| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,825,061 | 0 | G | A | 41.3% | 67.2 / 25.1 | 46 | intergenic (‑124/+79) | ves/spy | cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced |
| Reads supporting (aligned to +/- strand): ref base G (26/1); new base A (0/19); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTA > NC_000913/1824915‑1825206 | ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGagaaaag‑aaa > 2:392069/1‑150 (MQ=255) tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAAc > 2:568532/1‑151 (MQ=255) tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAAc > 2:934791/1‑151 (MQ=255) tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGCGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAAc > 2:1182348/1‑151 (MQ=255) cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAAccg < 2:322126/151‑1 (MQ=255) cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAAcc > 2:1105099/1‑150 (MQ=255) cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAAccgcc > 1:695928/1‑151 (MQ=255) atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGAt > 1:1066561/1‑151 (MQ=255) cAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATcc > 1:944233/1‑151 (MQ=255) tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTcc > 2:73531/1‑151 (MQ=255) tccTCGCTCCGGCCCCTTTTTTTTAATTTTTATCGTTCAGGAACCCGTGGATGTTTCCCGCCAGCGGGGTTTATTTACTTTAGGGGTGATCGGGTTTTTTTTACAAC‑TGTTGA‑AGAAAAAAAAAA‑AAAACCGCCGATCCTGTCCAcc < 2:332099/145‑1 (MQ=255) cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGc > 1:1153754/1‑151 (MQ=255) tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGc > 1:706821/1‑150 (MQ=255) cccGGCCCTTTCTTTTCTTTTTTATCGTTCAGGTACCCGTGGATGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAAT‑TTTTTG‑AGAAGAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCa < 2:935575/150‑1 (MQ=255) gccccTTTTTTTTTTTTTTTATCTTTCGGGTAACGGGGGTTGTTTATCGGCACCTGGGGTATTTTACTTTAGGGGTGACCGGGTTTTTTTTACACT‑TGTTTA‑AGAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAg < 2:405370/147‑1 (MQ=255) ccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt > 1:113595/1‑151 (MQ=255) ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg > 2:57282/1‑150 (MQ=255) cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc > 2:193893/1‑150 (MQ=255) ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa > 1:1142290/1‑150 (MQ=255) tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg > 1:291420/1‑150 (MQ=255) gTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt > 1:504948/1‑150 (MQ=255) agttatcggTGGTTGTTTACCGGCATCTGGGGTGTTTTACGTTAGTGGTGACCTTTCTTTTTTAACAAT‑GGTTTG‑AGAAGAAAAAAA‑AAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt < 2:1057719/143‑1 (MQ=255) aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGAGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAggt > 1:560294/1‑149 (MQ=255) tAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt > 1:361649/1‑151 (MQ=255) gggtgtttATCGACACCTGGGGTTTTTTAGGTTAGTGTTGATCAGTTTTTTTTTACACT‑TTTTTG‑AGAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTg < 1:650529/140‑1 (MQ=255) aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa > 2:1003987/1‑150 (MQ=255) ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt > 2:550183/1‑151 (MQ=255) acctggggTGTTTTACGTTAGTGGTGATAAGGTTTTTTTTACAAC‑TGTTTG‑AAAAGAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca < 2:373430/146‑1 (MQ=255) cGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt > 1:979688/1‑150 (MQ=255) gggTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt > 1:262830/1‑149 (MQ=255) tttcctttagggtggATCAGTTTTTTTTAAAAAC‑TGTTGA‑AGAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg < 1:15744/133‑1 (MQ=255) tttACGTTAGGGGTGATCAGGTTTTTTTTACATC‑TTTTGG‑AAAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa < 1:405695/146‑1 (MQ=255) ttgggggtgATCAGGTTTTTTTTAAACC‑TGTTGG‑AAAAGAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa < 1:12206/144‑1 (MQ=255) ttAGTGGTGATCAGTTTTTTTTTACAAC‑TGTTGG‑AAAAGAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt < 1:376780/148‑1 (MQ=255) aggggttatcggcttttttttCCACC‑TGTTAA‑AAAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt < 2:1059521/132‑1 (MQ=255) gtggtgATCGGTTTTTTTTTACAAC‑TTTTGG‑AGAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt < 1:577246/149‑1 (MQ=255) gtggtgATCAGTTTTTTTTTACATC‑TTTTGG‑AAAAGAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt < 1:515335/151‑1 (MQ=255) ggtaatcggttttttttACCACTTGTTTG‑AGAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt < 1:568530/137‑1 (MQ=255) tgATCGGGTTTTTTTTACAAT‑TTTTGG‑AAAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg < 1:264295/149‑1 (MQ=255) cAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc > 1:434152/1‑149 (MQ=255) ttttttttACATT‑TTTTGG‑AAAAGAAAAAAA‑AAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAc < 2:317219/149‑1 (MQ=255) ttttttAACAC‑TTTTTGAAAAAAAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAc < 2:975841/149‑1 (MQ=255) ttACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt > 1:975598/1‑151 (MQ=255) aaC‑TTTTGG‑AAAAGAAAAAAA‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg < 1:392068/150‑1 (MQ=255) tGTTTG‑AGAAGAAAAAAA‑AAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg < 1:354166/150‑1 (MQ=255) ttG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCCGGACGGTCTGTCAGACGCtt > 2:1085534/1‑150 (MQ=255) agaaaaaagaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa < 1:217104/149‑1 (MQ=255) gagaaAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaaa > 1:1092367/1‑150 (MQ=255) aaaaaa‑aaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTa < 1:627413/150‑1 (MQ=255) | TTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAC‑TGTTTG‑AGAAGAGAAAAG‑AAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTA > NC_000913/1824915‑1825206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |