Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,212	T→C	E273G (GAA→GGA)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,212	0	T	C	100.0%	328.8 / NA	83	E273G (GAA→GGA)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/52);  total (31/52)

TGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCC  >  NC_000913/3440064‑3440358                                                                                                                                                     |                                                                                                                                                   tGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCt                                                                                                                                                  >  2:202287/1‑151 (MQ=255)  ggCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCt                                                                                                                                                  <  1:777948/150‑1 (MQ=255)      aGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCttt                                                                                                                                             <  2:408756/151‑1 (MQ=255)       gTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTa                                                                                                                                            <  2:402804/151‑1 (MQ=255)            ccAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCa                                                                                                                                       <  1:193132/151‑1 (MQ=255)             cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGc                                                                                                                                        >  2:325274/1‑149 (MQ=255)                gcgcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTa                                                                                                                                   <  1:1035726/151‑1 (MQ=255)                 cgcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAg                                                                                                                                  <  1:385817/151‑1 (MQ=255)                      gCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCagag                                                                                                                             <  2:750982/151‑1 (MQ=255)                        ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCg                                                                                                                           <  1:251543/151‑1 (MQ=255)                         cAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCagag                                                                                                                             <  1:212435/148‑1 (MQ=255)                          agagACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCgg                                                                                                                          >  1:884513/1‑150 (MQ=255)                           gagaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGAc                                                                                                                        <  1:1265214/151‑1 (MQ=255)                              aCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGt                                                                                                                     >  2:6789/1‑151 (MQ=255)                                aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCa                                                                                                                   <  1:296590/151‑1 (MQ=255)                                 gTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCaa                                                                                                                  <  1:266980/151‑1 (MQ=255)                                      cGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                                                                                                              >  1:424390/1‑150 (MQ=255)                                      cGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCa                                                                                                             <  1:648119/151‑1 (MQ=255)                                       gggAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGGACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAg                                                                                                            <  2:1166041/151‑1 (MQ=255)                                        ggAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGa                                                                                                           <  2:658498/151‑1 (MQ=255)                                         gAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCGACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGAt                                                                                                          <  1:910343/151‑1 (MQ=255)                                         gAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                                                                                                              >  1:1115246/1‑147 (MQ=255)                                         gAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGAt                                                                                                          <  1:966312/151‑1 (MQ=255)                                          aaGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATc                                                                                                         <  2:1261619/151‑1 (MQ=255)                                           aGCCAGCACTTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCACCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGAt                                                                                                          <  2:998232/149‑1 (MQ=255)                                           aGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCg                                                                                                        <  1:617437/151‑1 (MQ=255)                                            gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGt                                                                                                       >  1:392001/1‑151 (MQ=255)                                            gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGt                                                                                                       >  2:297726/1‑151 (MQ=255)                                            gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGt                                                                                                       <  1:398165/151‑1 (MQ=255)                                             ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTc                                                                                                      <  1:1289145/151‑1 (MQ=255)                                              cAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCa                                                                                                     <  2:58553/151‑1 (MQ=255)                                                         tAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcgc                                                                                            >  1:337732/1‑149 (MQ=255)                                                          aaTCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgcag                                                                                          <  1:602690/150‑1 (MQ=255)                                                             ctcAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAgg                                                                                      >  2:1306012/1‑151 (MQ=255)                                                               cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGcagcag                                                                                       <  2:172749/148‑1 (MQ=255)                                                               cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggat                                                                                    <  2:697902/151‑1 (MQ=255)                                                                   aaGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgga                                                                                <  1:252732/151‑1 (MQ=255)                                                                   aaGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgg                                                                                 >  1:1094297/1‑150 (MQ=255)                                                                     gagaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggatc                                                                              <  1:1355922/151‑1 (MQ=255)                                                                      agaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGgatcggatcg                                                                             <  1:724496/151‑1 (MQ=255)                                                                           ttttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAAct                                                                         <  1:221155/150‑1 (MQ=255)                                                                            tttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActc                                                                        >  2:750455/1‑150 (MQ=255)                                                                              tACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTgg                                                                     >  1:447467/1‑151 (MQ=255)                                                                               aCCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGt                                                                    >  1:543371/1‑151 (MQ=255)                                                                                ccAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                                                                   <  2:1073700/151‑1 (MQ=255)                                                                                  aaGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtc                                                                 >  2:8340/1‑151 (MQ=255)                                                                                  aaGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtc                                                                 >  2:1251659/1‑151 (MQ=255)                                                                                   aGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                                >  1:389360/1‑151 (MQ=255)                                                                                   aGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtc                                                                 <  2:1045087/150‑1 (MQ=255)                                                                                    ggTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtctc                                                               >  2:1175863/1‑151 (MQ=255)                                                                                        aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttct                                                           <  1:225667/151‑1 (MQ=255)                                                                                         ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTcttctt                                                          <  2:864343/151‑1 (MQ=255)                                                                                             ttttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCAc                                                      >  1:1161732/1‑151 (MQ=255)                                                                                              tttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCAc                                                      <  2:1054906/150‑1 (MQ=255)                                                                                              tttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACt                                                     >  1:285666/1‑151 (MQ=255)                                                                                                 aaGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCa                                                  <  2:236026/151‑1 (MQ=255)                                                                                                    gAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGc                                               <  2:337483/151‑1 (MQ=255)                                                                                                     aGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCt                                              <  2:498479/151‑1 (MQ=255)                                                                                                     aGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCt                                              >  1:446863/1‑151 (MQ=255)                                                                                                         cAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGAc                                           <  2:33554/150‑1 (MQ=255)                                                                                                          aaCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGt                                         >  2:635529/1‑151 (MQ=255)                                                                                                           aCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGt                                         >  1:388090/1‑150 (MQ=255)                                                                                                                ggTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACAt                                     <  2:382260/149‑1 (MQ=255)                                                                                                                 gTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCAc                                  <  1:608509/151‑1 (MQ=255)                                                                                                                 gTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCAc                                  <  1:13303/151‑1 (MQ=255)                                                                                                                   aCGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGt                                <  2:1076382/151‑1 (MQ=255)                                                                                                                   aCGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAAGCTGACGTACATCACGt                                <  1:543719/151‑1 (MQ=255)                                                                                                                       tGTACCAGATCACCGATATAGTGGATAGATCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGt                            >  2:678544/1‑151 (MQ=255)                                                                                                                        gTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGt                            <  1:920047/150‑1 (MQ=255)                                                                                                                           ccAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAAc                        >  2:424791/1‑151 (MQ=255)                                                                                                                           ccAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGAGCGAACTCTGGTTGCTCTTCTTTCACATCAGGCTGACGTACATCACGTAAGTCAAc                        <  2:302524/151‑1 (MQ=255)                                                                                                                             aGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGa                      <  1:693867/151‑1 (MQ=255)                                                                                                                                 cACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGc                  >  2:572660/1‑151 (MQ=255)                                                                                                                                     gATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc              >  2:607652/1‑151 (MQ=255)                                                                                                                                     gATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc              <  2:337726/151‑1 (MQ=255)                                                                                                                                        ataGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgt           <  1:641510/151‑1 (MQ=255)                                                                                                                                         taGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgtt          >  2:1341971/1‑151 (MQ=255)                                                                                                                                           gTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttgt        >  1:109458/1‑151 (MQ=255)                                                                                                                                            tGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttgtt       >  2:452349/1‑151 (MQ=255)                                                                                                                                                 aGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGcc  >  1:171510/1‑151 (MQ=255)                                                                                                                                                 aGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGcc  <  2:918223/151‑1 (MQ=255)                                                                                                                                                 aGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGcc  <  1:451613/151‑1 (MQ=255)                                                                                                                                                 aGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGc   <  1:1306041/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCC  >  NC_000913/3440064‑3440358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑