Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,660	0	G	T	47.5%	84.4 / 37.9	61	E321* (GAA→TAA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base G (4/28);  new base T (29/0);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 2.55e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCT  >  NC_000913/2252511‑2252810                                                                                                                                                       |                                                                                                                                                         aGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGa                                                                                                                                                         <  2:513710/151‑1 (MQ=255)        gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGAt                                                                                                                                                    <  2:1139647/149‑1 (MQ=255)            gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGtt                                                                                                                                              <  2:1261593/151‑1 (MQ=255)                aTTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATAT‑TGCTTTTTTTTAGGGTACAGgtt                                                                                                                                             >  2:302134/1‑148 (MQ=255)                 tttGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATAT‑AACTTTTTTTTAGGATAAAGtttt                                                                                                                                            >  1:800446/1‑147 (MQ=255)                  ttGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTAT‑AAGTTTTTTTTAGGAGAAAGttttc                                                                                                                                           >  1:496510/1‑146 (MQ=255)                     cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACa                                                                                                                                     <  2:34438/151‑1 (MQ=255)                     cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTT‑TGCTTTTTTTTAGGAGAAAGTTTTcgc                                                                                                                                         >  1:6783/1‑147 (MQ=255)                        gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTTGGGTAT‑AAGTTTTTTTCAAGAAACAGttt                                                                                                                                             >  2:298432/1‑140 (MQ=255)                        gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAgcg                                                                                                                                  <  1:985533/151‑1 (MQ=255)                          tGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTAT‑AGATTTTTTTTAGGATACAGATTTTGCGACAGCGcc                                                                                                                                >  1:963357/1‑151 (MQ=255)                           gAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGccc                                                                                                                               <  1:498983/151‑1 (MQ=255)                           gAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGccc                                                                                                                               <  1:483621/151‑1 (MQ=255)                                aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTGTAT‑AAATTTCTTTTAAGGTAAAGTTTTCGGGACAACcccccgttt                                                                                                                          >  1:1174426/1‑146 (MQ=255)                                  tttATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTT‑AACTTTTTTTTAGGAAACAGATTCCCCGAGCGCGCCCGctt                                                                                                                           >  1:394857/1‑148 (MQ=255)                                   ttATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATAT‑AGCTTTTTTTTAGGGTAAAATTTTCCCCGCAGCGCCCGgttt                                                                                                                          >  2:202277/1‑148 (MQ=255)                                           gCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTGTAT‑AGCTTTTTTTTAGGATACAGTTTTCGCGCCAGCGCCCCGTTTTCGGTTGCCt                                                                                                                >  1:758110/1‑150 (MQ=255)                                             gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTTT‑AGGTTTCTTTATGGATAAAGTTTTCGCGGCAACGCCCGGTTTGCGGTGGcccg                                                                                                               >  1:661560/1‑147 (MQ=255)                                             gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTAT‑AGCTTTTTTTTAGGATAAAGTTTTCCCGACAGCGccccctttt                                                                                                                         >  2:443760/1‑133 (MQ=255)                                             gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAAGTTTTTTTTAAGGAAAAAGTTT‑CGCGGCAGCGCCCCGTTTTCggggggc                                                                                                                 >  2:585039/1‑142 (MQ=255)                                                 aGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑c                                                                                                        <  1:1016712/151‑1 (MQ=255)                                                  ggATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑Ca                                                                                                       <  1:526263/151‑1 (MQ=255)                                                    aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATAT‑AGCTTTTTTTTAGGGAAAAGTTTTCCCGGCAGCGCCCGCTTTTCGGTAGCCTGCGCgg                                                                                                          >  1:1063226/1‑147 (MQ=255)                                                       gagaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATCT‑AGCCTTCTTTGAGGATACACTTTTTTCGGCGGCGCCCGCTTTTCGGTGGTCCGCGCGG‑cgacttt                                                                                                  >  1:1339003/1‑145 (MQ=255)                                                            cAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTATAT‑AGCTTTCTTTAAGGGTACAGTTTTCTCGACAGCGCCCGGTTTTCGGTGGCCTGCGCGG‑CAAttt                                                                                                   >  2:871400/1‑145 (MQ=255)                                                            cAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTGTAT‑AAATTTTTTGTAGGGTACAGGTTTCGCGGAAGCGCCCGGTTTTCGGTAGGCGGCGCGG‑GGATTTtgcgc                                                                                              >  1:1188678/1‑150 (MQ=255)                                                              aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATAT‑AGCTTTTTTTTAGGGTAAAGTTTTCGCCCCAGCGCCCCTTTTGCGGGGGCCTCCGCCG‑GCATTTTTCGCGcc                                                                                           >  2:269934/1‑151 (MQ=255)                                                                 tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAg                                                                                         <  1:966913/150‑1 (MQ=255)                                                                    gCTGACCGCGCCAGCGCATACGTCAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATAT‑AACTTTTTTGTAGGAGAAAATTTTCGCGACAGCGCCCGCTTTTCGGTAGCCCGCGCGG‑GAAATTTTCGCGCCCGCgg                                                                                      >  2:108986/1‑150 (MQ=255)                                                                     cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATAT‑AGCTTTTTTTTAGGATACAGTTTTCGCGGCAGCGCCCGTTTTTCGGGAGCCTGCGCGGCCAATTTCGC‑CGCCCGCgg                                                                                      >  1:140455/1‑149 (MQ=255)                                                                      tGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGACTGGTGAGT‑GTGTTTCTTTGAAGATTTACTTTGCGCCCGACCACCCGTTTTGCGGTGGCCTTCGCCG‑GAATTTct                                                                                                 >  2:131100/1‑136 (MQ=255)                                                                        aCCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATAT‑AACTTTTTTTAAGGATAAAGCTTCCGCGACAGCGCCCGTTTTTCGGGAGCCTGCGCGG‑CAAATTTGCGCGCCCGCGGCAg                                                                                   >  2:517137/1‑149 (MQ=255)                                                                         ccGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑Ctt                                                                               <  1:585182/151‑1 (MQ=255)                                                                           gcgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGGTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTa                                                                              <  2:514793/150‑1 (MQ=255)                                                                           gcgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTAT‑AGCTTTTTTTAAGGATACAATTTTCCCGACAACGCCCCCTTTTTTGTAGCCCGCGCGG‑CAATTTCGTGCGCCAGCGGCGG‑CTTa                                                                              >  2:1335857/1‑150 (MQ=255)                                                                            cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCAGCAATAT‑AGATCTTTTTCTGGACACAGATTTCGCGGCAGAGCCCGCTTTTCGGGAACCCGTGCGG‑CCCATTCCCGCGCCAACGGCAG‑CCTaa                                                                             >  2:654793/1‑150 (MQ=255)                                                                                aGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAaacaac                                                                        <  1:1291223/151‑1 (MQ=255)                                                                                aGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATAT‑AGCTTTTTTTTAGGATAAAGGTTTCGCGCCAGCGCCCGCTTTTCGGTTGGCTGCGCGG‑CGATTTCTCGCGCCAGCGGCAG‑CCTaaaaaa                                                                         >  1:732731/1‑150 (MQ=255)                                                                                    cATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAacccta                                                                    <  2:1292060/151‑1 (MQ=255)                                                                                    cATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAacccta                                                                    <  1:810086/151‑1 (MQ=255)                                                                                    cATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATAT‑AACTTTCTTTAAGGGTACAGTTTTCGCCGCAGCGCCCCTTTTTCGGTAGCCTGCGCGG‑CAATTTTGCGCGCCCGCGGCCG‑CTTAAAAAaccc                                                                      >  2:1340554/1‑149 (MQ=255)                                                                                      tACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATAT‑AGCTTTTTTTTAGGAAAAAGGTTTCTCGACAGCGCCCGCTTTGCGGGAGGCTGCGCGG‑GAAATTTGCGCGCCCGCGGCCG‑CGTAAAAAacactacc                                                                  >  1:858817/1‑151 (MQ=255)                                                                                       aCGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAaccctaccc                                                                 <  2:813183/151‑1 (MQ=255)                                                                                        cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAaccctaccct                                                                <  2:611673/151‑1 (MQ=255)                                                                                         gACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATAT‑AACTTTTTTTTAGGGTACAGTTTTCGCGGCAGCGCCCCCTTTGCGGGAGCCTGCGCGG‑CAAATTTGCGCGCCCGCGGCCG‑CTTTAAAAaacccaccc                                                                 >  2:153283/1‑149 (MQ=255)                                                                                            aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCt                                                            <  2:416375/151‑1 (MQ=255)                                                                                                gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCg                                                        <  1:878730/151‑1 (MQ=255)                                                                                                     ttttGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATAT‑AGCTTTTTTTTAGGATACAATTTTCGCGGCAGCGCCCGCTTTTTGGGAGGCTGCGCGG‑CAATTTTTCGCGCCCGCGGCAGCCTTAAAAAACCC‑CCCCCGTCTGcccctaa                                                    >  1:1323585/1‑145 (MQ=255)                                                                                                             cTGACGATGGTTTTATGGCGGGCCTGGGATTT‑AACTTTTTTTTTGGGGAAAGTTTTCGCGACCGCGCCCCCTTTTCGGTAGCCTGCGCGG‑CAATTTTCCGCGCCCGCGGCCGCCTTAAAAAACCC‑ACCCCCTCTTCCCGTAACGCGCctt                                            >  1:1227825/1‑150 (MQ=255)                                                                                                               gACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                                         <  1:194795/151‑1 (MQ=255)                                                                                                                           aTGGCGGGCCTGGTATAT‑AGCTTTCTTTAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGGAGCCTGCGCGG‑CAATTTTGCGCGCCAGCGGCCG‑CTTAAACAACCCCACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg                             >  1:972469/1‑151 (MQ=255)                                                                                                                               cGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATggggga                         <  2:1293139/151‑5 (MQ=255)                                                                                                                                  gCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACa                      <  1:590109/151‑1 (MQ=255)                                                                                                                                      ggTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAt                    <  2:79419/149‑1 (MQ=255)                                                                                                                                         atat‑aGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg                <  1:1117618/150‑1 (MQ=255)                                                                                                                                         atat‑aGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg                <  1:1176597/150‑1 (MQ=255)                                                                                                                                            t‑aGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCgg            >  2:257318/1‑151 (MQ=255)                                                                                                                                               gCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGAt          <  2:694276/151‑1 (MQ=255)                                                                                                                                                  ttCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCg       >  1:6476/1‑151 (MQ=255)                                                                                                                                                      tGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGa      <  1:1157362/148‑1 (MQ=255)                                                                                                                                                      tGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGAc     <  2:661569/149‑1 (MQ=255)                                                                                                                                                       gAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGc   <  1:443754/150‑1 (MQ=255)                                                                                                                                                       gAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCt  <  2:1126077/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                         AGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATAT‑AGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGG‑CAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCT  >  NC_000913/2252511‑2252810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑