGGATGCTGCTGTC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAaaataaat                                                   <  1:1219480/150‑1 (MQ=255)
                                                                                                    tgatTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑A‑T‑TGGCGGTTTTTACCGGGGGGT‑T‑GG‑‑AA‑T‑GGGGGGGGGGGGGGTTTTTCCCCGC‑GTTGGTTTTTGGGGTGGTGGTTTTCGGTCTTTGGGGGGTTGTTGGCACAC‑CAAGCGGaaataaa                                                    >  2:99917/1‑149 (MQ=255)
                                                                                                     gatTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑C‑TAGGGC‑G‑TTTTTCCGGGGGTG‑T‑GG‑GAA‑A‑GGGGGGGGGGGGGGTTTTTCCCGGC‑GGTGAATTTTGGGGTGATGGTTTTTGATTTTGGTGCGGTTGGTGGCACAC‑AAGGGGAaaataaaaa                                                  >  2:179226/1‑148 (MQ=255)
                                                                                                     gatTGTGGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGC‑GGTTTTTCCGGGGGTC‑T‑GG‑CAA‑TGGGGGGGGGGGGGGTTTTTTCCCG‑C‑GTTGATTTTGGGGGTGGTGCTTTTCGGTCTTGGGGCGGTTTTTGGGACAC‑AAGGGGGaaataaa                                                    >  1:875558/1‑148 (MQ=255)
                                                                                                        tgtgGCGTGGATGCTGCTGGC‑GGG‑G‑AA‑A‑T‑GGGG‑GGTTTTTACGGGGGTC‑T‑TG‑GAA‑T‑GGGGGGGGGGGGGGTTTTTTCCCAC‑GGTTATTTTTGGGGTGGTGCTTTTCCGTCTTTGTGGGGTTGTTTGGACAA‑AAAGCGGAAATAAATATgg                                               >  1:1145439/1‑150 (MQ=255)
                                                                                                           ggCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTaa                                            >  1:1060584/1‑150 (MQ=255)
                                                                                                              gTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGAt                                        <  1:589614/151‑1 (MQ=255)
                                                                                                                 gATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACt                                     <  1:1080362/151‑1 (MQ=255)
                                                                                                                  aTGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTATTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACt                                     <  2:39682/150‑1 (MQ=255)
                                                                                                                    gctgctGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCa                                   <  2:179834/150‑1 (MQ=255)
                                                                                                                    gctgctGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCa                                   <  1:601264/150‑1 (MQ=255)
                                                                                                                     ctgctgGC‑GGG‑T‑AA‑A‑T‑GGGC‑GCTTTTAACGGGGGTC‑T‑GG‑CAA‑T‑GGGCGGGGGGGCGGTTTGTGCCGAC‑GTTTGTTCTGGTGGTGATGCTTATCGGTTTTGGTGCGTTTGTTGGCACAG‑AAGGCGAAAATAAATATGGTAAAGATACTCCgg                                 >  2:104237/1‑151 (MQ=255)
                                                                                                                       gctgGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGa                                <  2:770984/150‑1 (MQ=255)
                                                                                                                         tgGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGAtgt                             >  2:630976/1‑151 (MQ=255)
                                                                                                                          ggC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGAtgtg                            >  1:90403/1‑151 (MQ=255)
                                                                                                                              ggg‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGa                           >  2:1179654/1‑149 (MQ=255)
                                                                                                                              ggg‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAg                         <  1:985622/151‑1 (MQ=255)
                                                                                                                                  t‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGtat                      >  1:1233887/1‑151 (MQ=255)
                                                                                                                                    aa‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGtata                     <  2:685306/151‑1 (MQ=255)
                                                                                                                                       c‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATaa                    >  1:1105015/1‑150 (MQ=255)
                                                                                                                                         t‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATaa                    >  2:125832/1‑149 (MQ=255)
                                                                                                                                         t‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATaa                    >  1:24697/1‑149 (MQ=255)
                                                                                                                                           gggC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAg                  <  2:487459/150‑1 (MQ=255)
                                                                                                                                            ggC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGcc                >  1:318941/1‑151 (MQ=255)
                                                                                                                                                   tttACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACaa           <  2:1231049/150‑1 (MQ=255)
                                                                                                                                                    ttACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAAt          <  1:3951/150‑1 (MQ=255)
                                                                                                                                                        cGGGTGTC‑T‑GG‑CAG‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAAt      <  1:590173/150‑1 (MQ=255)
                                                                                                                                                           gtgtC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAg  <  2:750457/151‑1 (MQ=255)
                                                                                                                                                           gtgtC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAg  <  1:397437/151‑1 (MQ=255)
                                                                                                                                                            |                                                                                                                                                            
ACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGG‑T‑AA‑C‑T‑GGGC‑GATTTTACCGGGTGTC‑T‑GG‑CAA‑T‑GGGCGGTGGGGCGTTTTGTCCCGAC‑GTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACAC‑AAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAG  >  NC_000913/4112450‑4112748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑