Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,350,853	0	A	C	39.2%	144.6 / 12.7	79	S725A (TCA→GCA)	arcB	aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base A (37/11);  new base C (0/31);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 5.38e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGT  >  NC_000913/3350704‑3351001                                                                                                                                                      |                                                                                                                                                     ttttGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAc                                                                                                                                                     >  2:456672/1‑151 (MQ=255)    tGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                  >  2:213246/1‑151 (MQ=255)     gCCACCCACGCTTTCAGCACTTCTACGTCGTGTCGCCTCTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAATGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                  <  2:439204/150‑1 (MQ=255)     gCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                  <  1:69431/150‑1 (MQ=255)      ccacccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgc                                                                                                                                                <  2:847322/151‑1 (MQ=255)        acccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgccg                                                                                                                                              >  1:477868/1‑151 (MQ=255)        acccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgccg                                                                                                                                              >  2:312610/1‑151 (MQ=255)                 ttCAGCCCTTCTCCGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATCCCCCGACGTTATCTCCCCGGGCCGGAAGGTCAGGAGACTGATTTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTa                                                                                                                                      <  1:1275652/150‑1 (MQ=255)                      cACTTCTACGTCGTGACCCCACTCTTTTTTCATCTCTACAACCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAAATTTGCGGCCCCAGTTTTTGCAGATGGTGAAACCCCACGGCCCCCGCCGCACCTTTAAttt                                                                                                                                  <  2:1327844/149‑1 (MQ=255)                         ttCTACGTCGTGACGCCCCTCTTCTTTCATCTCTCCAACCCATTCCCCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGTTGTTGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTa                                                                                                                                <  2:506256/148‑1 (MQ=255)                               gtcgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGtccttcc                                                                                                                       >  2:316590/1‑151 (MQ=255)                               gtcgtGACCCCCCTCTTCTTTCATCTCTTCAACCCATCCCCCGACTTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGTTTTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGt                                                                                                                             <  1:1052159/145‑1 (MQ=255)                                   tGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTca                                                                                                                    >  1:50006/1‑150 (MQ=255)                                      cccctcttttcttTCATCTCTTCAATCCATCCCCCGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTca                                                                                                                    <  1:550413/139‑1 (MQ=255)                                      ccccccttttcttTCATCTCTACACTCCATCCCCCGACTTTATCTCCCCAGGCGAGAAGGTCAGGAGACTAAATTTTCCCACCCAGTTTTTGCAGTGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaac                                                                                                                  <  2:1359742/141‑1 (MQ=255)                                      ccccatttttcttcCATCTCTTCAATCCATTCCCCGCCGTTATCCCCCCAGGCGGAAAGGTCAGGAGACGAAATTTGCGGCCCCGGCTGTTGCAGAGGGATTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaaca                                                                                                                 <  1:1029418/142‑1 (MQ=255)                                        cctctctTTTTTCATCTCTACAATCCATCCACCGACGTTATCTTCCCAGGCGGAAAGGACAGGAGACAGAATTGGCTGCCCAAGCTGTTGAAGATGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATg                                                                                                              <  2:81973/148‑1 (MQ=255)                                         ccctctTCTTCCATCTCTTCAATCCATCCCCCGACTTTATCTCCCCAGGCCGGAGGGTCAGGGGCCTGAATTTCCTGCCCCAGTTTTTGCAGAGGGCTTACCCCCACGGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGc                                                                                                             <  2:940416/149‑1 (MQ=255)                                          aCTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGcc                                                                                                            >  1:240127/1‑151 (MQ=255)                                            tcttctTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtt                                                                                                          >  2:1236079/1‑151 (MQ=255)                                              ttttttCATCTCTCAAACCCATCCCCCGACTTTATCTCCCCAGGCGGGAAGGTCAGGAGCCTGATTTTGCTGACCCATTTTTTGCAGATGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttt                                                                                                         <  2:1318047/147‑1 (MQ=255)                                              ttcttTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttt                                                                                                        >  1:36112/1‑151 (MQ=255)                                              ttcttTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttt                                                                                                        >  2:771995/1‑151 (MQ=255)                                               tcttTCATCTCTTCAACCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGATTTTGCTGACCCAGCTGTGGCAGATGGCGTACCCCCACGGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttt                                                                                                        <  1:1170264/150‑1 (MQ=255)                                                ttttCCTCTCTCCTATCTATCCCCCGAGGTTTTCTCCCCGGCCCGGATGTTCAGAGGATTGATTTTGCTGACCAAGCTTTTGTAGATGGTTTAACCCCACTCCCACCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttt                                                                                                        <  2:769668/148‑1 (MQ=255)                                                cttTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttttt                                                                                                      >  1:1288314/1‑151 (MQ=255)                                                 tttCATCTCTTCATTCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGCCAGGAGACTGATTTTGCTGACCCATTTTTGGCAGATGGCGAACCCCCTCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtt                                                                                                          <  2:1047269/146‑1 (MQ=255)                                                  tccacctttccAACCCATCCCCCGACTTTTTCTTCCCAGGCCGAAAGGCCAGGCGTTTTATTTTGCGAACCCTACTTTGGCAGAGGAGGTACCCCCTCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTa                                                                                                     <  2:1323001/140‑1 (MQ=255)                                                           tCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCg                                                                                           >  1:9619/1‑151 (MQ=255)                                                            cAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCg                                                                                           >  1:413665/1‑150 (MQ=255)                                                             aacccATCCCCCGTCTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGATTTTTCTGCCCCAGTTTTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcg                                                                                              <  2:1216715/143‑1 (MQ=255)                                                             aacccATCCCCCGACTTTATCTCCCCAGGCGGGAGGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTAt                                                                                                    <  1:661667/137‑1 (MQ=255)                                                               tCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTc                                                                                         >  1:1031658/1‑149 (MQ=255)                                                               cccAATCCCCGACGTATTCTCCCCCGGCCGGAAGGCAAGGAACCTAATTTTCCGCCCCCAGCTTTGAAAGATGGCTTACCCCCACGCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTg                                                                                                <  2:95666/141‑1 (MQ=255)                                                                ccttcccccGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGCCTGAATTTCCTGCCCCAGTTGTTGCAGATGGCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCa                                                                                        <  1:42073/142‑1 (MQ=255)                                                                ccATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTc                                                                                         >  2:1080439/1‑148 (MQ=255)                                                                ccATCCCCCGCCTTTTTCTCCCCAGGCCGGAAGGCCAGGAGACTGAATTTCCTCCCCCAGTTTTTGAAGATGGCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTc                                                                                         <  2:1106017/148‑1 (MQ=255)                                                                ccATCCACCGCCTTTTTCTTCCCAGGCGGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCa                                                                                        <  1:1056747/149‑1 (MQ=255)                                                                    tCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTc                                                                                   >  2:888373/1‑150 (MQ=255)                                                                              tATCTCCCCGGGCGGGAAGGTCAGGAGACTGAAGTTCCGGCCCCTGTTGTTGCAGTTGGCCAACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCa                                                                            <  2:213969/147‑1 (MQ=255)                                                                                tctcCCCGGCCCGGAAGCTCAGGAGACTAATTTTCCCGCCCAAGTTGTTGCAGAGGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCa                                                                         <  1:1258486/148‑1 (MQ=255)                                                                                     ccAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGAc                                                                  >  1:1146060/1‑150 (MQ=255)                                                                                      cAGGCCGGAAGGTCAGGAGACTGAATTTGCTCACCCAGTTGTTGCAGATGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                                <  2:655004/151‑1 (MQ=255)                                                                                       aGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                                >  2:1321761/1‑150 (MQ=255)                                                                                        ggCCGGAGGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATa                                                               <  1:957093/150‑1 (MQ=255)                                                                                        ggCCGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                                <  1:99388/149‑1 (MQ=255)                                                                                         gCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGc                                                             >  2:523993/1‑151 (MQ=255)                                                                                          ccGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGcc                                                            >  1:252181/1‑151 (MQ=255)                                                                                           cGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGccc                                                           >  1:901437/1‑151 (MQ=255)                                                                                            ggAGGGTCAGGAGACTGAATTTGCTGCCCCGTCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAg                                                              <  1:455541/147‑1 (MQ=255)                                                                                            ggAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCg                                                          >  1:796650/1‑151 (MQ=255)                                                                                            ggAAGGTCAGGAGACTAATTTTGCTGCCCCAGTTGTTGCAGATGGCGTACCCCCCCGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCg                                                          <  1:820367/151‑1 (MQ=255)                                                                                                 gTCAGGAGACTAATTTTGCAGACCCGTTTGTTGAAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatc                                                     <  1:536918/151‑1 (MQ=255)                                                                                                  tCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatc                                                     >  1:1282806/1‑150 (MQ=255)                                                                                                      gagaCTGATTCTCCTAACCCAGCTGTTGCAGAGGGCGTAACCCCGCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatca                                                    <  1:722884/147‑1 (MQ=255)                                                                                                       agaCTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttc                                               >  1:670756/1‑151 (MQ=255)                                                                                                        gaCTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttct                                              >  2:890770/1‑151 (MQ=255)                                                                                                        gaCTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttct                                              >  1:104163/1‑151 (MQ=255)                                                                                                         aCTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTtctc                                             >  1:399329/1‑151 (MQ=255)                                                                                                           tGAATTGGCGCACCCAGCTGTTGCAGAGGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttc                                               <  1:254063/147‑1 (MQ=255)                                                                                                            gAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaa                                           >  1:1208217/1‑150 (MQ=255)                                                                                                            gAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaa                                           >  2:1331456/1‑150 (MQ=255)                                                                                                              aTTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAac                                         >  2:447932/1‑150 (MQ=255)                                                                                                               ttcgcggccccATCTGTTGAAGGTGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaa                                           <  1:64864/139‑1 (MQ=255)                                                                                                                ttGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAacac                                       <  1:1108147/150‑1 (MQ=255)                                                                                                                    tGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                                   >  2:414016/1‑150 (MQ=255)                                                                                                                     gACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTa                                  >  2:543894/1‑150 (MQ=255)                                                                                                                                   aGATGGCGTACCCCCCCTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                    <  1:1289181/150‑1 (MQ=255)                                                                                                                                       ggCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCt                <  1:971978/150‑1 (MQ=255)                                                                                                                                        gattAACCCCACGCACCCCGCCGCTCCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCa                   <  1:352954/143‑1 (MQ=255)                                                                                                                                        gCGTAACCCCACTGACCCCGCCACACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCtt               >  1:455153/1‑150 (MQ=255)                                                                                                                                            acccccACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCAGCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTc          <  1:592678/149‑1 (MQ=255)                                                                                                                                            aaCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTc          >  2:1077967/1‑151 (MQ=255)                                                                                                                                              ccccACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGt           <  2:886792/148‑1 (MQ=255)                                                                                                                                              ccccACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCt        >  2:863473/1‑151 (MQ=255)                                                                                                                                                 cACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACa     <  1:832641/151‑1 (MQ=255)                                                                                                                                                   ccgACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACaa    <  1:817293/148‑1 (MQ=255)                                                                                                                                                    tGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGt  >  1:899990/1‑151 (MQ=255)                                                                                                                                                     gACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGt  >  2:636690/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                     TTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGT  >  NC_000913/3350704‑3351001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑