Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC39.7% 106.4 / 20.8 63T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (28/10);  new base C (0/25);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 9.93e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCAT  >  NC_000913/1376356‑1376648
                                                                                                                                                       |                                                                                                                                               
gCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAacac                                                                                                                                                >  2:910968/1‑151 (MQ=255)
  ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAacac                                                                                                                                                <  1:87592/149‑1 (MQ=255)
  ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAacac                                                                                                                                                <  1:128012/149‑1 (MQ=255)
   tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                              <  1:923374/150‑1 (MQ=255)
   tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                             >  1:288700/1‑151 (MQ=255)
    aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                             <  2:1083996/150‑1 (MQ=255)
    aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                             <  1:334151/150‑1 (MQ=255)
    aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                            <  1:543419/151‑1 (MQ=255)
       gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGcc                                                                                                                                         <  1:1032132/151‑1 (MQ=255)
       gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGcc                                                                                                                                         <  2:23799/151‑1 (MQ=255)
        ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCg                                                                                                                                        >  2:1353211/1‑151 (MQ=255)
          gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                      >  1:697569/1‑151 (MQ=255)
           aTGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATg                                                                                                                                     >  1:383655/1‑151 (MQ=255)
           aTGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATg                                                                                                                                     <  1:443296/151‑1 (MQ=255)
                cTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccgc                                                                                                                                >  2:380148/1‑151 (MQ=255)
                     cgcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGtt                                                                                                                            >  1:1030824/1‑150 (MQ=255)
                       cgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTAc                                                                                                                          >  1:872548/1‑150 (MQ=255)
                         tAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtc                                                                                                                       >  2:1133869/1‑151 (MQ=255)
                                   gggacccGAAAAAGGGGGGGTGTTCCCCGGCCCGTACGGAAACAGGTCGCCGCGCTTCCGGGAACCGTGTTTTTTCTCGACCTTTAAACCCGTTATAAGGATAAAATGATCACCCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                              <  2:707963/145‑1 (MQ=255)
                                    gcgcCCGAAAAATGGTGAGTCTTCCCCTGCGCGTCCGGGAAAAGGCCGCCGCGCCTGCGGGACCCGGGGTGTGTCCGGAACGTTTAAACCGCTATCGGGATAATATGATCACCCCCCTCGCTAATGCCCGCCGTTACATCGTCGAAAAc                                                                                                              <  1:1340320/149‑1 (MQ=255)
                                        ccGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                        >  1:27360/1‑151 (MQ=255)
                                        ccGAAAAATGGTGAGTGTTCCCCTGCGCGTACTGAAACGGGTCGCCGCGCGTCCCGGACCCGTGGTGTATCTCGACCTTTTAACCCGCTATCAGGATCATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                           <  1:949803/148‑1 (MQ=255)
                                           cAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                      >  2:198148/1‑150 (MQ=255)
                                            aaaaTGGTGGGTGTTCCCGGTCGCGTGGTGAACCAGGCCGCGGCGGGTCCGAGAACCGGGTTGTATCTCAAACAGTAAAACCCCTATCAGGAAATTATGAACAACCCCCGGGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                                     <  2:609163/150‑1 (MQ=255)
                                            aaaaTGGTGAGTATTCCCCTGCGCGTACGGGAACAGGTCGCCGCGCTTCCGGGACCCGTGGTGTATCTCAACCCTTTAACCCTCTATCAGAACCATATATTAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                        <  1:865660/147‑1 (MQ=255)
                                             aaaTGGGGAGTGTCCCCCTGCGCTTCCTGGAAAGGGCCGCCGCGCGTCCCGGAACGGTGGTTTATCTCAAACATTAAACCCCCTACCAGGATCATATGACCACCCCCCTCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACa                                                                                                   <  2:909089/151‑1 (MQ=255)
                                                gggTGAGTGGTTCAGGGCGTGTAGGGGAACAGGTCGCGGCGGTAACAGGACCGGGGGTGTCCCTGGAGCCATAACCCCGTTATCAGTAACATATCATCACCCCCCGCGCCATAGCCCGCCGTTACATCGTCGAAAACGa                                                                                                            <  2:352325/138‑1 (MQ=255)
                                                 ggTGAGTGTTTCCCTGCGGCTGTTGGAAGAGGCCGCCGCGGGTCCCGGACCCGGGGTTTTTCTAGAGCGGATAACCCGCAAACGGAATCATATGAACACCCCCCTCGCAGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                                 <  2:1301355/149‑1 (MQ=255)
                                                 ggTGAGTGATCCCCGGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGACCCTTTAACCCGCTATCAGGATAATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGt                                                                                                <  1:1330229/150‑1 (MQ=255)
                                                    gagtgtTTCCTGGCGCGTACGGGAACAGGCCCCCGCGCGTCCCGGAACCGGGGTGTATCTGGATCCTTTAAACCGCTATCAGGATAAAATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACa                                                                                             <  2:915300/150‑1 (MQ=255)
                                                        gttccccGGCGCGTGCTGGAAGGGGTCGCCGCGCGCACGGGACCCGTGGTTTATCTCGACCTGTTAACCCGATATCGGTATAATATGATAAACACCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                          <  2:787583/145‑1 (MQ=255)
                                                         aTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                       >  1:280516/1‑151 (MQ=255)
                                                            cccTGCGCGTCCGGGAACGGGTCGCCGCGCGTCCCGGAACGGTGGTGTATCTCGACCCTTTAACCCGCTATCAGGATAATATGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                        <  1:313931/147‑1 (MQ=255)
                                                            cccTGCGCGTACTGAAACAGGTCGCCGCGCGTCCCGGACCCGGGGTGTATCTCGACCCGTTAACCCGCTATCAGGATCATATGACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                      <  1:310453/149‑1 (MQ=255)
                                                            cccGGCGCGTTCTGGAAGGGGTCGCCGCGCTTCCGGGACCCGTGGTGTATCTAGAACCGTTAACCCTCTATAGGGTACAAATAACAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                      <  2:780383/149‑1 (MQ=255)
                                                             ccTGGGCGTACTGGACCAGTCCGCCGCGCTTACCGGACCCGGGGTTTATCACAACCCTTAAAACCGCTATAAGGAACAAATGATCACCCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                    <  2:629856/150‑1 (MQ=255)
                                                               tGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCg                                                                                  >  2:1189996/1‑150 (MQ=255)
                                                                   gcgTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAttt                                                                              >  2:273991/1‑151 (MQ=255)
                                                                        cgggacagggTCGCCGCGCGTACCGGACCGGTGGTGTATCCCGACCCTTTAACCCGTTTTCAGGATCATATGAACAACCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATc                                                                         <  1:64154/143‑1 (MQ=255)
                                                                        cTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATc                                                                         >  1:1233515/1‑151 (MQ=255)
                                                                          ggaaggggTCCCCGCGCGTACCGGACCGGTGGTTTATCGCGACCCTTTAACCCGCTATCAGAATAATATAACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTa                                                                           <  2:747174/141‑1 (MQ=255)
                                                                           gAACGGGCCCCCGGGGGTACGGAACCCGTGGTGTCTCGCGACCCTTAAACCCCCTATCAGCATAATATAAACAACCCCCTCCCAGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAttt                                                                              <  2:668115/143‑1 (MQ=255)
                                                                             aCAGGTCCCCGCGCGACCCGGACCCGTGGTTTATCTCGAACCGTTAACCCGCTATCAGGATCAAATGACCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                     <  2:280243/150‑1 (MQ=255)
                                                                               aGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                                   >  1:437641/1‑150 (MQ=255)
                                                                                  tCGCCCCTCTTACCGCACCTGGGGTGTATCTCGAACCTTTAACCCGCTATGTGGTTATTAGCATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                <  2:288994/150‑1 (MQ=255)
                                                                                    gccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                             >  2:1052033/1‑151 (MQ=255)
                                                                                    gccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                             >  2:279008/1‑151 (MQ=255)
                                                                                    gccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                             >  2:674820/1‑151 (MQ=255)
                                                                                       gcgcgTCCCGGACCCGTGGTTTATCTCGACCCGTAAACCCGCTATCAGGATAATATAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaa                                                           <  1:577135/150‑1 (MQ=255)
                                                                                           gTACCGGACCCGTGTTTTTTCTCGACCCGTTAACCCGCTTTCAGGATCAAATGAACACCCCCCTCGCCAATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                         <  1:293792/148‑1 (MQ=255)
                                                                                              ccGGAACGGTGGTTTTTCTCGACCCGTTAACCCGCTATCAGAATAATATGACCAACCCCCCCGCCGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAagaaga                                                      <  2:1240818/148‑1 (MQ=255)
                                                                                              ccGGAACCGTGGTGTATCTCGACCCGTAAACCCGCTATCAGGATAATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                                     <  1:486259/149‑1 (MQ=255)
                                                                                                    ccGTGGTGTATCCCGACCCTTTAACCCGCTATCAGAATCATATGATCACCCCCCGCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                <  2:1239315/148‑1 (MQ=255)
                                                                                                       tggtgTATAGCGACCCGTTAACCCGCTATCAGGATCATATGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgc                                             <  1:677931/148‑1 (MQ=255)
                                                                                                              tctcAAACCGTAAACCCGCTATCAGGATCATATAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                      <  2:527316/148‑1 (MQ=255)
                                                                                                               ctcGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                                    >  2:352129/1‑149 (MQ=255)
                                                                                                                   aaCCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                               >  1:988475/1‑150 (MQ=255)
                                                                                                                       gTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAc                          >  1:1198640/1‑151 (MQ=255)
                                                                                                                            aCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgcg                      >  2:309576/1‑150 (MQ=255)
                                                                                                                                         aTCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGt           >  2:943905/1‑149 (MQ=255)
                                                                                                                                         aTCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc          >  2:1104592/1‑150 (MQ=255)
                                                                                                                                            atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt      >  2:522284/1‑151 (MQ=255)
                                                                                                                                            atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt      >  1:424486/1‑151 (MQ=255)
                                                                                                                                               tGATCAACACACTCGCCGATGCGCGCCTTCACATCGTCGAAAACGATCTTAAACATCTACAGATTATAGGCGATTTCTATCACATCAATATCTACGAAGATAACCTGGCGCAGGCGCTGCATGACAAGCGCGACCTGCTCGGCCAt        >  2:817476/1‑146 (MQ=255)
                                                                                                                                                gATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat  >  1:1268026/1‑151 (MQ=255)
                                                                                                                                                    aaCACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc    <  1:1106494/145‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                               
GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCAT  >  NC_000913/1376356‑1376648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: