Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	39.4%	117.4 / 35.8	71	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (39/4);  new base A (0/28);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 7.79e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645                                                                                                                                                        |                                                                                                                                                       tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                        >  1:27385/1‑151 (MQ=255)   tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                       >  2:1295148/1‑150 (MQ=255)     aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                                    <  1:1303614/151‑1 (MQ=255)       gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                                   >  2:1156460/1‑150 (MQ=255)        tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                                 >  2:1044050/1‑151 (MQ=255)              ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGc                                                                                                                                           <  1:1254129/150‑1 (MQ=255)                    aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTa                                                                                                                                      >  1:1316434/1‑149 (MQ=255)                     tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGt                                                                                                                                   >  2:835396/1‑151 (MQ=255)                        ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCTCCATTAAtttt                                                                                                                                 >  2:1120102/1‑149 (MQ=255)                           ccACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACtt                                                                                                                             >  1:1235629/1‑151 (MQ=255)                               ttttttCTGCTCTTTTTTTTTTCATGCAATCTTCTTGCTGCGCAAGCTTTTCCA‑AAAACAGGTTAGATGATTTTTTTGTCGTTTAATCCCTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACtttt                                                                                                                           <  1:702337/149‑1 (MQ=255)                                tttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAg                                                                                                                         >  2:1273670/1‑150 (MQ=255)                                     cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                    >  1:439927/1‑150 (MQ=255)                                       gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                                  >  2:202915/1‑150 (MQ=255)                                           tttttttttCCATGCATTCTTCTTGCTGCGAAAGATTTTTCC‑AAAACAGTTAAGATTATTTTTTTGTCCTTTATTCCTTAAAAAACATAAAGCGCCCAAAAAAAAATATAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                    <  2:1358773/143‑1 (MQ=255)                                           gTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                                >  2:235727/1‑148 (MQ=255)                                           gTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                             >  1:191920/1‑151 (MQ=255)                                                tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                         >  2:122928/1‑150 (MQ=255)                                                  tttCATACAATCTCCTTTCTGCGCAAGAATTTTCA‑ATAGCAGTTTATAATATTTTTTTATCGCTTAAGCCGTGTAAAACTTACATGAGACACAAAAAAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                         <  2:989269/148‑1 (MQ=255)                                                   ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                                     >  1:201862/1‑151 (MQ=255)                                                    tCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgcc                                                                                                    >  2:1001972/1‑151 (MQ=255)                                                     cATGCATTCTTTTTGCGGCAAAGGCGTTTTCC‑AAAAAAGTTTAAATGTTTTTTTTCTCGTTTAATGCTTAAAAAACTTGAATGACCCAAAAAATAATAAAAAAACT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                   <  1:441431/151‑1 (MQ=255)                                                     cATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                   >  1:488501/1‑151 (MQ=255)                                                      aTGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgc                                                                                                  >  2:895803/1‑151 (MQ=255)                                                              ttcttGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                          >  1:117126/1‑151 (MQ=255)                                                                   gctgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                      >  2:696130/1‑150 (MQ=255)                                                                     tgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                   >  1:512287/1‑151 (MQ=255)                                                                       cgaaaGCTTTTCCA‑AAAACAGGTTAGATGATTTTTTTGTCGTTTAATCCTTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                      <  1:1345042/143‑1 (MQ=255)                                                                        gcAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                >  1:374216/1‑151 (MQ=255)                                                                        cgcaGCCTTTTCCAAGAACGGGTTAGATTTTTTTTTTGCGGTTAAAGGCATAAAAAACATGCAGAAGCAAAAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                                  <  1:1089957/147‑1 (MQ=255)                                                                          aaGCTTTTCCC‑AAAAAAGTTTAGATGATTTTTTTGTCTTTTAATGCCTGTAAAACATGCATGACCCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                                 <  1:1185519/148‑1 (MQ=255)                                                                               ttttCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                           >  2:132190/1‑149 (MQ=255)                                                                                   cc‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCATTTGCCAGAAATTCGTCGGTAAGc                                                                        >  1:1281838/1‑148 (MQ=255)                                                                                    caaGAACAGTTTAGATTATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                       <  2:1231362/147‑1 (MQ=255)                                                                                     aaaaactgttaaGATAATTTTTTTGTCGCTTAATGCCTGAAAACCTTTCATGACCAAAAAAAAAAAAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAt                                                                    <  1:912665/140‑1 (MQ=255)                                                                                        aacggtttAGATGATTTTTTTGTCGTTTAATGCCTAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                                 <  1:778002/145‑1 (MQ=255)                                                                                        aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCCGAATGCCGCCGTTTGCCTGAATTTCGTCGGTAAGCAGAttt                                                                  >  2:1082456/1‑150 (MQ=255)                                                                                        aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                  >  1:1184940/1‑150 (MQ=255)                                                                                        aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                                 >  1:510618/1‑151 (MQ=255)                                                                                        aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                                 >  1:30565/1‑151 (MQ=255)                                                                                         aCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                                >  2:1095136/1‑151 (MQ=255)                                                                                           aGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              >  1:1098455/1‑151 (MQ=255)                                                                                            ggTTAGATGATTTTTTTGTCGCTTAATGCCTAAAAAACATGAATGAGCAAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              <  1:631252/150‑1 (MQ=255)                                                                                               tAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                          >  1:648218/1‑151 (MQ=255)                                                                                                agatattttttttGTCTCTTAATGCCTGAAAAACTTGCATGACCCAAAAAATAAAAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                                         <  1:774746/143‑1 (MQ=255)                                                                                                      tttttttGTCGTTTAATGCCTGAAAAACATGCATGGCCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                    <  1:1003151/148‑1 (MQ=255)                                                                                                        tttttGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                     <  1:504364/147‑1 (MQ=255)                                                                                                         ttttGTCGTTTAATCCTTGAAAAACATGCATGACCAAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                                 <  1:856473/150‑1 (MQ=255)                                                                                                           ttGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                              >  1:563571/1‑151 (MQ=255)                                                                                                               cttttAATCCCTAAAAAACATGCATGACCCAAAAAAAAATAAAAAAATC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                            <  1:1130369/146‑1 (MQ=255)                                                                                                                 cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                         >  2:69289/1‑150 (MQ=255)                                                                                                                  ttAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                       >  1:664399/1‑151 (MQ=255)                                                                                                                  taaaTCCCTATAAAACATGCATGCGCCAAAAAATAAAAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                         <  2:1350695/147‑1 (MQ=255)                                                                                                                        cttgaaaaaCATGCATGACCAAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                                  <  1:725949/145‑1 (MQ=255)                                                                                                                         ttaaaaaaCATGCATGAGCCAAAAAAAAAAAAAAAAACT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                   <  2:1235603/144‑1 (MQ=255)                                                                                                                             aaaaCATGCATGAGCCAAAAAATAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                              <  1:1062441/149‑1 (MQ=255)                                                                                                                               aaCATGCATGACCCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                          <  1:584931/151‑1 (MQ=255)                                                                                                                                 catgcatgAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                         >  2:1262913/1‑150 (MQ=255)                                                                                                                                 catgcatgAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                          >  1:265486/1‑149 (MQ=255)                                                                                                                                  atgcatgAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                         >  1:928758/1‑149 (MQ=255)                                                                                                                                     catgTGCCACAAAATAATATAAAAAAT‑CTCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     >  2:757501/1‑150 (MQ=255)                                                                                                                                     catgAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     >  1:1004964/1‑150 (MQ=255)                                                                                                                                       tgACCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                    <  1:1241086/149‑1 (MQ=255)                                                                                                                                           ccAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     <  1:835411/144‑1 (MQ=255)                                                                                                                                             acaAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat            <  2:811950/151‑1 (MQ=255)                                                                                                                                             aaaaaaTAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa               <  2:1049551/146‑1 (MQ=255)                                                                                                                                             aaaaaaTAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat            <  1:129097/149‑1 (MQ=255)                                                                                                                                              caAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt           >  1:1107273/1‑151 (MQ=255)                                                                                                                                              aaaaaaaaaaaaaaaaaTCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                  <  2:439919/144‑1 (MQ=255)                                                                                                                                               aaaaaaATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                  <  1:86409/143‑1 (MQ=255)                                                                                                                                                aaaTAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         <  1:718516/151‑1 (MQ=255)                                                                                                                                                 aataatATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         >  2:243705/1‑150 (MQ=255)                                                                                                                                                  aaaataAAAAAATC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAACATGCAAATAAAGTGAGTGAATATTctct       <  2:880844/149‑1 (MQ=255)                                                                                                                                                    aataAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg      <  1:491742/150‑1 (MQ=255)                                                                                                                                                       ataAAATAT‑ACCGCCATTAAGTTGCCTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAgg  <  1:1239114/151‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                       TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑