Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,261,0550AC43.2% 151.7 / 15.8 90V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (41/9);  new base C (0/38);  total (41/49)
Fisher's exact test for biased strand distribution p-value = 1.87e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTT  >  NC_000913/3260909‑3261204
                                                                                                                                                  |                                                                                                                                                     
aGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc                                                                                                                                                   <  2:138407/151‑1 (MQ=255)
  tGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgc                                                                                                                                                 >  1:37490/1‑151 (MQ=255)
    aaTGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgccg                                                                                                                                               >  2:324079/1‑151 (MQ=255)
     aTGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgccg                                                                                                                                               >  1:804524/1‑150 (MQ=255)
     aTGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGt                                                                                                                                              >  2:365230/1‑151 (MQ=255)
      tGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGtt                                                                                                                                             >  1:1202852/1‑151 (MQ=255)
         tatTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAt                                                                                                                                          >  2:303404/1‑151 (MQ=255)
          atTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAtt                                                                                                                                         >  1:803080/1‑151 (MQ=255)
           ttCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTg                                                                                                                                        >  1:755291/1‑151 (MQ=255)
             cAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGGGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCg                                                                                                                                      >  1:222110/1‑151 (MQ=255)
                   gcttatttccTGCCCCGCCGCCCACCCAATGAAGTGATCGGGCCTGTCCACCTCTTTGTCGTAATCCACCACGTCCTCCATCAGCGGTGCTGTTTTCGGCCGCACTTGAATCTCCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTag                                                                                                                                   <  1:1209588/139‑1 (MQ=255)
                   gcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                                >  1:884553/1‑151 (MQ=255)
                    cTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                                >  2:361480/1‑150 (MQ=255)
                           actgacccGCCAGCCAGTCCATGAAGTGCTGGAGTCTGCCCATCATGTTGTCGAAATCCAGCTCGTCGTCCATCGGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCTTCCTCCCCGCCGTTAATTGCGTaga                                                                                                                                  <  2:425131/135‑1 (MQ=255)
                                cccGCCAGCCGTTCAATAAAGTGATCGAGGCTTTCCACCTCTTTTTCGTAATCCACCCCGTCGTCCACCAGGGGTGCTGTTTTCGCCCCGACCAGAATTTTCAGCTCCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCg                                                                                                                      <  2:841410/147‑1 (MQ=255)
                                cccGCCAGCCAGTCCATGAAGTGATCGAGGCTTCCCACCTCTTTGTCGAAATCCAGCTCGTCGCCCATCAGCGGTGTTGTTTTCGGCCCGACCTAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCa                                                                                                                   <  2:478973/150‑1 (MQ=255)
                                  cGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAgg                                                                                                                 >  1:619867/1‑151 (MQ=255)
                                  cGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAgg                                                                                                                 >  1:1344871/1‑151 (MQ=255)
                                   gccagccaGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                                                                                >  2:1225495/1‑151 (MQ=255)
                                    ccggccatTCCATGAAGTGACGGGGCCTGTCCATCTCTTTGTCTTAATCCAGCCCGTCGTCCATCAGCGGTGCTTTTTTCGGCCCGCCCTGAATCTTCAGTTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                                                                                <  1:736429/147‑1 (MQ=255)
                                    ccagccagTCCATGAAGTGATCGAGGCTGTCCATCTCTTTGTCGTAATCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                                                                                <  1:222798/150‑1 (MQ=255)
                                     cagccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtt                                                                                                               >  2:92350/1‑150 (MQ=255)
                                       gccagTCCATGAAGTGATCGAGGCTGTCCCTCCCTTGTTCGTAACCCAGCGCGCCGTCCCTCAGCGGTGCGGTTTCCGGCCCGACCAAAATCTTCATCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgc                                                                                                            <  1:17442/151‑1 (MQ=255)
                                        ccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcg                                                                                                           <  1:816734/151‑1 (MQ=255)
                                        ccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcg                                                                                                           >  2:1205536/1‑151 (MQ=255)
                                        ccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcg                                                                                                           >  1:1248739/1‑151 (MQ=255)
                                           gccgaTGAAGTGACCGGGGCTGCCCATCTTTTTGTGGTAACCCAGCCGGTCTTCCTCCGGCGGTGCGGTTTTCGGCCCACCCTGAATTTTCATTTTTGCTTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                                                                                <  2:324235/139‑1 (MQ=255)
                                                    ttGAGCGAGGCTTTCCATCTTTTTTTCGAATTCCAGCACGTCGTCCAGCAGCGGTGCTTTTTTCGGCCCGCCCTGAATCTTCAGCTTCGCCTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa                                                                                                 <  2:399084/148‑1 (MQ=255)
                                                    gTGATCGGGGCTGCCCATCCTTTTGTCGTAACCCAGCACGTCGTCCATCGGCGGTGCTGTTTCCGCCCCGCCCTAAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                                                                               <  1:599036/151‑1 (MQ=255)
                                                     tGATCGAGGCTGCCCTTCTCTTTGTCGAAATCCAGCCCGTCGTCCAGCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                                                                               <  2:1094748/150‑1 (MQ=255)
                                                      gaccGAGGCTGTCCATCTCTTTGTCGTAACCCAGCCCGTCGCCCATCAGGGGGGTTGTTTTCGGCCCGACCTGAATCTTCAGCTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGa                                                                                              <  2:1336610/147‑1 (MQ=255)
                                                      gATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGa                                                                                              >  2:483810/1‑150 (MQ=255)
                                                       aTCGAGGCTGTCCATCACTTTTTCTTAATCCAGCACGTCGTCCATCAGCGGTGTTTTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaa                                                                                                <  1:1316023/147‑1 (MQ=255)
                                                         cGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACt                                                                                           >  1:650180/1‑150 (MQ=255)
                                                           aGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc                                                                                         >  2:1305770/1‑150 (MQ=255)
                                                            ggcttcccATCTCTTTGTCGTAACCCAGCCCGTCGTCCACCAGGGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                                                                                       <  1:245890/145‑1 (MQ=255)
                                                              cTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                                                                                     >  2:815400/1‑151 (MQ=255)
                                                               tGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTg                                                                                    >  1:873594/1‑151 (MQ=255)
                                                                gTCCATCACTTTGTGGTAACCCAGCAGGTCGTCAACCAGCGGTGCTTTTTTGGCCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTg                                                                                    <  2:1108068/150‑1 (MQ=255)
                                                                  ccATCACTTTTTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                                                                                     <  1:1319155/147‑1 (MQ=255)
                                                                  ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCt                                                                                  <  1:1207426/150‑1 (MQ=255)
                                                                      cccTTTGTCGTAACCCAGCACGTCGCCCTTCAGCGGTGCTTTTTGCGGCCCGACCTGAATCTTAAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTGGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                                                                             <  2:488042/149‑1 (MQ=255)
                                                                         tttGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc                                                                          >  2:77520/1‑151 (MQ=255)
                                                                         tttGTCGTAACCCAGCCCGTCGTCCACCGGCGGTGCTTTTTGCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                                                                             <  1:513376/148‑1 (MQ=255)
                                                                         tttGTCGTAACCCAGCACGTCTTCCACCAGGGGTCCTTTTTTCGGCCCGCCCTAAATCTTAGGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCt                                                                                  <  2:5048/143‑1 (MQ=255)
                                                                           tGTCTTAACCCAGCACGTCCTCCATCACCGGTTCTGTTTTCGCCCCGCCCTGAATCTTCACCTCCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCa                                                                         <  2:206932/150‑1 (MQ=255)
                                                                                     cAGCACGTCGCCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCACCTCCTCTCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTg                                                              <  1:472227/151‑1 (MQ=255)
                                                                                       gctcgtcgcCCATCGGCGGTGCTGTTTTCGCCCCGACCTGAATTTTCACCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGc                                                                <  1:574528/144‑1 (MQ=255)
                                                                                        cACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACg                                                           >  1:526341/1‑151 (MQ=255)
                                                                                           gtcgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgc                                                          >  2:868016/1‑149 (MQ=255)
                                                                                           gtcgtcCATCAGAGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgc                                                          >  1:235346/1‑149 (MQ=255)
                                                                                              gtcCATCAGCGGTGCTGTTTTCGGCCCGCCCTGATTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagca                                                      <  1:137447/150‑1 (MQ=255)
                                                                                              gtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc                                                       >  1:348015/1‑149 (MQ=255)
                                                                                                 cATCAGCGGAGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc                                                   >  2:936766/1‑150 (MQ=255)
                                                                                                  aTCAGCGGTGCTGTTTTCGCCCCGACCTGAATCTTCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCa                                                  <  1:720933/150‑1 (MQ=255)
                                                                                                  aTCAGCGGTGCTGTTTCCGGCCCGATTTGAATCTTCACCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCa                                                  <  1:1173474/150‑1 (MQ=255)
                                                                                                    cAGCGGAGCTGTTCTCGGCCCGACCTGAATTCTCAGCTTCTCGTCCACCACGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTCCATCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCACCCGGCAAt                                                >  2:72397/1‑150 (MQ=255)
                                                                                                      gCGGGGCTGTTTTCGGCCCGGCAGGAATCTTCACCTCCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATc                                               <  1:850611/149‑1 (MQ=255)
                                                                                                        ggTGTTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg                                            <  1:751315/150‑1 (MQ=255)
                                                                                                         gTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGt                                           >  1:411290/1‑150 (MQ=255)
                                                                                                             tGTTTTCGCCCCCCCCTGAATCTTCGCTTTCTCTCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtc                                       <  2:192738/150‑1 (MQ=255)
                                                                                                               ttttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc                                    >  2:1150228/1‑151 (MQ=255)
                                                                                                                ttccGGCCCGACCTAAATCTTCGTCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc                                    <  2:1028790/147‑1 (MQ=255)
                                                                                                                   cGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                 <  1:428191/150‑1 (MQ=255)
                                                                                                                      cccGCCCTGATTCTTCATTTTCGCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGtt                              <  2:37490/150‑1 (MQ=255)
                                                                                                                       ccGACGTAATTCTTCATCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTg                             <  2:149013/150‑1 (MQ=255)
                                                                                                                       ccGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTg                             >  2:1089941/1‑150 (MQ=255)
                                                                                                                        ccaCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGaa                           <  2:729701/149‑1 (MQ=255)
                                                                                                                        cGACCGGATTTTACAGTTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGaa                           <  2:633539/151‑1 (MQ=255)
                                                                                                                         gACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGa                            >  2:421009/1‑149 (MQ=255)
                                                                                                                           ccTGATTTTTCATCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                        <  2:268213/151‑1 (MQ=255)
                                                                                                                           ccTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                        >  2:1034183/1‑151 (MQ=255)
                                                                                                                              gAATCTTCAGCTCCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCa                       <  1:92645/149‑1 (MQ=255)
                                                                                                                               attCTTCAGCTCCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGt                     <  2:110452/148‑1 (MQ=255)
                                                                                                                                 tCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTa                    <  1:467913/149‑1 (MQ=255)
                                                                                                                                 tCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg                  >  2:25709/1‑151 (MQ=255)
                                                                                                                                  cTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGc                 >  1:28488/1‑151 (MQ=255)
                                                                                                                                   ttCTGCTTCCCCTCCGCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGt                         <  2:536000/142‑1 (MQ=255)
                                                                                                                                   ttCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGc                 <  2:474271/150‑1 (MQ=255)
                                                                                                                                     cAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCAt               <  2:588711/150‑1 (MQ=255)
                                                                                                                                      atctgcgcctCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCAt               <  1:213926/140‑1 (MQ=255)
                                                                                                                                      aGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCa             <  1:365237/151‑1 (MQ=255)
                                                                                                                                        cTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc              >  2:1135630/1‑148 (MQ=255)
                                                                                                                                         ttCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc              >  1:373466/1‑147 (MQ=255)
                                                                                                                                         ttCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTAc                   <  2:76632/142‑1 (MQ=255)
                                                                                                                                             cGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTc      <  1:514497/151‑1 (MQ=255)
                                                                                                                                             cGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTc      >  2:1231624/1‑151 (MQ=255)
                                                                                                                                              gcccACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGAt          <  1:857935/144‑1 (MQ=255)
                                                                                                                                              gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTc      >  1:1164874/1‑150 (MQ=255)
                                                                                                                                                 cACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAttt  >  2:1300537/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTT  >  NC_000913/3260909‑3261204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: