Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA48.3% 60.2 / 39.7 60intergenic (‑124/+79)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (30/1);  new base A (0/29);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 5.24e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT  >  NC_000913/1824911‑1825211
                                                                                                                                                        |                                                                                                                                                      
cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                                        <  1:870911/151‑1 (MQ=255)
    ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                                     >  1:28145/1‑150 (MQ=255)
     tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAgaaaagaaaa                                                                                                                                                    <  2:839684/150‑1 (MQ=255)
     tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAc                                                                                                                                                   >  1:400736/1‑151 (MQ=255)
       cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGACCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                                 <  1:1245857/151‑1 (MQ=255)
        gCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgc                                                                                                                                                >  2:481637/1‑151 (MQ=255)
          atatCAGAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgccg                                                                                                                                              >  2:1193116/1‑151 (MQ=255)
          atatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgccg                                                                                                                                              >  1:145135/1‑151 (MQ=255)
                aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                        >  1:197919/1‑151 (MQ=255)
                    aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                                     >  2:629751/1‑150 (MQ=255)
                      tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                                  >  2:128902/1‑151 (MQ=255)
                       ccATCCCCGCCCCCGGCCCTTTTTTTTTTTTTTTATCGTTCAGTTACCCGTGGATGTTTATGGGCAACTGGGTTGTTTTACGTTAGTGGTAATCAGTTTTTTTTTACAACTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCg                                                                                                                                 <  2:921407/151‑1 (MQ=255)
                        cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc                                                                                                                                   >  2:230553/1‑148 (MQ=255)
                        cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                                >  2:260838/1‑151 (MQ=255)
                           aCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                             >  1:1280824/1‑151 (MQ=255)
                             tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                           >  1:915482/1‑151 (MQ=255)
                                  cccGGCCTTTTTTTTTCATTTTTTTCTTTAAGGTAACCGGGGATGTTTACCGGAAACTGGGGTGTTTTACGTTAGTGGTGATCAGCTTTTTTTTAAAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                        <  1:1088506/149‑1 (MQ=255)
                                  cccGCCCCGTTTTTTTCTTTTTTATCGTTCAGGTAACGGGGGATGGTTATCGGCAACTGGGGTGATTTACGTTGGTGGTGATCGGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa                                                                                                                      <  2:211573/151‑1 (MQ=255)
                                     ggCCCTTTCTTTTCATTTTTTTCTTTCAGGTACCGGTGGATGGTTATCGGCACCTGGGGTGATTTACTTTAGTGGTGATCGGTTTTTTTTTACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                                    <  2:140780/150‑1 (MQ=255)
                                     ggCCCGTTTTTTTCATTTTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                                    <  2:217938/150‑1 (MQ=255)
                                         cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                               >  1:734545/1‑151 (MQ=255)
                                                ttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                           >  1:328487/1‑148 (MQ=255)
                                                   aTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                                     >  1:888547/1‑151 (MQ=255)
                                                    tttttATCGTTGAGGAAACCGTGGATGTTTTTCGACAACTGGGGTGATTTGCGTTAGTGGTGATAAGGTTTTTTTAACAATGGTTTAAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                      <  2:202235/146‑1 (MQ=255)
                                                    ttGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                                    >  1:1033478/1‑151 (MQ=255)
                                                     ttttttCGTTCAGGTACCGGTGGAGGGTTTTCGGCAACTCGGGTTTTTTCCTTTAGTGGTGATCGGTTTTTTTTTACAATTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                                    <  1:429224/145‑1 (MQ=255)
                                                        tATCGTTCAGGTACCGGGGGTTGTTTACCGGCATTGCGGGTATTTTACTTTGGGGGTCACAAGGTTTTTTTCACAGTTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                                 <  1:412252/150‑1 (MQ=255)
                                                               cAGGTACCCGTGGATGTTTATCGGCAACGGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACATCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                          <  1:169234/150‑1 (MQ=255)
                                                                 ggTACCCGTGGATGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGAGGTCTTAAGt                                                                                         <  2:471810/149‑1 (MQ=255)
                                                                  gTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                                      >  2:801852/1‑151 (MQ=255)
                                                                   tAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt                                                                                     >  2:41517/1‑151 (MQ=255)
                                                                    aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGgttttt                                                                                    >  1:264751/1‑151 (MQ=255)
                                                                      cggTGGATGTTTACCGCCACCTGGGTTATTTTATTTTAGTGGTGATCGGTTTTTTTTTACAATTTTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTg                                                                                   <  2:666782/148‑1 (MQ=255)
                                                                          ggttgtttATCGGAACCTGGGGTAATTTACGTTAGTGGTGATCGGTTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                                 <  1:847930/142‑1 (MQ=255)
                                                                               tttAGCGGCAACTGGGGTGATTTTCGTTATTGGGAACGCGTTTTTTTTTCCAGCTTTTTGAGAGAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg                                                                           <  1:1191164/148‑1 (MQ=255)
                                                                                ttATCGGCACCTGGGGTTATTTATTTGGGTGGTGATCAGTTTTTTTTTAAAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                               <  1:345133/144‑1 (MQ=255)
                                                                                     ggcatcgggggTGTTTTACGTGGGGGTTGACAGGCTTTTTTTAAGACTGATTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                                            <  2:967205/134‑1 (MQ=255)
                                                                                      gCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGattttt                                                                     >  1:221468/1‑148 (MQ=255)
                                                                                      gCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                                     >  2:373307/1‑148 (MQ=255)
                                                                                        accgggggttttttACGTTAGGGGTGATCGGTTTTTTTTTAAAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                                   <  1:409656/137‑1 (MQ=255)
                                                                                             gggTGATTTACGTTAGTGGTGATCAGGTTTTTTTTAAAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                             <  1:228142/149‑1 (MQ=255)
                                                                                              ggtttttttagTTAGTGGTGATCGGTTTTTTTTTACATTTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                           <  1:387703/140‑1 (MQ=255)
                                                                                                tGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGGTTTTTGGTTGAAAGATTATTTAGCAGGTGCAgg                                                        >  1:557097/1‑151 (MQ=255)
                                                                                                   tttACTTTAGTGGTGATCAGTTTTTTTTTACAATTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                                                         <  1:449642/147‑1 (MQ=255)
                                                                                                    ttACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCGGTTGCAGGCAtt                                                    >  2:1265017/1‑151 (MQ=255)
                                                                                                         ttggtggtcATCGGTTTTTTTTTACATTTTTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                                <  2:696820/147‑1 (MQ=255)
                                                                                                              ggtgaccggtttttttttACAACTGTTTAAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                          <  2:755306/140‑1 (MQ=255)
                                                                                                              ggtgATCGGTTTTTTTTAAAACCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                              <  1:1085342/147‑1 (MQ=255)
                                                                                                                     agtttttttttACAATTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                                   <  2:754713/147‑1 (MQ=255)
                                                                                                                        cTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg                                >  2:284813/1‑151 (MQ=255)
                                                                                                                             tttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                            >  2:709470/1‑150 (MQ=255)
                                                                                                                                acacCTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                         <  1:915533/150‑1 (MQ=255)
                                                                                                                                aCAATTGTTTGAAAAAAAAAAGAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                        <  1:285860/151‑1 (MQ=255)
                                                                                                                                aCAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                         >  2:1231291/1‑150 (MQ=255)
                                                                                                                                aCAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                         >  2:3893/1‑150 (MQ=255)
                                                                                                                                  aaCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                        <  1:951727/149‑1 (MQ=255)
                                                                                                                                  aaCTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGGCGTTCTGTc                         >  1:307409/1‑148 (MQ=255)
                                                                                                                                  aaCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                        <  1:709974/149‑1 (MQ=255)
                                                                                                                                     cTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                     >  1:104107/1‑150 (MQ=255)
                                                                                                                                         tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt                 >  2:1302442/1‑151 (MQ=255)
                                                                                                                                          tggaaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                          <  2:40586/136‑1 (MQ=255)
                                                                                                                                             agaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt                 <  1:336591/147‑1 (MQ=255)
                                                                                                                                               aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGATTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCCCTGGACGGTCTGTCAGACGCCtctc              >  1:604642/1‑148 (MQ=255)
                                                                                                                                                  aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaaa          <  1:1325005/147‑1 (MQ=255)
                                                                                                                                                  aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAt         <  1:129731/148‑1 (MQ=255)
                                                                                                                                                   gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt        >  1:1178142/1‑150 (MQ=255)
                                                                                                                                                    aaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGTACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAg      <  2:357438/151‑1 (MQ=255)
                                                                                                                                                    aaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTa       <  2:959878/150‑1 (MQ=255)
                                                                                                                                                     aaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc     <  2:459997/151‑1 (MQ=255)
                                                                                                                                                        gaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGGTTATTCAGCCGTTGCAGGCCTTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAtt  >  2:1191207/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
CATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT  >  NC_000913/1824911‑1825211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: