Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,736,784	0	T	C	41.3%	70.1 / 10.6	46	G169G (GGA→GGG)	ydhP	putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (22/5);  new base C (0/19);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.06e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGC  >  NC_000913/1736635‑1736932                                                                                                                                                        |                                                                                                                                                     gTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTc                                                                                                                                                     <  1:883667/151‑1 (MQ=255) gTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTc                                                                                                                                                     >  2:189780/1‑151 (MQ=255)    aaCATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCa                                                                                                                                                  <  2:19205/151‑1 (MQ=255)     aCATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAg                                                                                                                                                 <  1:167201/151‑1 (MQ=255)        ttGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGccc                                                                                                                                              >  1:1090765/1‑151 (MQ=255)           cACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCccgc                                                                                                                                           >  2:401207/1‑151 (MQ=255)            aCCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCccgcc                                                                                                                                          >  1:1129165/1‑151 (MQ=255)             ccAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCccgcc                                                                                                                                          <  2:691415/150‑1 (MQ=255)                gCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGACAGCTCTTTTTTCACTTCAGGTCGCGCACCCGCACCACCTTTAGGACAT‑GAGAAGAAAAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGt                                                                                                                                        >  1:800622/1‑149 (MQ=255)                      aGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGa                                                                                                                                 >  1:1067058/1‑150 (MQ=255)                      aGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGa                                                                                                                                 >  2:742327/1‑150 (MQ=255)                                  agaaaTGCAGACAGCCCCGGCGGCCGCATTAATCCCGCCAGCTTTTTTTTCCCTTCAGGTCGTGCCCCTCCCCCCCCTTTAGGTAAT‑GAGAAGAAAAGATTTCCCATGAAAAT‑CACCCCCAGCCCCGCCGTTGCCAGAAATGAc                                                                                                                           <  2:804895/141‑1 (MQ=255)                                     aagccAGACACCCCCGGGGGCCGCATTAATCCCGCCAGCTTTTTTTTCCCTCCGGGTCGTCCCCCTCCCCCCCTTTTAGGTAAT‑GAAAAAAAAAGCCTTCCCATTAAAAC‑CCCCCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGc                                                                                                                 <  2:62792/147‑1 (MQ=255)                                     aaTGCAGACGCCCCCGGGGGACGCATTAATCCCGCCAGCTTTTTTTTCACTTCGGGTCGTCCCCCTGCCCCCCTTTTAGGTAAT‑GAGAAGAACAGACTTCCCATTAAAAT‑CCCCCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGc                                                                                                                 <  2:1061160/151‑1 (MQ=255)                                          aGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATg                                                                                                            >  2:146772/1‑151 (MQ=255)                                            aCAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGt                                                                                                          >  2:529279/1‑151 (MQ=255)                                             cccccccggCGGACGCTTAAATCCCGCCATCTTTTTTTTCACTTCAGGTCTTCCCCCTCCCCCCCTTTGAGGTAAT‑AAAAAGAAAAGTCTTACCATTGAAAC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGcc                                                                                                                <  1:661113/136‑1 (MQ=255)                                              aGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGttt                                                                                                        >  2:1057162/1‑151 (MQ=255)                                                ccccTGCGGACGCATTAATCCCGCCAGCTTTTTTTTCCCTTCAGGTCGTGCCCCTCCACCCCCTTTAGGTAAT‑GAGAAAAACAGCCTTACCATTAAAAC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGttt                                                                                                        <  2:852265/147‑1 (MQ=255)                                                          cGCATAAATCCCCCCACCTTTTTTTTCCCTTCAGGTCGCGCCCCTCCCCCCCTTTTAGAAAAT‑GAAAAAAAAAGACTTCCCATTAAAAC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAg                                                                                             <  1:856358/150‑1 (MQ=255)                                                                       ccgccttttttttCCCTGCAGGTCTTGCCCGTCCCCCCCTTTTAGGTAAG‑GGAAAAAACAATCTTGCCATAGAATC‑CCCTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCgccgcc                                                                                    <  2:448957/139‑1 (MQ=255)                                                                              ttttttCCCTTCTGTTCTGCCCCCTCCCCCCCTTTTAGTTAAT‑GAAAAAAACAGATTACCCATTAAACC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCAcc                                                                         <  1:602662/150‑1 (MQ=255)                                                                                ttttCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGat                                                                      >  2:542062/1‑151 (MQ=255)                                                                                 tttCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGat                                                                      >  2:237846/1‑150 (MQ=255)                                                                                  ttccttccgggTCGCGCCCCTCCCCCCCTTTTAGTAAAG‑GAAAAAAACAGACTTCCCA‑TGAAATCCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGata                                                                     <  2:1159661/141‑1 (MQ=255)                                                                                  ttCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGatat                                                                    >  1:365251/1‑151 (MQ=255)                                                                                  tcccttccgggTCGTCCCCCCCCCCCCCTTTAAGTTAAT‑GAAAAAAACAAACTTCCCATGAAACC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGata                                                                     <  1:958237/141‑1 (MQ=255)                                                                                     aCTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTg                                                                 >  1:140858/1‑151 (MQ=255)                                                                                      cTTCAGGTCG‑CCCCCCGCCCCCTCTTTTGGTAATGGAGAAAAAAAGCTTTCCCTTTAAACC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATAtt                                                                   <  1:712996/148‑1 (MQ=255)                                                                                         cAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGcc                                                               >  2:577609/1‑149 (MQ=255)                                                                                          aGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAgg                                                            >  2:307548/1‑151 (MQ=255)                                                                                           ggTCGTCCCCCGCCCCCCCTTTTAGGTAAG‑GAAAAAAACAGCCTTCCCATTAAAAC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAgg                                                            <  2:995709/150‑1 (MQ=255)                                                                                                       cccccccttttAGAAAAG‑GAAAAAAAAAGCCTTCCCATGAAAAC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCAt                                                 <  1:592870/141‑1 (MQ=255)                                                                                                          cccccTTTAGGTAAT‑GAAAAGAACAGACTTACCATTAAAAC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAAc                                             <  2:1104547/147‑1 (MQ=255)                                                                                                           cactTTTAGGTAAG‑GAGAAGAACAGACTTCCCATTAAAAC‑CACCCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACAt                                           <  2:279518/151‑1 (MQ=255)                                                                                                           caccTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACAt                                           >  1:1043164/1‑151 (MQ=255)                                                                                                               tttAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTg                                       >  2:1108472/1‑151 (MQ=255)                                                                                                                taagttAAT‑GAGAAGAACAGCTTTCCCATTGAATC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTg                                       <  2:507269/145‑1 (MQ=255)                                                                                                                 tAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGc                                     >  1:626029/1‑151 (MQ=255)                                                                                                                   gttAAT‑GAAAAGAACAGACTTCCCATTAAAAT‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGc                                     <  2:788505/147‑1 (MQ=255)                                                                                                                      aaT‑GAAAAAAACAGACTTCCCTTTGAAAC‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCa                                    <  2:265987/147‑1 (MQ=255)                                                                                                                       aataaaaaaaaCAGACTTCCCATTAAAAT‑CCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACt                                 <  2:1110168/141‑1 (MQ=255)                                                                                                                                     aCTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGttt                   >  1:674050/1‑150 (MQ=255)                                                                                                                                       ttACCATTAAAAC‑CACCCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGt                     <  2:124615/146‑1 (MQ=255)                                                                                                                                                      aCTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGc  >  1:439353/1‑151 (MQ=255)                                                                                                                                                      aCTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGc  >  1:385524/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                     GTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAAT‑GAGAAGAACAGACTTACCATTGAAAT‑CACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGC  >  NC_000913/1736635‑1736932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑