Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,237	0	C	T	43.0%	107.0 / 38.1	86	S64L (TCG→TTG)	lsrD	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base C (5/44);  new base T (37/0);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 2.94e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTT  >  NC_000913/1604091‑1604385                                                                                                                                                   |                                                                                                                                                      cGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGttt                                                                                                                                                   <  1:16646/151‑1 (MQ=255) cGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGt                                                                                                                                                     <  2:1069700/149‑1 (MQ=255)    tATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTGGATATTTTGttt                                                                                                                                                   >  2:131493/1‑148 (MQ=255)    tATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTgg                                                                                                                                                 <  1:525107/150‑1 (MQ=255)        gagaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGACGATATTTTGttttg                                                                                                                                                 >  1:947461/1‑144 (MQ=255)        gagaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTGGTTc                                                                                                                                              >  2:846188/1‑149 (MQ=255)        gagaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGtt                                                                                                                                               >  1:421168/1‑148 (MQ=255)        gagaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGa                                                                                                                                            <  2:215503/151‑1 (MQ=255)        gagaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGa                                                                                                                                            <  1:944508/151‑1 (MQ=255)         agaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTGTTTGGt                                                                                                                                                >  2:596092/1‑146 (MQ=255)                cGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTGTTTTGTTCTAcc                                                                                                                                          >  1:101610/1‑145 (MQ=255)                cGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCgg                                                                                                                                     <  2:1194772/150‑1 (MQ=255)                cGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGc                                                                                                                                    <  1:756555/151‑1 (MQ=255)                  cATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTCTTTTGTTTGACCATCGGcc                                                                                                                                   >  2:508283/1‑150 (MQ=255)                     ttGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCctct                                                                                                                                <  2:523632/150‑1 (MQ=255)                      tGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTGGTTTGACCCTCGGCctc                                                                                                                                 >  1:1115545/1‑148 (MQ=255)                       ggTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTgcg                                                                                                                             <  2:1179184/151‑1 (MQ=255)                            aaTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTGATATTTTTTTTTGTTCGACCCACGGCCTCCGCGcccct                                                                                                                        >  2:42575/1‑148 (MQ=255)                            aaTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcatt                                                                                                                        <  1:571061/151‑1 (MQ=255)                             aTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTTTTTTGTTCGGCCCACCACCTCTGCGCcctt                                                                                                                        >  2:97569/1‑150 (MQ=255)                             aTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTCGTTTTGTTTCGCCATCGGCCTCTCCGcc                                                                                                                           >  1:531212/1‑147 (MQ=255)                             aTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGAGATTTTTTTTTGGTTGAACATCGGCCCCTGCGCcctt                                                                                                                        >  2:992524/1‑150 (MQ=255)                             aTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCCGCCTCTTCGCcctt                                                                                                                        >  1:1237065/1‑150 (MQ=255)                              ttAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCcatt                                                                                                                        >  2:605526/1‑149 (MQ=255)                              ttAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTTGTTTTACCATCCGCCTCTTCGCcact                                                                                                                        >  1:1213252/1‑147 (MQ=255)                                      cgAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCg                                                                                                              <  2:20414/151‑1 (MQ=255)                                        aaTGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTGTTTTTTTCGACCATCGGCCTCTGCGCCATTGCATTTGGGGtg                                                                                                            >  2:974576/1‑150 (MQ=255)                                        aaTGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCATTGCATTtgg                                                                                                                >  1:1232594/1‑147 (MQ=255)                                              aGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTTGACCATCGGCCTCTTCGCCATTTCCTTGGGGGGACT‑Gttt                                                                                                     >  1:617046/1‑151 (MQ=255)                                                 tctcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCATTGCATTTGGCGTACT‑Gttt                                                                                                     >  1:525011/1‑148 (MQ=255)                                                  ctcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTGTTTGGTTTGGCCCTCGGCCTCTGCGCCATTTCCTTTGGGGGTCT‑Gttt                                                                                                     >  2:199286/1‑147 (MQ=255)                                                   tcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCCGTATTTTATTTTGGTCGTCCACAGGCCTCTGCGCCCAAGCAGTGGGCGGACTGGTTTCaa                                                                                                  >  1:462299/1‑150 (MQ=255)                                                    cAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCCTCGGCCCCTGCGCCCTTTCATTTGGCGGACT‑GTTTTAAAg                                                                                                >  1:703451/1‑150 (MQ=255)                                                    cAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTc                                                                                                    <  2:351709/146‑1 (MQ=255)                                                     aaTATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAgtg                                                                                              <  1:361313/151‑1 (MQ=255)                                                     aaTATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAgtg                                                                                              <  2:633813/151‑1 (MQ=255)                                                        atGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAgtggt                                                                                            <  2:679111/150‑1 (MQ=255)                                                          gTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAGCGGTGGTGCTTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCGTTGCATTGGGCGTACT‑GTTTCAAAGTGgtgt                                                                                          <  2:860260/150‑1 (MQ=255)                                                          gTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGgtgt                                                                                          <  1:1164013/150‑1 (MQ=255)                                                             gCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTTTTTGGGTTGACCCTCGGCCTCTGCGCCCTTTCATTGGGGGTACT‑GTTTTTAAGTGGggtg                                                                                         >  2:425599/1‑148 (MQ=255)                                                             gCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGATATTTTTTTTTGTTTCACCATCGGCCTCTGCGCCCTTGCATTGGGCGTTCT‑GTTTCTAAgagg                                                                                             >  2:195749/1‑144 (MQ=255)                                                                gTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGAt                                                                                    <  2:312117/150‑1 (MQ=255)                                                                gTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATg                                                                                   <  2:1002082/151‑1 (MQ=255)                                                                  tCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGc                                                                                  <  2:48971/150‑1 (MQ=255)                                                                      cACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCACCGGCCTCTGCGCCCTTGCATTTGGGGGACT‑GTTTCAAAAGGGTGTGCCGATGCCGc                                                                               >  2:1096450/1‑149 (MQ=255)                                                                       aCCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTCCTTTTGTTCCACCATCGGGCTCTGCGCCCCTGCATTGGGGGTACT‑TTTTTTAAGTGGTGTGCCGAAGccccccg                                                                            >  2:955919/1‑146 (MQ=255)                                                                        ccAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGc                                                                           <  1:251719/151‑1 (MQ=255)                                                                        ccAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCCATATTTTGTTTTGGTTGCCCCTCGGCCCCTGCGCCATTGCAATGGGGGTAAT‑GTTTCAAAGGGGGGGGCCGATGCCGCCgg                                                                            >  1:418867/1‑150 (MQ=255)                                                                           gTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCCCTTGCATTGGGGGGACT‑GTTTTAAAGAGGTGTGGCGGTGCCGCCGGCg                                                                          >  1:1135473/1‑149 (MQ=255)                                                                                 ttATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTTGACCATCCGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGGCGATGCCCCCGGCGGTACTcc                                                                   >  2:581082/1‑150 (MQ=255)                                                                                 ttATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTcc                                                                   <  2:362922/150‑1 (MQ=255)                                                                                   aTCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGAGCGATATTTTGTTTTTTTCCACCCTCCGCCCCCGCGCCCTTGCCATGGGGGTGCT‑GTTTTTAAATGGTGTGGCCCTGCCCCTGGCGGTACTcc                                                                   >  2:621261/1‑148 (MQ=255)                                                                                    tCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATCTTTTGTTTTGTTTGACCCTTGGCCCCTGCGCCATTGCATTGGGGGGACT‑GTTTCCAAAAGGGGTGGCGGTGCCGCTGGCGGTAATCCTgcc                                                               >  2:929079/1‑149 (MQ=255)                                                                                      tGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGATATTTTGTTTGGTTCGACCCTCGGCCTCTGCGCCCTTGCATTGGGCGTACT‑GTTTTTAAGTGGTGTGCCGATGCCGCTGGCGGTACTcccgccc                                                              >  2:39202/1‑145 (MQ=255)                                                                                        cattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTTTTTAGTTTGACCCTCCGCCTCCGCCGCCTTTTGTTGGGGGGGAT‑GTTTTAAAGGGGGGTGCCGGTGCAGCCGGGGTGACTCCTGACCttt                                                           >  2:551856/1‑150 (MQ=255)                                                                                        cattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTa                                                           <  1:139054/151‑1 (MQ=255)                                                                                         attggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGAGATTTTGTTTGGTTCGCCCCTCCGCCTCCCGGCCCCTGCCTTGGGCGGACT‑GTTTTAAAGTGGGGTGCCGGAGCCGCTGGCGGTACTCCCGa                                                                >  2:1129367/1‑145 (MQ=255)                                                                                            ggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTg                                                        <  2:785700/150‑1 (MQ=255)                                                                                                ttgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGGCCCCTTCGCCCTTGCCTTTGGGGTACT‑GTTTTAAAATGGGGTGGCGATGCCGCTGGCGGTACTCCTTACCTTTCTGCTCgg                                                   >  1:712886/1‑151 (MQ=255)                                                                                                  gTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGgc                                                  <  2:991454/150‑1 (MQ=255)                                                                                                     gCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCCGGCTCTGCGCCCTTTCCTTTGGCGTACT‑GTTTTAAAGAGGGGGGCCGATGCCCCCGGCGGTAATCCTGACCCTTCTGCTCgg                                                   >  1:810901/1‑146 (MQ=255)                                                                                                      cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCCCTTGCATTGGGCGTACT‑GTTTCAAAGGGGGGTGCCGATGCCGCTGGCGGTACTCCTGACCTTACTGCTCGGCGCCtt                                             >  1:292460/1‑151 (MQ=255)                                                                                                       ccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCCCTTGCATTGGGCGTACT‑TTTTTAAAGTGGGGTGCCGATGCCCCCGGCGAGACTCCTGCCCTTACTGCTCGGCGCCtt                                             >  2:945228/1‑150 (MQ=255)                                                                                                          aCCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtgtg                                          <  2:1115531/150‑1 (MQ=255)                                                                                                          aCCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtgtg                                          <  1:792593/150‑1 (MQ=255)                                                                                                          aCCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtgt                                           <  1:916179/149‑1 (MQ=255)                                                                                                            cGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCgg                                       <  1:299787/151‑1 (MQ=255)                                                                                                                aaCGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCt                                    <  2:568637/150‑1 (MQ=255)                                                                                                                  cGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGTTGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGAt                                 <  2:1011340/151‑1 (MQ=255)                                                                                                                  cGATCGTGAGTGTCAGGGGCGGGATCGATATTTCGTTTGGTTCGACCATCCGCCTCCGCGCCTTGGCATTGGGCGTACT‑GTTTCAAAGCGGTGTGCCGATGCCGCTGGCGATACTCATGACCTTACTGCTCGGCGCATTGTGCgggat                                    <  2:53398/148‑3 (MQ=255)                                                                                                                    aTGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCa                               <  2:48188/151‑1 (MQ=255)                                                                                                                       gTGATTGTCAGTGGCGGGATCCGAATTTTTCTTTGTTTGAACATTGGGCTCCTCGCCATTCTGTATGGGGTACT‑GTTTTTTAGTGGGGGTGCGATGCCGCTGGGGAAAAGCCTGACCCTACTGCTCCGGGCAGTGTTCGGGGTGAtgcaa                             >  2:480669/1‑146 (MQ=255)                                                                                                                         gATTGTCAGTGGCGGGAACGATATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCATTTCATTGGGCGGACT‑GTTTTAAAGTGGTGTGCCGATGCCGCCGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAAcccc                          >  1:329340/1‑151 (MQ=255)                                                                                                                          aTTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCg                         <  1:199289/151‑1 (MQ=255)                                                                                                                             gTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGa                       <  1:216006/150‑1 (MQ=255)                                                                                                                              tCAGTGGCGGGATCGATATTTTTTTTTGTTTGAACATCGGCCCCTGCGCCCATGCATTGGGGGTACT‑GTTTTAAAGAGGGGTGGCGATGCCGCCGGCGGTACTCCTGCCCCTTCTGCTCGGCGCCATGTGCGGGGTGGTTAACGCCGGgtt                     >  2:932028/1‑151 (MQ=255)                                                                                                                               cAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGatta                    <  1:508287/151‑1 (MQ=255)                                                                                                                                     cGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATc              >  2:1048646/1‑151 (MQ=255)                                                                                                                                      gggATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCt             <  2:1270470/151‑1 (MQ=255)                                                                                                                                      gggATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCt             <  1:416142/151‑1 (MQ=255)                                                                                                                                         aTCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCtata          <  2:372900/151‑1 (MQ=255)                                                                                                                                           cGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATAcc        <  1:871446/151‑1 (MQ=255)                                                                                                                                            gATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCa       <  2:617040/151‑1 (MQ=255)                                                                                                                                              tatTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATGCCaa      <  2:801063/150‑1 (MQ=255)                                                                                                                                               atTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCaaa     <  2:414107/150‑1 (MQ=255)                                                                                                                                                  tCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGtt  <  2:376163/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      CGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACT‑GTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTT  >  NC_000913/1604091‑1604385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑