Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,748	0	C	A	46.2%	78.7 / 65.4	91	E55* (GAG→TAG)	nudF	ADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (38/11);  new base A (0/42);  total (38/53)
Fisher's exact test for biased strand distribution p-value = 5.85e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTT  >  NC_000913/3177601‑3177895                                                                                                                                                    |                                                                                                                                                    tttCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCaa                                                                                                                                                   >  2:632943/1‑150 (MQ=255)  ttCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCaaa                                                                                                                                                  <  2:121666/150‑1 (MQ=255)     aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                              >  1:473329/1‑151 (MQ=255)     aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                              >  1:285006/1‑151 (MQ=255)     aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                              >  2:672808/1‑151 (MQ=255)           ttCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCCATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTCACCGCGCTCAAAAATTTccc                                                                                                                                          >  2:226891/1‑149 (MQ=255)            tCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                                                                                                       >  2:334678/1‑151 (MQ=255)             cAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                                                                                                       >  2:874322/1‑150 (MQ=255)                   tcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTc                                                                                                                                >  2:664215/1‑151 (MQ=255)                                ccccAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCAttt                                                                                                                     <  1:494788/147‑1 (MQ=255)                                 tcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGcc                                                                                                                  >  1:1328888/1‑151 (MQ=255)                                  ccAGTAGCCAGGGGGTTTCGCTGGTGTCGTCCGCGGCAATCCGAATCTGCTCAACCAGCACAACTTCATCACGCACGGGGTCAAAGGGTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGccc                                                                                                                 <  1:154004/151‑1 (MQ=255)                                  ccAGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAGGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGcc                                                                                                                  <  2:220871/150‑1 (MQ=255)                                  ccAGTAGCCAGGGGGTTCCGCTGGTGTCGTACGCGGAAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACGGGGCAAAGGGGTAGCAAATCTGCGGCTTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGccc                                                                                                                 <  1:1237870/151‑1 (MQ=255)                                      tAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                                                                                             >  2:355755/1‑151 (MQ=255)                                       aGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                                                                                             >  2:397555/1‑150 (MQ=255)                                         ccAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                          >  2:834066/1‑151 (MQ=255)                                           aggggtTTTCGCTGGTGTCGTGCGCGGCAACCCGAATCTGCTCAACCAGCACAACTCCACCACGCACTGGGTAAAAGGGTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAg                                                                                                        <  1:810785/149‑1 (MQ=255)                                           agggggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCCCGCACTGGGTCAAAGGGTAGCAAGACGGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAg                                                                                                        <  1:1316068/149‑1 (MQ=255)                                           agggggTTTCGCGGGTGTCGTCCGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCCCGCACGGGGTCAAAGGTTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                         <  2:597055/148‑1 (MQ=255)                                           aaGGGGTTTCGCTGGTGTCGTCCGCGGCAATCCGAATCTGCTCAACCAGCACAACTTCACCCCGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                         <  1:888839/150‑1 (MQ=255)                                           aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                          >  2:53183/1‑149 (MQ=255)                                             ggggTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGa                                                                                                       <  1:500187/150‑1 (MQ=255)                                                   tCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                                                                                                >  2:1019542/1‑151 (MQ=255)                                                      cTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGa                                                                                              >  1:379770/1‑150 (MQ=255)                                                      cTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGa                                                                                              >  2:589516/1‑150 (MQ=255)                                                       tGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaa                                                                                             >  1:710456/1‑150 (MQ=255)                                                        ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaa                                                                                             <  2:429312/149‑1 (MQ=255)                                                         gtgtCGTACGCGGCAATCCGAATCTGCTAAACCACCAAACCTTCATCACGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                          <  2:1202380/151‑1 (MQ=255)                                                            tCGTCCGCGGCAATCCGAATCTGCTCAATCACCACACCTTCACCCCGCACTGGGTCAAGGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                          <  2:36980/148‑1 (MQ=255)                                                             cGTCCGCGGCAATCCGAATCTGCTCAATCAGCACAACTCCACCACGCACTGGGTAAAGGGGTAGCAAGCCTGCGGCTTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt                                                                                         <  1:1155503/148‑1 (MQ=255)                                                             cGTACGCGGCAACCCGAATCTGCTCAACCAGCAAAACTTCATCCCCCCCTGGGTAAAGGGGTAGCAAGCCTGGGGCGTGACCGCGAAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                      <  1:386880/151‑1 (MQ=255)                                                              gTACGCGGCAATCCGAATCTGCTCAATCACCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                     <  1:993228/151‑1 (MQ=255)                                                                aCGCGGCAATCGGAATCTGCTAAACCACCAAAACTTCATCTCGCACGGGGTAAAAGGGTAGAAAGACTGCGGCGTGACGGCCCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                     <  2:848998/149‑1 (MQ=255)                                                                 cgcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaaa                                                                                    <  1:656950/149‑1 (MQ=255)                                                                 cgcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGa                                                                                  <  2:833221/151‑1 (MQ=255)                                                                  ggggCAATCCGAATCTGCCCAACCACCACAACTTCATCCCGCACGGGGTCAAAGGGTAGAAAGACTGCGGCGTGCCCGTGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaaa                                                                                    <  1:878692/146‑1 (MQ=255)                                                                  gcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAg                                                                                   <  2:243016/149‑1 (MQ=255)                                                                       aaTCCGAATCTGCACAATCAGCACAACTCCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCACAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              <  1:1028352/149‑1 (MQ=255)                                                                          ccGAATCTGCTCAATCACCACAACTCCACCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                         <  1:766997/151‑1 (MQ=255)                                                                             aatcctcttaaTGAGCAAAATTCCACCCCGCACTGGGTCAAAGGGTAGCAAGATCGCGTTGTGACCGCACAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaa                                                                      <  1:1250098/142‑1 (MQ=255)                                                                             aaTCTGCTCAATCAGCACAACTTCACCACGCACGGGGTCAAGGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaa                                                                        <  1:854772/149‑1 (MQ=255)                                                                              aTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaa                                                                      >  1:511109/1‑150 (MQ=255)                                                                              aTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaa                                                                      >  2:29252/1‑150 (MQ=255)                                                                              aTCTGCTAAATCAGCACAACTTCATCACGCATGGGGTAAAGGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaa                                                                        <  1:483760/148‑1 (MQ=255)                                                                               tCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaaa                                                                     >  2:1134218/1‑150 (MQ=255)                                                                                  gCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                                 >  1:1133603/1‑151 (MQ=255)                                                                                  gCTAAATCAGCACAACTGCATCACGCACTGGTTAAAAGGGAGGCAACACGGGGGCGTGACCTCGTTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                                 <  2:689690/151‑1 (MQ=255)                                                                                      aaTCAGCACATCTCCACCACACCCTGGGCAAAAGGATACCAAGGCTGCGCGTTCACCGCCATAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAg                                                                   <  1:350271/145‑1 (MQ=255)                                                                                      aaTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                              >  2:256333/1‑150 (MQ=255)                                                                                        tCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcacg                                                              >  1:1303243/1‑148 (MQ=255)                                                                                        tCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt                                                            >  2:1034399/1‑150 (MQ=255)                                                                                           caccaATTTCACCACCCGCTGGGTAAAGGGGTACGAACCCGGCGGCGTGACCGCCCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAg                                                        <  2:859682/148‑1 (MQ=255)                                                                                             acacCTTCATCACGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                       <  1:213629/150‑1 (MQ=255)                                                                                             acaACTCCATCACTCACTGGGTAAAAGGGTAGAAAGACTGCGGCGTGACCGCGCTCAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                       <  2:94795/150‑1 (MQ=255)                                                                                               atcTTCACCACGCACTGGGTCAAGGGGAAGCAAGACTGCGGGGGGACCGCTCAAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATaaa                                                         <  1:261924/144‑1 (MQ=255)                                                                                               aaCTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGtt                                                    >  2:1000680/1‑151 (MQ=255)                                                                                               aaCTCCACCACCCACGGGGACAAGGGGTAGAAAGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                       <  1:744276/148‑1 (MQ=255)                                                                                                aCTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGtt                                                    <  1:1161618/150‑1 (MQ=255)                                                                                                 cTTCACCCCGAACTGGGTAAAAGGGTAGCAAGTGTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGttt                                                   <  1:530283/150‑1 (MQ=255)                                                                                                  ttCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtct                                                 >  1:1334342/1‑151 (MQ=255)                                                                                                    catcaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtctc                                                <  2:823703/150‑1 (MQ=255)                                                                                                     atcaCGCACTGGGTCAAAGGGCAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCTCCGCACCTCATGACTCATTTTCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCg                                               >  2:797338/1‑150 (MQ=255)                                                                                                       caCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                            >  2:570431/1‑151 (MQ=255)                                                                                                       caCGCACTGGGTCAAAGGGTAGCAAGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg                                             <  2:719456/150‑1 (MQ=255)                                                                                                           cACTGGGTCAAGGGGTAGCAAGACTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         <  1:1120705/150‑1 (MQ=255)                                                                                                           cACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         >  2:280689/1‑150 (MQ=255)                                                                                                           cACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         >  2:764197/1‑150 (MQ=255)                                                                                                               gggTCAAAGGGTAGCAAGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAAttt                                    <  1:266309/151‑1 (MQ=255)                                                                                                               gggTAAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataat                                      <  1:947797/149‑1 (MQ=255)                                                                                                               gggTAAAAGGGAAGCAAGACGGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataa                                       <  1:291984/148‑1 (MQ=255)                                                                                                                    aaaGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTAc                                <  1:1268048/150‑1 (MQ=255)                                                                                                                     aaGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt                              >  2:212873/1‑151 (MQ=255)                                                                                                                      aGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                             >  2:1138756/1‑151 (MQ=255)                                                                                                                       gggcagaatcaCTGCGGCGTCACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCg                            <  2:806282/141‑1 (MQ=255)                                                                                                                       gggTAGCAGGACTGCGGCGTGACCGCGATAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                             <  1:705507/150‑1 (MQ=255)                                                                                                                       gggTAGCAAGACGGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                             <  2:670625/150‑1 (MQ=255)                                                                                                                        ggTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGt                           <  2:722964/151‑1 (MQ=255)                                                                                                                           aGCAAGCCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttt                         <  2:1341305/150‑1 (MQ=255)                                                                                                                             cAAGACGGCGGCGTGACCGCGCCAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt                       <  2:1190886/150‑1 (MQ=255)                                                                                                                              aaGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt                       <  1:1025560/149‑1 (MQ=255)                                                                                                                              aaGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGc                     >  2:336763/1‑151 (MQ=255)                                                                                                                              aaGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTg                      <  1:427948/150‑1 (MQ=255)                                                                                                                                    gcggcgTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATg               <  2:1292022/151‑1 (MQ=255)                                                                                                                                    gcggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAAt                >  2:1075381/1‑150 (MQ=255)                                                                                                                                     cggcgTGACCGCCCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATg               <  2:577891/150‑1 (MQ=255)                                                                                                                                      ggcgTGCCCGCCCTAAAAATTTGCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGt              <  2:196143/150‑1 (MQ=255)                                                                                                                                           gACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg        >  1:814338/1‑151 (MQ=255)                                                                                                                                            aCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc       >  2:1045236/1‑151 (MQ=255)                                                                                                                                             ccGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc       >  1:251594/1‑150 (MQ=255)                                                                                                                                                 gcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGtt  >  1:784819/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTT  >  NC_000913/3177601‑3177895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑