Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,564,102 T→G L544F (TTA→TTC)  dosP ← oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,564,1020TG98.0% 168.6 / NA 49L544F (TTA→TTC) dosPoxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (18/30);  minor base A (0/1);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTGAAGGGGGCACATGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGT  >  NC_000913/1563955‑1564238
                                                                                                                                                   |                                                                                                                                        
ccGTGAAGGGGGCACATGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTCCAGCGCTGCGCCGaaa                                                                                                                                       <  2:470395/151‑1 (MQ=255)
  gTGAAGGGGGCACATGGCCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAAc                                                                                                                                     <  1:1094505/151‑1 (MQ=255)
  gTGAAGGGGGCACATGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAAc                                                                                                                                     >  1:647813/1‑151 (MQ=255)
    gAAGGGGGCACATGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCa                                                                                                                                   <  1:1112208/151‑1 (MQ=255)
    gAAGGGGGCACATGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCa                                                                                                                                   <  1:675065/151‑1 (MQ=255)
               aTGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCtttt                                                                                                                        <  1:30039/151‑1 (MQ=255)
                tGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCtttt                                                                                                                        >  1:1019555/1‑150 (MQ=255)
                    cATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCAt                                                                                                                   >  1:172651/1‑151 (MQ=255)
                     aTGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCAtt                                                                                                                  <  2:606488/151‑1 (MQ=255)
                        cAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTGCCATTTCg                                                                                                               <  2:252045/151‑1 (MQ=255)
                          ggggATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGtt                                                                                                             <  1:555561/151‑1 (MQ=255)
                           gggATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTc                                                                                                            <  1:815529/151‑1 (MQ=255)
                              aTCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATc                                                                                                         <  2:804863/151‑1 (MQ=255)
                                   gCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCg                                                                                                     <  2:1053157/150‑1 (MQ=255)
                                             aaGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAAc                                                                                          <  1:640895/151‑1 (MQ=255)
                                                ggCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGc                                                                                       >  1:778716/1‑151 (MQ=255)
                                                 gCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGcc                                                                                      >  1:304489/1‑151 (MQ=255)
                                                      gATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCg                                                                                 >  2:6645/1‑151 (MQ=255)
                                                      gATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCg                                                                                 <  1:419940/151‑1 (MQ=255)
                                                       aTGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGt                                                                                >  2:463262/1‑151 (MQ=255)
                                                         gCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGtt                                                                               <  1:451904/150‑1 (MQ=255)
                                                         gCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGtt                                                                               <  1:311200/150‑1 (MQ=255)
                                                         gCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGtt                                                                               >  1:943867/1‑150 (MQ=255)
                                                           cGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTAcc                                                                            <  2:403889/151‑1 (MQ=255)
                                                           cGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTAcc                                                                            <  1:214286/151‑1 (MQ=255)
                                                              aCAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGcc                                                                         >  2:235258/1‑151 (MQ=255)
                                                                      cccGTTTCTGCGAAGATTTGCGGCTGGTAACCCAGTTTCAGTTGTTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAACCCAACCGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAAAACTGCCAGCCGTTACCGCCATTCtt                                                                   <  1:1058357/149‑1 (MQ=255)
                                                                      cccGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTCCCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTg                                                                  <  1:496950/150‑1 (MQ=255)
                                                                       ccGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCg                                                                <  1:404824/151‑1 (MQ=255)
                                                                         gTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGa                                                               >  1:714040/1‑150 (MQ=255)
                                                                                cGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGAGAATATAATc                                                       >  1:1185893/1‑151 (MQ=255)
                                                                                              tGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATtgtg                                           >  1:951842/1‑149 (MQ=255)
                                                                                                  aaaCCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGt                                     >  2:126009/1‑151 (MQ=255)
                                                                                                    aCCAGTTTCAGTTGGTTATTCCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTCCCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTgg                                   <  1:419342/151‑1 (MQ=255)
                                                                                                       aGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTgg                                   >  1:840457/1‑148 (MQ=255)
                                                                                                        gTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAACCCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGgaga                                <  1:256397/150‑1 (MQ=255)
                                                                                                          ttCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGc                              >  2:581647/1‑150 (MQ=255)
                                                                                                          ttCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGc                              >  1:1176984/1‑150 (MQ=255)
                                                                                                             aGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCaa                            <  2:229537/149‑1 (MQ=255)
                                                                                                              gTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCTTTCATCGCCGGGCTGAAGAACGGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCaa                            <  2:1119517/148‑1 (MQ=255)
                                                                                                                      ttGCTAATCGCTTCTTTCAGCGCTGCGCCAAAACCCAAACGCTCTTTTCCCATTTCTTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCgg                  <  1:648182/150‑1 (MQ=255)
                                                                                                                         cTAATCGCTTCTTTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt              >  1:727581/1‑151 (MQ=255)
                                                                                                                            aTCGCTTCTTTCAGCGCTGCGCCGAAAACAAAACGCTCTTTTACCATTTCTTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt              <  2:409785/148‑1 (MQ=255)
                                                                                                                                 ttcttTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCTTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTAccc         <  1:323657/148‑1 (MQ=255)
                                                                                                                                  tcttTCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAgg      >  1:1141107/1‑150 (MQ=255)
                                                                                                                                     ttCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCg   <  1:6645/150‑1 (MQ=255)
                                                                                                                                      tCAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGt  <  2:372881/150‑1 (MQ=255)
                                                                                                                                       cAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGt  <  2:647816/149‑1 (MQ=255)
                                                                                                                                       cAGCGCTGCGCCGAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGt  <  2:291113/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
CCGTGAAGGGGGCACATGACCATGCAGGGGATCGTGCCAGCGAGCAAGGGCTTCGATGCCGTACAGTTCACCCGTTTCTGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGT  >  NC_000913/1563955‑1564238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑