Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,133,302	0	T	G	46.2%	134.2 / 18.6	80	H219P (CAC→CCC)	yghS	putative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (4/39);  new base G (37/0);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.19e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGG  >  NC_000913/3133153‑3133450                                                                                                                                                      |                                                                                                                                                      aaTGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg                                                                                                                                                      <  2:626330/151‑1 (MQ=255)  aTGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtgt                                                                                                                                                     <  2:704630/151‑1 (MQ=255)      aGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGcc                                                                                                                                                 <  1:887264/151‑1 (MQ=255)      aGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGc                                                                                                                                                  >  1:1148049/1‑150 (MQ=255)              tGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGGCCCTCCCGt                                                                                                                                         >  1:1048233/1‑151 (MQ=255)                 ggcgCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCCTCCCGtt                                                                                                                                        >  2:570367/1‑149 (MQ=255)                    gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAgg                                                                                                                                   <  1:160581/151‑1 (MQ=255)                    gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAgg                                                                                                                                   <  2:1126448/151‑1 (MQ=255)                      ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGAt                                                                                                                                 <  1:315377/151‑1 (MQ=255)                      ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGAt                                                                                                                                 <  1:977793/151‑1 (MQ=255)                      ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGAt                                                                                                                                 <  2:390290/151‑1 (MQ=255)                      ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTcca                                                                                                                                     >  1:472884/1‑145 (MQ=255)                            aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTg                                                                                                                            <  2:1030938/150‑1 (MQ=255)                               aTTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCAAGGATCTTTTCGcc                                                                                                                        >  2:134253/1‑151 (MQ=255)                                 tAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCa                                                                                                                       <  1:852856/150‑1 (MQ=255)                                 tAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGAGTTTTTCGCCa                                                                                                                       >  1:597319/1‑150 (MQ=255)                                  aaCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCAAGGATCTTTTCGCCCtt                                                                                                                     >  1:992765/1‑151 (MQ=255)                                   aCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGATCTTTGCGCCCtt                                                                                                                     >  2:637636/1‑150 (MQ=255)                                   aCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGTCCATCCAGTTCAAGGATTTTTTCGCCAtt                                                                                                                     >  1:334556/1‑150 (MQ=255)                                    cAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAAGATCTTTTCGcc                                                                                                                        >  1:1527/1‑146 (MQ=255)                                      gtttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTTCAGGAATTTTTTGCCAtttta                                                                                                                  >  1:799826/1‑147 (MQ=255)                                       tttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGGTTCAGGGTCTTTTCGCCAtttgat                                                                                                                 >  1:245528/1‑146 (MQ=255)                                        ttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATg                                                                                                                <  2:575714/150‑1 (MQ=255)                                        ttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATg                                                                                                                <  1:1063049/150‑1 (MQ=255)                                         tgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGt                                                                                                               <  2:884308/150‑1 (MQ=255)                                         tgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGGTTACAGATCTTTGCGCCCTTgga                                                                                                                  >  1:214878/1‑145 (MQ=255)                                         tgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGGTCAAAGGTCTTTTCGCCCTTGaa                                                                                                                  >  2:75659/1‑147 (MQ=255)                                           agtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAg                                                                                                            <  1:1020721/151‑1 (MQ=255)                                               tggaGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCTCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTg                                                                                                         <  2:721965/147‑1 (MQ=255)                                                gTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgc                                                                                                        <  2:71422/150‑1 (MQ=255)                                                      cATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTaa                                                                                                 <  2:1053223/151‑1 (MQ=255)                                                      cATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTCGCCATTGAATGTCAGGTgcggcg                                                                                                    >  2:443722/1‑148 (MQ=255)                                                       aTCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTTAAGGATCTTTTCGCCATTTAATGTCAGTTGCGGCGCaa                                                                                                 >  1:242970/1‑150 (MQ=255)                                                        tCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGTTCAAGGATCTTTTCGCCCTTGAATGTCAGTTGCGGCGGaa                                                                                                 >  2:171315/1‑149 (MQ=255)                                                           aTTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCg                                                                                            >  2:1147173/1‑151 (MQ=255)                                                            ttCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGATCTTTTCGCCCTTGAATGTCAGTTGCGGCGTAAAGGCgg                                                                                           >  2:1139422/1‑150 (MQ=255)                                                             tCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCAAAGATCTTTTCGCCCTTGAATGTCAGTTGCGGCGGAACGGCggt                                                                                          >  2:19340/1‑149 (MQ=255)                                                               gTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAttt                                                                                        <  2:574414/151‑1 (MQ=255)                                                                  aaaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAtttttt                                                                                     <  1:1040283/151‑1 (MQ=255)                                                                   aaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTc                                                                                    <  2:627269/151‑1 (MQ=255)                                                                      catcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTg                                                                                 <  2:870240/151‑1 (MQ=255)                                                                      catcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCCCCCCTCCAGCTCTAAGGTCTTTTCGCCCTTGAAAGTGAGTTGCGGGCTAACAGCGCttttttt                                                                                    >  2:413984/1‑147 (MQ=255)                                                                       atcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTCGCCATTGAATGTCAGTTGCGGGGTAACGGGGATTTTTTTCTGt                                                                                >  2:1083027/1‑151 (MQ=255)                                                                           ccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCGGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACg                                                                            <  2:1074087/151‑1 (MQ=255)                                                                           ccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACg                                                                            <  1:688722/151‑1 (MQ=255)                                                                           ccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACg                                                                            <  2:540763/151‑1 (MQ=255)                                                                             tATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCACCCGTTCAAGGATCTTTGCGCCCTTGTATGGCAGGTTCGGGGTAAGGGGGAGTTTTTTCTGtga                                                                              >  2:663203/1‑145 (MQ=255)                                                                              aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTg                                                                         <  2:1033253/151‑1 (MQ=255)                                                                              aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGGTCTTTGCGCCCTTGAATGTCAGTTGCGGGGGAACGGCGATTTTTTTCTTTAAAGCt                                                                          >  1:1163545/1‑150 (MQ=255)                                                                                ccATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCCTCCCGTTCAAAGATCTTTTCGCCATTGTATGTCAGTTTCGGCGGAACGGCGGTTTTTTTCTTTTAAGCTG‑cc                                                                      >  1:110432/1‑151 (MQ=255)                                                                                    tttGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAAGTCCAGGAACTTTTCGCCCTTTTATGTCCGTTGCGGCGTAACCGCGGTTTTTTTCttttaatccgcccgtt                                                                   >  2:371363/1‑136 (MQ=255)                                                                                        gTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAATTCAAGGGACTTTGTGCCACTTCATGTCGGTTGCGGCGCAACGGCGAATTTTTTCTCTaaaaccgccgggtggt                                                                >  1:391176/1‑136 (MQ=255)                                                                                         tGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTCGCCCTTGAATGTCAGTTGCGGCGTAACGGCGGTTTTTTTCTGTAACGCTG‑CCCGTtggggg                                                             >  2:1142748/1‑151 (MQ=255)                                                                                           aTCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGGTCCAAGATCTTTTTGCCATTTTATGGCAGTTGCGGGGTAAAGGCGATTTTTTTCTTTAACGCCG‑CCAGTtggggg                                                             >  1:800056/1‑149 (MQ=255)                                                                                            tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTTGCCATTGAATGTCAGTTGCGGCGGAACGGCGGTTTTTTTCTGTAACGCTG‑CCAGTtgg                                                                >  2:561331/1‑145 (MQ=255)                                                                                             cAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAg                                                         <  2:321627/151‑1 (MQ=255)                                                                                               ggAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCCCCCATCCAGTTCCAGGATCTTTTCGCCATTGAATGTCAGTTGCGGCGGAACGGCGGTTTTTTCCTTTAAAGCTG‑CCCGGTGGGGGGCa                                                          >  2:582521/1‑148 (MQ=255)                                                                                                 aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGttt                                                     <  1:475147/151‑1 (MQ=255)                                                                                                  aaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCGAGGGGGTTTGCGCCATTTAATGTCCGTTGGGGCGGTACGGCGCTTTTTTTCTTTTACCCCG‑CCCGTTGGTGGGCacggt                                                      >  1:518701/1‑145 (MQ=255)                                                                                                          cgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGAACTTTGCGCCCTTTAATGTCAGGTGCGGCGTAACGGCGATTTTTTTCTGTAAAGCTG‑CCAGTTGGGGGGCTGGGTTTCGCGCaa                                             >  2:1013105/1‑150 (MQ=255)                                                                                                           gTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTGGCCCCTCCCGTTCTAGGATCTTTTTGCCATTGAATGTTAGGTTCGGGGTAACGGCGCTTTTTTTCTGTTAAGCCG‑CCAGTTGGTGGTGAGGGTTTTGCGCcaa                                            >  1:619126/1‑150 (MQ=255)                                                                                                           gTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAAc                                           <  2:245528/151‑1 (MQ=255)                                                                                                           gTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAAc                                           <  1:1187490/151‑1 (MQ=255)                                                                                                            tGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAAGGTCTTTGCGCCATTTAATGTCAGTTGCGGCGTAACGGCGATTTTTTTCTGTAACGCCG‑CCAGTTGGGGGGCAGGTTTTCGCGCaaa                                            >  1:342048/1‑149 (MQ=255)                                                                                                            tGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTTCGGCGGAACGGGGATTTTTTCCTGTAAAGCTG‑CCAGTTGGGGGGCGGGTTTTCGCGCaaa                                            >  1:103916/1‑149 (MQ=255)                                                                                                             gAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAAcg                                          <  1:40559/150‑1 (MQ=255)                                                                                                                  gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGTGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGttt                                    <  2:877703/151‑1 (MQ=255)                                                                                                                  gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTTGGGCGGAACGGCGGTTTTTTTCTTTAACGCCG‑CCAGATGGGGGGCAGGTTTTCGGGCAAACGCGGgtt                                    >  1:847525/1‑151 (MQ=255)                                                                                                                       tAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGGTTCAGGATCTTTTCGCCCTTGAATGTCAGGTGGGGGGTAACGGGGGTTTTTTTCTTTTACGCCGCCCGGTGGGGGGGCGGGTTTA‑GGGCAAAAGCGGgttgtt                                 >  1:272815/1‑149 (MQ=255)                                                                                                                        aGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtca                               <  1:1026929/150‑1 (MQ=255)                                                                                                                           gACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCaa                           <  1:344359/151‑1 (MQ=255)                                                                                                                             cGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATg                         <  1:367302/151‑1 (MQ=255)                                                                                                                              gCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGGTCTTTTCGCCATTGAATGTCAGTTGCGGGGGAACGGCGATTTTTTTCTTTTACGCTT‑CCCGTTGGGGGGCAGGGTTTCGCGCGAACGCGGGTTGTTCTTCaaag                         >  1:807749/1‑148 (MQ=255)                                                                                                                                ttGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGCTTAAGGATCCTTTTGCCATTGAATGTCAGTTGGGGCGTAACGGCGGTTTTTTTTTTTTACGCCG‑CCCGTTGGTGGGCCGGGTTACGCGCAAAAGCGGGTTTTTCTTCAATTcc                       >  1:434822/1‑150 (MQ=255)                                                                                                                                  gCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAg                    <  1:570365/151‑1 (MQ=255)                                                                                                                                     gAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACg                 <  1:1164432/151‑1 (MQ=255)                                                                                                                                       aTTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGaa               <  1:7439/151‑1 (MQ=255)                                                                                                                                          tCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATc             <  2:1048240/150‑1 (MQ=255)                                                                                                                                              cggcggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaaca         <  1:1109768/150‑1 (MQ=255)                                                                                                                                                gcggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATa      <  1:141550/151‑1 (MQ=255)                                                                                                                                                 cggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATAc     >  2:775600/1‑151 (MQ=255)                                                                                                                                                    ggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACggg  <  2:334557/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      AATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTG‑CCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGG  >  NC_000913/3133153‑3133450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑