Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,536,332 +T coding (253/720 nt) ompR ← response regulator in two‑component regulatory system with EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,536,3291.T99.0% 409.4 / ‑3.2 103coding (256/720 nt)ompRresponse regulator in two‑component regulatory system with EnvZ
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (51/51);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 111 total aligned reads displayed.

CCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTG  >  NC_000913/3536179‑3536475
                                                                                                                                                       |                                                                                                                                                   
ccTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTcccc                                                                                                                                                      <  2:205866/151‑1 (MQ=255)
     tCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCtttt                                                                                                                                                <  1:674426/150‑2 (MQ=255)
         gtgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCg                                                                                                                                            <  1:1197061/151‑1 (MQ=255)
         gtgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCttttcgc                                                                                                                                             <  2:1061245/149‑5 (MQ=255)
          tgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCg                                                                                                                                            >  2:526985/1‑150 (MQ=255)
          tgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGt                                                                                                                                           >  1:43475/1‑151 (MQ=255)
          tgACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGt                                                                                                                                           >  1:376679/1‑151 (MQ=255)
           gACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGt                                                                                                                                           <  2:678446/150‑1 (MQ=255)
            aCGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCa                                                                                                                                         <  2:907346/151‑1 (MQ=255)
                  cgcCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATa                                                                                                                                    >  2:858611/1‑150 (MQ=255)
                   gcCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAAt                                                                                                                                  >  2:386120/1‑151 (MQ=255)
                     cTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATg                                                                                                                                 >  2:263137/1‑150 (MQ=255)
                     cTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATg                                                                                                                                 >  1:807631/1‑150 (MQ=255)
                      tGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGAt                                                                                                                               >  1:43864/1‑151 (MQ=255)
                       ggCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGAt                                                                                                                               <  1:490201/150‑1 (MQ=255)
                       ggCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGAt                                                                                                                               <  2:1059448/150‑1 (MQ=255)
                         cAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCgg                                                                                                                            <  2:1333808/151‑1 (MQ=255)
                            ttcgttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCAt                                                                                                                         >  2:148023/1‑151 (MQ=255)
                             tcgttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATc                                                                                                                        >  2:1184504/1‑151 (MQ=255)
                              cgttcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCg                                                                                                                       <  1:621904/151‑1 (MQ=255)
                                 tcgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGt                                                                                                                    >  1:612611/1‑151 (MQ=255)
                                  cgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGt                                                                                                                    <  1:1337014/150‑1 (MQ=255)
                                  cgCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGt                                                                                                                    <  2:516741/150‑1 (MQ=255)
                                   gCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTg                                                                                                                  >  2:143089/1‑151 (MQ=255)
                                   gCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTg                                                                                                                  >  2:350032/1‑151 (MQ=255)
                                    ccTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTg                                                                                                                  >  2:1099798/1‑150 (MQ=255)
                                    ccTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGc                                                                                                                 >  1:297399/1‑151 (MQ=255)
                                     cTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGct                                                                                                                >  2:1123840/1‑151 (MQ=255)
                                     cTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGct                                                                                                                >  2:802131/1‑151 (MQ=255)
                                       gacgacGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGctct                                                                                                              <  2:737936/151‑1 (MQ=255)
                                       gacgacGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGctct                                                                                                              <  1:1116472/151‑1 (MQ=255)
                                       gacgacGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGct                                                                                                                <  1:294143/149‑1 (MQ=255)
                                           acgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACt                                                                                                          >  2:164718/1‑151 (MQ=255)
                                           acgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACt                                                                                                          >  2:553395/1‑151 (MQ=255)
                                           acgCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACt                                                                                                          <  1:187174/151‑1 (MQ=255)
                                              cagcaCCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACg                                                                                                       >  2:277675/1‑151 (MQ=255)
                                                 caCCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGa                                                                                                      >  2:399871/1‑149 (MQ=255)
                                                   ccGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGAc                                                                                                  <  1:20880/151‑1 (MQ=255)
                                                      cACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTc                                                                                               >  2:101273/1‑151 (MQ=255)
                                                         ggATATGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGc                                                                                            <  1:1229165/151‑1 (MQ=255)
                                                          gATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCa                                                                                           <  1:1220289/151‑1 (MQ=255)
                                                              cGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAAt                                                                                        <  1:734527/150‑1 (MQ=255)
                                                              cGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATc                                                                                       <  2:733161/151‑1 (MQ=255)
                                                                  ccAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGAc                                                                                    >  2:633267/1‑150 (MQ=255)
                                                                    agcagTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAg                                                                                 <  2:225019/151‑1 (MQ=255)
                                                                      cagTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGcc                                                                               >  1:1085081/1‑151 (MQ=255)
                                                                      cagTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGcc                                                                               >  1:1165975/1‑151 (MQ=255)
                                                                         ttCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCAt                                                                             <  1:806522/150‑1 (MQ=255)
                                                                          tCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCt                                                                           >  1:993837/1‑151 (MQ=255)
                                                                           cACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCt                                                                           <  1:618259/150‑1 (MQ=255)
                                                                           cACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCt                                                                           <  1:1216448/150‑1 (MQ=255)
                                                                             cgcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCtt                                                                          <  1:628918/149‑1 (MQ=255)
                                                                             cgcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCa                                                                        <  2:1241871/151‑1 (MQ=255)
                                                                              gcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCAc                                                                       <  1:968039/151‑1 (MQ=255)
                                                                              gcgGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCAc                                                                       >  2:1247653/1‑151 (MQ=255)
                                                                                gggTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCa                                                                     <  1:691954/151‑1 (MQ=255)
                                                                                gggTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCa                                                                     <  2:142261/151‑1 (MQ=255)
                                                                                 ggTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAg                                                                    <  1:167092/151‑1 (MQ=255)
                                                                                  gTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCGCTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAgg                                                                   <  2:823218/151‑1 (MQ=255)
                                                                                  gTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAgg                                                                   <  2:486286/151‑1 (MQ=255)
                                                                                   ttAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGt                                                                  >  2:390789/1‑151 (MQ=255)
                                                                                    tAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGt                                                                  >  2:621449/1‑150 (MQ=255)
                                                                                    tAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGt                                                                  >  2:215561/1‑150 (MQ=255)
                                                                                     aaaCGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTTCGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTa                                                                 <  2:632385/150‑1 (MQ=255)
                                                                                     aaaCGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTa                                                                 <  2:1100677/150‑1 (MQ=255)
                                                                                      aaCGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAAc                                                               <  1:150392/151‑1 (MQ=255)
                                                                                         ggTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACAtt                                                            <  2:259911/151‑1 (MQ=255)
                                                                                               ggAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATcc                                                      <  2:1298243/151‑1 (MQ=255)
                                                                                               ggAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATcc                                                      <  1:113042/151‑1 (MQ=255)
                                                                                                gAATGTAGTCGTCAGCGCCAATCTCCGGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCa                                                     <  2:777083/151‑1 (MQ=255)
                                                                                                   tGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGt                                                   >  2:325462/1‑150 (MQ=255)
                                                                                                     tAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTAcc                                                >  2:557335/1‑151 (MQ=255)
                                                                                                        tcgtcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCAt                                              >  1:1195105/1‑150 (MQ=255)
                                                                                                        tcgtcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCAt                                              >  2:30922/1‑150 (MQ=255)
                                                                                                           tcAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATa                                             >  1:379518/1‑148 (MQ=255)
                                                                                                             aGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAGGCCGTCGCCAAATCGCCAAGCCTTCTTCACCAGGAAAAATTAAATCCAGAACAATAAGATg                                        >  1:700497/1‑151 (MQ=255)
                                                                                                             aGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATg                                        >  1:1238173/1‑151 (MQ=255)
                                                                                                             aGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATg                                        <  2:567539/151‑1 (MQ=255)
                                                                                                                 ccAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGaaa                                    <  2:848554/151‑1 (MQ=255)
                                                                                                                 ccAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGaaa                                    <  1:802121/151‑1 (MQ=255)
                                                                                                                   aaTCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGa                                  <  1:773009/151‑1 (MQ=255)
                                                                                                                        ccAGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTc                               <  2:468044/149‑1 (MQ=255)
                                                                                                                        ccAGGCCTACGATACGGTCCACTTCTTCCCCTTCTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGAtt                                <  1:1217183/148‑1 (MQ=255)
                                                                                                                          aGGCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACg                            >  2:63171/1‑150 (MQ=255)
                                                                                                                           ggCCTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAg                          <  1:421663/151‑1 (MQ=255)
                                                                                                                             ccTACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTc                        >  1:838913/1‑151 (MQ=255)
                                                                                                                               tACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTc                        >  2:1102927/1‑149 (MQ=255)
                                                                                                                               tACGATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTc                        >  2:1107953/1‑149 (MQ=255)
                                                                                                                                  gATACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTcagcag                   <  1:153365/151‑1 (MQ=255)
                                                                                                                                   aTACGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAgg                  >  1:801705/1‑151 (MQ=255)
                                                                                                                                     aCGGTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGc                 <  1:320519/150‑1 (MQ=255)
                                                                                                                                        gTCCACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATc             >  2:1287247/1‑151 (MQ=255)
                                                                                                                                           cACTTCTTCCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCAt          <  2:690885/151‑1 (MQ=255)
                                                                                                                                               tcttcCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGt      >  2:495551/1‑151 (MQ=255)
                                                                                                                                                cttcCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGtt     >  2:1082102/1‑151 (MQ=255)
                                                                                                                                                cttcCCCTTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGtt     >  2:935980/1‑151 (MQ=255)
                                                                                                                                                 tcccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTc    <  2:1080454/148‑1 (MQ=255)
                                                                                                                                                 tcccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTc    <  1:704264/148‑1 (MQ=255)
                                                                                                                                                  cccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCt   <  1:1276807/149‑1 (MQ=255)
                                                                                                                                                  cccctTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATCAAATCCAGTGCCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTg  <  1:274538/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CCTCTTCCTGTGACGGTGCGCCTGGCAGTTCGTTCGCCTGACGACGCAGCACCGCACGGATACGGGCCAGCAGTTCACGCGGGTTAAACGGTTTTGGAATGTAGTCGTCAGCGCCAATCTCCAGGCCTACGATACGGTCCACTTCTTCCCCTTTCGCCGTCACCATAATGATCGGCATCGGGTTGCTCTGACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTG  >  NC_000913/3536179‑3536475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: