Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,332,791	0	A	C	42.5%	163.5 / 21.9	87	V161G (GTG→GGG)	basS	sensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base A (46/4);  new base C (0/37);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 2.01e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGG  >  NC_000913/4332643‑4332939                                                                                                                                                       |                                                                                                                                                     aTGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCAcc                                                                                                                                                    >  1:155218/1‑151 (MQ=255)     tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCAccccc                                                                                                                                                 <  2:351721/150‑1 (MQ=255)     tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCAccccc                                                                                                                                                 <  1:463227/150‑1 (MQ=255)     tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCg                                                                                                                                                >  1:1040947/1‑151 (MQ=255)      gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGc                                                                                                                                               >  1:276773/1‑151 (MQ=255)         aaTTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGcc                                                                                                                                              >  2:644010/1‑149 (MQ=255)         aaTTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGcc                                                                                                                                              >  2:402436/1‑149 (MQ=255)            tACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTgg                                                                                                                                         >  2:428586/1‑151 (MQ=255)             aCCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTg                                                                                                                                          >  2:476013/1‑149 (MQ=255)              ccGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCg                                                                                                                                       >  1:1209255/1‑151 (MQ=255)              ccGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCg                                                                                                                                       >  1:674085/1‑151 (MQ=255)                  aagaaAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GCTGCAAA‑CGCACCCCCGCCAGTGGCGTTCg                                                                                                                                   >  2:1018648/1‑151 (MQ=255)                  aagaaAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCg                                                                                                                                   >  1:1213152/1‑151 (MQ=255)                    gaaAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGc                                                                                                                                  >  1:970599/1‑150 (MQ=255)                       aatgcttGTCCGGCGCGCGCAATTGGCAGCAGCGGGGAGCCGCTCTCCATCATCTGATCAAGCCGTGCCACTGACGGTGCTACATCAATGTGATGCTTTTTCGCCAGCATTCCCA‑GATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGtt                                                                                                                              <  1:934869/145‑1 (MQ=255)                       aaTGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGt                                                                                                                               >  2:384985/1‑150 (MQ=255)                            cTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTgc                                                                                                                         >  1:331996/1‑151 (MQ=255)                            cTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTgc                                                                                                                         >  1:750701/1‑151 (MQ=255)                             tgcccGGCCCGCCCCAGTGGCAGCAGCGGGGAGCCCCTCCCCATCTCCTGATCAAGCCGTCCCCCTACGGGTGCTACATCAATGTGATGTGTTTTCCCCAGCAGTTCCA‑GATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGt                                                                                                                           <  2:1038248/145‑1 (MQ=255)                             tGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTgcg                                                                                                                        >  2:300508/1‑151 (MQ=255)                              gccgggCACGCGCTAGTTGCGGCGGTTGGGAGACGCTCCCCATAATCGGACCAGGCCGTCCCACTACCGGGGTTACATCAATGTGATGCTTTCCCGCCAGCATTCCCA‑TACGAAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTgcgc                                                                                                                       <  2:59267/147‑1 (MQ=255)                                ccGCCACCCGCCGGTTGCAGCGGCGGGGAGCCGCTCTCCATCTTCTGATCAAGCCGTCCCATTAACGGTGCTACATCAATGTGTTGCTTTTCCGCCAGCAGTTCCA‑GATGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTgc                                                                                                                         <  1:287645/147‑1 (MQ=255)                                  ggCCCGCGCCGTTGGCAGCAGGGGGGGGCCCCTCCCCATCTTTTGACCAGGCCGCGCCACTACGGGTGCTCCATCAGTGTGTTGCTTTTCCGCCAGCAGTCCCA‑GATCAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGAc                                                                                                                    <  2:1069716/150‑1 (MQ=255)                                    cACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc                                                                                                                 >  2:307818/1‑151 (MQ=255)                                          ccAGTGGCAGCAGCGGGGAGCCGCTCCCCATCATCTGATCAAGCCGTCCCACTAGCGGTGCTACATCAATGTGATGCTTTTTCGCCAGCAGTTCCA‑GATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCg                                                                                                             <  1:126220/149‑1 (MQ=255)                                          ccAGTGGCAGCAGCGGGGAGCCCCTCCCCATCATCTGACCAAGCCGTGCCACTACCGGTCCTACAACAATGTGATGCTTTTTCCCCAGCAGTTCCA‑GAGGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGc                                                                                                              <  1:777197/148‑1 (MQ=255)                                           cAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGt                                                                                                           >  2:1290615/1‑150 (MQ=255)                                                gcagcaGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCACCAGCAGTTCAA‑GATGCAAA‑CACACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaaca                                                                                                      >  2:166389/1‑150 (MQ=255)                                                 cagcagCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaac                                                                                                    >  1:27034/1‑151 (MQ=255)                                                 cagcagCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaa                                                                                                     >  1:572353/1‑150 (MQ=255)                                                    cagcTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCtt                                                                                                 >  1:680376/1‑151 (MQ=255)                                                    cagcTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCtt                                                                                                 >  2:1081406/1‑151 (MQ=255)                                                      ggtgggggcccctctCCACCATCTGACCAGCCCGGCCCACAAACGGTGCTAAATCAAGGTGATGGGTTTTCGCCAGCGTTTCCA‑GAGGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTa                                                                                                          <  2:81847/129‑1 (MQ=255)                                                      ggtGGGAGCCCCTCCCCACCCTTTAAACACGCCGGCCCCCTAACGGTGCTACATAATTGTGATGTTTTTTCCCAAGCATTTCCA‑GATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTc                                                                                               <  2:423829/149‑1 (MQ=255)                                                      ggggggAGCCCCTCCCCATCATCTGAACAGGCCGTCCCACTACCGGTGCTACATAAAGGTGGTGCTTTTTCGCCAGCAGTTCAA‑GATGAAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCtt                                                                                                 <  2:1196253/146‑1 (MQ=255)                                                      gcTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTc                                                                                               >  1:1352504/1‑151 (MQ=255)                                                        tGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGt                                                                                             >  1:1275966/1‑151 (MQ=255)                                                        ggggAGCCCCTCCCCATCATCTGACCAAGCCGTGCCACTACGGGTGCAACATAATTGTGATGTTTTTTCCCCAGCATTTCCA‑GATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGt                                                                                             <  2:1109451/150‑1 (MQ=255)                                                         gggAGCCGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATAAATGTGATGCTTTTTCGCCAGCAGTTCAA‑GATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCttt                                                                                                <  1:148983/147‑1 (MQ=255)                                                         gggAGCCCCTCTCCATCATCTGACCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCTTTTCCGCCAGCAGTTCCA‑GATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCg                                                                                              <  1:1358675/149‑1 (MQ=255)                                                         gggAGCCCCTCCCCATCATCTGATCAAGCGGTGCCACAACCGGTGCTACATCAATGTGTTGCTTTTCCGCCAGCAGTTCCA‑GATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaa                                                                                                     <  1:778472/142‑1 (MQ=255)                                                               ccctctCCATCATCTGATCAGGCCGTGCCTCTAAGGGTGCTACATCAATGTGTTGCTTTTTCGCCAGCAGTCCCA‑GATGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg                                                                                      <  1:1290643/149‑1 (MQ=255)                                                                cctccCCATCATTTGACAAACCCGTGCCACTAACGGTGCTACATAAATGTGATGCGTTTCCCCCAGCAGTTCAA‑GATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGc                                                                                     <  1:451705/150‑1 (MQ=255)                                                                  tctcCCTCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGGAAACAACCTTTCGTTATCCAGCg                                                                                    >  2:965395/1‑150 (MQ=255)                                                                  tccccATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATAAATGTGATGCTTTTTCGCCAGCAGTTCCA‑GATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCa                                                                                       <  1:640455/144‑1 (MQ=255)                                                                   ctcctccttcgaaccAAGCCGTCCCCCTAGCGGTGCTCCACAAATGTGTTGTTTTTCCCCCAGCAGTCCCA‑GATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCa                                                                                       <  2:680516/132‑1 (MQ=255)                                                                   ctcCATCCTTTAACCAAGCCGTGCCACTAACGGTGCTCCATCAATTTGTTGCGTTTCCGCCAGCAGTTCCA‑GATGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCg                                                                                    <  2:1210364/149‑1 (MQ=255)                                                                    cccacatacTGATCACGCCGTGCCACAAACGGTCCTACATAAATGGAATTTTTTTTCGCCAGCTTTTCGG‑GAGCAAAA‑CCCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGc                                                                                 <  2:750399/143‑1 (MQ=255)                                                                     ccATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCt                                                                                >  2:841011/1‑151 (MQ=255)                                                                     ccATCATCGAACCAAGCCGGGCCCCTAACGGTGCTACATAAATGTGATGCTTTTCCGCCGGCGGTTCCA‑GA‑GCAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCt                                                                                <  2:84611/151‑1 (MQ=255)                                                                         catcTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTc                                                                            >  2:528244/1‑151 (MQ=255)                                                                             tGATAAACCCGTGCCACTACGGGTGTTACATAATGGTGATGCGTTTGCGCCGGCAGTCCCA‑GAGGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCa                                                                           <  2:1189299/148‑1 (MQ=255)                                                                                tCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGAc                                                                     >  2:391533/1‑151 (MQ=255)                                                                                   aGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg                                                                   >  2:909281/1‑150 (MQ=255)                                                                                     ccgccccACTACCGGTGCTACATCAATGTGAGGCTTTTCCGCCAGCAGTCCCA‑GATCCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGa                                                                  <  1:476633/144‑1 (MQ=255)                                                                                       gtcccACTAACGGTGCTACATCAATGTGATGCGTTTCCGCCAGCAGTTCCA‑GATGCAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCa                                                               <  1:1106701/147‑1 (MQ=255)                                                                                       gtcccACAAACGGGGCTACAAAAAGGTGATGC‑TTTTCCCCCGGATGTCCAGGACGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAg                                                                          <  2:1040926/136‑1 (MQ=255)                                                                                          ccacaacgggTCCTACATAAATGTGATGCGTTTCCGCCAGCAGTCCCA‑GATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATc                                                           <  2:1333301/143‑1 (MQ=255)                                                                                          ccACTAACGGTGCTACATCAATGTGATGCTTTTCCGCCGGCATTTCCA‑GATGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGAt                                                            <  2:632514/150‑1 (MQ=255)                                                                                           cACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                                >  1:302948/1‑145 (MQ=255)                                                                                            aCTAACGTTGCTACAGAAATGTGATGCTTTTTCGCCAGCATTTCCA‑GATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATc                                                           <  1:1147201/149‑1 (MQ=255)                                                                                               aaCGGTGCTACATCAATGTGATGCTTTTCCGCCAGCGGTCCCA‑GATACAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTa                                                      <  1:367843/151‑1 (MQ=255)                                                                                               aaCGGGGCCACATCAAGGTATGGGGTTTTCCCGAGCAGTCCCA‑GAGGCAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGtt                                                        <  1:162597/149‑1 (MQ=255)                                                                                                aCGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTa                                                      >  1:1185144/1‑150 (MQ=255)                                                                                                     gCTACATAAATGTGATGCTTTTCCGCCAGCAGTTCCA‑GATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGcc                                                 <  1:625967/150‑1 (MQ=255)                                                                                                       tACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGa                                               >  1:369398/1‑150 (MQ=255)                                                                                                       tACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGCTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCg                                                >  2:1345348/1‑149 (MQ=255)                                                                                                        aCATCAATGTGATGCTTTTTCGCCAGCAGTCCCA‑GATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCg                                                <  1:402443/148‑1 (MQ=255)                                                                                                        aCATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaa                                              >  1:913517/1‑150 (MQ=255)                                                                                                         cATCAGTGTGATGTTTTTCCGCCAGCTGTTCCA‑GATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaa                                              <  2:704680/149‑1 (MQ=255)                                                                                                           tCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacc                                           >  2:404387/1‑150 (MQ=255)                                                                                                                 tgATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCtt                                    >  1:1175309/1‑151 (MQ=255)                                                                                                                  gagggttttcCGCCAGCAGTTCCA‑GATGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGc                                      <  2:1321530/142‑1 (MQ=255)                                                                                                                       ttttcccccggcagTCCAA‑GAAGCAAC‑CCCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCtt                                    <  2:1209230/135‑1 (MQ=255)                                                                                                                         tttCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCg                            >  1:1095783/1‑151 (MQ=255)                                                                                                                          ttCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCg                            >  1:65546/1‑150 (MQ=255)                                                                                                                          ttCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCg                            >  1:1179225/1‑150 (MQ=255)                                                                                                                            cGCCAGCAGTCCCA‑GATACAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGa                           <  1:480703/149‑1 (MQ=255)                                                                                                                               cagcagTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTg                      >  2:693900/1‑151 (MQ=255)                                                                                                                                agcagTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTgg                     >  1:544265/1‑151 (MQ=255)                                                                                                                                     tcccA‑GATACAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                 <  1:46547/148‑1 (MQ=255)                                                                                                                                      tCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgt               >  1:133321/1‑151 (MQ=255)                                                                                                                                       caa‑GATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgt               <  2:165668/148‑1 (MQ=255)                                                                                                                                               cAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGc        >  2:1247425/1‑150 (MQ=255)                                                                                                                                                 aaccGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATgg         <  1:899400/145‑1 (MQ=255)                                                                                                                                                  a‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATg    >  1:995546/1‑151 (MQ=255)                                                                                                                                                     gCACCCCCGCCAGTGGCGTTCGCAGTTC‑TGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGGGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGGGGCGCTGTGAATGGCAATggg  >  1:532833/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     ATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCA‑GATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGG  >  NC_000913/4332643‑4332939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑