Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 501,575 | 0 | A | C | 55.2% | 67.5 / 93.4 | 58 | V555G (GTG→GGG) | ybaL | inner membrane putative NAD(P)‑binding transporter |
Reads supporting (aligned to +/- strand): ref base A (24/2); new base C (0/32); total (24/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.53e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-11 |
AAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGG > NC_000913/501428‑501720 | aaGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCaccacc > 2:333860/1‑150 (MQ=255) aaGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCt > 1:747652/1‑151 (MQ=255) cgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACccgc > 2:547490/1‑151 (MQ=255) ggCCAGGTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCCCCGGTGGCGTTTc > 2:963890/1‑151 (MQ=255) aGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc > 2:351460/1‑151 (MQ=255) tAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGt > 2:1002744/1‑151 (MQ=255) tccccgccgcATCTGGCTTTTGCCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATCCCTACATTTTTTTCATATTTTACACCCGGCAC‑CCCCCGTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCa < 1:182538/144‑1 (MQ=37) ccgccgcATTGGGTATTTGCCTCCATCTGCCTGATGCAGCGCGGCCGCATCTTATCATGCCACCATTTTTTTTAT‑TTTTACCTCCGGGAACCCACGTTTTCCCCCGTCCCCCCCACACCCGCCGGTGGCGTTTCCAGCAGTTcc < 2:520907/143‑1 (MQ=255) aCGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg > 2:241986/1‑151 (MQ=255) aCGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg > 1:811759/1‑151 (MQ=255) ccgttgcTGGCATTTCTCTCTCGATACCGGTTGCGACGGTGGCGGGTCTTAACAAGCCTCCATATTTTACATATTTTTCATCCGGCAA‑CCTCCTTTTCCCCCGTCCCCCCCTCTCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt < 1:1198479/143‑1 (MQ=255) ccgcATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCTTCTTATCATGCCTACTTTTTTTTCATTTTTTACATCCGGCAA‑CCCCCTTTTCCCCCGTCACCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTAc < 1:447647/151‑1 (MQ=38) cATCTGTCTTTTGTCCCTCGTCGCCGGAGGCGCCGCCGGCGCGTTTTCTCGTCCCTAATTTTTTTTCATTTTTTTCATCCGCCAA‑CCACTGTTGCCCCTGCCGCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg < 2:1201909/145‑1 (MQ=255) tCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCTTTTTATCATCCCTACATTTTTTTCATTTTTTCCACCCGGCAA‑CCCCCTTTTACCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg < 2:1170954/149‑1 (MQ=255) cTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTAACCCGTCACCACCTCACCCGCCGGCGGCGTTTCCAGCAGATCCAGCATGGTACGGGCg > 2:792507/1‑150 (MQ=255) tGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt > 1:362765/1‑151 (MQ=255) ggCTTTTGGCTCTCTATCCTGGATGCGACGCTGGCCCGTTTTATCATGCCTACTTTTTTTCCATTTTTTACACCCGCCAC‑CCCCCGTTACCCCCTCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt < 2:79697/150‑1 (MQ=255) ggCTTTTGGCTCTCGATGCCTGATGCGACGCGGGCGCTTCTTATCATGCCTACATATTTTTCATATTTTACACCCGGCAC‑CCACCTTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt < 1:845602/150‑1 (MQ=255) ggCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt > 1:1224315/1‑151 (MQ=255) tttGTCTCCCGATGCCTTACGCGACGCGGGCGCGTCTTATCTGCCCTACATATTTTTAATATTTTACACCCGCCAA‑CCACCGTTTCCCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAttt < 1:1025726/148‑1 (MQ=255) tttGGCTCTCGTTGCCTGATGCGCCGCGGGCGCGTCTTATCATGCCTACATTTTTTTCATTTTTTCCACCCGGCAC‑CCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCa < 1:125289/150‑1 (MQ=255) tttGGCTCTCGATGCCTGATGCGCCGCGGGCGCTTCTTTTCATCCCTACATTTTTTTCATTTTTTACACCCGGCAC‑CCCCCTTTTCCCCCGCCCCCCCCGCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc < 2:11358/149‑1 (MQ=255) tcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGccc > 2:963978/1‑151 (MQ=255) ccTGATGCGACGCGGGCGCGTTTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCCCCTTTTCCCCCGCCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCAc < 1:345796/150‑1 (MQ=255) tGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc > 1:893082/1‑151 (MQ=255) gCGACGCGGGGGGTTTTTATCATGCCTACATATTTTTCATATTTTACACCCGGCAC‑CCCCCGTTTCCCCCGCCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTg < 2:793154/150‑1 (MQ=255) gACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt > 1:1128184/1‑151 (MQ=255) ggCGCTTTTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCAA‑CCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGc < 1:541485/148‑1 (MQ=255) ggCGCGTTTTATCATCCCTACTTTTTTTTAATTTTTTACACCCGCCAC‑CCCCCTTTTACCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcac < 2:730483/150‑1 (MQ=255) cgctttttATCATGCCTACATATTTTTAATTTTTTCCATCCGCCAA‑CCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacca < 1:582016/144‑1 (MQ=255) tATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGt > 1:743163/1‑151 (MQ=255) tATCATGCCTACATATTTTTCATATTTTACACCCGGCAC‑CCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTc < 1:862260/148‑1 (MQ=255) tATCATGCCTACATATTTTTCATATTTTACACCCGGCAC‑CCCCCGTTTCCCCCGTCCCCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTc < 2:468504/148‑1 (MQ=255) aTCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg > 1:39453/1‑151 (MQ=255) tCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg > 1:8469/1‑150 (MQ=255) tCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg > 1:534636/1‑150 (MQ=255) cATGCCTACATTTTTTTCATATTTTCCACCCGGCAC‑CCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGt < 1:918004/148‑1 (MQ=255) ccTACATTTTTTTCATTTTTTACATCCGGCAC‑CCACCTTTTACCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGata < 2:656535/148‑1 (MQ=255) aCATTTTTTTCATATTTTACACCCGACAA‑CCCCCGTTTCCCCCTTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc < 1:364875/148‑1 (MQ=255) cATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc > 2:582377/1‑150 (MQ=255) tttttCATTTTTTACCTCGGGCAA‑CCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACg < 1:911214/144‑1 (MQ=255) tttttCATATTTTCCATCCGGCAA‑CCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc < 1:675793/146‑1 (MQ=255) ttctttttttccccccGGCAC‑CCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCAATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCAt < 2:188934/136‑1 (MQ=255) atatTTTACATCCGCCAC‑CCCCCTTTCCCCCCGCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGt < 1:1092294/150‑1 (MQ=255) tatTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTc > 1:537697/1‑150 (MQ=255) ttttACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt > 2:881282/1‑150 (MQ=255) tttaccacccGCCAC‑CCACCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTc < 2:11179/140‑1 (MQ=255) tttACCCCCGGCAC‑CCCCCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATa < 2:288781/150‑1 (MQ=255) tACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt > 2:174722/1‑150 (MQ=255) tACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg > 1:730805/1‑151 (MQ=255) tACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg > 2:278265/1‑151 (MQ=255) ccGGCAC‑CCCCCTTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcgc < 1:915026/151‑1 (MQ=255) ccGCCAC‑CCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcg < 1:327015/150‑1 (MQ=255) cGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGcgcg > 1:539700/1‑151 (MQ=255) caccccccttttCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGcgcg < 2:741004/139‑1 (MQ=255) accccccttttCCCCCATCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGc < 2:951021/142‑1 (MQ=255) ccccGTTTCCCCCGCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaata < 2:202919/149‑1 (MQ=255) ccccGACCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGTGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCaa < 2:76262/149‑1 (MQ=255) cGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATcc < 2:502055/151‑1 (MQ=255) caccacCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCgg > 1:221516/1‑150 (MQ=255) | AAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAA‑CCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGG > NC_000913/501428‑501720 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |