Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,547,7720TG41.9% 128.4 / 12.7 75V367G (GTA→GGA) murPN‑acetylmuramic acid permease, EIIBC component, PTS system
Reads supporting (aligned to +/- strand):  ref base T (13/30);  new base G (31/0);  total (45/30)
Fisher's exact test for biased strand distribution p-value = 1.24e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCT  >  NC_000913/2547625‑2547919
                                                                                                                                                    |                                                                                                                                                    
gCTTTATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGGAc                                                                                                                                                    >  2:154974/1‑150 (MQ=255)
  tttATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCCCAATGCGCCACGCAGCCAGGGAcgc                                                                                                                                                  >  1:9313/1‑150 (MQ=255)
   ttATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCCCAGTGCGCTACGCAGGCAGGGACGCgg                                                                                                                                                >  2:681206/1‑151 (MQ=255)
    tATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCAACCCCACAGTGCGCTACGCAGGCCGGGACGCggg                                                                                                                                               >  1:363627/1‑151 (MQ=255)
     aTTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCgggg                                                                                                                                              <  1:435031/151‑1 (MQ=255)
     aTTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCggg                                                                                                                                               <  2:302343/150‑1 (MQ=255)
      ttCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCACAGTGCGCTACGCCGTCAGGGACGCgggg                                                                                                                                              >  1:594915/1‑150 (MQ=255)
       tCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCAACCCCACAGGGCGCCACGCAGGCAGGGAAGCgggg                                                                                                                                              >  2:525180/1‑149 (MQ=255)
       tCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGc                                                                                                                                             <  2:149884/150‑1 (MQ=255)
             ttACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCGACCCCACACGGCGCTACGCAGTCAGGTGGGCggggggggtatt                                                                                                                                      >  2:1148534/1‑143 (MQ=255)
             ttACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGattat                                                                                                                                      <  2:905910/150‑1 (MQ=255)
             ttACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGattat                                                                                                                                      <  2:332053/150‑1 (MQ=255)
               aCCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTc                                                                                                                                    <  1:274636/150‑1 (MQ=255)
                ccTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGAGCGCCACGCAGTCAGGTACGCGGGGCGATTATTccc                                                                                                                                  >  2:614010/1‑151 (MQ=255)
                      gTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGGCAGGGACGCGGGGCGGTTATTTCCGGcc                                                                                                                              >  2:125741/1‑149 (MQ=255)
                      gTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCCCAGAGCGCCAAGCAGCCCGGGACGCGGGGCGGTTATTTCCCGcccg                                                                                                                            >  2:848763/1‑149 (MQ=255)
                         aaTGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCctgctg                                                                                                                         <  2:893095/151‑1 (MQ=255)
                         aaTGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCctgctg                                                                                                                         <  2:420457/151‑1 (MQ=255)
                          aTGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCCCAAGGCGCTACGCAGTCAGGGCCGCGGGGGGGTTATTTCCGGccccccgg                                                                                                                        >  1:330602/1‑145 (MQ=255)
                           tGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCCCAGTGGGCTACGCAGTCAGGTACGCGGGGCGGTTATTCCCGGCCTGCTggg                                                                                                                       >  2:702846/1‑151 (MQ=255)
                              aCAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCCCAGTGGGCTCCGCCGGCCGGGACGCGGGGCGGTTTTTTCCGGCCTGCCggg                                                                                                                       >  1:236627/1‑148 (MQ=255)
                              aCAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCCACGCAGTCAGGTACGCGGGGGGGTTATTCCCGGCCCGCTggg                                                                                                                       >  2:400173/1‑148 (MQ=255)
                               cAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCACAAGGCGCTACGCCGTCAGGGACGCGGGGCGGTTATTTCCCGCCCGCCggg                                                                                                                       >  2:363563/1‑147 (MQ=255)
                                 gCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCCCACGGCGCCACGCCGGCAGGCACGCCGGGGGGTTTTTTCCGGCCTGCCGGGGGGTgg                                                                                                                 >  1:691174/1‑151 (MQ=255)
                                   cAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGAGCGCTACCCAGTCAGGGACGCGGGGGGGTTTTTTCCGGGCTGCTGGGCGgtggtg                                                                                                               >  2:250134/1‑151 (MQ=255)
                                    aGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGCCAAACCCACCCAGCGCCACGCCGTCCAGGACGCGGGGGGGTTATTCTCCGGCTCCCGGGCGCTGGTg                                                                                                               >  1:266297/1‑150 (MQ=255)
                                    aGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGa                                                                                                              <  1:874564/151‑1 (MQ=255)
                                        aTTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGGACGCGGGGGGAATATTTCCGGCCCGCTGGGGGGTGGTGAAcc                                                                                                           >  2:173071/1‑150 (MQ=255)
                                            aaCAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCCACCGCACAGAGCGCCACCCCGTCAGGGCCGCGGGGGGGTTATTTCCCGCCTGCTGGGGGGTGGTGAAcc                                                                                                           >  2:933151/1‑146 (MQ=255)
                                             aCAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGGACGCGGGGGGATTTTTCCCGGCCCGCTTGGCGTTGGTGGACCGCTg                                                                                                       >  2:1018279/1‑149 (MQ=255)
                                              cAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCCCCCAGTGCGCCACGCAGGCCGGGACGCGGGGGGGTTATTTCCGGCCCGCCGGGCGGTGGGGGACCGccggtt                                                                                                    >  1:807751/1‑146 (MQ=255)
                                              cAGCTTATTTCCCATCCGTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGAt                                                                                                     <  2:302951/150‑1 (MQ=255)
                                                       tCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGGGCGCTACGCAGTCAGGGACGCGGGGCGGTTATTCCCGGCCCGCTGGGGGTTGGTGGACCGCTGGTTTTCGgtgt                                                                                            >  2:275214/1‑150 (MQ=255)
                                                        cccATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGGCAACCCCAAGAGCGCTTCGCAGGCAGGGACGCGGGGGGGTTATTCCCCGCCCGCTGGGGGTTGGTGGACCGCCGAgtttctg                                                                                               >  1:441175/1‑142 (MQ=255)
                                                         ccATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCAc                                                                                         <  2:726366/151‑1 (MQ=255)
                                                          cATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGGGCCACGCCGTCAGGGACGCGGGGGGGTTTTTTCCGCCCCGCTGGGGGGTGGGGAAACCCTGGTTTACGGTGTCAcc                                                                                        >  1:551242/1‑151 (MQ=255)
                                                             ccTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTg                                                                                     <  1:16050/151‑1 (MQ=255)
                                                             ccTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTg                                                                                     <  1:634731/151‑1 (MQ=255)
                                                             ccTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCCACGCCGGCAGGGACGCGGGGGGATTTTTTCCCGCCTGCTGGGGGTTGGGGGACCCCTGATTTTTGGGGTTAccc                                                                                       >  1:1072699/1‑149 (MQ=255)
                                                             ccTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAAACGCACCGTGGGCTACGCAGGCCGGTACGCGGGGCGGTTATTTCCGGCCGGCTGGGGGTTGGTGGACCGCTGTTTTACGGGGTCGccc                                                                                       >  1:771528/1‑149 (MQ=255)
                                                                ttCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGcc                                                                                   <  1:344171/150‑1 (MQ=255)
                                                                 tCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCCCCCAGGGCGCTACGCCGTCAGGGACGCGGGGCGGTTATTCCCGGCCTGCTGGGCGCTGGTGAACCGCTGATTTTCGGTGTCACCCTCcccc                                                                                 >  2:703163/1‑151 (MQ=255)
                                                                  cAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCCACCCCCCAGGGCGCTACGCAGTCAGGGACGCGGGGGGGGTATTTCCGGCCTGCCGGGGGTTGGGGGAACGCTGATTTTCGGGGTCACCCCGcccc                                                                                 >  1:294518/1‑150 (MQ=255)
                                                                    aTGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATACCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGGCCCGCa                                                                              <  1:747853/151‑1 (MQ=255)
                                                                      ggCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCAt                                                                             <  2:123840/150‑1 (MQ=255)
                                                                      ggCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCAt                                                                             <  2:1121812/150‑1 (MQ=255)
                                                                         aGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGGGCAACCGCACAAGGGGCCACGCAGGCAGGGACGCGGGGGGGTTATTTCCCGGCCGCTGGGCGTTGGGGAAACGCCGGTTTTCGGGGTCACCCCCCCCCGcct                                                                             >  1:559462/1‑145 (MQ=255)
                                                                         aGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCCACCGCACCATGCGCTCCGCAGGCCGGGACGCGGGGCGGTTATTCCCCGCCCGCTGGGCGGTGGGGGACCGCCGGTTTTCgggggtcccccgcccc                                                                                 >  1:504006/1‑129 (MQ=255)
                                                                              cgGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGtt                                                                    <  1:76903/151‑1 (MQ=255)
                                                                              cgGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGGCAGGGACGCGGGGCGGTTATTTCCCGCCCGCCGGGCGGTGGTGGAACGCTGATTTTCGGGGTCCCCCCGCCCCCCCTGAAACCGtt                                                                    >  1:1076511/1‑151 (MQ=255)
                                                                                    aGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAATGCGCTACGCCGTCAGGGACGCGGGGCGGTTATTTCCCGCCCGCTGGGCGTTGGTGGAACGCTGGTTTACGGTGTCACCCTGCCCCCCCTGAAGCCGTTTGtt                                                                >  1:736948/1‑149 (MQ=255)
                                                                                            gcggcgcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGtt                                                      <  2:205938/151‑1 (MQ=255)
                                                                                              ggcgcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTa                                                    <  2:36718/151‑1 (MQ=255)
                                                                                              ggcgcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTa                                                    <  2:240877/151‑1 (MQ=255)
                                                                                              ggcgcTGGCACTCTACTGGCGGGCGCAAACGCACAGTGCGCTACGCAGTCAAGGACGCGGGGCGATTATTTCCGGCCCGCCGGGCGGTGGGGGACCGCCGGTTTACGGGGTCACACCGCCCCGCAAGAAACCGGTTTTTTCCGcccggt                                                      >  2:12360/1‑145 (MQ=255)
                                                                                               gcgcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAg                                                   <  1:996930/151‑1 (MQ=255)
                                                                                                 gcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGGACGCGGGGGGGTTATTCCCCGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGGGTCACCCCGCCCCGCATGAAACCGTTTGTTTCCGCCTGttt                                                     >  2:39267/1‑147 (MQ=255)
                                                                                                  cTGGCACTCTACTGGCGGGCGCCACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTTTTTCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGGGTCACCCTGCCCCCCATGAAGCCGTTTGTTACCGCCCGTTTAggcg                                                >  2:531551/1‑151 (MQ=255)
                                                                                                      cACTCTACTGGCGGGCGCCACCGCCCAGTGCGCTACGCAGTCAGGGACGCGGGGCGGTTATTTCCCGCCCGCTGGGCGTTGGTGAACCGCCGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTTCCGCCTGTTTTggcgg                                               >  1:1185486/1‑148 (MQ=255)
                                                                                                      cACTCTACTGGCGGGCGCAACCGCCCAAGGCGCTACGCCGGCCGGGACCCGGGGGGGTTATTCCCCGCCCGCCGGGGGGTGGGGGAACGCTGGTTTTCGGTGTCACCCTGCCCCCCCTGAAGCCGGTTGTTACCGCCTGTTTTGggggcg                                             >  1:126140/1‑150 (MQ=255)
                                                                                                             cTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCggcggg                                      >  1:796805/1‑150 (MQ=255)
                                                                                                              tGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTGCGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGcggcgg                                       <  2:289859/148‑1 (MQ=255)
                                                                                                               ggcgggcgCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGCGGCGCGGCGGGCGgt                                  <  2:588938/151‑1 (MQ=255)
                                                                                                                   ggcgCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGgtttg                               >  2:457362/1‑151 (MQ=255)
                                                                                                                        cAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGgtttgttt                            >  1:485212/1‑150 (MQ=255)
                                                                                                                         aaCCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTAt                          >  1:1118801/1‑151 (MQ=255)
                                                                                                                           ccGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGGTTGTTTATCg                        >  1:1015541/1‑151 (MQ=255)
                                                                                                                            cGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCgg                       >  2:188020/1‑151 (MQ=255)
                                                                                                                             gCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGc                      <  2:202528/151‑1 (MQ=255)
                                                                                                                              cacaGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGcc                     <  1:1147563/151‑1 (MQ=255)
                                                                                                                                    gcgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATa                <  2:8641/150‑1 (MQ=255)
                                                                                                                                      gcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGcc             >  1:960815/1‑151 (MQ=255)
                                                                                                                                         aCGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtg           <  2:195924/150‑1 (MQ=255)
                                                                                                                                                cAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTc   <  1:1087531/151‑1 (MQ=255)
                                                                                                                                                 aGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCt  <  1:848758/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTTTATTCCTGTTTACCTCGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCT  >  NC_000913/2547625‑2547919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: